Bacteria Of Clinical Significance: Part II Staphylococcus
Choose the best answer
- 1.Beta hemolysis
- A.
Incomplete lysis of the RBCs (green tint to the media)
- B.
Complete lysis of the RBCs (complete clearing of the media)
- C.
No lysis of the RBCs (no hemolysis)
- 2.Alpha hemolysis:
- A.
Incomplete lysis of the RBCs (green tint to the media)
- B.
Complete lysis of the RBCs (complete clearing of the media)
- C.
No lysis of the RBCs (no hemolysis)
- 3.Non-hemolytic:
- A.
Incomplete lysis of the RBCs (green tint to the media)
- B.
Complete lysis of the RBCs (complete clearing of the media)
- C.
No lysis of the RBCs (no hemolysis)
- 4.Mannitol salt fermentation:
- A.
Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.
- B.
Detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation. This is a key differential test for the identification of Staphylococcus aureus.
- C.
Key differential test for identification of S. saprophyticus Procedure
- 5.Positive
- A.
No coagulation or clot formation
- B.
Red colony/no color change around colony
- C.
Yellow colony/yellow area around colon
- 6.Negative:
- A.
Yellow colony/yellow area around colony
- B.
Red colony/no color change around colony
- C.
No coagulation or clot formation
- 7.Tube coagulase
- A.
- detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation. This is a key differential test for the identification of Staphylococcus aureus.
- B.
Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.
- C.
Key differential test for identification of S. saprophyticus Procedure
- 8.Positive:
- A.
Coagulation of the plasma (clot formation). Note: any degree of coagulation is considered positive.
- B.
Yellow colony/yellow area around colony
- C.
Red agglutination with a blue background
- 9.Negative:
- A.
Red agglutination with a blue background
- B.
Yellow colony/yellow area around colony
- C.
No coagulation or clot formation
- 10.Novobiocin inhibition test
- A.
Detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation.
- B.
Key differential test for identification of S. saprophyticus
- C.
Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.
- 11.Negative
- A.
>= 17 mm Susceptible
- B.
- 12.Positive
- A.
>= 17 mm Susceptible
- B.
- 13.Sure-Vue Color Staph Identification Test Kit
- A.
This is a rapid latex slide agglutination test that utilizes protein-coated latex particles to detect coagulase (clumping factor) and/or Protein A that are characteristics associated with Staphylococcus aureus.
- B.
Key differential test for identification of S. saprophyticus
- C.
This is a key differential test for the identification of Staphylococcus aureus.
- 14.Positive reaction:
- A.
Red agglutination with a blue background
- B.
No agglutination
- 15.Negative reaction:
- A.
Red agglutination with a blue background
- B.
No agglutination
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