Lab 9 Control Of Microbial Growth

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1/12 Questions

Bacteriostatic:
- Agent that inhibits the growth of bacteria but doesn’t kill them
- Agents that reduce microbial growth but do not eliminate all microbes (food industry – used to clean cooking equipment)
- Chemical agents applied to inanimate objects. More harsh. Some may destroy endospores (steriliants or sporocides) (ex – ethylene oxide)

About This Quiz

Lab 9 Control of Microbial Growth explores essential microbiology lab techniques for controlling microbial growth. It assesses knowledge on antibiotic susceptibility, use of specific media, and effectiveness of disinfectants. Key organisms like E. Coli and S. Aureus are studied, relevant for students and professionals in microbiology and related fields.

2.

Bacteriocidal
- Chemical agents that are used to control microorganisms
- Chemical agents applied to inanimate objects. More harsh. Some may destroy endospores (steriliants or sporocides) (ex – ethylene oxide)
- Agent that kills bacterial cells
Correct Answer

A. Agent that kills bacterial cells

Explanation

Bacteriocidal refers to chemical agents that are used to control microorganisms, specifically those that kill bacterial cells. These agents are typically applied to inanimate objects and are more harsh compared to other chemical agents. Some bacteriocidal agents have the ability to destroy endospores, such as ethylene oxide, making them effective sterilizers or sporocides.

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3.

Sanitizers
- Agents that reduce microbial growth but do not eliminate all microbes (food industry – used to clean cooking equipment)
- Chemical agents applied to inanimate objects. More harsh. Some may destroy endospores (steriliants or sporocides) (ex – ethylene oxide)
- (alcohol, betadine) – can be applied to tissue, do not destroy endospores.
Correct Answer

A. Agents that reduce microbial growth but do not eliminate all microbes (food industry – used to clean cooking equipment)

Explanation

Sanitizers are chemical agents that are used in the food industry to clean cooking equipment. They work by reducing microbial growth on the surfaces they are applied to. However, they do not completely eliminate all microbes. Sanitizers are more harsh compared to other chemical agents and some may have the ability to destroy endospores, which are highly resistant forms of bacteria. Unlike sterilants or sporocides, such as ethylene oxide, sanitizers like alcohol or betadine can also be applied to tissue but they do not have the ability to destroy endospores.

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4.

Organism used
- Aspergillus sp., Penicillium sp., or Rhizopus sp.
- Serratia marcescens
- E.coli , S.Aureus, and P.Aeruginosa
Correct Answer

A. E.coli , S.Aureus, and P.Aeruginosa

Explanation

The organisms E.coli, S.Aureus, and P.Aeruginosa are commonly used in microbiology laboratories for various purposes. E.coli is a bacterium commonly found in the intestines of humans and animals and is often used as a model organism for genetic research. S.Aureus is a bacterium that can cause various infections in humans and is used in laboratory settings to study antimicrobial resistance. P.Aeruginosa is a versatile bacterium known for its ability to cause infections in immunocompromised individuals and is commonly used in research on biofilms and antibiotic resistance. These organisms are chosen for their ease of cultivation, well-studied characteristics, and relevance to human health.

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5.

Organisms:
- Aspergillus sp., Penicillium sp., or Rhizopus sp.
- Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli
- Serratia marcescens
Correct Answer

A. Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli

Explanation

The given answer includes Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. These are all bacterial species commonly found in various environments, including the human body. Staphylococcus aureus is a gram-positive bacterium that can cause infections, while Pseudomonas aeruginosa is a gram-negative bacterium known for its resistance to antibiotics. Escherichia coli is a gram-negative bacterium commonly found in the intestines of humans and animals. These bacteria are all potential pathogens and can cause various diseases in humans.

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6.

Kirby-bauer Test For
- Antibiotic Suceptibility
- Disinfectants
- Bateriostatic
Correct Answer

A. Antibiotic Suceptibility

Explanation

The Kirby-Bauer test is used to determine the susceptibility of bacteria to antibiotics. In this test, paper discs containing different antibiotics are placed on a culture of bacteria. The antibiotics diffuse into the surrounding agar, creating a concentration gradient. If the bacteria are susceptible to a particular antibiotic, there will be a clear zone around the disc where the bacteria have not grown. This indicates that the antibiotic has inhibited the growth of the bacteria. Therefore, the correct answer is "Antibiotic Susceptibility."

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7.

