Lesson Overview
When students can't explain why enzymes fail at high temperatures or how proteins are built, it shows a gap in understanding the fundamentals of biochemistry. This lesson simplifies complex terms-like amino acids, bonding, and pH-so you can confidently grasp the chemical principles behind how cells function and life is sustained.
What Is Biochemistry and Why Is It Crucial?
Biochemistry is the branch of science that explores the chemical substances and processes that occur in living organisms. It focuses on the composition, structure, and transformation of molecules such as:
- Proteins
- Lipids
- Carbohydrates
- Nucleic acids
Biochemistry explains how these molecules interact, how they store and release energy, and how they are used in cellular functions like growth, repair, reproduction, and metabolism.
The Four Major Biomolecules: Structure and Function
Living organisms rely on macromolecules-large, complex compounds formed from smaller building blocks (monomers) through chemical reactions. These biomolecules include:
A. Carbohydrates
- Elements: Carbon (C), Hydrogen (H), Oxygen (O)
- General formula: CH₂O
- Functions:
- Primary energy source for cells
- Structural support (e.g., cellulose in plant cell walls)
Types of Carbohydrates:
- Monosaccharides (simple sugars): Glucose, fructose
- Disaccharides: Sucrose (glucose + fructose), lactose
- Polysaccharides: Starch (plant energy storage), glycogen (animal energy storage), cellulose (plant structure)
B. Proteins
- Monomers: Amino acids (20 types)
- Structure:
- Central carbon
- Amino group (-NH₂)
- Carboxyl group (-COOH)
- Variable R group (defines the type)
Peptide bonds form between amino acids during dehydration synthesis, creating long chains called polypeptides. These fold into complex 3D structures which determine function.
Functions of Proteins:
- Enzymes (e.g., amylase)
- Hormones (e.g., insulin)
- Antibodies
- Structural components (e.g., collagen)
- Transport (e.g., hemoglobin)
C. Lipids
- Components: Glycerol + 3 fatty acids (triglycerides)
- Types: Fats, oils, waxes, phospholipids, steroids
Saturated fats have no double bonds (solid at room temperature, e.g., butter).
Unsaturated fats contain one or more double bonds (liquid at room temperature, e.g., olive oil).
Functions:
- Long-term energy storage
- Insulation and protection
- Component of cell membranes (phospholipids)
- Hormone synthesis (e.g., steroids like testosterone)
D. Nucleic Acids (DNA & RNA)
- Monomers: Nucleotides
- Each nucleotide includes:
- Phosphate group
- 5-carbon sugar (ribose or deoxyribose)
- Nitrogenous base (A, T/U, G, C)
Functions:
- DNA stores genetic information
- RNA helps make proteins from DNA instructions
Bonding and Atomic Structure in Biochemistry
Atomic Components:
- Protons (+) in nucleus
- Neutrons (neutral) in nucleus
- Electrons (-) orbit nucleus
Important Bond Types:
- Covalent bonds: Shared electrons (strong)
- Ionic bonds: Transferred electrons (between oppositely charged ions)
- Polar covalent bonds: Unequal electron sharing (e.g., in water)
- Hydrogen bonds: Weak attraction between partially charged atoms (e.g., between water molecules or DNA strands)
Isotopes:
Atoms with the same number of protons but different numbers of neutrons.
Example: Carbon-12 vs. Carbon-14.
Isotopes help track molecules in metabolic studies using radioactive labeling (e.g., tracking nitrogen in protein synthesis).
Water: The Universal Solvent
Water's unique polarity makes it essential to life.
Key Properties of Water:
- Polarity: The oxygen end is slightly negative; hydrogen ends are slightly positive.
- Hydrogen bonding: Holds water molecules together.
- Cohesion: Water sticks to itself (important for surface tension).
- Adhesion: Water sticks to other surfaces (important for capillary action).
- High specific heat: Resists temperature changes-important for body temperature regulation.
- Ice floats: Because solid water is less dense than liquid-protects aquatic life in winter.
These properties support:
- Nutrient transport
- Waste removal
- Temperature regulation
- Chemical reactions in cells
Acids, Bases, and the pH Scale
pH = Potential of Hydrogen
- Scale: 0 (most acidic) → 14 (most basic)
- Acid: Releases H⁺ ions (e.g., H₂SO₄)
- Base: Releases OH⁻ ions or absorbs H⁺
- Neutral: pH 7 (e.g., pure water)
Enzyme activity is pH-dependent. Most enzymes in the human body function best at a pH of 6–8.
Enzymes: Catalysts of Life
Enzymes are proteins that accelerate chemical reactions by lowering activation energy.
How Enzymes Work:
- The substrate binds to the enzyme's active site (lock-and-key model).
- The enzyme modifies the substrate.
- The product is released; the enzyme is unchanged.
Enzyme Examples:
- Amylase breaks down starch
- Lactase breaks down lactose
- DNA polymerase builds DNA strands
Factors Affecting Enzymes:
- Temperature (too high = denaturation)
- pH (each enzyme has an optimal pH)
- Substrate concentration
- Presence of inhibitors
Macromolecule Synthesis and Breakdown
A. Dehydration Synthesis (Condensation)
- Monomers combine to form polymers
- Water is removed during bond formation
- Example: Amino acids → protein (via peptide bonds)
B. Hydrolysis
- Polymers are broken into monomers
- Water is added to break the bonds
- Example: Starch → glucose
These reactions are mediated by enzymes and occur constantly during digestion and biosynthesis.
Identifying Biomolecules by Structure and Role
Quick ID Tips:
- Amino acids: Have an amine group (-NH₂), carboxyl group (-COOH), central carbon
- Nucleotides: Phosphate + sugar + nitrogenous base
- Simple sugars: Carbon ring with multiple -OH groups
- Fatty acids: Long hydrocarbon chain + carboxyl group
Each structure determines the molecule's role, reactivity, and interaction with other biomolecules.
Functional Groups in Biochemistry
Functional groups give molecules their chemical characteristics. Key groups include:
- Hydroxyl (-OH): In sugars, increases solubility
- Carboxyl (-COOH): In fatty and amino acids, acidic properties
- Amino (-NH₂): Found in amino acids, makes molecules basic
- Phosphate (-PO₄): Found in ATP and DNA; involved in energy transfer
Understanding these helps in predicting reaction behavior and molecule classification.
Scientists use radioactive isotopes, such as radioactive nitrogen, to trace how proteins are built and metabolized in cells. Since nitrogen is found in amino acids but not in fats or carbohydrates, it allows precise tracking of protein synthesis in living organisms.
This is a key method in molecular biology, medicine, and biotechnology.
Conclusion
Biochemistry helps us understand life from the molecular level up. By mastering the structure and function of proteins, carbohydrates, lipids, and nucleic acids, and by exploring the properties of water, enzymes, and bonding, students gain a solid foundation in the science that drives every cell in the human body.