Microbiology Lab Mid-term
48 Questions
| Attempts: 540
Tallahassee Community College MCB2004L Mid-term review
Questions and Answers
- 1.
Hands should be washed after entering the room. (True/False)
Correct Answer(s)
True - 2.
There is no reason to be concerned with the cultures used in lab as they are all non-pathogenic. (True/False)
Correct Answer(s)
False - 3.
The lab bench should be wiped down with disinfectant at the end of class. (True/False)
Correct Answer(s)
True - 4.
The only personal things that I should take to my lab bench is my notepad, writing instrument, manual and atlas. (True/False)
Correct Answer(s)
True - 5.
Used culture material (plates, swabs, toothpicks) should be disposed of in the trash can. (True/False)
Correct Answer(s)
False - 6.
The lab coat is for the protection of the student and must be worn at all times in the lab. (True/False)
Correct Answer(s)
True - 7.
Food, eating, drinking or applying make-up is not permitted in the lab as it could be hazardous to your health. (True/False)
Correct Answer(s)
True - 8.
The wearing of open toed shoes is permitted in the microbiology lab. (True/False)
Correct Answer(s)
False - 9.
I am allowed to take my cell phone to my desk. (True/False)
Correct Answer(s)
False - 10.
It is not necessary to wash hands before leaving the room. (True/False)
Correct Answer(s)
False - 11.
You should carry the microscope with _____ hands (one under the _____ and one around the _____ ).
Correct Answer(s)
both, base, neck - 12.
The eyepiece of a microscope that remagnifies the image formed by the objective lens is called the _____.
Correct Answer(s)
ocular - 13.
If you can focus your slide under the lower powers, but are unable to focus under the higher powers (40x and oil), one likely reason is:
- A.
The condenser is not focused.
- B.
The light is too bright.
- C.
The ocular is dirty.
- D.
All the above are wrong.
Correct Answer
D. All the above are wrong. -
- 14.
The lens that focus light through the specimen is called the _____.
Correct Answer
condenser - 15.
Two reasons why you should clean your microscope before and after use are:
- A.
To remove oil from the oil immersion lens.
- B.
To clean the condenser so that the light is pure.
- C.
To remove any harmful 'material' from the ocular.
- D.
To remove any pathogens from the objective.
Correct Answer(s)
A. To remove oil from the oil immersion lens.
C. To remove any harmful 'material' from the ocular. -
- 16.
When you first get your microscope and before storage, you should _____ all parts; always store the 'scope' on the _____ power objective lens; and also, turn the _____ off when the 'scope' is not being used for observation, as well as for storage.
Correct Answer(s)
clean, lowest, light - 17.
Acid-fast bacterial cells do not _____ because their cells have large amounts of _____ that repels the water-based dyes of the Gram stain.
Correct Answer(s)
gram stain, waxy material - 18.
A gram positive reaction gives a pink/red color. (True/False)
Correct Answer(s)
False - 19.
Bacterial specimens are very _____ as well as _____, therefore we apply stain(s) to the cells to improve _____, allowing them to become more _____ under the microscope.
Correct Answer(s)
small, transparent, contrast, visible - 20.
One genus of human pathogenic bacteria, that is acid-fast is _____.
Correct Answer(s)
mycobacterium - 21.
In our lab, used slides should be disposed of in the ______.
Correct Answer(s)
labeled small white bucket - 22.
When working with Cultures in Petri Plates, some rules to keep in mind: a. Before incubating a plate, make sure you _____ it, also _____ it with a strip of _____; b. Incubate the plate in an _____ position, in the plastic bin designated for our class.
Correct Answer(s)
label, seal, parafilm, inverted - 23.
A _________ is a medium used to distinguish among organisms based on physical differences shown by the colony.
Correct Answer(s)
differential media - 24.
A ______ is a medium used to determine motility of a bacterium
Correct Answer(s)
semi-solid media - 25.
A _____ is a liquid medium usually used for rapid growth or dilutions in test tubes or flasks.
Correct Answer(s)
broth - 26.
A _____ is a medium with a thickener that allows growth of colonies on the surface only
Correct Answer(s)
solid media - 27.
A ______ is a medium that inhibits the growth of certain microorganisms and therefore favors the growth of desired microorganisms
Correct Answer(s)
selective media - 28.
In theory, a ______ ______ is deposited on solid nutrient medium and it begins to _____: one cell makes two, _____ make _____, four make _____ (exponential growth) and keeps growing. From these cells, a bacterial _____ is formed where the original cell (CFU) was deposited. When you transfer this colony to a new sterile _____, using _____ techniques, you obtain a _____ _____.
Correct Answer(s)
single cell, divide, two, four, eight, colony, medium, aseptic, pure culture - 29.
The size of the zone of inhibition is determined by ________.
Correct Answer(s)
the diameter of the halo - 30.
There will be a clear area (measured as the diameter in millimeters) surrounding the antibiotic disk when the antibiotic is effective in controlling (inhibiting) or killing the bacteria tested, this area is named ________.
Correct Answer(s)
the zone of inhibition - 31.
The Kirby-Bauer Method is also called ______. This test is a valuable tool for measuring the _____ of antimicrobials against pathogenic microorganisms. All aspects of the Kirby-Bauer Method are _____ to ensure reliable results.
Correct Answer(s)
disk diffusion test, effectiveness, standardized - 32.
The letters on the antibiotic disks represent the ______ while the numbers represent the ______.
Correct Answer(s)
name of the antibiotic, antibiotic concentration - 33.
The size of the clear zone that appears around a disk where growth has been inhibited depends upon a few "things" except:
Correct Answer(s)
thickness of the culture medium - 34.
The Kirby-Bauer Test (Disk Diffusion Test) measures the effectiveness of agents against pathogenic microorganisms. Paper disks impregnated with specified amount of an agent (printed on the disk) are dispensed onto a plate inoculated with a bacterial lawn - these agents used are:
Correct Answer(s)
antibiotics - 35.
Control of microbes is very challenging. Microbes are everywhere and are very adaptable, therefore, this is of general concern and there's a need to overcome this challenge, a need to:
Correct Answer(s)
prevent contamination and attempt to control or kill the microbes - 36.
Eosin Methylene Blue (EMB) Agar is a _____ medium for some Gram _____ bacteria (inhibiting the growth of Gram _____ bacteria).
Correct Answer(s)
selective, negative, positive - 37.
MacConkey Agar is a _____ medium for the _____ family based on the ability to ferment _____.
Correct Answer(s)
selective, Enterobacteriaceae, lactose - 38.
Eosin Methylene Blue (EMB) Agar is a _____ medium, because it allows for differentiation of lactose and non-lactose _____.
Correct Answer(s)
differential, fermenters - 39.
Bile salts and crystal violet make MacConkey Agar a _____ medium that inhibit the growth of Gram _____ bacteria.
Correct Answer(s)
selective, positive - 40.
A non-lactose using organism would produce colonies of what color on EMB agar?
Correct Answer(s)
clear
same color as the medium - 41.
Bacterial morphology:
Correct Answer(s)
vibrio - 42.
Bacterial morphology:
Correct Answer(s)
spirochete - 43.
Bacterial Morphology:
Correct Answer(s)
spirillum - 44.
Bacterial Morphology:
Correct Answer(s)
pleomorphic - 45.
Bacterial Morphology:
Correct Answer(s)
coccus - 46.
Bacterial Morphology:
Correct Answer(s)
coccobacillus - 47.
Bacterial Morphology:
Correct Answer(s)
bacillus
.JPG "Bacterial Morphology:")
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