What Do You Know About Bacterial Conjugation? Trivia Facts Quiz

31 Questions | Total Attempts: 42

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What Do You Know About Bacterial Conjugation? Trivia Facts Quiz

What do you know about bacterial conjugation? Bacteria are very interesting to study, and in this special quiz, we get to understand the process through which genetic material moves through bacteria cells. Do give it a shot and be sure to check out other quizzes on bacteria, their importance, and how they affect other organisms. All the best!


Questions and Answers
  • 1. 
     ________ bacterial strains require nutritional supplements to grow in a minimal medium.
    • A. 

      Autotrophic

    • B. 

      Prototrophic 

    • C. 

      Phototrophic

    • D. 

      Heterotrophic

    • E. 

       Auxotrophic 

  • 2. 
     Minimal media is
    • A. 

      Growth media used in the smallest volume in which cells can grow. 

    • B. 

      Growth media designed to minimize the growth of contaminants. 

    • C. 

      Growth media that contains the minimal nutritional requirements for normal cells

    • D. 

       a way to visualize new mutations in a minimum amount of time

    • E. 

       used to reveal only mutant cell colonies against a dark background. 

  • 3. 
    An arg- strain of E. coli is transformed with a plasmid carrying the wild‐type (arg+) gene. The transformed cells are replica‐plated to two minimal medium plates: one supplemented with arginine and one lacking arginine. The transformed cells would grow on 
    • A. 

      Only the plate supplemented with arginine

    • B. 

      Only the plate lacking arginine.

    • C. 

      Both plates

    • D. 

      Neither plate

    • E. 

      The plate supplemented with arginine, but only if a mutation occurred. 

  • 4. 
    When Lederberg and Tatum performed their experiments on gene transfer in bacteria that lead to our understanding of conjugation, what were their controls?
    • A. 

      They mixed strains A and B together.

    • B. 

      They put strain A on one side of a U‐shaped tube and strain B on the other, with a filter between them. 

    • C. 

      They found bio- met- cells

    • D. 

      They plated strain A and strain B separately onto minimal media plates to screen for spontaneous prototrophs

    • E. 

       They observed mating bridges with transmission electron microscopes. 

  • 5. 
    A plasmid such as an F factor that is capable of integrating into the bacterial chromosome is a(n) 
    • A. 

      Prophage

    • B. 

      Episome. 

    • C. 

      Auxisome

    • D. 

       perisome

    • E. 

      Elaiosome. 

  • 6. 
    Which of the following has episomal DNA inserted in the cellʹs chromosome?
    • A. 

      An F+ cell 

    • B. 

      An Fʹ plasmid

    • C. 

      An Hfr cell

    • D. 

      An F- cell

    • E. 

       All except D

  • 7. 
    Cotransduction can be detected when the transduced genes are 
    • A. 

      Closely linked

    • B. 

      Far apart on the same chromosome

    • C. 

      On different chromosomes. 

    • D. 

      Mutant

    • E. 

       Both B and D 

  • 8. 
    Mtl, polA, and xyl are bacterial genes that can be transmitted using an Hfr strain. An interrupted mating experiment resulted in xyl exconjugants appearing at 7 minutes and reaching maximum level by 30 minutes. The mtl gene function exconjugants appeared at 11 minutes and leveled off at 80% maximum by about 33 minutes, while polA did not appear until 24 minutes into the experiment and then only slowly rose to about 30%. Which of the following shows the gene order with the insertion site (arrow) location correctly? 
    • A. 

      Xyl mtl polA → 

    • B. 

      PolA mtl xyl →

    • C. 

       mtl xyl polA → 

    • D. 

      Mtl polA xyl → 

    • E. 

      None of these 

  • 9. 
    Conjugation by which of the following with an F- cell results in a cell that remains F-? 
    • A. 

       F+ 

    • B. 

      Hfr

    • C. 

       F

    • D. 

      A and C

    • E. 

      B and C

  • 10. 
    The reason that many plasmids used in laboratories for transformation experiments contain an ampicillin resistance gene (ampR) is 
    • A. 

      So that the plasmid can replicate in bacterial cells.

    • B. 

      So that the plasmid can recombine with the bacterial genome. 

    • C. 

