Light Microscope; Handling & care

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The correct answer is "stage" because it is the flat platform where you place your slides.

The explanation for the given correct answer is that when a microscope has a lamp as the light source, the bulb of the lamp can get very hot. Therefore, it is important to be careful and avoid touching the bulb to prevent any burns or accidents.

During transportation of a compound light microscope, it is recommended to place your hands on both the arm and the base. This ensures a secure grip and prevents any accidental damage or movement of the microscope. Holding the arm provides stability and control, while supporting the base helps to distribute the weight evenly and prevent any imbalance. By holding both the arm and the base, you can safely transport the microscope without risking any potential harm to yourself or the equipment.

The textbook and biology instructor recommend using only 1 drop of water to cover a specimen.

The arm supports the tube and connects it to the base.

The specimen should be placed in the center of the microscope slide. Placing it in the center ensures that it is evenly distributed on the slide, allowing for easy observation and focusing under the microscope. Placing it on the left or right margin may result in an uneven distribution of the specimen, making it difficult to view. Placing it on top of the cover slip is also not ideal, as the cover slip is typically used to protect the specimen and prevent it from drying out, rather than as a position for observation.

The part of the microscope that holds the microscope slide on the stage is called stage clips or stage clip.

The lens that you look through when observing a specimen is called the eyepiece or ocular.

The eyepiece and objective lens of a compound light microscope should be cleaned every time it is used to ensure clear and accurate observations. Cleaning these components removes any dust, debris, or smudges that may affect the quality of the image seen through the microscope. Regular cleaning helps maintain the functionality and performance of the microscope.

The total magnification is determined by multiplying the magnification of the ocular lens (10X) by the magnification of the objective lens (40X). Therefore, the total magnification is 10X * 40X = 400X.

Locking the high power objective into viewing position first is not the correct procedure when observing a specimen with a compound light microscope. The correct procedure is to start with the lowest power objective (usually the scanning objective), focus on the specimen, and then gradually increase the magnification by rotating the nosepiece to the next higher power objective. Locking the high power objective into viewing position first would make it difficult to focus on the specimen and may result in damage to the microscope or specimen.

The disc diaphragm in a compound light microscope is used to control the amount of light passing through the specimen. By adjusting the disc diaphragm, the user can increase or decrease the intensity of the light, which allows for better visualization and clarity of the specimen. This feature is particularly useful when observing specimens with different levels of transparency or when trying to enhance specific details of the specimen.

To secure a prepared slide on the stage, it is necessary to place it over the hole in the stage and then use the stage clips to secure it. The hole in the stage provides a designated area where the slide can be properly positioned and held in place. This ensures that the slide remains stable during observation and prevents it from moving or falling off the stage.

The large knob used for focusing the image under low-power is called the coarse adjustment knob. This knob is used to make rough adjustments to the focus of the image, allowing for clearer viewing of the specimen.

When you look through the eyepiece, you can adjust the amount of light that passes through the specimen by moving the diaphragm. The diaphragm is a part of the microscope that controls the amount of light that reaches the specimen. By adjusting the diaphragm, you can increase or decrease the brightness of the light, which can help improve the visibility and clarity of the specimen under the microscope.

The statement is false because the coarse adjustment should not be used when viewing through the high-power objective. The coarse adjustment is used to initially focus the specimen when using the low-power objective, while the fine adjustment is used for fine-tuning the focus when using the high-power objective. Using the coarse adjustment with the high-power objective can result in damage to the microscope or the specimen.

The correct sequence to use the objective lens to observe a specimen using a compound light microscope is scanning, low, and high. The scanning objective lens is used first to locate the specimen and get a general overview. Then, the low-power objective lens is used to obtain a more detailed view of the specimen. Finally, the high-power objective lens is used to observe the specimen at the highest magnification for fine details.

The stage stop is a crucial component of the compound light microscope as it serves to prevent the objective lens from cracking the coverslip and microscope slide. This is important because the objective lens is in close proximity to the slide, and any contact or pressure could potentially damage or break the coverslip. The stage stop acts as a physical barrier, ensuring that the objective lens does not come into direct contact with the slide, thus preventing any potential damage. Therefore, the statement is true.

The correct position for the fine adjustment knob before using a compound light microscope is in the middle (balanced) position. This is because the fine adjustment knob is used to make small, precise adjustments to the focus of the microscope. By starting in the middle position, you ensure that the focus is not too far in one direction, making it easier to fine-tune and achieve a clear image.

When the letter "e" is placed upside down on the microscope slide, it will appear upside down through the ocular. This is because the microscope lenses invert the image, causing objects to appear flipped when viewed through the microscope. Therefore, the correct answer is "Upside down on the microscope slide."

Objective lenses are the low power and high power lenses that magnify the object. These lenses are commonly used in microscopes to provide different levels of magnification. The low power objective lens has a lower magnification power and is typically used to view larger specimens or to get a wider field of view. On the other hand, the high power objective lens has a higher magnification power and is used to view smaller and more detailed specimens. Both lenses together allow for a range of magnification options when observing objects under a microscope.

Observing specimens through the ocular of a compound light microscope with both eyes open is considered a good practice because it allows for better depth perception and a wider field of view. This helps in accurately observing and interpreting the details of the specimen being viewed. Additionally, using both eyes can reduce eye strain and fatigue during prolonged periods of observation.

Positioning the coverslip at the end of the drop of water and at a 45 degree angle allows for a smooth and gradual placement, reducing the likelihood of trapping air bubbles underneath. Placing it at the edge of the drop or at a different angle may cause the coverslip to slide or push the water, creating air bubbles. Positioning it at the far right of the microscope slide is unrelated to reducing air bubbles.

When observing a specimen with a compound light microscope, you lock the scanning objective lens into viewing position first. The scanning objective lens has the lowest magnification power, typically around 4x or 5x. This allows you to quickly scan the specimen and locate the area of interest before switching to higher magnification lenses. Locking the scanning lens first ensures that you have a wider field of view and can easily navigate and focus on the specimen.

Bibulous paper is a highly absorbent paper that is commonly used in laboratories to remove excess liquid from surfaces. In the context of a wet mount preparation, bibulous paper can be used to quickly and effectively draw out excessive water from the cover slip and specimen. This helps to prevent the water from interfering with the microscopic observation and ensures a clear view of the specimen.

When using the fine adjustment knob on the compound light microscope, the high power objective lens is locked into viewing position. This is because the high power objective lens provides the highest magnification and therefore requires the most precise adjustment to focus on the specimen. The fine adjustment knob allows for small, incremental movements of the high power objective lens to achieve a clear and detailed image of the specimen.

The coarse adjustment knob on a compound light microscope is used to bring the specimen into rough focus. It moves the stage up and down, allowing the user to quickly adjust the focus. The low power objective lens is locked into viewing position because it is the lens that is closest to the specimen and provides the initial magnification. The other objective lenses, such as the high power and oil immersion objectives, can be rotated into place once the specimen is roughly in focus using the coarse adjustment knob.

The compound light microscope should be placed at least 5 centimeters from the edge of the lab table. This is important to ensure stability and prevent accidental knocking or falling of the microscope. Placing it too close to the edge increases the risk of damage to the microscope or potential injury.

Adding a stain to a wet mount prepared specimen after placing the coverslip on the specimen is the correct answer because adding the stain before placing the coverslip would dilute the stain and make it less effective. Removing the coverslip after the specimen is prepared would disturb the specimen and potentially ruin the slide. Adding the stain before placing the specimen on the microscope slide is not necessary as the stain is typically added directly to the specimen on the slide.