Microbiology: Mycobateria And Other Bacteria With Unusual Growth Requirements

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Microbiology: Mycobateria And Other Bacteria With Unusual Growth Requirements - Quiz

This is a 50 item quiz. Time limit: 1 hour.


Questions and Answers
  • 1. 
    Runyon's classification of NTM are based on:
    • A. 

      Colony texture

    • B. 

      Colonial pigmentation

    • C. 

      Epidemiology

    • D. 

      Rate of growth

    • E. 

      B and D

  • 2. 
    Reservoir of M. kansasii
    • A. 

      Humans with cavitary diseases

    • B. 

      Tap water

    • C. 

      Children

    • D. 

      Natural waters

    • E. 

      None of the above

  • 3. 
    Which does NOT develop pigment following exposure to light after being grown in the dark for more than 7 days?
    • A. 

      M. asiaticum

    • B. 

      M. kansasii

    • C. 

      M. szulgai

    • D. 

      A and B

    • E. 

      All of the above

  • 4. 
    VeHabitat of M. scrofulaceum
    • A. 

      Soil

    • B. 

      Water

    • C. 

      Raw milk

    • D. 

      Dairy products

    • E. 

      All of the above

  • 5. 
    Which is NOT true
    • A. 

      M. gordonae is nonpathogenic

    • B. 

      Mycobacteria are slow growers

    • C. 

      Mycobacteria are nonpigmented

    • D. 

      Mycobacteria contain N-acteylmuramic acid in their cell wall

    • E. 

      Tuberculin testing is the primary means of identifying individuals with M. tuberculosis

  • 6. 
    Which is does NOT belong to the Mycobacterium avium complex?
    • A. 

      M. avium

    • B. 

      M. lepraemurium

    • C. 

      M. silvaticum

    • D. 

      M. leprae

    • E. 

      M. intracellure

  • 7. 
    Which is NOT true?
    • A. 

      Translucent or tranparent colonies of MAC are more virulent than opaque colonies

    • B. 

      M. leprae cannot be cultivated in vitro

    • C. 

      Numerous AFB can be obtained from lesions of Tuberculoid leprosy

    • D. 

      Wade-Fite technique is used for acid fast staining of paraffinized tissue

    • E. 

      M. marinum grows at RT

  • 8. 
    Specimen of choice for NTM
    • A. 

      Sputum

    • B. 

      Gastric aspirate

    • C. 

      Urine

    • D. 

      Fecal specimen

    • E. 

      Bronchial washings

  • 9. 
    Which is not a desirablespecimen for mycobacteria?
    • A. 

      Gastric aspirate

    • B. 

      Blood

    • C. 

      CSF

    • D. 

      24 hr urine

    • E. 

      Semen

  • 10. 
    Specimen of choice for MAC in AIDS patients
    • A. 

      Blood

    • B. 

      CSF

    • C. 

      Stool

    • D. 

      Sputum

    • E. 

      A and D

  • 11. 
    Which is a mucolytic agent in decontamination and concentration?
    • A. 

      N-Acetyl-L-Cystein

    • B. 

      NaOH

    • C. 

      Zerphiran Chloride

    • D. 

      Trisodium Phosphate

    • E. 

      A and B

  • 12. 
    A milder decontaminating solution allowing longer time period for decontamination
    • A. 

      NALC-NaOH method

    • B. 

      Benzalkonium Chloride-Trisodium Phosphate Method

    • C. 

      Modfied Benzalkonium Chloride-Trisodium Phosphate Method

    • D. 

      All of the above

    • E. 

      A and B

  • 13. 
    Which is NOT true?
    • A. 

      For culture, use 2 solid media and 1 liquid media

    • B. 

      Voided urine does not require decontamination

    • C. 

      Bottle caps should be loose in the first 2 weeks of incubation

    • D. 

      NaOH is a toxic decontaminating agent

    • E. 

      B and D

  • 14. 
    Excellent medium for MOTT especially MAC
    • A. 

      Lowenstein-Jensen with pyruic acid

    • B. 

      L-J with iron

    • C. 

      Bactec 12B medium

    • D. 

      Mitchison's selective 7H 11

    • E. 

      Wallenstein

  • 15. 
    Which is NOT correctly matched?
    • A. 

      M. tuberculosis - tapered ends

    • B. 

      MOTT - thicker and more beaded

    • C. 

      M. avium-intracellulare - short and coccobacillary

    • D. 

      M. kansasii - barber's pole appearance

    • E. 

      None of the above

  • 16. 
    A buff colored, slow growing, rough colony, and a positive niacin test is a preliminary ID for:
    • A. 

      M. tuberculosis

    • B. 

      M. bovis

    • C. 

      M. kansasii

    • D. 

      MAC

    • E. 

      MOTT

  • 17. 
    Which does NOT reduce nitrate?
    • A. 

      M. tuberculosis

    • B. 

      M. scrofulaceum

    • C. 

      M. szulgai

    • D. 

      M. fortuitum

    • E. 

      M. kansasii

  • 18. 
    Heating at 68 degress Celsius for 20min inactivates catalase in:
    • A. 

      M. bovis

    • B. 

      M. tuberculosis

    • C. 

      M. gastri

    • D. 

      M. haemophlium

    • E. 

      All of the above

  • 19. 
    Which are rapid growers?
    • A. 

      M. fortuitum

    • B. 

      M. gordonae

    • C. 

      M. chelonae

    • D. 

      B and C

    • E. 

      A and C

  • 20. 
    Inhibited by thiophene-2-carboxylic acid hydrazide
    • A. 

      M. marinum

    • B. 

      M. bovis

    • C. 

      M. tuberculosis

    • D. 

      M. asiaticum

    • E. 

      None of the above

  • 21. 
    Chlamydia are:
    • A. 

      Cell wall deficient

    • B. 

      Produce endotoxin and exotoxin

    • C. 

      Obligate intracellular parasites

    • D. 

      All of the above

    • E. 

      Only A and C

  • 22. 
    Has pear shaped elemental bodies
    • A. 

      C. psittaci

    • B. 

      C. trachomatis

    • C. 

      C. pneumoniae

    • D. 

      All of the above

    • E. 

      A and B

  • 23. 
    Which is NOT true?
    • A. 

      C. trachomatis causes trachoma

    • B. 

      C. trachomatis has 18 species specific Ag while C. psittaci has 15

    • C. 

      Chlamydia has tropism for columnar cells

    • D. 

      Inclusion conjunctivitis is caused by C. trachomatis

    • E. 

      Lymphogranuloma venereum is caused by C. psittaci

  • 24. 
    Cell lines used in culture for C. trachomatis
    • A. 

      HL cell line

    • B. 

      HeLa cells

    • C. 

      Hep 2 cells

    • D. 

      McCoy cells

    • E. 

      B and D

  • 25. 
    Levinthal-Cole-Lillie bodies are inclusions in
    • A. 

      C. trachomatis

    • B. 

      C. psittaci

    • C. 

      C. pneumoniae

    • D. 

      All of the above

    • E. 

      None of the above

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