      To enable the recipient cell to conjugate

    • D. 

       to provide a selectable marker

    • E. 

      To map point mutations. 

  • 11. 
    In a genetic cross between two bacteriophage T4 rII mutants, r+ recombinants are produced. The genetic changes in these two rII mutants must be 
    • A. 

      Heterozygous

    • B. 

      Heteroallelic

    • C. 

      Homozygous.

    • D. 

      Homoallelic. 

    • E. 

       polymorphic. 

  • 12. 
    In E. coli, λ DNA integrates into which site on the bacterial chromosome? 
    • A. 

      Ori

    • B. 

      Trp

    • C. 

      Bio

    • D. 

      Gal

    • E. 

      Att

  • 13. 
    To grow on artificial medium, prototrophic bacteria require 
    • A. 

      Sunlight. 

    • B. 

       amino acid supplements

    • C. 

       vitamin supplements

    • D. 

      All of these

    • E. 

       None of these 

  • 14. 
    E. coli strains that are Hfr 
    • A. 

      Are easily mutated

    • B. 

      Are susceptible to infection by bacteriophage

    • C. 

      Contain the F factor integrated in the bacterial chromosome. 

    • D. 

       have a low frequency of recombination

  • 15. 
     The E. coli chromosome is 
    • A. 

      Linear. 

    • B. 

       circular. 

    • C. 

      Single‐stranded. 

    • D. 

      Less than a megabase in length. 

    • E. 

       compartmentalized within an intracellular membrane

  • 16. 
    Which of the following methods can be used to create competent bacterial cells? 
    • A. 

       Treating the cells chemically

    • B. 

       Exposing cells to a strong electric field 

    • C. 

       Making the cell membrane more permeable to DNA

    • D. 

       Allowing the culture to enter stationary phase growth

    • E. 

       A, B, and C only 

  • 17. 
    In general, the transformation of most genes occurs at a frequency of one in every ________ cells. 
    • A. 

      10

    • B. 

      100

    • C. 

      1,000

    • D. 

      10,000

    • E. 

      1,000,000 

  • 18. 
    Which of the following statements is true regarding a temperate phage? 
    • A. 

       It can reproduce at warm or cool temperatures

    • B. 

       It is required for transformation

    • C. 

      It can be lytic or lysogenic

    • D. 

       It causes bacterial cells to conjugate

    • E. 

       It reproduces by binary fission

  • 19. 
    In the lysogenic bacteriophage life cycle, the λ chromosome.
    • A. 

      Replicates and the phage genes take over the bacterium

    • B. 

       inserts itself physically into the host cellʹs chromosome

    • C. 

      Expresses a repressor protein gene that inhibits the lytic pathway. 

    • D. 

       is replaced by a piece of bacterial DNA when packaged inside phage progeny. 

    • E. 

      Both B and C 

  • 20. 
    Co Transduction bacteria can occur if 
    • A. 

      A bacterial cell picks up two fragments of DNA from the environment, each with a different gene

    • B. 

       double crossover occurs between prophage DNA and the bacterial chromosome.

    • C. 

      Two genes are closely linked enough so that they can be packaged into a phage head and injected into a cell by a single phage

    • D. 

       two genes are introduced into the same bacterium by simultaneous infection with two different phages

    • E. 

       Both C and D 

  • 21. 
    Genetic exchange in bacteria by transduction requires cell‐to‐cell contact. 
    • A. 

      True

    • B. 

      False

  • 22. 
    The viral chromosome of a bacteriophage can never integrate into the host bacteriumʹs chromosome. 
    • A. 

      True

    • B. 

      False

  • 23. 
    Genetic mapping experiments performed by Seymour Benzer on rII mutants in the T4 phage showed that the gene is indivisible by the process of mutation and recombination.
    • A. 

      True

    • B. 

      False

  • 24. 
    In the case of genetic exchange in bacteria by transformation, there is an exchange of DNA back and forth between cells, and a complete diploid cell is formed. 
    • A. 

      True

    • B. 

      False

  • 25. 
    In genetic exchange by conjugation, a single Hfrstrain will transfer the entire bacterial chromosome to the recipient, allowing mapping in minutes from the first gene entering to the last one before the other half of the inserted F factor. 
    • A. 

      True

    • B. 

      False