Light Microscope Training Practical
Welcome to the electronic science frontier classroom of the 21st century. This instrument will test your knowledge of component parts of a compound light microscope. Microscopes are tools that extend human vision by making enlarged images of objects.
When a "fill-in" type question presents itself in this test, be sure to read any directions. Please enter your answer(s) using all lower case letters.
I wish you good luck in your learning of the compound light microscope.
Questions and Answers
- 1.
What is the name of number 1?
- A.
Body Tube
- B.
Eyepiece
- C.
Stage
- D.
Light Source
- E.
Coarse Adjustment Knob
Correct Answer
B. EyepieceExplanation
The eyepiece is the part of a microscope that you look through to observe the specimen. It is located at the top of the body tube and is responsible for magnifying the image produced by the objective lens. The eyepiece typically contains a lens or a set of lenses that further magnify the image, allowing for detailed examination of the specimen.Rate this question:
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- 2.
What is the name of number 2?
- A.
Body Tube
- B.
Eyepiece
- C.
Revolving Nose piece
- D.
Base
- E.
Coarse Adjustment Knob
Correct Answer
C. Revolving Nose pieceExplanation
The correct answer is Revolving Nose piece. The revolving nosepiece is a part of a microscope that holds multiple objective lenses. It allows the user to easily switch between different magnifications by rotating the nosepiece. This is an essential component in microscopy as it enables the user to adjust the level of magnification required for their observations.Rate this question:
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- 3.
What is the name of number 3?
- A.
Body Tube
- B.
Microscope Stage Clips
- C.
Revolving Nose piece
- D.
Base
- E.
Objective Lenses
Correct Answer
E. Objective LensesExplanation
Objective lenses are the correct answer because they are the part of a microscope that is responsible for magnifying the specimen being viewed. They are located on the revolving nosepiece and can be switched out to provide different levels of magnification. The objective lenses play a crucial role in the overall functioning of the microscope by allowing the viewer to see the specimen in greater detail.Rate this question:
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- 4.
What is the name of number 4?
- A.
Power Switch
- B.
Microscope Stage Clips
- C.
Revolving Nose piece
- D.
Base
- E.
Stage
Correct Answer
E. StageExplanation
The given options are unrelated to the question, as they are all parts of a microscope. The correct answer is "Stage," which is the platform on which objects are placed for examination under a microscope.Rate this question:
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- 5.
What is the name of number 2 on this compound light microscope?
- A.
Fine Adjustment Knob
- B.
Revolving Nosepiece
- C.
Microscope Slide Clip
- D.
Body Tube
- E.
Ocular (eyepiece)
Correct Answer
D. Body TubeExplanation
The body tube is the correct answer because it refers to the part of a compound light microscope that connects the eyepiece to the objective lenses. It holds the ocular (eyepiece) and allows for the adjustment of the interpupillary distance. The body tube plays a crucial role in focusing the microscope and maintaining the correct distance between the eyepiece and the objective lenses.Rate this question:
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- 6.
What is the name of number 4 on this compound light microscope?
- A.
Scanning & High Power Objectives
- B.
Low Power Objective Lens
- C.
Microscope Slide Clip
- D.
Scanning & Low Power Objectives
- E.
Electric Switch
Correct Answer
D. Scanning & Low Power ObjectivesExplanation
The name of number 4 on this compound light microscope is Scanning & Low Power Objectives.Rate this question:
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- 7.
What is the name of number 11 on this compound light microscope?
- A.
Scanning Objective Lens
- B.
Low Power Objective Lens
- C.
Arm
- D.
High Power Objective Lens
- E.
Ocular (eyepiece)
Correct Answer
C. ArmExplanation
The arm is the correct answer because it is the part of the compound light microscope that connects the base to the head and provides support and stability to the entire microscope. It is usually curved and acts as a handle for carrying the microscope.Rate this question:
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- 8.
What is the name of number 12 on this compound light microscope?
- A.
Scanning Objective Lens
- B.
Low Power Objective Lens
- C.
Light Source
- D.
High Power Objective Lens
- E.
