Bacteria Of Clinical Significance: Part II Staphylococcus

15 Questions | Total Attempts: 56

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Questions and Answers
  • 1. 
    Beta hemolysis
    • A. 

      Incomplete lysis of the RBCs (green tint to the media)

    • B. 

      Complete lysis of the RBCs (complete clearing of the media)

    • C. 

      No lysis of the RBCs (no hemolysis)

  • 2. 
    Alpha hemolysis:
    • A. 

      Incomplete lysis of the RBCs (green tint to the media)

    • B. 

      Complete lysis of the RBCs (complete clearing of the media)

    • C. 

      No lysis of the RBCs (no hemolysis)

  • 3. 
    Non-hemolytic:
    • A. 

      Incomplete lysis of the RBCs (green tint to the media)

    • B. 

      Complete lysis of the RBCs (complete clearing of the media)

    • C. 

      No lysis of the RBCs (no hemolysis)

  • 4. 
    Mannitol salt fermentation:
    • A. 

      Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.

    • B. 

      Detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation. This is a key differential test for the identification of Staphylococcus aureus.

    • C. 

      Key differential test for identification of S. saprophyticus Procedure

  • 5. 
    Positive
    • A. 

      No coagulation or clot formation

    • B. 

      Red colony/no color change around colony

    • C. 

      Yellow colony/yellow area around colon

  • 6. 
    Negative:
    • A. 

      Yellow colony/yellow area around colony

    • B. 

      Red colony/no color change around colony

    • C. 

      No coagulation or clot formation

  • 7. 
    Tube coagulase
    • A. 

      - detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation. This is a key differential test for the identification of Staphylococcus aureus.

    • B. 

      Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.

    • C. 

      Key differential test for identification of S. saprophyticus Procedure

  • 8. 
    Positive:
    • A. 

      Coagulation of the plasma (clot formation). Note: any degree of coagulation is considered positive.

    • B. 

      Yellow colony/yellow area around colony

    • C. 

      Red agglutination with a blue background

  • 9. 
    Negative:
    • A. 

      Red agglutination with a blue background

    • B. 

      Yellow colony/yellow area around colony

    • C. 

      No coagulation or clot formation

  • 10. 
    Novobiocin inhibition test
    • A. 

      Detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation.

    • B. 

      Key differential test for identification of S. saprophyticus

    • C. 

      Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.

  • 11. 
    Negative
    • A. 

      >= 17 mm Susceptible

    • B. 

  • 12. 
    Positive
    • A. 

      >= 17 mm Susceptible

    • B. 

  • 13. 
    Sure-Vue Color Staph Identification Test Kit
    • A. 

      This is a rapid latex slide agglutination test that utilizes protein-coated latex particles to detect coagulase (clumping factor) and/or Protein A that are characteristics associated with Staphylococcus aureus.

    • B. 

      Key differential test for identification of S. saprophyticus

    • C. 

      This is a key differential test for the identification of Staphylococcus aureus.

  • 14. 
    Positive reaction:
    • A. 

      Red agglutination with a blue background

    • B. 

      No agglutination

  • 15. 
    Negative reaction:
    • A. 

      Red agglutination with a blue background

    • B. 

      No agglutination

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