G3 Restriction Enzymes And Electrophoresis

10 Questions | Total Attempts: 149

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G3 Restriction Enzymes And Electrophoresis

PRESENTER: G3 RESTRICTION ENZYMES AND ELECTROPHORESISPENYANGGAH: G3 RESTRICTION ENZYMES AND ELECTROPHORESIS


Questions and Answers
  • 1. 
    DNA termini without overhanging  3‘ or  5 ‘ ends.
    • A. 

      Cohesive ends

    • B. 

      ‘Stickys’ ends

    • C. 

      Palindromic sequences

    • D. 

      Symmetrical sequences

    • E. 

      Blunt ends

  • 2. 
    All restriction enzymes require ______ as cofactor
    • A. 

      Ca2+

    • B. 

      Mg2+

    • C. 

      Mn2+

    • D. 

      Pb2+

    • E. 

      All of the above

  • 3. 
    To incorporate fragments of foreign DNA into a plasmid vector, methods for cutting and rejoining of ssDNA are required.
    • A. 

      TRUE

    • B. 

      FALSE

  • 4. 
    Restriction–modification systems occur in many bacterial species, and constitute a defense mechanism against the introduction of foreign DNA into the cell.
    • A. 

      TRUE

    • B. 

      FALSE

  • 5. 
    The sequence is the same, reading 5’→3’, on each strand.
    • A. 

      Cohesive ends

    • B. 

      ‘Stickys’ ends

    • C. 

      Palindromic sequences

    • D. 

      Recognition sequences

    • E. 

      Blunt ends

  • 6. 
    Restriction enzymes  recognize sites ranging in size from 4 to 8 bp or more, and may give products with _________.
    • A. 

      Protruding 5’ end

    • B. 

      Protruding 3’ end

    • C. 

      Blunt ends

    • D. 

      A or B

    • E. 

      A or B or C

  • 7. 
    The extremely high specificity of restriction enzymes for their sites of action allows large DNA molecules and vectors to be join reproducibly into defined fragments.
    • A. 

      TRUE

    • B. 

      FALSE

  • 8. 
    In fact, in some cases, DNA ends formed by enzymes with different recognition sequences may be compatible, provided the single-stranded tails can base-pair together.
    • A. 

      TRUE

    • B. 

      FALSE

  • 9. 
    Agarose is a protein derived from seaweed.
    • A. 

      TRUE

    • B. 

      FALSE

  • 10. 
    Agarose used for electrophoresis is a more purified form of the agar used to make bacterial culture plates.
    • A. 

      TRUE

    • B. 

      FALSE

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