Quiz: Selection, Screening, And Analysis Of Recombinant DNA

50 Questions | Total Attempts: 245

SettingsSettingsSettings
Please wait...
Quiz: Selection, Screening, And Analysis Of Recombinant DNA

The study of human DNA has led to a lot of medical breakthroughs and did you know that science has advanced so much that one can actually create DNA molecules in the laboratory and even be combined to form a new genetic sequence? The quiz below is designed to test out just how much you know about selection, screening, and analysis when it comes to recombinant DNA.


Questions and Answers
  • 1. 
    Success in any cloning experiment depends on being able to identify the desired gene sequence among the many different recombinants that may be produced.
    • A. 

      TRUE

    • B. 

      FALSE

  • 2. 
    Selection is where some sort of pressure (e.g. the presence of an antibiotic) is applied during the growth of host cells containing recombinant DNA.
    • A. 

      TRUE

    • B. 

      FALSE

  • 3. 
    Screening is a procedure by which a population of viable cells is subjected to some sort of analysis that enables the desired sequences to be identified.
    • A. 

      TRUE

    • B. 

      FALSE

  • 4. 
    Antibiotic resistance marker genes provide a simple and reliable way to select for the presence of vectors in cells.
    • A. 

      TRUE

    • B. 

      FALSE

  • 5. 
    Genetic selection and screening methods rely on the expression (or non-expression) of certain traits.
    • A. 

      TRUE

    • B. 

      FALSE

  • 6. 
    One of the simplest genetic selection methods involves the use of antibiotics to select for the presence of vector molecules.
    • A. 

      TRUE

    • B. 

      FALSE

  • 7. 
    The plasmid pBR322 contains genes for ampicillin resistance (Apr) and tetracycline resistance (Tcr).
    • A. 

      TRUE

    • B. 

      FALSE

  • 8. 
    The presence of the plasmid pBR322 in cells can be detected by plating potential transformants on an agar medium that contains either (or both) of ampicillin and tetracycline antibiotics.
    • A. 

      TRUE

    • B. 

      FALSE

  • 9. 
    The X-gal detection system can be used where a functional β-galactosidase gene is present in the host/vector system.
    • A. 

      TRUE

    • B. 

      FALSE

  • 10. 
    The presence of cloned DNA fragments can be detected if the insert interrupts the coding sequence of a gene.
    • A. 

      TRUE

    • B. 

      FALSE

  • 11. 
    Origin of replication can be used to identify a vector carrying a cloned insert.
    • A. 

      TRUE

    • B. 

      FALSE

  • 12. 
    Antibiotic resistance can not be used as an insertional inactivation system if DNA fragments are cloned into a restriction site within an anitbiotic-resistance gene.
    • A. 

      TRUE

    • B. 

      FALSE

  • 13. 
    Plaque morphology can not also be used as a screening method for certain λ vectors such as λgt10, which contain the cI gene.
    • A. 

      TRUE

    • B. 

      FALSE

  • 14. 
    The LacZ gene encodes the cI repressor, which is responsible for the formation of lysogens
    • A. 

      TRUE

    • B. 

      FALSE

  • 15. 
    Direct complementation of a defined mutation may be feasible if the gene system is unknown and an appropriate mutant is available.
    • A. 

      TRUE

    • B. 

      FALSE

  • 16. 
    The antiparalel nature of DNA strands means that a sequence-specific probe is a very powerful screening tool.
    • A. 

      TRUE

    • B. 

      FALSE

  • 17. 
    Nucleic acid hybridisation is a very powerful method to screen clone banks and is one of the key techniques to amplify a gene.
    • A. 

      TRUE

    • B. 

      FALSE

  • 18. 
    The production of a cDNA or genomic DNA library is often termed the ‘blotting’ approach, as a large number of essentially random recombinants is generated.
    • A. 

      TRUE

    • B. 

      FALSE

  • 19. 
    By using a defined nucleic acid probe, a cDNA or genomic DNA library can be cleaved and the clone(s) of interest identified.
    • A. 

      TRUE

    • B. 

      FALSE

  • 20. 
    Homologous or heterologous probes may be used in screening protocols, if genes have sequences similar enough to enable hybridisation to be unstable under relatively high stringency conditions.
    • A. 

      TRUE

    • B. 

      FALSE

  • 21. 
    The power of nucleic acid hybridisation lies in the fact that complementary sequences will bind to each other with a very high degree of fidelity.
    • A. 

      TRUE

    • B. 

      FALSE

  • 22. 
    There are three main types of DNA probe: (1) cDNA, (2) genomic DNA, and (3) oligonucleotides.
    • A. 

      TRUE

    • B. 

      FALSE

  • 23. 
    The availability of a particular probe will depend on what is known about the target gene sequence.
    • A. 

      TRUE

    • B. 

      FALSE

  • 24. 
    If a cDNA clone has already been obtained and identified, the cDNA can be used to screen a genomic library and isolate the gene sequence itself.
    • A. 

      TRUE

    • B. 

      FALSE

  • 25. 
    Genomic DNA probes are usually fragments of cloned sequences that are used either as heterologous probes or to identify other clones that contain additional parts of the gene in question.
    • A. 

      TRUE

    • B. 

      FALSE

Back to Top Back to top