The net gain of ATP once the lactic acid system is completed is 2. This system is an anaerobic pathway that occurs when there is a lack of oxygen available for energy production. During this process, glucose is broken down into pyruvate, which is then converted into lactic acid. In this process, a total of 4 ATP molecules are produced, but 2 ATP molecules are used in the initial steps, resulting in a net gain of 2 ATP molecules.