Bacteriology Quiz Questions And Answers

51 Questions | Total Attempts: 227

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Bacteriology Quiz Questions And Answers - Quiz

Questions and Answers
  • 1. 
    Week 1. Lecture. Subject of microbiology, its aim and short history Practical. Media, sterilization, bacterial cultures, anaerobic culture methods, pure cultures, microscopy, examination of native bacteria, staining of bacteria.  
  • 2. 
    Culture bacteria to use for: - Diagnostic bacteriology - Isolation of pathogens, diagnosis - Vaccine production - Industry
  • 3. 
    Detection of bacteria in a sample
  • 4. 
    Detection of bacteria in a sample Direct methods: - Smear - nasal, vaginal, pus, mastitic milk, blood - Impression smear - cut surface - PCR -> take out the gene and multiply it and check on gel Indirect methods: - Animal trial - Innoculate onto a medium
  • 5. 
    Composition of media for indirect method
  • 6. 
    Classification of media Demand of bacteria!
  • 7. 
    Agar for Indirect method Agar Sample may have more than one type of bacteria.
  • 8. 
    Use inoculating loops to put bacteria onto agar plate
  • 9. 
    If organ
  • 10. 
    Stab the inoculate loop though the burnt surface of the organ
  • 11. 
    Different ways to spread sample onto agar plate  
  • 12. 
    Gram Positive and Gram Negative bacteria Bacteria can be differentiated into Gram positive or Gram negative by testing them with the “Gram stain”.  The stain attaches to part of the bacterial cell wall called peptidoglycan and causes a purple colouration. Gram positive appear: blue to purple Structure: Have thick layer of peptidoglycan over inner cytoplasmic membrane. lack LPS-lipopolysaccharides Gram negative appear: pink to red Structure: In gram negative bacteria the peptidoglycan layer is thinner and is located between space of the outer and inner cytoplasmic membrane. cell wall contains LPS which make them virulent  
  • 13. 
    If media needs to be inoculated with bacteria then the media needs to fulfill the bacteria needs:
  • 14. 
    Examples of obligate AEROBE bacteria
  • 15. 
    Examples of obligate ANAEROBE bacteria
  • 16. 
    Examples of obligate FACULTATIVE ANAEROBE bacteria
  • 17. 
    Microaerophile bacteria Can't tolerate high O2 but can't grow w/o it What % O2 and CO2?
  • 18. 
    Capnophile bacteria Need extra CO2 What % CO2?
  • 19. 
    Tube Growth at different depths in semi-solid agar shows bacterial preference for aerobic, microaerophile, facultative anaerobe, anaerobe. 21% O2 at surface of media
  • 20. 
    How can we prepare anaerobe environment for the bacteria? Petri dishes into jar, light candle => O2 used up
  • 21. 
    Temperature Categories of bacteria based on temperature ranges at which they can grow.  
  • 22. 
    37oC body temperature, most bacteria, most incubators 42oC Campylobacter jejuni, Brachyspria hyodysenteriae 4oC cold enrichment 15-20oC bacteria isolated from fishes, reptiles
  • 23. 
    Time of incubation 1-2 days - most colonies form fast 2-3 days - several pathogens need more time eg. Brucella 1-3 weeks - tuberculosis 6-12 weeks - paratuberculosis (3 months!)  
  • 24. 
    Steralisation vs disinfection
  • 25. 
    Dry heat - Oxidation - Flaming, Incineration Wet heat - Coagulation - Autoclave:  112oC, 30 min 121oC, 15 min But prions aren't inactivated, they need to be autoclaved for longer time Moist heat: Spores can survive 3h of boiling Spore forming bacteria: - boiling, steaming - tyndallization (fraction steralisation) (Briefly boiling the item, which will kill bacterial cells, but not bacterial spores, allowing it to cool and allowing the spores to germinate, and then re-heating to kill the bacteria. This is usually done three times in total. This process is not particularly reliable, so is not often used today.) Other techniques: - Gas - irridation - UV-light - filtration - NOT REALLY STERALIZATION - pore size 0.22 um, bacteria w/o shape & virus can go through filter, microplasm doesn't have any shape and can go through filter
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