Quiz: Selection, Screening, And Analysis Of Recombinant DNA

50 Questions | By Faunsoed | Last updated: Mar 22, 2022 | Total Attempts: 319

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Quiz: Selection, Screening, And Analysis Of Recombinant DNA - Quiz

The study of human DNA has led to a lot of medical breakthroughs and did you know that science has advanced so much that one can actually create DNA molecules in the laboratory and even be combined to form a new genetic sequence? The quiz below is designed to test out just how much you know about selection, screening, and analysis when it comes to recombinant DNA.

Questions and Answers

1.

Success in any cloning experiment depends on being able to identify the desired gene sequence among the many different recombinants that may be produced.
- A.
  
  TRUE
- B.
  
  FALSE
2.

Selection is where some sort of pressure (e.g. the presence of an antibiotic) is applied during the growth of host cells containing recombinant DNA.
- A.
  
  TRUE
- B.
  
  FALSE
3.

Screening is a procedure by which a population of viable cells is subjected to some sort of analysis that enables the desired sequences to be identified.
- A.
  
  TRUE
- B.
  
  FALSE
4.

Antibiotic resistance marker genes provide a simple and reliable way to select for the presence of vectors in cells.
- A.
  
  TRUE
- B.
  
  FALSE
5.

Genetic selection and screening methods rely on the expression (or non-expression) of certain traits.
- A.
  
  TRUE
- B.
  
  FALSE
6.

One of the simplest genetic selection methods involves the use of antibiotics to select for the presence of vector molecules.
- A.
  
  TRUE
- B.
  
  FALSE
7.

The plasmid pBR322 contains genes for ampicillin resistance (Ap^r) and tetracycline resistance (Tc^r).
- A.
  
  TRUE
- B.
  
  FALSE
8.

The presence of the plasmid pBR322 in cells can be detected by plating potential transformants on an agar medium that contains either (or both) of ampicillin and tetracycline antibiotics.
- A.
  
  TRUE
- B.
  
  FALSE
9.

The X-gal detection system can be used where a functional β-galactosidase gene is present in the host/vector system.
- A.
  
  TRUE
- B.
  
  FALSE
10.

The presence of cloned DNA fragments can be detected if the insert interrupts the coding sequence of a gene.
- A.
  
  TRUE
- B.
  
  FALSE
11.

Origin of replication can be used to identify a vector carrying a cloned insert.
- A.
  
  TRUE
- B.
  
  FALSE
12.

Antibiotic resistance can not be used as an insertional inactivation system if DNA fragments are cloned into a restriction site within an anitbiotic-resistance gene.
- A.
  
  TRUE
- B.
  
  FALSE
13.

Plaque morphology can not also be used as a screening method for certain λ vectors such as λgt10, which contain the cI gene.
- A.
  
  TRUE
- B.
  
  FALSE
14.

The LacZ gene encodes the cI repressor, which is responsible for the formation of lysogens
- A.
  
  TRUE
- B.
  
  FALSE
15.

Direct complementation of a defined mutation may be feasible if the gene system is unknown and an appropriate mutant is available.
- A.
  
  TRUE
- B.
  
  FALSE
16.

The antiparalel nature of DNA strands means that a sequence-specific probe is a very powerful screening tool.
- A.
  
  TRUE
- B.
  
  FALSE
17.

Nucleic acid hybridisation is a very powerful method to screen clone banks and is one of the key techniques to amplify a gene.
- A.
  
  TRUE
- B.
  
  FALSE
18.

The production of a cDNA or genomic DNA library is often termed the ‘blotting’ approach, as a large number of essentially random recombinants is generated.
- A.
  
  TRUE
- B.
  
  FALSE
19.

By using a defined nucleic acid probe, a cDNA or genomic DNA library can be cleaved and the clone(s) of interest identified.
- A.
  
  TRUE
- B.
  
  FALSE
20.

Homologous or heterologous probes may be used in screening protocols, if genes have sequences similar enough to enable hybridisation to be unstable under relatively high stringency conditions.
- A.
  
  TRUE
- B.
  
  FALSE
21.

The power of nucleic acid hybridisation lies in the fact that complementary sequences will bind to each other with a very high degree of fidelity.
- A.
  
  TRUE
- B.
  
  FALSE
22.

There are three main types of DNA probe: (1) cDNA, (2) genomic DNA, and (3) oligonucleotides.
- A.
  
  TRUE
- B.
  
  FALSE
23.

The availability of a particular probe will depend on what is known about the target gene sequence.
- A.
  
  TRUE
- B.
  
  FALSE
24.

If a cDNA clone has already been obtained and identified, the cDNA can be used to screen a genomic library and isolate the gene sequence itself.
- A.
  
  TRUE
- B.
  
  FALSE
25.

Genomic DNA probes are usually fragments of cloned sequences that are used either as heterologous probes or to identify other clones that contain additional parts of the gene in question.
- A.
  
  TRUE
- B.
  
  FALSE