Stage Stop Screw
Correct Answer
C. Light SourceExplanation
The correct answer is "Light Source" because the question is asking for the name of number 12 on the compound light microscope, and the option "Light Source" corresponds to number 12 in the given list.Rate this question:
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- 9.
What is the name of number 6 on this compound light microscope?
- A.
Base
- B.
Stage Clips
- C.
Light Source
- D.
High Power Objective Lens
- E.
Eyepiece (Ocular)
Correct Answer
B. Stage ClipsExplanation
Stage clips are the name of number 6 on this compound light microscope. Stage clips are used to hold the slide in place on the stage of the microscope, ensuring that it remains steady and in the correct position for observation. They are typically located on either side of the stage and can be tightened or loosened to secure the slide.Rate this question:
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- 10.
What is the name of number 10 on this compound light microscope?
- A.
Fine Adjustment Knob
- B.
Stage Clips
- C.
Coarse Adjustment Knob
- D.
High Power Objective Lens
- E.
Stage Stop Screw
Correct Answer
C. Coarse Adjustment KnobExplanation
The correct answer is the Coarse Adjustment Knob. The Coarse Adjustment Knob is used to make large adjustments to the focus of the microscope. It moves the stage up and down to bring the specimen into approximate focus. This knob is typically larger and easier to manipulate than the Fine Adjustment Knob, which is used for fine-tuning the focus. The Stage Clips are used to hold the specimen in place on the stage. The High Power Objective Lens is used for high magnification, and the Stage Stop Screw limits the movement of the stage.Rate this question:
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- 11.
What is the name of number 8 on this compound light microscope?
- A.
Fine Adjustment Knob
- B.
Power Switch
- C.
Coarse Adjustment Knob
- D.
Automatic Focus Switch
- E.
Stage Stop Screw
Correct Answer
B. Power SwitchExplanation
The name of number 8 on this compound light microscope is the Power Switch. This switch is responsible for turning the microscope on and off, controlling the power supply to the instrument. It allows the user to easily start and stop the microscope, providing a convenient way to conserve energy and ensure proper functioning of the device.Rate this question:
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- 12.
What is the name of number 9 on this compound light microscope?
- A.
Fine Adjustment Knob
- B.
Power Switch
- C.
Coarse Adjustment Knob
- D.
Automatic Focus Switch
- E.
Stage Stop Screw
Correct Answer
A. Fine Adjustment KnobExplanation
The fine adjustment knob is the name of number 9 on this compound light microscope. It is used to make small, precise adjustments to the focus of the microscope. This knob allows the user to bring the specimen into sharp focus by moving the stage slightly up or down.Rate this question:
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- 13.
Number 12 is the power switch for this compound light microscope.
- A.
True
- B.
False
Correct Answer
B. False -
- 14.
Number 5 is the diaphragm for this compound light microscope.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
The diaphragm is a component of a compound light microscope that controls the amount of light passing through the specimen. By adjusting the diaphragm, the user can regulate the brightness and contrast of the image. Therefore, it is true that number 5 is the diaphragm for this compound light microscope.Rate this question:
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- 15.
What is the structure letter "n" on the compound light microscope called?
Correct Answer
nosepieceExplanation
The structure letter "n" on the compound light microscope is called the nosepiece. The nosepiece is a rotating component that holds the objective lenses. It allows the user to easily switch between different magnification levels by rotating the nosepiece.Rate this question:
- 16.
What is number 11 on this compound light microscope?
- A.
Arm
- B.
Base
- C.
Fine Adjustment Knob
- D.
Coarse Adjustment Knob
- E.
Low Power Objective Lens
Correct Answer
A. ArmExplanation
The correct answer is "Arm" because the arm is the part of a compound light microscope that connects the eyepiece and the objective lenses. It provides support and stability to the microscope, allowing it to be easily carried or moved.Rate this question:
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- 17.
How many objective lenses are found on this compound light microscope?
- A.
0
- B.
1
- C.
2
- D.
3
- E.
4
Correct Answer
D. 3Explanation
This compound light microscope has three objective lenses. Objective lenses are the lenses closest to the specimen being observed and are responsible for magnifying the image. Having multiple objective lenses with different magnifications allows for greater flexibility and precision in observing different specimens at different levels of detail.Rate this question:
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- 18.
