Light Microscope; Handling & care

30 Questions | Total Attempts: 873

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Light Microscope; Handling & care - Quiz

Welcome to the electronic science frontier classroom of the 21st century. Microscopes are very useful tools used by a wide spectrum of scientists. They extend human vision by making enlarged images of very small objects. The compound light microscope becomes one of the basic tools in high school and college biology classes. This test enables you to determine how well you have been taught about the care and handling of a compound light microscope and procedure for making a wet mount microscope slide. I wish you well in your future and hoping you will have a better appreciation for the life sciences. Good Luck!


Questions and Answers
  • 1. 
    How is excessive water drawn from the cover slip and specimen of a "wet mount preparation"?  
    • A. 

      Lick the excessive water from the microscope slide

    • B. 

      Use a pipet to remove the excessive water

    • C. 

      Use a sponge to remove the excessive water

    • D. 

      Use bibulous paper for faster results

  • 2. 
    What is the total magnification of a 10X ocular and 40X high power objective lens?
    • A. 

      4X

    • B. 

      10X

    • C. 

      400X

    • D. 

      4 000X

  • 3. 
    What part of a compound light microscope is used to control the amount of light passing through the specimen?
    • A. 

      Low Power Objective

    • B. 

      Stage Clips

    • C. 

      Nose-piece

    • D. 

      Disc Diaphragm

  • 4. 
    Where are your hands placed on the compound light microscope during transportation?  
    • A. 

      Arm only

    • B. 

      Base only

    • C. 

      Arm and Base

    • D. 

      Base and Nosepiece

  • 5. 
    When the letter "e" is placed ointo a "wet mount" microscope slide facing upright position. How will you observe the letter "e" through the ocular?    
    • A. 

      Correct position as you placed it on the microscope slide

    • B. 

      Upside down on the microscope slide

    • C. 

      The letter "e" is facing toward the left stage clip

    • D. 

      The letter "e" is facing toward the right stage clip

    • E. 

      The letter "e" is not visible

  • 6. 
    When should you clean the eyepiece and objective lens of your compound light microscope?
    • A. 

      Every time you use the compound light microscope

    • B. 

      Once a week (if the microscope is used every laboratory) period)

    • C. 

      Twice a week (if the microscope is used every laboratory) period)

    • D. 

      You allow the biology teacher to clean the microscope

    • E. 

      The microscope company cleans the lens system

  • 7. 
    How should you reduce the number of air bubbles under the cover slip?  
    • A. 

      Position coverslip at the edge of the drop of water and 90 degree angle

    • B. 

      Position coverslip at the end of the drop of water and 45 degree angle

    • C. 

      Position coverslip at the end of the drop of water and 30 degree angle

    • D. 

      Position coverslip at the far right of the microscope slide

  • 8. 
    What microscope part holds the microscope slide on the stage?  (hint: 2 words) Please enter your answer(s) in all lower case letters.
  • 9. 
    When using the coarse adjustment knob on the compound light microscope. Which objective lens is locked into viewing position?
    • A. 

      Low Power Objective

    • B. 

      Eyepiece Objective

    • C. 

      Revolving Objective

    • D. 

      Electronic Objective

  • 10. 
    When using the fine adjustment knob on the compound light microscope. Which objective lens is locked into viewing position?
    • A. 

      Scanning Objective

    • B. 

      Low Power Objective

    • C. 

      High Power Objective

    • D. 

      Eyepiece Objective

  • 11. 
    When observing a specimen with a compound light microscope.  You lock the high power objective into viewing position first.
    • A. 

      True

    • B. 

      False

  • 12. 
    When observing a specimen with a compound light microscope.  You lock  _____ objective lens into viewing position first.
    • A. 

      Low power

    • B. 

      High power

    • C. 

      Scanning

    • D. 

      Research power

  • 13. 
    What is the correct sequence to use the objective lens to observe a specimen using a compound light microscope?
    • A. 

      Scanning - High - Low

    • B. 

      Scanning - Low - High

    • C. 

      Low - Scanning - High

    • D. 

      High - Low - Scanning

  • 14. 
    The stage stop is part of the compound light microscope that prevents the objective lens from cracking the coverslip and microscope slide.
    • A. 

      True

    • B. 

      False

  • 15. 
    It is a good practice to observe specimens through the ocular of the compound light microscope with both eyes open.
    • A. 

      True

    • B. 

      False

  • 16. 
    When do you add a stain to a wet mount prepared specimen?
    • A. 

      After placing coverslip on specimen

    • B. 

      After adding water before coverslip

    • C. 

      Remove coverslip after specimen is prepared

    • D. 

      Before specimen is placed on microscope slide (company only)

  • 17. 
    Place a specimen that you wish to observe on the microscope slide. Which position should the specimen be placed?  (hint: This is a position on the microscope slide.)
    • A. 

      Left margin of microscope slide

    • B. 

      Right margin of microscope slide

    • C. 

      Center of microscope slide

    • D. 

      On top of the cover slip

  • 18. 
    You should place the compound light microscope on your lab table at least _____ centimeters from the edge of the table.
    • A. 

      2

    • B. 

      5

    • C. 

      10

    • D. 

      20

  • 19. 
    If your microscope has a lamp as the light source, be careful not to touch the bulb of the lamp. The bulb can get very hot.
    • A. 

      True

    • B. 

      False

  • 20. 
    Place a prepared slide over the _____ in the stage, and secure the slide with the stage clips.
    • A. 

      Stage stop screw

    • B. 

      Hole

    • C. 

      Left edge

    • D. 

      Right edge

  • 21. 
    When you look through the eyepiece, and move the _____ to adjust the amount of _____ that passes through the specimen.
    • A. 

      Coverslip : High-power objective

    • B. 

      Ocular : Light

    • C. 

      Diaphragm : Light

    • D. 

      Revolving Nosepiece : Diaphragm

  • 22. 
    What is the correct position for the fine adjustment knob, before you begin using your compound light microscope?
    • A. 

      Moved to the left of your microscope

    • B. 

      Moved to the right of your microscope

    • C. 

      In the middle (balanced) position

    • D. 

      It makes no difference on focusing

  • 23. 
    You can use the coarse adjustment when viewing through the high-power objective.
    • A. 

      True

    • B. 

      False

  • 24. 
    What supports the tube and connects it to the base? (NOTE: please enter your answer in lower case letters.)
  • 25. 
    What is the name of number 13 in the image below?
    • A. 

      Stage

    • B. 

      Stage Clips

    • C. 

      Eyepiece

    • D. 

      Nosepiece

    • E. 

      Light source

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