Microbiology Lab Mid-term

48 Questions | Total Attempts: 432

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Microbiology Lab Mid-term

Tallahassee Community College MCB2004L Mid-term review

Questions and Answers
  • 1. 
    Hands should be washed after entering the room. (True/False)
  • 2. 
    There is no reason to be concerned with the cultures used in lab as they are all non-pathogenic. (True/False)
  • 3. 
    The lab bench should be wiped down with disinfectant at the end of class. (True/False)
  • 4. 
    The only personal things that I should take to my lab bench is my notepad, writing instrument, manual and atlas. (True/False)
  • 5. 
    Used culture material (plates, swabs, toothpicks) should be disposed of in the trash can. (True/False)
  • 6. 
    The lab coat is for the protection of the student and must be worn at all times in the lab. (True/False)
  • 7. 
    Food, eating, drinking or applying make-up is not permitted in the lab as it could be hazardous to your health. (True/False)
  • 8. 
    The wearing of open toed shoes is permitted in the microbiology lab. (True/False)
  • 9. 
    I am allowed to take my cell phone to my desk. (True/False)
  • 10. 
    It is not necessary to wash hands before leaving the room. (True/False)
  • 11. 
    You should carry the microscope with _____ hands (one under the _____ and one around the _____ ).
  • 12. 
    The eyepiece of a microscope that remagnifies the image formed by the objective lens is called the _____.
  • 13. 
    If you can focus your slide under the lower powers, but are unable to focus under the higher powers (40x and oil), one likely reason is:
    • A. 

      The condenser is not focused.

    • B. 

      The light is too bright.

    • C. 

      The ocular is dirty.

    • D. 

      All the above are wrong.

  • 14. 
    The lens that focus light through the specimen is called the _____.
  • 15. 
    Two reasons why you should clean your microscope before and after use are:
    • A. 

      To remove oil from the oil immersion lens.

    • B. 

      To clean the condenser so that the light is pure.

    • C. 

      To remove any harmful 'material' from the ocular.

    • D. 

      To remove any pathogens from the objective.

  • 16. 
    When you first get your microscope and before storage, you should _____ all parts; always store the 'scope' on the _____ power objective lens; and also, turn the _____ off when the 'scope' is not being used for observation, as well as for storage.
  • 17. 
    Acid-fast bacterial cells do not _____ because their cells have large amounts of _____ that repels the water-based dyes of the Gram stain.
  • 18. 
    A gram positive reaction gives a pink/red color. (True/False)
  • 19. 
    Bacterial specimens are very _____ as well as _____, therefore we apply stain(s) to the cells to improve _____, allowing them to become more _____ under the microscope.
  • 20. 
    One genus of human pathogenic bacteria, that is acid-fast is _____.
  • 21. 
    In our lab, used slides should be disposed of in the ______.
  • 22. 
    When working with Cultures in Petri Plates, some rules to keep in mind: a. Before incubating a plate, make sure you _____ it, also _____ it with a strip of _____; b. Incubate the plate in an _____ position, in the plastic bin designated for our class.
  • 23. 
    A _________ is a medium used to distinguish among organisms based on physical differences shown by the colony.
  • 24. 
    A ______ is a medium used to determine motility of a bacterium
  • 25. 
    A _____ is a liquid medium usually used for rapid growth or dilutions in test tubes or flasks.
  • 26. 
    A _____ is a medium with a thickener that allows growth of colonies on the surface only
  • 27. 
    A ______ is a medium that inhibits the growth of certain microorganisms and therefore favors the growth of desired microorganisms
  • 28. 
    In theory, a ______ ______ is deposited on solid nutrient medium and it begins to _____: one cell makes two, _____ make _____, four make _____ (exponential growth) and keeps growing. From these cells, a bacterial _____ is formed where the original cell (CFU) was deposited. When you transfer this colony to a new sterile _____, using _____ techniques, you obtain a _____ _____.
  • 29. 
    The size of the zone of inhibition is determined by ________.
  • 30. 
    There will be a clear area (measured as the diameter in millimeters) surrounding the antibiotic disk when the antibiotic is effective in controlling (inhibiting) or killing the bacteria tested, this area is named ________.
  • 31. 
    The Kirby-Bauer Method is also called ______. This test is a valuable tool for measuring the _____ of antimicrobials against pathogenic microorganisms. All aspects of the Kirby-Bauer Method are _____ to ensure reliable results.
  • 32. 
    The letters on the antibiotic disks represent the ______ while the numbers represent the ______.
  • 33. 
    The size of the clear zone that appears around a disk where growth has been inhibited depends upon a few "things" except:  
  • 34. 
    The Kirby-Bauer Test (Disk Diffusion Test) measures the effectiveness of agents against pathogenic microorganisms. Paper disks impregnated with specified amount of an agent (printed on the disk) are dispensed onto a plate inoculated with a bacterial lawn - these agents used are:
  • 35. 
    Control of microbes is very challenging. Microbes are everywhere and are very adaptable, therefore, this is of general concern and there's a need to overcome this challenge, a need to:
  • 36. 
    Eosin Methylene Blue (EMB) Agar is a _____ medium for some Gram _____ bacteria (inhibiting the growth of Gram _____ bacteria).
  • 37. 
    MacConkey Agar is a _____ medium for the _____ family based on the ability to ferment _____.
  • 38. 
    Eosin Methylene Blue (EMB) Agar is a _____ medium, because it allows for differentiation of lactose and non-lactose _____.
  • 39. 
    Bile salts and crystal violet make MacConkey Agar a _____ medium that inhibit the growth of Gram _____ bacteria.
  • 40. 
    A non-lactose using organism would produce colonies of what color on EMB agar?
  • 41. 
    Bacterial morphology:
  • 42. 
    Bacterial morphology:
  • 43. 
    Bacterial Morphology:
  • 44. 
    Bacterial Morphology:
  • 45. 
    Bacterial Morphology:
  • 46. 
    Bacterial Morphology:
  • 47. 
    Bacterial Morphology: