Microbiology Lab Mid-term

Hands should be washed after entering the room. (True/False)

There is no reason to be concerned with the cultures used in lab as they are all non-pathogenic. (True/False)

The lab bench should be wiped down with disinfectant at the end of class. (True/False)

The only personal things that I should take to my lab bench is my notepad, writing instrument, manual and atlas. (True/False)

Used culture material (plates, swabs, toothpicks) should be disposed of in the trash can. (True/False)

The lab coat is for the protection of the student and must be worn at all times in the lab. (True/False)

Food, eating, drinking or applying make-up is not permitted in the lab as it could be hazardous to your health. (True/False)

The wearing of open toed shoes is permitted in the microbiology lab. (True/False)

I am allowed to take my cell phone to my desk. (True/False)

It is not necessary to wash hands before leaving the room. (True/False)

You should carry the microscope with _____ hands (one under the _____ and one around the _____ ).

The eyepiece of a microscope that remagnifies the image formed by the objective lens is called the _____.

If you can focus your slide under the lower powers, but are unable to focus under the higher powers (40x and oil), one likely reason is:

The lens that focus light through the specimen is called the _____.

Two reasons why you should clean your microscope before and after use are:

When you first get your microscope and before storage, you should _____ all parts; always store the 'scope' on the _____ power objective lens; and also, turn the _____ off when the 'scope' is not being used for observation, as well as for storage.

Acid-fast bacterial cells do not _____ because their cells have large amounts of _____ that repels the water-based dyes of the Gram stain.

A gram positive reaction gives a pink/red color. (True/False)

Bacterial specimens are very _____ as well as _____, therefore we apply stain(s) to the cells to improve _____, allowing them to become more _____ under the microscope.

One genus of human pathogenic bacteria, that is acid-fast is _____.

In our lab, used slides should be disposed of in the ______.

When working with Cultures in Petri Plates, some rules to keep in mind: a. Before incubating a plate, make sure you _____ it, also _____ it with a strip of _____; b. Incubate the plate in an _____ position, in the plastic bin designated for our class.

A _________ is a medium used to distinguish among organisms based on physical differences shown by the colony.

A ______ is a medium used to determine motility of a bacterium

A _____ is a liquid medium usually used for rapid growth or dilutions in test tubes or flasks.

A _____ is a medium with a thickener that allows growth of colonies on the surface only

A ______ is a medium that inhibits the growth of certain microorganisms and therefore favors the growth of desired microorganisms

In theory, a ______ ______ is deposited on solid nutrient medium and it begins to _____: one cell makes two, _____ make _____, four make _____ (exponential growth) and keeps growing. From these cells, a bacterial _____ is formed where the original cell (CFU) was deposited. When you transfer this colony to a new sterile _____, using _____ techniques, you obtain a _____ _____.

The size of the zone of inhibition is determined by ________.

There will be a clear area (measured as the diameter in millimeters) surrounding the antibiotic disk when the antibiotic is effective in controlling (inhibiting) or killing the bacteria tested, this area is named ________.

The Kirby-Bauer Method is also called ______. This test is a valuable tool for measuring the _____ of antimicrobials against pathogenic microorganisms. All aspects of the Kirby-Bauer Method are _____ to ensure reliable results.

The letters on the antibiotic disks represent the ______ while the numbers represent the ______.

The size of the clear zone that appears around a disk where growth has been inhibited depends upon a few "things" except:  

The Kirby-Bauer Test (Disk Diffusion Test) measures the effectiveness of agents against pathogenic microorganisms. Paper disks impregnated with specified amount of an agent (printed on the disk) are dispensed onto a plate inoculated with a bacterial lawn - these agents used are:

Control of microbes is very challenging. Microbes are everywhere and are very adaptable, therefore, this is of general concern and there's a need to overcome this challenge, a need to:

Eosin Methylene Blue (EMB) Agar is a _____ medium for some Gram _____ bacteria (inhibiting the growth of Gram _____ bacteria).

MacConkey Agar is a _____ medium for the _____ family based on the ability to ferment _____.

Eosin Methylene Blue (EMB) Agar is a _____ medium, because it allows for differentiation of lactose and non-lactose _____.

Bile salts and crystal violet make MacConkey Agar a _____ medium that inhibit the growth of Gram _____ bacteria.

A non-lactose using organism would produce colonies of what color on EMB agar?

Bacterial morphology:

Bacterial morphology:

Bacterial Morphology:

Bacterial Morphology:

Bacterial Morphology:

Bacterial Morphology:

Bacterial Morphology: