Bacteria Of Clinical Significance: Part II Staphylococcus

15 Questions | By Maer | Updated: Jan 18, 2023 | Attempts: 107

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Bacteria Of Clinical Significance: Part II Staphylococcus - Quiz

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1.

Beta hemolysis
- A.
  
  Incomplete lysis of the RBCs (green tint to the media)
- B.
  
  Complete lysis of the RBCs (complete clearing of the media)
- C.
  
  No lysis of the RBCs (no hemolysis)
2.

Alpha hemolysis:
- A.
  
  Incomplete lysis of the RBCs (green tint to the media)
- B.
  
  Complete lysis of the RBCs (complete clearing of the media)
- C.
  
  No lysis of the RBCs (no hemolysis)
3.

Non-hemolytic:
- A.
  
  Incomplete lysis of the RBCs (green tint to the media)
- B.
  
  Complete lysis of the RBCs (complete clearing of the media)
- C.
  
  No lysis of the RBCs (no hemolysis)
4.

Mannitol salt fermentation:
- A.
  
  Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.
- B.
  
  Detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation. This is a key differential test for the identification of Staphylococcus aureus.
- C.
  
  Key differential test for identification of S. saprophyticus Procedure
5.

Positive
- A.
  
  No coagulation or clot formation
- B.
  
  Red colony/no color change around colony
- C.
  
  Yellow colony/yellow area around colon
6.

Negative:
- A.
  
  Yellow colony/yellow area around colony
- B.
  
  Red colony/no color change around colony
- C.
  
  No coagulation or clot formation
7.

Tube coagulase
- A.
  
  - detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation. This is a key differential test for the identification of Staphylococcus aureus.
- B.
  
  Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.
- C.
  
  Key differential test for identification of S. saprophyticus Procedure
8.

Positive:
- A.
  
  Coagulation of the plasma (clot formation). Note: any degree of coagulation is considered positive.
- B.
  
  Yellow colony/yellow area around colony
- C.
  
  Red agglutination with a blue background
9.

Negative:
- A.
  
  Red agglutination with a blue background
- B.
  
  Yellow colony/yellow area around colony
- C.
  
  No coagulation or clot formation
10.

Novobiocin inhibition test
- A.
  
  Detects free coagulase which is an extracellular protein excreted by the organism that causes fibrin clot formation.
- B.
  
  Key differential test for identification of S. saprophyticus
- C.
  
  Mannitol Salt Agar is a selective and differential media. It selects for the growth of Staphylococcus sp. while it inhibits the growth of other organisms due to its elevated salt concentration (7.5% NaCl). It differentiates Staphylococcus sp. based on mannitol fermentation.
11.

Negative
- A.
  
  >= 17 mm Susceptible
- B.
12.

Positive
- A.
  
  >= 17 mm Susceptible
- B.
13.

Sure-Vue Color Staph Identification Test Kit
- A.
  
  This is a rapid latex slide agglutination test that utilizes protein-coated latex particles to detect coagulase (clumping factor) and/or Protein A that are characteristics associated with Staphylococcus aureus.
- B.
  
  Key differential test for identification of S. saprophyticus
- C.
  
  This is a key differential test for the identification of Staphylococcus aureus.
14.

Positive reaction:
- A.
  
  Red agglutination with a blue background
- B.
  
  No agglutination
15.

Negative reaction:
- A.
  
  Red agglutination with a blue background
- B.
  
  No agglutination