Spectrophotometry, Glucose And Glycosylated Hemoglobin

20 Questions | Total Attempts: 62

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Spectrophotometry, Glucose And Glycosylated Hemoglobin

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Questions and Answers
  • 1. 
    Wavelength
    • A. 

      Distance between two successive peaks and expressed in terms of nanometers

    • B. 

      Distance between two successive troughs and expressed in terms of nanometers

    • C. 

      Distance between two successive peaks and expressed in terms of micrometers

    • D. 

      Distance between two successive troughs and expressed in terms of micrometers

  • 2. 
    Relationship of wavelength to frequency and energy
    • A. 

      The shorter the wavelength, the higher the frequency and energy

    • B. 

      The shorter the wavelength, the lower the frequency and energy

    • C. 

      The longer the wavelength, the higher the frequency and energy

    • D. 

      No relationship

  • 3. 
    LASER
    • A. 

      Light Amplification by Stimulated Emission of Radiation

    • B. 

      Light Amplification by Simulated Emission of Radiation

    • C. 

      Light Amplification by Simultaneous Emission of Radiation

    • D. 

      Light Amplification by Stimulated Emission of Radiofrequency

  • 4. 
    All are TRUE except:
    • A. 

      Hydrogen lamp: Infrared region

    • B. 

      Deuterium lamp: UV region

    • C. 

      Tungsten lamp: Visible region

    • D. 

      Silicone carbide: Infrared region

  • 5. 
    Accurate absorbance measurement requires a bandpass ______ the natural bandpass of the spectrophotometer
    • A. 

      > 1/5

    • B. 

      < 1/5

    • C. 

    • D. 

      > 1.5

  • 6. 
    Other term for Cuvet except
    • A. 

      Sample cell

    • B. 

      Absorption cell

    • C. 

      Analytical cell

    • D. 

      Adsorption cell

  • 7. 
    Beer's Law except
    • A. 

      Concentration of the unknown is proportional to absorbance

    • B. 

      Concentration of the unknown is inversely proportional to %transmittance

    • C. 

      Mathematically establishes the relationship between concentration

    • D. 

      Proportional to the inverse log of transmittance

  • 8. 
    Holmium oxide filter except
    • A. 

      Checks wavelength calibration

    • B. 

      Checks wavelength accuracy

    • C. 

      Checks wavelength indicated on the device

    • D. 

      Checks wavelength of light passed by the monochromator

  • 9. 
    Buffered sodium citrate additive
    • A. 

      Black top

    • B. 

      Red top

    • C. 

      Blue top

    • D. 

      Light blue top

  • 10. 
    Synthesized in the pancreas except
    • A. 

      Glucagon

    • B. 

      Insulin

    • C. 

      Somatostatin

    • D. 

      Cortisol

  • 11. 
    Type 1 diabetes except
    • A. 

      Autoantibodies (-)

    • B. 

      Childhood onset

    • C. 

      B-cells destruction

    • D. 

      Associated with HLA DR3 and DR4

  • 12. 
    Fasting requirement for random blood sugar testing
    • A. 

      8 hours

    • B. 

      10 hours

    • C. 

      0 hours

    • D. 

      6 hours

  • 13. 
    Inverse colorimetry employed by Hagedorn Jensen method
    • A. 

      Reduction of a yellow ferricyanide to a colorless ferrocyanide by glucose

    • B. 

      Reduction of a yellow ferrocyanide to a colorless ferricyanide by glucose

    • C. 

      Oxidation of a yellow ferricyanide to a colorless ferrocyanide by glucose

    • D. 

      Oxidation of a yellow ferrocyanide to a colorless ferricyanide by glucose

  • 14. 
    Part of glucose oxidase reagent that converts alpha-D glucose to Beta-D glucose
    • A. 

      Peroxidase

    • B. 

      Mutarotase

    • C. 

      Glacial HAC

    • D. 

      Hydrogen Peroxide

  • 15. 
    Reference method for glucose method
    • A. 

      Glucose oxidase method

    • B. 

      Hexokinase method

    • C. 

      Glucose dehydrogenase method

    • D. 

      Isotope Dilution Mass Spectrometry

  • 16. 
    End result of Neocuproine method
    • A. 

      Cuprous-Neocuproine yellow complex

    • B. 

      Cupric-Neocuproine yellow complex

    • C. 

      Cuprous-Neocuproine orange complex

    • D. 

      Cupric-Neocuproine orange complex

  • 17. 
    Added Plasma glucose after OGTT except
    • A. 

      30 mins: 30-60 mg/dL above fasting

    • B. 

      1 hour: 20-50 mg/dL above fasting

    • C. 

      2 hours: 40-70 mg/dL above fasting

    • D. 

      3 hours: fasting level or below

  • 18. 
    Standard glucose load recommended by WHO
    • A. 

      50 grams

    • B. 

      75 grams

    • C. 

      100 grams

    • D. 

      125 grams

  • 19. 
    Glycosylated hemoglobin except
    • A. 

      Largest subfraction of normal hemoglobin A in diabetic individuals only

    • B. 

      Reflects the average blood glucose level over the past 2-3 months

    • C. 

      Dietary status on the day of the test has no effect on the results on HbA1c

    • D. 

      Old RBCs and IDA reflect high HbA1c levels

  • 20. 
    Glucose except
    • A. 

      Only carbohydrate to be directly used for energy or stored as glycogen

    • B. 

      The presence of double bond and a negative charge in the enol anion makes glucose an active reducing substance

    • C. 

      Does not contain an active ketone or aldehyde group

    • D. 

      Brain is completely dependent on blood glucose for energy production

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