What number is the coarse adjustment knob on this compound light microscope?
- A.
5
- B.
12
- C.
16
- D.
3
- E.
9
Correct Answer
E. 9Explanation
The number 9 is the correct answer because it is the only option that corresponds to the coarse adjustment knob on a compound light microscope. The coarse adjustment knob is used to quickly move the stage up or down to bring the specimen into approximate focus. The other options (5, 12, 16, and 3) do not represent the coarse adjustment knob.Rate this question:
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- 19.
What number is the three objective lens on this compound light microscope?
- A.
5
- B.
12
- C.
16
- D.
3
- E.
9
Correct Answer
D. 3Explanation
The correct answer is 3 because the question is asking for the number of the three objective lens on the compound light microscope. Since the number 3 is listed as one of the options, it is the correct answer.Rate this question:
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- 20.
What is the name of number 7 for this light microscope?
- A.
Revolving nosepiece
- B.
Arm
- C.
Base
- D.
Body tube
Correct Answer
B. ArmExplanation
The correct answer is "Arm" because the arm is the part of a light microscope that connects the base to the body tube and supports the microscope. It provides stability and allows for easy movement and positioning of the microscope.Rate this question:
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- 21.
What is the name of number 9 for this light microscope?
- A.
Revolving nosepiece
- B.
Coarse Adjustment Knob
- C.
Base
- D.
Fine Adjustment Knob
Correct Answer
B. Coarse Adjustment KnobExplanation
The correct answer is "Coarse Adjustment Knob" because it is the part of the light microscope that is typically used to make large, initial adjustments to the focus of the specimen. It allows the user to quickly move the objective lens closer or further away from the specimen to achieve a rough focus. This is in contrast to the "Fine Adjustment Knob," which is used for smaller, more precise adjustments to the focus.Rate this question:
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- 22.
What is the name of number 6 for this light microscope?
- A.
Low-power Objective
- B.
High-power Objective
- C.
Base
- D.
Fine Adjustment Knob
- E.
Light Source
Correct Answer
E. Light SourceExplanation
The correct answer is Light Source. In a light microscope, the light source is an essential component that provides illumination to the specimen being observed. It could be a built-in light bulb or a mirror that reflects external light onto the specimen. The light source plays a crucial role in enabling clear visualization of the specimen and enhancing the contrast for better observation and analysis.Rate this question:
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- 23.
What is the name of number 8 for this light microscope?
- A.
Low-power Objective
- B.
High-power Objective
- C.
Base
- D.
Microscope Stage Clip
- E.
Light Source
Correct Answer
D. Microscope Stage ClipExplanation
The correct answer is Microscope Stage Clip. The microscope stage clip is a small device that holds the slide in place on the stage of a light microscope. It is used to secure the slide and prevent it from moving during observation.Rate this question:
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- 24.
What is the name of number 10 for this light microscope?
- A.
Low-power Objective
- B.
High-power Objective
- C.
Base
- D.
Microscope Stage
- E.
Fine Adjustment Knob
Correct Answer
E. Fine Adjustment KnobExplanation
The fine adjustment knob is used to make small, precise adjustments to the focus of the microscope. It allows for fine-tuning of the image and is typically used after using the coarse adjustment knob to bring the specimen into rough focus. The other options listed are different parts of a light microscope, but they do not refer to the specific function of adjusting the focus.Rate this question:
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- 25.
Stage Clips is the name of letter J on this microscope diagram?
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
The name "Stage Clips" refers to the letter J on the microscope diagram.Rate this question:
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- 26.
Why should a cover-slip and glass microscope slide by held by their edges?
- A.
Sharp edges will cut your fingers
- B.
Reduces the amount of "air bubbles" in the viewing area
- C.
So your fingers will not get stain on them
- D.
Keeps the microscope stage clean
- E.
Allows for a clear image of the specimen
Correct Answer
E. Allows for a clear image of the specimenExplanation
Holding the cover-slip and glass microscope slide by their edges allows for a clear image of the specimen. This is because touching the glass surface with fingers can leave smudges or fingerprints, which can obstruct the view and make the image unclear. By holding the slide and cover-slip by their edges, the glass surfaces remain clean and free from any potential smudges, ensuring a clear and unobstructed view of the specimen under the microscope.Rate this question:
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- 27.
