R-DNA Technology Quiz Questions & Answers
Welcome to the "R-DNA Technology" quiz! This exciting quiz is designed to assess your knowledge and understanding of the principles, techniques, and applications of R-DNA technology.
The quiz will also test your knowledge of the essential components used in R-DNA technology, such as plasmids and vectors. You will discover why plasmids are employed as carriers for introducing foreign DNA into host organisms and the significance of selecting appropriate host organisms for successful genetic manipulation.
Furthermore, you will encounter questions about the techniques utilized for DNA amplification, sequencing, and analysis. You will have the opportunity to showcase your understanding of polymerase chain reaction (PCR), Read morea powerful method for exponentially amplifying specific DNA sequences. Additionally, you will explore the potential applications of R-DNA technology, ranging from the production of recombinant proteins to gene therapy and genetic modification of crops.
By taking this quiz, you will not only test your knowledge of R-DNA technology but also deepen your understanding of its significance in various fields. So, put your thinking cap on and get ready to unravel the fascinating world of R-DNA technology. Good luck, and enjoy the quiz!
- 1.
What does R-DNA stand for in R-DNA Technology?
- A.
Ribonucleic Acid-DNA
- B.
Recombinant DNA
- C.
Replication DNA
- D.
Retroviral DNA
Correct Answer
B. Recombinant DNA -
- 2.
Which enzyme is commonly used in R-DNA technology to cut DNA at specific sites?
- A.
Restriction endonucleases
- B.
DNA polymerase I
- C.
Reverse transcriptase
- D.
DNA ligase
Correct Answer
A. Restriction endonucleases -
- 3.
Which organism is often used as a host for recombinant DNA technology?
- A.
Saccharomyces cerevisiae
- B.
Drosophila melanogaster
- C.
Arabidopsis thaliana
- D.
Escherichia coli
Correct Answer
D. Escherichia coli -
- 4.
What is the function of DNA ligase in R-DNA technology?
- A.
Cutting DNA at specific sites
- B.
Amplifying DNA
- C.
Joining DNA fragments
- D.
Transcribing RNA
Correct Answer
C. Joining DNA fragments -
- 5.
What is a vector in the context of R-DNA technology?
- A.
Organism used to produce vaccines
- B.
Enzyme used to replicate DNA
- C.
DNA molecule used to carry foreign DNA
- D.
Segment of RNA used in protein synthesis
Correct Answer
C. DNA molecule used to carry foreign DNA -
- 6.
What is the purpose of the selectable marker in a plasmid vector?
- A.
Identifying cells that have taken up the recombinant DNA
- B.
Amplifying the target gene
- C.
Inactivating the host cell's DNA repair system
- D.
Producing fluorescent proteins
Correct Answer
A. Identifying cells that have taken up the recombinant DNA -
- 7.
What is the role of reverse transcriptase in R-DNA technology?
- A.
Amplifying DNA using PCR
- B.
Cutting DNA at specific sites
- C.
Synthesizing DNA from RNA templates
- D.
Joining DNA fragments
Correct Answer
C. Synthesizing DNA from RNA templates -
- 8.
Which technique is used to amplify DNA sequences in the laboratory?
- A.
Polymerase Chain Reaction (PCR)
- B.
Gel Electrophoresis
- C.
DNA Sequencing
- D.
Western Blot
Correct Answer
A. Polymerase Chain Reaction (PCR) -
- 9.
What is the purpose of gel electrophoresis in R-DNA technology?
- A.
Separating DNA fragments by size
- B.
Cloning genes
- C.
Inserting foreign DNA into host cells
- D.
Identifying protein interactions
Correct Answer
A. Separating DNA fragments by size -
- 10.
What is the name of the process used to create multiple copies of a specific DNA sequence?
- A.
DNA transcription
- B.
DNA translation
- C.
DNA replication
- D.
DNA amplification
Correct Answer
D. DNA amplification -
- 11.
Which ethical concern is associated with R-DNA technology?
- A.
Genetic manipulation and human cloning
- B.
Environmental pollution
- C.
Intellectual property rights
- D.
Technological unemployment
Correct Answer
A. Genetic manipulation and human cloning -
- 12.
What is the purpose of rDNA technology?
- A.
Treating diseases
- B.
Creating genetically modified organisms
- C.
Producing renewable energy
- D.
Designing new materials
Correct Answer
B. Creating genetically modified organisms -
- 13.
Which enzyme is commonly used to cut DNA at specific sites in rDNA technology?
- A.
DNA polymerase
- B.
RNA polymerase
- C.
Ligase
- D.
Restriction enzyme
Correct Answer
D. Restriction enzymeExplanation
In recombinant DNA (rDNA) technology, the enzyme used to cut DNA at specific sites is a Restriction enzyme. Restriction enzymes, also known as restriction endonucleases, recognize specific, short nucleotide sequences within DNA molecules and cleave the DNA at or near these sites. This ability makes them essential tools for genetic engineering, allowing scientists to cut DNA from different sources in a precise manner to construct recombinant DNA molecules.Rate this question:
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- 14.
What is a plasmid in the context of rDNA technology?
- A.
A type of animal cell
- B.
A type of virus
- C.
A small, circular DNA molecule
- D.
A type of bacterial cell
Correct Answer
C. A small, circular DNA molecule -
- 15.
What is the purpose of a selectable marker in rDNA technology?
- A.
To visualize DNA under a microscope
- B.
To insert foreign DNA into a host cell
- C.
To track the movement of DNA
- D.
To identify cells that have taken up the recombinant DNA
Correct Answer
D. To identify cells that have taken up the recombinant DNA -
- 16.
Which of the following is an application of rDNA technology in medicine?
- A.
Gene therapy
- B.
Food preservation
- C.
Renewable energy production
- D.
Water purification
Correct Answer
A. Gene therapy -
- 17.
What is the role of a vector in rDNA technology?
- A.
To carry the recombinant DNA into host cells
- B.
To amplify the DNA in a laboratory setting
- C.
To cut the DNA into smaller fragments
- D.
To analyze the DNA sequence
Correct Answer
A. To carry the recombinant DNA into host cells -
- 18.
Which organism is commonly used as a host for the production of recombinant proteins?
- A.
Bacteria
- B.
Plants
- C.
Animals
- D.
Fungi
Correct Answer
A. Bacteria -
- 19.
What is the purpose of DNA sequencing in R-DNA technology?
- A.
To analyze the order of nucleotides in a DNA molecule
- B.
To separate DNA fragments by size
- C.
To amplify specific DNA sequence
- D.
To insert foreign DNA into a host cell
Correct Answer
A. To analyze the order of nucleotides in a DNA molecule -
- 20.
What is the purpose of using a selectable marker gene in R-DNA Technology?
- A.
To enhance the expression of the target gene
- B.
To insert foreign DNA into the host genome
- C.
To identify transformed cells
- D.
To increase the efficiency of DNA replication
Correct Answer
C. To identify transformed cells -
Quiz Review Timeline +
Our quizzes are rigorously reviewed, monitored and continuously updated by our expert board to maintain accuracy, relevance, and timeliness.
-
Current Version
-
Apr 15, 2024Quiz Edited by
ProProfs Editorial Team -
Jul 13, 2023Quiz Created by
Amit Mangal
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