Turbidity
- MacFarland 0.5 turbidity aproximately 1.5 x 10^8 power baterial/ml antibiotic discs with a know potency
- 0.85% turbidity
- 1.0 5 turbidity
Correct Answer

A. MacFarland 0.5 turbidity aproximately 1.5 x 10^8 power baterial/ml antibiotic discs with a know potency

Explanation

The given answer states that the MacFarland 0.5 turbidity has approximately 1.5 x 10^8 power bacteria/ml and is used in combination with antibiotic discs that have a known potency. This suggests that the MacFarland 0.5 turbidity is a standard reference for measuring bacterial concentration and is commonly used in microbiology laboratories. The antibiotic discs with known potency are likely used to test the effectiveness of antibiotics against the bacteria at this specific turbidity level.

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8.

Antiseptics:
- (alcohol, betadine) – can be applied to tissue, do not destroy endospores.
- Chemical agents applied to inanimate objects. More harsh. Some may destroy endospores (steriliants or sporocides) (ex – ethylene oxide)
- Chemical agents that are used to control microorganisms
Correct Answer

A. (alcohol, betadine) – can be applied to tissue, do not destroy endospores.

Explanation

Antiseptics such as alcohol and betadine are used to kill or inhibit the growth of microorganisms on living tissue. They are safe to be applied to the skin and do not destroy endospores, which are highly resistant forms of bacteria. These antiseptics are less harsh compared to other chemical agents used on inanimate objects. Some chemical agents, known as steriliants or sporocides, like ethylene oxide, can destroy endospores and are used to achieve sterilization.

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9.

Disinfectants and Antiseptics
- Chemical agents applied to inanimate objects. More harsh. Some may destroy endospores (steriliants or sporocides) (ex – ethylene oxide)
- Chemical agents that are used to control microorganisms
- (alcohol, betadine) – can be applied to tissue, do not destroy endospores.
Correct Answer

A. Chemical agents that are used to control microorganisms

Explanation

Disinfectants and antiseptics are chemical agents that are used to control microorganisms. They are applied to inanimate objects and can be more harsh compared to other types of chemical agents. Some disinfectants and antiseptics have the ability to destroy endospores, making them effective sterilizing agents. Examples of such agents include ethylene oxide. Other disinfectants and antiseptics, like alcohol and betadine, can be applied to tissue but do not have the ability to destroy endospores.

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10.

Media used
- Agar 0.3 %
- Muller- hilton agar which has a spesific ph of 7.2-7.4 unifor depth 4mm
- Solid agar
Correct Answer

A. Muller- hilton agar which has a spesific ph of 7.2-7.4 unifor depth 4mm

Explanation

The given answer states that the media used is Muller-Hilton agar, which has a specific pH range of 7.2-7.4 and a uniform depth of 4mm. This information is important because it provides details about the type of agar used for the experiment. Agar is a substance used in laboratories to provide a solid surface for the growth of microorganisms. The specific pH range ensures optimal conditions for the growth of the desired microorganisms, while the uniform depth of 4mm ensures consistency in the results obtained.

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11.

Disinfectants:
- Agents that reduce microbial growth but do not eliminate all microbes (food industry – used to clean cooking equipment)
- (alcohol, betadine) – can be applied to tissue, do not destroy endospores.
- Chemical agents applied to inanimate objects. More harsh. Some may destroy endospores (steriliants or sporocides) (ex – ethylene oxide)
Correct Answer

A. Chemical agents applied to inanimate objects. More harsh. Some may destroy endospores (steriliants or sporocides) (ex – ethylene oxide)

Explanation

Disinfectants are chemical agents that are used to reduce microbial growth but do not eliminate all microbes. They are commonly used in the food industry to clean cooking equipment. Disinfectants like alcohol and betadine can be applied to tissue but do not destroy endospores. On the other hand, chemical agents applied to inanimate objects are more harsh and some of them, known as steriliants or sporocides, can destroy endospores. Ethylene oxide is an example of such a chemical agent.

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12.

Highly Controlled
- To measure the relative effective of various disinfectants and antiseptics against bacterial organism
- Chemical agents that are used to control microorganism
- Producere to ensure accurate and producible results
Correct Answer

A. Producere to ensure accurate and producible results

Explanation

The term "producere" refers to the process of ensuring accurate and producible results. In the context of the question, it implies that the use of highly controlled conditions is necessary in order to measure the relative effectiveness of disinfectants and antiseptics against bacterial organisms. This suggests that the accuracy and reproducibility of the results are crucial in evaluating the efficacy of these chemical agents in controlling microorganisms.

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Mar 20, 2023

Quiz Edited by
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Oct 27, 2010

Quiz Created by
Maer

Lab 9 Control Of Microbial Growth

Bacteriostatic:

Quiz Preview

Bacteriocidal

Sanitizers

Organism used

Organisms:

Kirby-bauer Test For

Turbidity

Antiseptics:

Disinfectants and Antiseptics

Media used

Disinfectants:

Highly Controlled