Microbiology Lab Mid-term
48 Questions
| Total Attempts: 515
Tallahassee Community College MCB2004L Mid-term review
Questions and Answers
- 1.Hands should be washed after entering the room. (True/False)
- 2.There is no reason to be concerned with the cultures used in lab as they are all non-pathogenic. (True/False)
- 3.The lab bench should be wiped down with disinfectant at the end of class. (True/False)
- 4.The only personal things that I should take to my lab bench is my notepad, writing instrument, manual and atlas. (True/False)
- 5.Used culture material (plates, swabs, toothpicks) should be disposed of in the trash can. (True/False)
- 6.The lab coat is for the protection of the student and must be worn at all times in the lab. (True/False)
- 7.Food, eating, drinking or applying make-up is not permitted in the lab as it could be hazardous to your health. (True/False)
- 8.The wearing of open toed shoes is permitted in the microbiology lab. (True/False)
- 9.I am allowed to take my cell phone to my desk. (True/False)
- 10.It is not necessary to wash hands before leaving the room. (True/False)
- 11.You should carry the microscope with _____ hands (one under the _____ and one around the _____ ).
- 12.The eyepiece of a microscope that remagnifies the image formed by the objective lens is called the _____.
- 13.If you can focus your slide under the lower powers, but are unable to focus under the higher powers (40x and oil), one likely reason is:
- A.
The condenser is not focused.
- B.
The light is too bright.
- C.
The ocular is dirty.
- D.
All the above are wrong.
- 14.The lens that focus light through the specimen is called the _____.
- 15.Two reasons why you should clean your microscope before and after use are:
- A.
To remove oil from the oil immersion lens.
- B.
To clean the condenser so that the light is pure.
- C.
To remove any harmful 'material' from the ocular.
- D.
To remove any pathogens from the objective.
- 16.When you first get your microscope and before storage, you should _____ all parts; always store the 'scope' on the _____ power objective lens; and also, turn the _____ off when the 'scope' is not being used for observation, as well as for storage.
- 17.Acid-fast bacterial cells do not _____ because their cells have large amounts of _____ that repels the water-based dyes of the Gram stain.
- 18.A gram positive reaction gives a pink/red color. (True/False)
- 19.Bacterial specimens are very _____ as well as _____, therefore we apply stain(s) to the cells to improve _____, allowing them to become more _____ under the microscope.
- 20.One genus of human pathogenic bacteria, that is acid-fast is _____.
- 21.In our lab, used slides should be disposed of in the ______.
- 22.When working with Cultures in Petri Plates, some rules to keep in mind: a. Before incubating a plate, make sure you _____ it, also _____ it with a strip of _____; b. Incubate the plate in an _____ position, in the plastic bin designated for our class.
- 23.A _________ is a medium used to distinguish among organisms based on physical differences shown by the colony.
- 24.A ______ is a medium used to determine motility of a bacterium
- 25.A _____ is a liquid medium usually used for rapid growth or dilutions in test tubes or flasks.
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