Techniques In Plant Analysis

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| By Ashish Sharma
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Ashish Sharma
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Quizzes Created: 1 | Total Attempts: 111
| Attempts: 111
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  • 1/10 Questions

    What does a spectrophotometer directly measure?

    • The amount of a chemical in a material
    • The amount of light that a substance absorbs
    • What wavelength of light we are seeing
    • The weight of a material
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About This Quiz

This quiz covers key techniques in plant analysis using spectroscopy, focusing on instrument components and their functions.

Techniques In Plant Analysis - Quiz

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  • 2. 

    In a spectrophotometer, the detector changes the light signal into______

    • Current

    • Voltage

    • Resistance

    • None of these

    Correct Answer
    A. Current
    Explanation
    In a spectrophotometer, the detector is responsible for converting the light signal into an electrical signal. This electrical signal is typically in the form of current, which can be measured and analyzed to determine the intensity or wavelength of the light being detected. Therefore, the correct answer is current.

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  • 3. 

    In a spectrophotometer, the function of prism is to________

    • Change light path

    • Focus the light

    • Converge the light

    • Disperse the light

    Correct Answer
    A. Disperse the light
    Explanation
    The function of a prism in a spectrophotometer is to disperse the light. When light passes through a prism, it is separated into its component colors, creating a spectrum. This dispersion allows the spectrophotometer to analyze the different wavelengths of light and measure their intensity, which is essential for various applications such as determining the concentration of a substance in a solution.

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  • 4. 

    Grating is a device used in the spectrophotometer to function as________

    • A light source

    • A nebulizer

    • A monochromator

    • A detector

    Correct Answer
    A. A monochromator
    Explanation
    A spectrophotometer is a device used to measure the intensity of light at different wavelengths. A monochromator is a crucial component of a spectrophotometer as it allows the selection of a specific wavelength of light. It works by dispersing white light into its individual colors and then selecting a narrow band of wavelengths using a prism or a diffraction grating. This selected wavelength is then passed through the sample being analyzed. Therefore, a monochromator is used in a spectrophotometer to control and select the wavelength of light that is used for measurement.

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  • 5. 

    In the spectrophotometers used conventionally the width of the cuvette is usually fixed at_____

    • > 1 cm

    • < 1 cm

    • = 1 cm

    • Between 0 and 1 cm

    Correct Answer
    A. = 1 cm
    Explanation
    The correct answer is = 1 cm. In conventional spectrophotometers, the width of the cuvette is typically fixed at 1 cm. This is because a standard cuvette has a width of 1 cm, allowing for consistent and accurate measurements. The fixed width ensures that the light passing through the sample has a consistent path length, which is essential for accurate absorption measurements.

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  • 6. 

    Which of the following parts of the spectorphotometer is responsible for generating unilateral light of single wavelength?

    • Light source

    • Monochromator

    • Sample Chamber

    • Both a and b

    Correct Answer
    A. Monochromator
    Explanation
    The monochromator in a spectrophotometer is responsible for generating unilateral light of single wavelength. It achieves this by using a prism or diffraction grating to separate white light into its individual wavelengths and then selecting a specific wavelength to be transmitted through the sample. The monochromator ensures that only light of the desired wavelength reaches the sample, allowing for accurate measurement of absorbance or transmittance. The light source, on the other hand, provides the initial light for the spectrophotometer but does not generate unilateral light of single wavelength.

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  • 7. 

    The wavelength range between 400-700 nm can only be used to measure colored solutions.

    • True

    • False

    Correct Answer
    A. True
    Explanation
    The wavelength range between 400-700 nm corresponds to the visible light spectrum. This range is commonly used to measure the absorption or transmission of light by colored solutions. Colored solutions selectively absorb certain wavelengths of light, causing them to appear colored. By measuring the amount of light absorbed within this range, we can determine the concentration or properties of the colored solution. Therefore, it is true that the wavelength range between 400-700 nm can only be used to measure colored solutions.

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  • 8. 

    Which of the following law is NOT associated with Spectroscopy?

    • Law related to concentration

    • Law related to path length

    • Law related to reciprocity

    • None of these 

    Correct Answer
    A. Law related to reciprocity
    Explanation
    The law related to reciprocity is not associated with spectroscopy. Reciprocity refers to the principle that the intensity of light absorbed or emitted by a substance is directly proportional to the concentration of the substance and the path length that the light travels through the substance. However, this law is not specifically related to spectroscopy, which is the study of the interaction between matter and electromagnetic radiation. Spectroscopy involves the measurement and analysis of the interaction of light with matter, including the absorption, emission, and scattering of light.

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  • 9. 

    For measurements in the range of 180-300 nm, which of the following cuvettes will be used?

    • Glass

    • Quartz

    • Plastic

    • All of these

    Correct Answer
    A. Quartz
    Explanation
    Quartz cuvettes will be used for measurements in the range of 180-300 nm because quartz has a high transmittance in this wavelength range. Glass cuvettes have limited transmittance in the UV range, and plastic cuvettes have even lower transmittance. Therefore, quartz cuvettes are the most suitable choice for accurate measurements in this specific wavelength range.

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  • 10. 

    According the Beer's law for colored solution, the absorbance is directly proportional to the concentration of the solution.

    • True

    • False

    Correct Answer
    A. False
    Explanation
    Beer's law states that the absorbance of a colored solution is directly proportional to the concentration of the solute, not the solution as mentioned in the question. Therefore, the statement in the question is incorrect and the correct answer is False.

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  • Current Version
  • Mar 20, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • May 02, 2020
    Quiz Created by
    Ashish Sharma

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