Microscopy Principle And Application
- 1.What is the minimum distance for the eye to focus any object?
- A.
A) 11 cm
- B.
B) 25 cm
- C.
C) 32 cm
- D.
D) 4 2 cm
- 2.Resolving power of a microscope is a function of____________
- A.
A) Wavelength of light used
- B.
B) Numerical aperture of lens system
- C.
C) Refractive index
- D.
D) Wavelength of light used and numerical aperture of lens system
- 3.Oil immersion objective lens has an NA value of____________
- A.
A) 0.65
- B.
B) 0.85
- C.
C) 1.33
- D.
D) 1.00
- 4.In Phase contrast microscopy, the rate at which light enters through objects is__________
- A.
A) Constant
- B.
B) Inversely proportional to their refractive indices
- C.
C) Directly proportional to their refractive indices
- D.
D) Exponentially related to their refractive indices
- 5.Which of the following is used in electron microscope?
- A.
A) electron beams
- B.
B) magnetic fields
- C.
C) light waves
- D.
D) electron beams and magnetic fields
- 6.Electron Microscope can give a magnification up to ___________
- A.
A) 400,000X
- B.
B) 100,000X
- C.
C) 15000X
- D.
D) 100X
- 7.Which of the following are true for electron microscopy?
- A.
A) specimen should be thin and dry
- B.
B) image is obtained on a phosphorescent screen
- C.
C) electron beam must pass through evacuated chamber
- D.
D) specimen should be thin and dry, image is obtained on a phosphorescent screen and electron beam must pass through evacuated chambe
- 8.Negative Staining is used for examining _____________
- A.
A) virus particles
- B.
B) protein molecules
- C.
C) bacterial flagella
- D.
D) virus particles, protein molecules and bacterial flagella
- 9.Where do we obtain the magnified image of the specimen in SEM?
- A.
A) cathode ray tube
- B.
B) phosphorescent screen
- C.
C) anode
- D.
D) scanning generator
- 10.Which of the following techniques are used in Transmission Electron Microscopy (TEM) for examining cellular structure?
- A.
A) Negative-Staining
- B.
B) Shadow Casting
- C.
C) Ultrathin Sectioning
- D.
D) Negative-Staining, Shadow Casting, Ultrathin Sectioning, Freeze-Etching
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