Explain why a specimen to be viewed under the light microscope must be thin?
- A.
Allows light to pass through specimen
- B.
Allow light to pass through the microscope stage
- C.
Increases resolution of the light microscope
- D.
Increases magnification of the light microscope
- E.
Just an old practice from the 1800's
Correct Answer
A. Allows light to pass through specimenExplanation
A specimen to be viewed under the light microscope must be thin because it allows light to pass through the specimen. This is essential for the microscope to capture a clear image of the specimen. If the specimen is too thick, the light will be blocked or scattered, resulting in a blurry or distorted image. By ensuring that the specimen is thin, the light can pass through it easily, allowing for better visualization and analysis under the microscope.Rate this question:
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- 28.
When the TOTAL MAGNIFICATION is calculated during an experiment. Identify the two most correct answers used in the formula.
- A.
Ocular magnification
- B.
Objective magnification
- C.
Length of the low power objective lens
- D.
Length of the high power objective lens
Correct Answer(s)
A. Ocular magnification
B. Objective magnificationExplanation
The total magnification in an experiment is calculated by multiplying the ocular magnification and the objective magnification. The ocular magnification refers to the magnification of the eyepiece, while the objective magnification refers to the magnification of the objective lens. These two factors are multiplied together to determine the overall magnification of the specimen being observed. The length of the low power objective lens and the length of the high power objective lens are not directly involved in the calculation of total magnification.Rate this question:
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- 29.
When viewing a specimen under HIGH POWER. Use the fine adjustment knob only to focus.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
When viewing a specimen under high power, it is recommended to use the fine adjustment knob only to focus. This is because the high power objective lens provides a higher magnification, which results in a narrower field of view and a smaller depth of field. Therefore, using the coarse adjustment knob may cause the specimen to move out of focus quickly. The fine adjustment knob allows for precise and small adjustments to achieve clear focus without disturbing the position of the specimen.Rate this question:
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- 30.
Identify the first step to create a wet mount of the "letter e".
- A.
Add a drop of water to the slide
- B.
Place cover slip over the letter "e" at a 45 degree angle
- C.
Use clean glass slide and cover slip
- D.
Place glass slide on the microscope stage
- E.
Cut out the smallest "letter e" and place on a clean glass slide
Correct Answer
E. Cut out the smallest "letter e" and place on a clean glass slide -
- 31.
Any object observed under a microscope can be described in the word small, but precisely how small? Which unit listed below is used by many scientists?
- A.
Centimeter
- B.
Millimeter
- C.
Micron
- D.
Decimeter
- E.
Myriameter
Correct Answer
C. MicronExplanation
The unit used by many scientists to describe the small size of objects observed under a microscope is the micron. The micron, also known as the micrometer, is equal to one millionth of a meter. It is commonly used in scientific research and microscopy to measure the size of cells, bacteria, and other microscopic structures.Rate this question:
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- 32.
Using the ocular at 10X and scanning objective lens at 4X. You place a transparent plastic ruler on the stage and focus on the mm marks. You can visually count 4 mm across the diameter of your field of view. How many microns is the field of view?
- A.
500
- B.
1 000
- C.
1 500
- D.
4 000
- E.
4 800
Correct Answer
D. 4 000Explanation
When using the ocular at 10X and the scanning objective lens at 4X, you are magnifying the image by a total factor of 40X (10X * 4X = 40X). Since you can visually count 4 mm across the diameter of your field of view, you can calculate the total field of view by multiplying the diameter (4 mm) by the magnification factor (40X). Therefore, the field of view is 160 mm (4 mm * 40X). However, the question asks for the field of view in microns, so you need to convert the result to microns by multiplying by 1000. Therefore, the field of view is 160,000 microns, which is equivalent to 4,000 microns.Rate this question:
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- 33.
Using the ocular at 10X and low power objective lens at 10X. You place a transparent plastic ruler on the stage and focus on the mm marks. You can visually count 1.5 mm across the diameter of your field of view. How many microns is the field of view?
- A.
500
- B.
1 000
- C.
