Growth media used in the smallest volume in which cells can grow.
Growth media designed to minimize the growth of contaminants.
Growth media that contains the minimal nutritional requirements for normal cells
a way to visualize new mutations in a minimum amount of time
used to reveal only mutant cell colonies against a dark background.
Only the plate supplemented with arginine
Only the plate lacking arginine.
The plate supplemented with arginine, but only if a mutation occurred.
They mixed strains A and B together.
They put strain A on one side of a U‐shaped tube and strain B on the other, with a filter between them.
They found bio- met- cells
They plated strain A and strain B separately onto minimal media plates to screen for spontaneous prototrophs
They observed mating bridges with transmission electron microscopes.
An F+ cell
An Fʹ plasmid
An Hfr cell
An F- cell
All except D
Far apart on the same chromosome
On different chromosomes.
Both B and D
Xyl mtl polA →
PolA mtl xyl →
mtl xyl polA →
Mtl polA xyl →
None of these
A and C
B and C
So that the plasmid can replicate in bacterial cells.
So that the plasmid can recombine with the bacterial genome.
To enable the recipient cell to conjugate
to provide a selectable marker
To map point mutations.
amino acid supplements
All of these
None of these
Are easily mutated
Are susceptible to infection by bacteriophage
Contain the F factor integrated in the bacterial chromosome.
have a low frequency of recombination
Less than a megabase in length.
compartmentalized within an intracellular membrane
Treating the cells chemically
Exposing cells to a strong electric field
Making the cell membrane more permeable to DNA
Allowing the culture to enter stationary phase growth
A, B, and C only
It can reproduce at warm or cool temperatures
It is required for transformation
It can be lytic or lysogenic
It causes bacterial cells to conjugate
It reproduces by binary fission
Replicates and the phage genes take over the bacterium
inserts itself physically into the host cellʹs chromosome
Expresses a repressor protein gene that inhibits the lytic pathway.
is replaced by a piece of bacterial DNA when packaged inside phage progeny.
Both B and C
A bacterial cell picks up two fragments of DNA from the environment, each with a different gene
double crossover occurs between prophage DNA and the bacterial chromosome.
Two genes are closely linked enough so that they can be packaged into a phage head and injected into a cell by a single phage
two genes are introduced into the same bacterium by simultaneous infection with two different phages
Both C and D