Microbiology Lab Mid-Term

2.

The lab bench should be wiped down with disinfectant at the end of class. (True/False)

3.

The wearing of open toed shoes is permitted in the microbiology lab. (True/False)

4.

Hands should be washed after entering the room. (True/False)

5.

Food, eating, drinking or applying make-up is not permitted in the lab as it could be hazardous to your health. (True/False)

6.

I am allowed to take my cell phone to my desk. (True/False)

7.

It is not necessary to wash hands before leaving the room. (True/False)

8.

There is no reason to be concerned with the cultures used in lab as they are all non-pathogenic. (True/False)

9.

A gram positive reaction gives a pink/red color. (True/False)

10.

Used culture material (plates, swabs, toothpicks) should be disposed of in the trash can. (True/False)

11.

A _____ is a liquid medium usually used for rapid growth or dilutions in test tubes or flasks.

12.

The only personal things that I should take to my lab bench is my notepad, writing instrument, manual and atlas. (True/False)

13.

One genus of human pathogenic bacteria, that is acid-fast is _____.

14.

Bacterial Morphology:

15.

The lens that focus light through the specimen is called the _____.

16.

The eyepiece of a microscope that remagnifies the image formed by the objective lens is called the _____.

17.

The Kirby-Bauer Test (Disk Diffusion Test) measures the effectiveness of agents against pathogenic microorganisms. Paper disks impregnated with specified amount of an agent (printed on the disk) are dispensed onto a plate inoculated with a bacterial lawn - these agents used are:

18.

Bacterial Morphology:

19.

Bile salts and crystal violet make MacConkey Agar a _ medium that inhibit the growth of Gram _ bacteria.

20.

Bacterial specimens are very ___ as well as _, therefore we apply stain(s) to the cells to improve _, allowing them to become more ___ under the microscope.

21.

Eosin Methylene Blue (EMB) Agar is a ___ medium for some Gram _ bacteria (inhibiting the growth of Gram ___ bacteria).

22.

Bacterial morphology:

23.

Bacterial morphology:

24.

Eosin Methylene Blue (EMB) Agar is a _ medium, because it allows for differentiation of lactose and non-lactose _.

25.

A ______ is a medium that inhibits the growth of certain microorganisms and therefore favors the growth of desired microorganisms

26.

When working with Cultures in Petri Plates, some rules to keep in mind: a. Before incubating a plate, make sure you ___ it, also _ it with a strip of _; b. Incubate the plate in an ___ position, in the plastic bin designated for our class.

27.

In theory, a __ is deposited on solid nutrient medium and it begins to _: one cell makes two, _ make _, four make _ (exponential growth) and keeps growing. From these cells, a bacterial _ is formed where the original cell (CFU) was deposited. When you transfer this colony to a new sterile _, using _ techniques, you obtain a _ ___.

28.

A _____ is a medium with a thickener that allows growth of colonies on the surface only

29.

A non-lactose using organism would produce colonies of what color on EMB agar?

30.

You should carry the microscope with ___ hands (one under the _ and one around the ___ ).

31.

Bacterial Morphology:

32.

If you can focus your slide under the lower powers, but are unable to focus under the higher powers (40x and oil), one likely reason is:

The condenser is not focused.
The light is too bright.
The ocular is dirty.
All the above are wrong.

Correct Answer

A. All the above are wrong.

33.

MacConkey Agar is a ___ medium for the _ family based on the ability to ferment ___.

Correct Answer
selective, Enterobacteriaceae, lactose

34.

Bacterial Morphology:

Correct Answer
spirillum

35.

Bacterial Morphology:

Correct Answer
coccobacillus

36.

A _________ is a medium used to distinguish among organisms based on physical differences shown by the colony.

Correct Answer
differential media

37.

Acid-fast bacterial cells do not _ because their cells have large amounts of _ that repels the water-based dyes of the Gram stain.

Correct Answer
gram stain, waxy material

38.

A ______ is a medium used to determine motility of a bacterium

Correct Answer
semi-solid media

39.

The Kirby-Bauer Method is also called ____. This test is a valuable tool for measuring the _ of antimicrobials against pathogenic microorganisms. All aspects of the Kirby-Bauer Method are ___ to ensure reliable results.

Correct Answer
disk diffusion test, effectiveness, standardized

40.

Two reasons why you should clean your microscope before and after use are:

To remove oil from the oil immersion lens.
To clean the condenser so that the light is pure.
To remove any harmful 'material' from the ocular.
To remove any pathogens from the objective.

Correct Answer(s)

A. To remove oil from the oil immersion lens.
A. To remove any harmful 'material' from the ocular.

41.

In our lab, used slides should be disposed of in the ______.

Correct Answer(s)
labeled small white bucket

42.

The size of the zone of inhibition is determined by ________.

Correct Answer(s)
the diameter of the halo

43.

The letters on the antibiotic disks represent the while the numbers represent the .

Correct Answer(s)
name of the antibiotic, antibiotic concentration

44.

The size of the clear zone that appears around a disk where growth has been inhibited depends upon a few "things" except:

Correct Answer(s)
thickness of the culture medium

45.

There will be a clear area (measured as the diameter in millimeters) surrounding the antibiotic disk when the antibiotic is effective in controlling (inhibiting) or killing the bacteria tested, this area is named ________.

Correct Answer(s)
the zone of inhibition

46.

Control of microbes is very challenging. Microbes are everywhere and are very adaptable, therefore, this is of general concern and there's a need to overcome this challenge, a need to:

Correct Answer(s)
prevent contamination and attempt to control or kill the microbes

47.

When you first get your microscope and before storage, you should ___ all parts; always store the 'scope' on the _ power objective lens; and also, turn the ___ off when the 'scope' is not being used for observation, as well as for storage.

