This quiz focuses on Staphylococcus, exploring its hemolytic properties and mannitol salt fermentation. It assesses understanding of bacterial behavior in clinical settings, differentiating species based on growth media reactions. Essential for students and professionals in microbiology and healthcare.
Red agglutination with a blue background
No agglutination
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Incomplete lysis of the RBCs (green tint to the media)
Complete lysis of the RBCs (complete clearing of the media)
No lysis of the RBCs (no hemolysis)
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Incomplete lysis of the RBCs (green tint to the media)
Complete lysis of the RBCs (complete clearing of the media)
No lysis of the RBCs (no hemolysis)
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- detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation. This is a key differential test for the identification of Staphylococcus aureus.
Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.
Key differential test for identification of S. saprophyticus Procedure
Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.
Detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation. This is a key differential test for the identification of Staphylococcus aureus.
Key differential test for identification of S. saprophyticus Procedure
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Detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation.
Key differential test for identification of S. saprophyticus
Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.
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Red agglutination with a blue background
No agglutination
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Coagulation of the plasma (clot formation). Note: any degree of coagulation is considered positive.
Yellow colony/yellow area around colony
Red agglutination with a blue background
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Red agglutination with a blue background
Yellow colony/yellow area around colony
No coagulation or clot formation
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This is a rapid latex slide agglutination test that utilizes protein-coated latex particles to detect coagulase (clumping factor) and/or Protein A that are characteristics associated with Staphylococcus aureus.
Key differential test for identification of S. saprophyticus
This is a key differential test for the identification of Staphylococcus aureus.
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No coagulation or clot formation
Red colony/no color change around colony
Yellow colony/yellow area around colon
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>= 17 mm Susceptible
Yellow colony/yellow area around colony
Red colony/no color change around colony
No coagulation or clot formation
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>= 17 mm Susceptible
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