The Transgenic Fly Virtual Lab: What Do You Know? Trivia Facts Quiz

15 Questions | Total Attempts: 372

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The Transgenic Fly Virtual Lab: What Do You Know? Trivia Facts Quiz - Quiz

The trivia facts quiz below is designed to test out what you know about the Transgenic Fly Virtual Lab. If you want to learn about DNA the best way to do so is in these labs. Do you know the rules you should be following while you are at the lab? Do take up this quiz and see if you know the lab that well.


Questions and Answers
  • 1. 
    In this experiment, the reporter gene
    • A. 

      Tells the experimenter exactly how much of the native protein was produced

    • B. 

      Changes the color of the flies' eyes

    • C. 

      Must be from the same type of organism as the organism into which it is being transferred

    • D. 

      Is designed to be easily detected and reflect the activity of the native gene

  • 2. 
    After successfully incorporating into the genome of a fly, the construct DNA will
    • A. 

      Change the functions of the native pergene

    • B. 

      Cause the eyes to be white

    • C. 

      Reflect transcriptional activity without interfering with functions

    • D. 

      Alter the biological clock

  • 3. 
     Both fireflies and per-Luc transgenic Drosophila
    • A. 

      Can be seen glowing outdoors at dusk

    • B. 

      Produce the enzyme luciferase

    • C. 

      Use their glow to attract mates

    • D. 

      Are experimentally produced

  • 4. 
    Which of the following DNA sequences is not found in the "construct DNA?"
    • A. 

      Transposase

    • B. 

      Luciferase

    • C. 

      Period gene promoter

    • D. 

      P element

  • 5. 
     Why is it important to monitor the age of the fly embryos?
    • A. 

      Cell membranes form around each nucleus in the multi-nucleated embryonic cell mass at approximately 30—60 minutes.

    • B. 

      Germ cells can incorporate DNA before differentiation, which begins when the embryo is approximately 30—60 minutes old.

    • C. 

      Embryos older than 30—60 minutes will not incorporate the DNA and will dilute the number of successfully injected flies

    • D. 

      All of the above.

  • 6. 
    An experimentally useful per-luctransgenic fly is produced
    • A. 

      When the injected fly embryo becomes an adult

    • B. 

      In all the offspring of an injected embryo that has grown to adulthood

    • C. 

      In only a few of the offspring of an injected embryo containing germ cells that have incorporated the injected DNA

    • D. 

      None of the above.

  • 7. 
     Injected fly embryos fail to survive for all of the reasons below except​​​​​​​
    • A. 

      fluid loss due to wound at injection site

    • B. 

      injection of too much fluid, causing embryo to explode

    • C. 

      embryo is too young to tolerate injection

    • D. 

      Embryo becomes too dry

  • 8. 
    When injecting flies with the construct DNA, which of the following steps will help increase the chances that transgenic progeny are produced?
    • A. 

      Inject a large volume of DNA solution.

    • B. 

      Inject many embryos.

    • C. 

      Inject into the outermost embryonic membrane.

    • D. 

      Destroy all older embryos

  • 9. 
    After completing a transgenic injection experiment, only white-eyed progeny result. Which of the following situations could explain the result?
    • A. 

      You did not inject enough embryos.

    • B. 

      You accidentally broke the glass needle and had to replace it with a new one in the micromanipulator. In the meantime, the embryos continued to develop, and all were more than 60 minutes old when you began the injections.

    • C. 

      All your injections missed the germ cells in the posterior region of the embryos

    • D. 

      All the above.

  • 10. 
     All the injected embryos that have grown to adulthood have white eyes. They are mated with wild-type flies that also have white eyes. How is it possible that some progeny will have red-eyes?
    • A. 

      Red-eyed flies contaminate the experiment.

    • B. 

      Progeny with red-eyes are the result of matings in which the germ cell from one parent contains the transgene

    • C. 

      A mutation occurs that produces red eyes

    • D. 

      Progeny with red eyes occur in flies raised in constant darkness

  • 11. 
    How is light produced by the transgenic flies?
    • A. 

      The same way that light is produced in fireflies.

    • B. 

      The transgenic flies have eaten a substance that produces light

    • C. 

      Transcription of the transgene results in production of luciferase, which acts on luciferin.

    • D. 

      Turning on the room lights causes light to be produced by the flies.

  • 12. 
    2. Maximal light output from the transgenic fly occurs when the promoter of which gene is activated?
    • A. 

      Period

    • B. 

      Luciferase

    • C. 

      Mini-white

    • D. 

      Timeless

  • 13. 
     Can external light cues reset the flies' clock
    • A. 

      NO

    • B. 

      YES

    • C. 

      Option 3

    • D. 

      Option 4

  • 14. 
    Measurements of per-luc gene expression from an individual fly wing
    • A. 

      Have more variability than that observed from data averaged from 20 flies

    • B. 

      Show similar trends compared with data from whole flies

    • C. 

      Dampen over time

    • D. 

      All the above.

  • 15. 
    When the altered day-night schedule (similar to that used in Experiment 2) was put in place on days 8, 9, and 10
    • A. 

      Oscillations in per-luc activity were no longer observed

    • B. 

      Peak oscillations occurred at about the same time of day as was observed on days 1, 2, and 3

    • C. 

      The trough was observed at the beginning of daytime

    • D. 

      The oscillations in the fly wing reset to the new schedule (days 8,9,10), which was 6 hours later than the original schedule (days 1, 2, 3)

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