Microbiology Lab Mid-term

48 Questions | Attempts: 540
Please wait...
Question 1 / 49
🏆 Rank #-- ▾
Score 0/100

1. The lab coat is for the protection of the student and must be worn at all times in the lab. (True/False)

Submit
Please wait...
About This Quiz
Microbiology Lab Mid-term - Quiz

Tallahassee Community College MCB2004L Mid-term review

2.

What first name or nickname would you like us to use?

You may optionally provide this to label your report, leaderboard, or certificate.

2. The lab bench should be wiped down with disinfectant at the end of class. (True/False)

Submit

3. The wearing of open toed shoes is permitted in the microbiology lab. (True/False)

Submit

4. Hands should be washed after entering the room. (True/False)

Submit

5. Food, eating, drinking or applying make-up is not permitted in the lab as it could be hazardous to your health. (True/False)

Submit

6. I am allowed to take my cell phone to my desk. (True/False)

Submit

7. It is not necessary to wash hands before leaving the room. (True/False)

Submit

8. There is no reason to be concerned with the cultures used in lab as they are all non-pathogenic. (True/False)

Submit

9. A gram positive reaction gives a pink/red color. (True/False)

Submit

10. Used culture material (plates, swabs, toothpicks) should be disposed of in the trash can. (True/False)

Submit

11. A _____ is a liquid medium usually used for rapid growth or dilutions in test tubes or flasks.

Submit

12. The only personal things that I should take to my lab bench is my notepad, writing instrument, manual and atlas. (True/False)

Submit

13. One genus of human pathogenic bacteria, that is acid-fast is _____.

Submit

14. For the Gram Staining procedure: Match the function from the first column with the chemical that has that function from the second column.

Submit

15. Bacterial Morphology:

Submit

16. The lens that focus light through the specimen is called the _____.

Submit

17. The eyepiece of a microscope that remagnifies the image formed by the objective lens is called the _____.

Submit

18. Bacterial Morphology:

Submit

19. The Kirby-Bauer Test (Disk Diffusion Test) measures the effectiveness of agents against pathogenic microorganisms. Paper disks impregnated with specified amount of an agent (printed on the disk) are dispensed onto a plate inoculated with a bacterial lawn - these agents used are:

Submit

20. Bile salts and crystal violet make MacConkey Agar a _____ medium that inhibit the growth of Gram _____ bacteria.

Submit

21. Bacterial specimens are very _____ as well as _____, therefore we apply stain(s) to the cells to improve _____, allowing them to become more _____ under the microscope.

Submit

22. Eosin Methylene Blue (EMB) Agar is a _____ medium for some Gram _____ bacteria (inhibiting the growth of Gram _____ bacteria).

Submit

23. Bacterial morphology:

Submit

24. Bacterial morphology:

Submit

25. Eosin Methylene Blue (EMB) Agar is a _____ medium, because it allows for differentiation of lactose and non-lactose _____.

Submit

26. A ______ is a medium that inhibits the growth of certain microorganisms and therefore favors the growth of desired microorganisms

Submit

27. When working with Cultures in Petri Plates, some rules to keep in mind: a. Before incubating a plate, make sure you _____ it, also _____ it with a strip of _____; b. Incubate the plate in an _____ position, in the plastic bin designated for our class.

Submit

28. In theory, a ______ ______ is deposited on solid nutrient medium and it begins to _____: one cell makes two, _____ make _____, four make _____ (exponential growth) and keeps growing. From these cells, a bacterial _____ is formed where the original cell (CFU) was deposited. When you transfer this colony to a new sterile _____, using _____ techniques, you obtain a _____ _____.

Submit

29. A _____ is a medium with a thickener that allows growth of colonies on the surface only

Submit

30. A non-lactose using organism would produce colonies of what color on EMB agar?

Submit

31. You should carry the microscope with _____ hands (one under the _____ and one around the _____ ).

Submit

32. Bacterial Morphology:

Submit

33. If you can focus your slide under the lower powers, but are unable to focus under the higher powers (40x and oil), one likely reason is:

Submit

34. Bacterial Morphology:

Submit

35. MacConkey Agar is a _____ medium for the _____ family based on the ability to ferment _____.

Submit

36. Bacterial Morphology:

Submit

37. A _________ is a medium used to distinguish among organisms based on physical differences shown by the colony.

Submit

38. Acid-fast bacterial cells do not _____ because their cells have large amounts of _____ that repels the water-based dyes of the Gram stain.

