Quiz: Selection, Screening, And Analysis Of Recombinant DNA
50 Questions
| Total Attempts: 245
(250).jpg "Quiz: Selection, Screening, And Analysis Of Recombinant DNA")
The study of human DNA has led to a lot of medical breakthroughs and did you know that science has advanced so much that one can actually create DNA molecules in the laboratory and even be combined to form a new genetic sequence? The quiz below is designed to test out just how much you know about selection, screening, and analysis when it comes to recombinant DNA.
Questions and Answers
- 1.Success in any cloning experiment depends on being able to identify the desired gene sequence among the many different recombinants that may be produced.
- A.
TRUE
- B.
FALSE
- 2.Selection is where some sort of pressure (e.g. the presence of an antibiotic) is applied during the growth of host cells containing recombinant DNA.
- A.
TRUE
- B.
FALSE
- 3.Screening is a procedure by which a population of viable cells is subjected to some sort of analysis that enables the desired sequences to be identified.
- A.
TRUE
- B.
FALSE
- 4.Antibiotic resistance marker genes provide a simple and reliable way to select for the presence of vectors in cells.
- A.
TRUE
- B.
FALSE
- 5.Genetic selection and screening methods rely on the expression (or non-expression) of certain traits.
- A.
TRUE
- B.
FALSE
- 6.One of the simplest genetic selection methods involves the use of antibiotics to select for the presence of vector molecules.
- A.
TRUE
- B.
FALSE
- 7.The plasmid pBR322 contains genes for ampicillin resistance (Apr) and tetracycline resistance (Tcr).
- A.
TRUE
- B.
FALSE
- 8.The presence of the plasmid pBR322 in cells can be detected by plating potential transformants on an agar medium that contains either (or both) of ampicillin and tetracycline antibiotics.
- A.
TRUE
- B.
FALSE
- 9.The X-gal detection system can be used where a functional β-galactosidase gene is present in the host/vector system.
- A.
TRUE
- B.
FALSE
- 10.The presence of cloned DNA fragments can be detected if the insert interrupts the coding sequence of a gene.
- A.
TRUE
- B.
FALSE
- 11.Origin of replication can be used to identify a vector carrying a cloned insert.
- A.
TRUE
- B.
FALSE
- 12.Antibiotic resistance can not be used as an insertional inactivation system if DNA fragments are cloned into a restriction site within an anitbiotic-resistance gene.
- A.
TRUE
- B.
FALSE
- 13.Plaque morphology can not also be used as a screening method for certain λ vectors such as λgt10, which contain the cI gene.
- A.
TRUE
- B.
FALSE
- 14.The LacZ gene encodes the cI repressor, which is responsible for the formation of lysogens
- A.
TRUE
- B.
FALSE
- 15.Direct complementation of a defined mutation may be feasible if the gene system is unknown and an appropriate mutant is available.
- A.
TRUE
- B.
FALSE
- 16.The antiparalel nature of DNA strands means that a sequence-specific probe is a very powerful screening tool.
- A.
TRUE
- B.
FALSE
- 17.Nucleic acid hybridisation is a very powerful method to screen clone banks and is one of the key techniques to amplify a gene.
- A.
TRUE
- B.
FALSE
- 18.The production of a cDNA or genomic DNA library is often termed the ‘blotting’ approach, as a large number of essentially random recombinants is generated.
- A.
TRUE
- B.
FALSE
- 19.By using a defined nucleic acid probe, a cDNA or genomic DNA library can be cleaved and the clone(s) of interest identified.
- A.
TRUE
- B.
FALSE
- 20.Homologous or heterologous probes may be used in screening protocols, if genes have sequences similar enough to enable hybridisation to be unstable under relatively high stringency conditions.
- A.
TRUE
- B.
FALSE
- 21.The power of nucleic acid hybridisation lies in the fact that complementary sequences will bind to each other with a very high degree of fidelity.
- A.
TRUE
- B.
FALSE
- 22.There are three main types of DNA probe: (1) cDNA, (2) genomic DNA, and (3) oligonucleotides.
- A.
TRUE
- B.
FALSE
- 23.The availability of a particular probe will depend on what is known about the target gene sequence.
- A.
TRUE
- B.
FALSE
- 24.If a cDNA clone has already been obtained and identified, the cDNA can be used to screen a genomic library and isolate the gene sequence itself.
- A.
TRUE
- B.
FALSE
- 25.Genomic DNA probes are usually fragments of cloned sequences that are used either as heterologous probes or to identify other clones that contain additional parts of the gene in question.
- A.
TRUE
- B.
FALSE
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