The Transgenic Fly Virtual Lab: What Do You Know? Trivia Facts Quiz

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| By Chris Jamali
Chris Jamali, Software
Chris, a skilled Software Engineer, leverages expertise to design and develop cutting-edge software solutions, contributing to technological advancements and innovation.
Quizzes Created: 1 | Total Attempts: 523
| Attempts: 523
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  • 1/15 Questions

     Can external light cues reset the flies' clock

    • NO
    • YES
    • Option 3
    • Option 4
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About This Quiz

The trivia facts quiz below is designed to test out what you know about the Transgenic Fly Virtual Lab. If you want to learn about DNA the best way to do so is in these labs. Do you know the rules you should be following while you are at the lab? Do take up this quiz and see if you know the lab that well.

The Transgenic Fly Virtual Lab: What Do You Know? Trivia Facts Quiz - Quiz

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  • 2. 

    After successfully incorporating into the genome of a fly, the construct DNA will

    • Change the functions of the native pergene

    • Cause the eyes to be white

    • Reflect transcriptional activity without interfering with functions

    • Alter the biological clock

    Correct Answer
    A. Reflect transcriptional activity without interfering with functions
    Explanation
    The construct DNA, after being incorporated into the genome of a fly, will reflect transcriptional activity without interfering with functions. This means that the construct DNA will be able to accurately indicate the level of gene expression without disrupting the normal functioning of the native genes. It will allow researchers to study gene activity without causing any unwanted alterations or disturbances in the biological processes of the fly.

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  • 3. 

    Which of the following DNA sequences is not found in the "construct DNA?"

    • Transposase

    • Luciferase

    • Period gene promoter

    • P element

    Correct Answer
    A. Transposase
    Explanation
    The DNA sequence "transposase" is not found in the "construct DNA".

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  • 4. 

     Both fireflies and per-Luc transgenic Drosophila

    • Can be seen glowing outdoors at dusk

    • Produce the enzyme luciferase

    • Use their glow to attract mates

    • Are experimentally produced

    Correct Answer
    A. Produce the enzyme luciferase
    Explanation
    Both fireflies and per-Luc transgenic Drosophila produce the enzyme luciferase. Luciferase is responsible for the bioluminescent glow exhibited by these organisms. This enzyme catalyzes a reaction that produces light, allowing fireflies and per-Luc transgenic Drosophila to emit their characteristic glow. This glow is used by both species to attract mates and communicate with each other. The production of luciferase is a natural process in fireflies, while in per-Luc transgenic Drosophila, it is artificially introduced through genetic modification.

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  • 5. 

    When the altered day-night schedule (similar to that used in Experiment 2) was put in place on days 8, 9, and 10

    • Oscillations in per-luc activity were no longer observed

    • Peak oscillations occurred at about the same time of day as was observed on days 1, 2, and 3

    • The trough was observed at the beginning of daytime

    • The oscillations in the fly wing reset to the new schedule (days 8,9,10), which was 6 hours later than the original schedule (days 1, 2, 3)

    Correct Answer
    A. The oscillations in the fly wing reset to the new schedule (days 8,9,10), which was 6 hours later than the original schedule (days 1, 2, 3)
    Explanation
    The correct answer is that the oscillations in the fly wing reset to the new schedule (days 8,9,10), which was 6 hours later than the original schedule (days 1, 2, 3). This means that the fly's biological rhythm adjusted to the altered day-night schedule and shifted its peak oscillations to occur 6 hours later than before. This indicates that the fly's internal clock is flexible and can adapt to changes in the environment.

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  • 6. 

     All the injected embryos that have grown to adulthood have white eyes. They are mated with wild-type flies that also have white eyes. How is it possible that some progeny will have red-eyes?

    • Red-eyed flies contaminate the experiment.

    • Progeny with red-eyes are the result of matings in which the germ cell from one parent contains the transgene

    • A mutation occurs that produces red eyes

    • Progeny with red eyes occur in flies raised in constant darkness

    Correct Answer
    A. Progeny with red-eyes are the result of matings in which the germ cell from one parent contains the transgene
    Explanation
    The correct answer is that progeny with red-eyes are the result of matings in which the germ cell from one parent contains the transgene. This means that one of the parents carries a gene that causes red eyes, which is passed on to some of the offspring. It is not mentioned that red-eyed flies contaminate the experiment, a mutation occurs, or that red-eyed progeny occur in flies raised in constant darkness. Therefore, the only plausible explanation is the presence of the transgene in one of the parents.

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  • 7. 

    How is light produced by the transgenic flies?

    • The same way that light is produced in fireflies.

    • The transgenic flies have eaten a substance that produces light

    • Transcription of the transgene results in production of luciferase, which acts on luciferin.

    • Turning on the room lights causes light to be produced by the flies.

    Correct Answer
    A. Transcription of the transgene results in production of luciferase, which acts on luciferin.
    Explanation
    The correct answer explains that light is produced in transgenic flies through the process of transcription of the transgene. This transgene produces luciferase, which then acts on luciferin to generate light. This process is similar to how light is produced in fireflies.

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  • 8. 

    After completing a transgenic injection experiment, only white-eyed progeny result. Which of the following situations could explain the result?

    • You did not inject enough embryos.

    • You accidentally broke the glass needle and had to replace it with a new one in the micromanipulator. In the meantime, the embryos continued to develop, and all were more than 60 minutes old when you began the injections.

    • All your injections missed the germ cells in the posterior region of the embryos

    • All the above.