Submit

39. A ______ is a medium used to determine motility of a bacterium

Submit

40. The Kirby-Bauer Method is also called ______. This test is a valuable tool for measuring the _____ of antimicrobials against pathogenic microorganisms. All aspects of the Kirby-Bauer Method are _____ to ensure reliable results.

Submit

41. Two reasons why you should clean your microscope before and after use are:

Submit

42. In our lab, used slides should be disposed of in the ______.

Submit

43. The size of the zone of inhibition is determined by ________.

Submit

44. The letters on the antibiotic disks represent the ______ while the numbers represent the ______.

Submit

45. The size of the clear zone that appears around a disk where growth has been inhibited depends upon a few "things" except:  

Submit

46. There will be a clear area (measured as the diameter in millimeters) surrounding the antibiotic disk when the antibiotic is effective in controlling (inhibiting) or killing the bacteria tested, this area is named ________.

Submit

47. Control of microbes is very challenging. Microbes are everywhere and are very adaptable, therefore, this is of general concern and there's a need to overcome this challenge, a need to:

Submit

48. When you first get your microscope and before storage, you should _____ all parts; always store the 'scope' on the _____ power objective lens; and also, turn the _____ off when the 'scope' is not being used for observation, as well as for storage.

Submit
×
Saved
Thank you for your feedback!
View My Results
Cancel
  • All
    All (48)
  • Unanswered
    Unanswered ()
  • Answered
    Answered ()
The lab coat is for the protection of the student and must be worn at...
The lab bench should be wiped down with disinfectant at the end of...
The wearing of open toed shoes is permitted in the microbiology lab....
Hands should be washed after entering the room. (True/False)
Food, eating, drinking or applying make-up is not permitted in the lab...
I am allowed to take my cell phone to my desk. (True/False)
It is not necessary to wash hands before leaving the room....
There is no reason to be concerned with the cultures used in lab as...
A gram positive reaction gives a pink/red color. (True/False)
Used culture material (plates, swabs, toothpicks) should be disposed...
A _____ is a liquid medium usually used for rapid growth or dilutions...
The only personal things that I should take to my lab bench is my...
One genus of human pathogenic bacteria, that is acid-fast is _____.
For the Gram Staining procedure: Match the function from the first...
Bacterial Morphology:
The lens that focus light through the specimen is called the _____.
The eyepiece of a microscope that remagnifies the image formed by the...
Bacterial Morphology:
The Kirby-Bauer Test (Disk Diffusion Test) measures the effectiveness...
Bile salts and crystal violet make MacConkey Agar a _____ medium that...
Bacterial specimens are very _____ as well as _____, therefore we...
Eosin Methylene Blue (EMB) Agar is a _____ medium for some Gram _____...
Bacterial morphology:
Bacterial morphology:
Eosin Methylene Blue (EMB) Agar is a _____ medium, because it allows...
A ______ is a medium that inhibits the growth of certain...
When working with Cultures in Petri Plates, some rules to keep in...
In theory, a ______ ______ is deposited on solid nutrient medium and...
A _____ is a medium with a thickener that allows growth of colonies on...
A non-lactose using organism would produce colonies of what color on...
You should carry the microscope with _____ hands (one under the _____...
Bacterial Morphology:
If you can focus your slide under the lower powers, but are unable to...
Bacterial Morphology:
MacConkey Agar is a _____ medium for the _____ family based on the...
Bacterial Morphology:
A _________ is a medium used to distinguish among organisms based on...
Acid-fast bacterial cells do not _____ because their cells have large...
A ______ is a medium used to determine motility of a bacterium
The Kirby-Bauer Method is also called ______. This test is a valuable...
Two reasons why you should clean your microscope before and after use...
In our lab, used slides should be disposed of in the ______.
The size of the zone of inhibition is determined by ________.
The letters on the antibiotic disks represent the ______ while the...
The size of the clear zone that appears around a disk where growth has...
There will be a clear area (measured as the diameter in millimeters)...
Control of microbes is very challenging. Microbes are everywhere and...
When you first get your microscope and before storage, you should...
play-Mute sad happy unanswered_answer up-hover down-hover success oval cancel Check box square blue
Alert!