Microscopy Principle And Application

10 Questions | Total Attempts: 88

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Microscopy Principle And Application


Questions and Answers
  • 1. 
     What is the minimum distance for the eye to focus any object?
    • A. 

      A) 11 cm

    • B. 

      B) 25 cm

    • C. 

      C) 32 cm

    • D. 

      D) 4 2 cm

  • 2. 
    Resolving power of a microscope is a function of____________
    • A. 

      A) Wavelength of light used

    • B. 

      B) Numerical aperture of lens system

    • C. 

      C) Refractive index

    • D. 

      D) Wavelength of light used and numerical aperture of lens system

  • 3. 
    Oil immersion objective lens has an NA value of____________
    • A. 

      A) 0.65

    • B. 

      B) 0.85

    • C. 

      C) 1.33

    • D. 

      D) 1.00

  • 4. 
    In Phase contrast microscopy, the rate at which light enters through objects is__________
    • A. 

      A) Constant

    • B. 

      B) Inversely proportional to their refractive indices

    • C. 

      C) Directly proportional to their refractive indices

    • D. 

      D) Exponentially related to their refractive indices

  • 5. 
    Which of the following is used in electron microscope?
    • A. 

      A) electron beams

    • B. 

      B) magnetic fields

    • C. 

      C) light waves

    • D. 

      D) electron beams and magnetic fields

  • 6. 
     Electron Microscope can give a magnification up to ___________
    • A. 

      A) 400,000X

    • B. 

      B) 100,000X

    • C. 

      C) 15000X

    • D. 

      D) 100X

  • 7. 
    Which of the following are true for electron microscopy?
    • A. 

      A) specimen should be thin and dry

    • B. 

      B) image is obtained on a phosphorescent screen

    • C. 

      C) electron beam must pass through evacuated chamber

    • D. 

      D) specimen should be thin and dry, image is obtained on a phosphorescent screen and electron beam must pass through evacuated chambe

  • 8. 
    Negative Staining is used for examining _____________
    • A. 

      A) virus particles

    • B. 

      B) protein molecules

    • C. 

      C) bacterial flagella

    • D. 

      D) virus particles, protein molecules and bacterial flagella

  • 9. 
    Where do we obtain the magnified image of the specimen in SEM?
    • A. 

      A) cathode ray tube

    • B. 

      B) phosphorescent screen

    • C. 

      C) anode

    • D. 

      D) scanning generator

  • 10. 
    Which of the following techniques are used in Transmission Electron Microscopy (TEM) for examining cellular structure?
    • A. 

      A) Negative-Staining

    • B. 

      B) Shadow Casting

    • C. 

      C) Ultrathin Sectioning

    • D. 

      D) Negative-Staining, Shadow Casting, Ultrathin Sectioning, Freeze-Etching

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