Quiz:What Do You Know About Genes and DNA?

1. We clone human genes to

Define inherited genetic mutations

Isolate functional genes

Isolate genes with somatic mutations

Define and understand cellular pathways and circuits

All of the above

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3. Genes are

Spread equally throughout the genome

Concentrated in random areas in the genome

Equally distributed on all chromosomes

All about the same size

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5. Bacterial expression systems:

Are smelly and expensive

Lack enzymes to catalyze post-translational modifications

Provide the only way to produce an active drug

Are easy to screen by high throughput techniques

Are used to develop vaccines

6. You can detect a single molecule of DNA using

Northern blot

Microarray

FISH

Southern blot

PCR

7. Less than _______ of the human genome encodes proteins

50%

25%

80%

5%

2%

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9. Southern blots can detect

Differences in mRNA

All point mutations

Only point mutations that alter restriction enzyme recognition sites

Small deletions

Expression of a gene in specific tissues or cells

10. Factor VIII for hemophiliacs was produced using

Bacteria

Viruses

Yeast

Mammalian cells

New biochemical techniques for synthesis of natural products

Quiz:What Do You Know About Genes and DNA?

1. We clone human genes to

2.

What first name or nickname would you like us to use?

2. G-CSF is produced using a _______________ expression system

3. Genes are

4. ____________ is used to clone cDNA and assess level of gene expression.

5. Bacterial expression systems:

6. You can detect a single molecule of DNA using

7. Less than _______ of the human genome encodes proteins

8. An __________________ is a modified plasmid or virus carrying a gene or cDNA into a suitable host cell where it directs expression of the encoded protein

9. Southern blots can detect

10. Factor VIII for hemophiliacs was produced using