    Correct Answer
    A. All the above.
    Explanation
    All the above situations could explain the result of only white-eyed progeny after completing a transgenic injection experiment. If the experimenter did not inject enough embryos, it is possible that only white-eyed progeny were observed due to a limited sample size. If the glass needle was accidentally broken and replaced with a new one while the embryos continued to develop, the delay in injections could have affected the outcome, resulting in white-eyed progeny. Additionally, if all the injections missed the germ cells in the posterior region of the embryos, it could lead to the observed result of only white-eyed progeny.

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  • 9. 

    When injecting flies with the construct DNA, which of the following steps will help increase the chances that transgenic progeny are produced?

    • Inject a large volume of DNA solution.

    • Inject many embryos.

    • Inject into the outermost embryonic membrane.

    • Destroy all older embryos

    Correct Answer
    A. Inject many embryos.
    Explanation
    Injecting many embryos increases the chances of producing transgenic progeny because it increases the number of embryos that have the potential to successfully incorporate the construct DNA. By injecting a larger number of embryos, there is a higher probability that at least some of them will successfully integrate the DNA into their genome and develop into transgenic individuals. This increases the overall success rate of producing transgenic progeny.

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  • 10. 

    An experimentally useful per-luctransgenic fly is produced

    • When the injected fly embryo becomes an adult

    • In all the offspring of an injected embryo that has grown to adulthood

    • In only a few of the offspring of an injected embryo containing germ cells that have incorporated the injected DNA

    • None of the above.

    Correct Answer
    A. In only a few of the offspring of an injected embryo containing germ cells that have incorporated the injected DNA
    Explanation
    When a fly embryo is injected with DNA and grows to adulthood, the injected DNA can be incorporated into the germ cells of the fly. This means that only the offspring that inherit these germ cells will have the injected DNA. Therefore, the experimentally useful per-luctransgenic fly is produced in only a few of the offspring of an injected embryo containing germ cells that have incorporated the injected DNA.

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  • 11. 

    Measurements of per-luc gene expression from an individual fly wing

    • Have more variability than that observed from data averaged from 20 flies

    • Show similar trends compared with data from whole flies

    • Dampen over time

    • All the above.

    Correct Answer
    A. All the above.
    Explanation
    The given correct answer is "All the above." This means that all of the statements provided in the question are true. Measurements of per-luc gene expression from an individual fly wing have more variability than that observed from data averaged from 20 flies, they show similar trends compared with data from whole flies, and they dampen over time.

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  • 12. 

    In this experiment, the reporter gene

    • Tells the experimenter exactly how much of the native protein was produced

    • Changes the color of the flies' eyes

    • Must be from the same type of organism as the organism into which it is being transferred

    • Is designed to be easily detected and reflect the activity of the native gene

    Correct Answer
    A. Is designed to be easily detected and reflect the activity of the native gene
    Explanation
    The reporter gene is designed to be easily detected and reflect the activity of the native gene. This means that when the native gene is active and producing its protein, the reporter gene will also be active and produce a detectable signal. This allows the experimenter to easily determine the level of activity of the native gene by measuring the activity of the reporter gene. It is important for the reporter gene to be easily detected so that the experimenter can accurately measure the activity of the native gene.

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  • 13. 

     Injected fly embryos fail to survive for all of the reasons below except​​​​​​​

    • fluid loss due to wound at injection site

    • injection of too much fluid, causing embryo to explode

    • embryo is too young to tolerate injection

    • Embryo becomes too dry

    Correct Answer
    A. embryo is too young to tolerate injection
    Explanation
    The correct answer is "embryo is too young to tolerate injection". This is because the other options listed - fluid loss due to wound at the injection site, injection of too much fluid causing the embryo to explode, and embryo becoming too dry - all provide valid reasons for why injected fly embryos fail to survive. However, the age of the embryo does not determine its ability to tolerate injection.

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  • 14. 

     Why is it important to monitor the age of the fly embryos?

    • Cell membranes form around each nucleus in the multi-nucleated embryonic cell mass at approximately 30—60 minutes.

    • Germ cells can incorporate DNA before differentiation, which begins when the embryo is approximately 30—60 minutes old.

    • Embryos older than 30—60 minutes will not incorporate the DNA and will dilute the number of successfully injected flies

    • All of the above.

    Correct Answer
    A. All of the above.
    Explanation
    It is important to monitor the age of the fly embryos because cell membranes start forming around each nucleus in the multi-nucleated embryonic cell mass at approximately 30-60 minutes. Additionally, germ cells can incorporate DNA before differentiation, which begins when the embryo is approximately 30-60 minutes old. Embryos older than 30-60 minutes will not incorporate the DNA and will dilute the number of successfully injected flies. Therefore, monitoring the age of the fly embryos is crucial to ensure successful DNA incorporation and avoid wasting resources on embryos that are too old.

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  • 15. 

    2. Maximal light output from the transgenic fly occurs when the promoter of which gene is activated?

    • Period

    • Luciferase

    • Mini-white

    • Timeless

    Correct Answer
    A. Period
    Explanation
    The maximal light output from the transgenic fly occurs when the promoter of the "period" gene is activated. This suggests that the "period" gene plays a crucial role in regulating the production of light in the transgenic fly. The activation of its promoter leads to an increase in the expression of luciferase, which is responsible for the light production. This finding highlights the importance of the "period" gene in the biological processes underlying light output in the transgenic fly.

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  • Current Version
  • Mar 21, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • Mar 25, 2019
    Quiz Created by
    Chris Jamali
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