Correct Answer(s)
clean, lowest, light

Microbiology Lab Mid-term

The lab coat is for the protection of the student and must be worn at all times in the lab. (True/False)

Quiz Preview

The lab bench should be wiped down with disinfectant at the end of class. (True/False)

The wearing of open toed shoes is permitted in the microbiology lab. (True/False)

Hands should be washed after entering the room. (True/False)

Food, eating, drinking or applying make-up is not permitted in the lab as it could be hazardous to your health. (True/False)

I am allowed to take my cell phone to my desk. (True/False)

It is not necessary to wash hands before leaving the room. (True/False)

There is no reason to be concerned with the cultures used in lab as they are all non-pathogenic. (True/False)

A gram positive reaction gives a pink/red color. (True/False)

Used culture material (plates, swabs, toothpicks) should be disposed of in the trash can. (True/False)

A _____ is a liquid medium usually used for rapid growth or dilutions in test tubes or flasks.

The only personal things that I should take to my lab bench is my notepad, writing instrument, manual and atlas. (True/False)

One genus of human pathogenic bacteria, that is acid-fast is _____.

Bacterial Morphology:

The lens that focus light through the specimen is called the _____.

The eyepiece of a microscope that remagnifies the image formed by the objective lens is called the _____.

The Kirby-Bauer Test (Disk Diffusion Test) measures the effectiveness of agents against pathogenic microorganisms. Paper disks impregnated with specified amount of an agent (printed on the disk) are dispensed onto a plate inoculated with a bacterial lawn - these agents used are:

Bacterial Morphology:

Bile salts and crystal violet make MacConkey Agar a _____ medium that inhibit the growth of Gram _____ bacteria.

Bacterial specimens are very _____ as well as _____, therefore we apply stain(s) to the cells to improve _____, allowing them to become more _____ under the microscope.

Eosin Methylene Blue (EMB) Agar is a _____ medium for some Gram _____ bacteria (inhibiting the growth of Gram _____ bacteria).

Bacterial morphology:

Bacterial morphology:

Eosin Methylene Blue (EMB) Agar is a _____ medium, because it allows for differentiation of lactose and non-lactose _____.

A ______ is a medium that inhibits the growth of certain microorganisms and therefore favors the growth of desired microorganisms

When working with Cultures in Petri Plates, some rules to keep in mind: a. Before incubating a plate, make sure you _____ it, also _____ it with a strip of _____; b. Incubate the plate in an _____ position, in the plastic bin designated for our class.

A _____ is a medium with a thickener that allows growth of colonies on the surface only

A non-lactose using organism would produce colonies of what color on EMB agar?

You should carry the microscope with _____ hands (one under the _____ and one around the _____ ).

Bacterial Morphology:

If you can focus your slide under the lower powers, but are unable to focus under the higher powers (40x and oil), one likely reason is:

MacConkey Agar is a _____ medium for the _____ family based on the ability to ferment _____.

Bacterial Morphology:

Bacterial Morphology:

A _________ is a medium used to distinguish among organisms based on physical differences shown by the colony.

Acid-fast bacterial cells do not _____ because their cells have large amounts of _____ that repels the water-based dyes of the Gram stain.

A ______ is a medium used to determine motility of a bacterium

The Kirby-Bauer Method is also called ______. This test is a valuable tool for measuring the _____ of antimicrobials against pathogenic microorganisms. All aspects of the Kirby-Bauer Method are _____ to ensure reliable results.

Two reasons why you should clean your microscope before and after use are:

In our lab, used slides should be disposed of in the ______.

The size of the zone of inhibition is determined by ________.

The letters on the antibiotic disks represent the ______ while the numbers represent the ______.

The size of the clear zone that appears around a disk where growth has been inhibited depends upon a few "things" except:

There will be a clear area (measured as the diameter in millimeters) surrounding the antibiotic disk when the antibiotic is effective in controlling (inhibiting) or killing the bacteria tested, this area is named ________.

Control of microbes is very challenging. Microbes are everywhere and are very adaptable, therefore, this is of general concern and there's a need to overcome this challenge, a need to:

When you first get your microscope and before storage, you should _____ all parts; always store the 'scope' on the _____ power objective lens; and also, turn the _____ off when the 'scope' is not being used for observation, as well as for storage.

Bile salts and crystal violet make MacConkey Agar a _ medium that inhibit the growth of Gram _ bacteria.

Bacterial specimens are very ___ as well as _, therefore we apply stain(s) to the cells to improve _, allowing them to become more ___ under the microscope.

Eosin Methylene Blue (EMB) Agar is a ___ medium for some Gram _ bacteria (inhibiting the growth of Gram ___ bacteria).

Eosin Methylene Blue (EMB) Agar is a _ medium, because it allows for differentiation of lactose and non-lactose _.

When working with Cultures in Petri Plates, some rules to keep in mind: a. Before incubating a plate, make sure you ___ it, also _ it with a strip of _; b. Incubate the plate in an ___ position, in the plastic bin designated for our class.

You should carry the microscope with ___ hands (one under the _ and one around the ___ ).

MacConkey Agar is a ___ medium for the _ family based on the ability to ferment ___.

Acid-fast bacterial cells do not _ because their cells have large amounts of _ that repels the water-based dyes of the Gram stain.

The Kirby-Bauer Method is also called ____. This test is a valuable tool for measuring the _ of antimicrobials against pathogenic microorganisms. All aspects of the Kirby-Bauer Method are ___ to ensure reliable results.

The letters on the antibiotic disks represent the while the numbers represent the .

When you first get your microscope and before storage, you should ___ all parts; always store the 'scope' on the _ power objective lens; and also, turn the ___ off when the 'scope' is not being used for observation, as well as for storage.