1 500
- D.
4 000
- E.
4 800
Correct Answer
C. 1 500Explanation
When using the ocular at 10X and the low power objective lens at 10X, the total magnification is 100X (10X ocular x 10X objective). The diameter of the field of view is the distance across the circular area that is visible through the microscope. Since you can visually count 1.5 mm across the diameter of the field of view, and the total magnification is 100X, the field of view is 1.5 mm / 100 = 0.015 mm. Since there are 1,000 microns in a millimeter, the field of view is 0.015 mm x 1,000 = 15 microns. Therefore, the correct answer is 1,500 microns.Rate this question:
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- 34.
Using the ocular at 10X and high power objective lens at 40X. You place a transparent plastic ruler on the stage and focus on the mm marks. You can visually count 0.5 mm across the diameter of your field of view. How many microns is the field of view?
- A.
500
- B.
1 000
- C.
1 500
- D.
4 000
- E.
4 800
Correct Answer
A. 500Explanation
When using the ocular at 10X and the high power objective lens at 40X, you can visually count 0.5 mm across the diameter of your field of view. Since 1 mm is equal to 1000 microns, the field of view in microns would be 0.5 mm x 1000 microns/mm = 500 microns.Rate this question:
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- 35.
What do electron microscopes use to 'illuminate' specimens
- A.
Electromagnetic radiation
- B.
Light
- C.
Electrons
- D.
Air
- E.
Neutrons
Correct Answer
C. ElectronsExplanation
Electron microscopes use electrons to 'illuminate' specimens. Unlike light microscopes that use photons, electron microscopes use a beam of accelerated electrons to create a highly magnified image of the specimen. The electrons interact with the specimen, producing signals that are detected and converted into an image. This allows for higher resolution and greater detail compared to traditional light microscopes.Rate this question:
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- 36.
Specimens have to be what in order to make them visible under a light microscope?(NOTE: Please enter your answer in all lower case letters.)
Correct Answer
stained, colored, stainExplanation
To make specimens visible under a light microscope, they need to be stained or colored. Staining involves applying a dye or a chemical to the specimen, which enhances contrast and allows for better visualization of cellular structures. This process helps to highlight specific components or features of the specimen that would otherwise be difficult to see. The staining method is commonly used in microscopy to improve the clarity and visibility of microscopic samples.Rate this question:
- 37.
Which order should the path of light travel starting with the eyepiece?
- A.
Eyepiece lens, objective lens, specimen, illuminator, condenser
- B.
Eyepiece lens, objective lens, condenser, illuminator, specimen
- C.
Eyepiece lens, objective lens, specimen, condenser, illuminator
- D.
Eyepiece lens, illuminator, condenser,objective lens, specimen,
Correct Answer
C. Eyepiece lens, objective lens, specimen, condenser, illuminator -
- 38.
Can electron microscopes be used on living samples?
- A.
Yes
- B.
No
Correct Answer
B. NoExplanation
There is a vacuum inside the microscope because electrons can be scattered by air.Rate this question:
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- 39.
Why must specimens be stained with heavy metals?
- A.
So the electrons stick to the specimen
- B.
Because the electrons are reflected off the specimen
- C.
Because the specimens are transparent to electrons
Correct Answer
C. Because the specimens are transparent to electronsExplanation
Specimens must be stained with heavy metals because they are transparent to electrons. This means that without staining, electrons would pass through the specimens without interacting with them, making them difficult to visualize or study. Staining with heavy metals allows the electrons to be absorbed or scattered by the specimen, making it easier to observe and analyze under an electron microscope.Rate this question:
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- 40.
Light microscopes have a resolution good enough to see what?
- A.
Cells
- B.
Tissues
- C.
Protein chains
- D.
DNA
Correct Answer(s)
A. Cells
B. TissuesExplanation
Light microscopes have a resolution good enough to see cells and tissues. Light microscopes use visible light to magnify and illuminate specimens, allowing scientists to observe and study the structure and behavior of cells and tissues. The resolution of a microscope determines the level of detail that can be seen, and although light microscopes have limitations compared to electron microscopes, they are still capable of providing valuable information about the organization and functioning of cells and tissues.Rate this question:
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