G3 Restriction Enzymes And Electrophoresis
10 Questions
| Total Attempts: 207
PRESENTER: G3 RESTRICTION ENZYMES AND ELECTROPHORESISPENYANGGAH: G3 RESTRICTION ENZYMES AND ELECTROPHORESIS
Questions and Answers
- 1.DNA termini without overhanging 3‘ or 5 ‘ ends.
- A.
Cohesive ends
- B.
‘Stickys’ ends
- C.
Palindromic sequences
- D.
Symmetrical sequences
- E.
Blunt ends
- 2.All restriction enzymes require ______ as cofactor
- A.
Ca2+
- B.
Mg2+
- C.
Mn2+
- D.
Pb2+
- E.
All of the above
- 3.To incorporate fragments of foreign DNA into a plasmid vector, methods for cutting and rejoining of ssDNA are required.
- A.
TRUE
- B.
FALSE
- 4.Restriction–modification systems occur in many bacterial species, and constitute a defense mechanism against the introduction of foreign DNA into the cell.
- A.
TRUE
- B.
FALSE
- 5.The sequence is the same, reading 5’→3’, on each strand.
- A.
Cohesive ends
- B.
‘Stickys’ ends
- C.
Palindromic sequences
- D.
Recognition sequences
- E.
Blunt ends
- 6.Restriction enzymes recognize sites ranging in size from 4 to 8 bp or more, and may give products with _________.
- A.
Protruding 5’ end
- B.
Protruding 3’ end
- C.
Blunt ends
- D.
A or B
- E.
A or B or C
- 7.The extremely high specificity of restriction enzymes for their sites of action allows large DNA molecules and vectors to be join reproducibly into defined fragments.
- A.
TRUE
- B.
FALSE
- 8.In fact, in some cases, DNA ends formed by enzymes with different recognition sequences may be compatible, provided the single-stranded tails can base-pair together.
- A.
TRUE
- B.
FALSE
- 9.Agarose is a protein derived from seaweed.
- A.
TRUE
- B.
FALSE
- 10.Agarose used for electrophoresis is a more purified form of the agar used to make bacterial culture plates.
- A.
TRUE
- B.
FALSE