Lab 8- Structural Staining
Strucutural staining, for Microbiology 221
Lab # 8
Questions and Answers
- 1.
Which is Bacillus?
- A.
Tetnus
- B.
Anthrax
- C.
Botulism
- D.
Diarrhea
Correct Answer
B. AnthraxExplanation
Bacillus anthracisRate this question:
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- 2.
What stain is used in an Endospore stain?
Correct Answer
Schaffer-Fulton MethodExplanation
The Schaffer-Fulton Method is used in an Endospore stain. This staining method involves the use of malachite green as the primary stain, which specifically stains endospores. The stain is applied to the heat-fixed bacterial smear and then heated to facilitate the penetration of the stain into the endospores. After rinsing, the smear is counterstained with safranin, which stains the vegetative cells. This differential staining technique allows for the visualization and differentiation of endospores from the rest of the bacterial cells.Rate this question:
- 3.
Which Stain colors the Flagella structures?
- A.
Fleulgen stain
- B.
Endospore stain
- C.
Acid Fast stain
- D.
Flagellar Stain
Correct Answer
D. Flagellar StainExplanation
Flagellar Stain is the correct answer because it is specifically designed to color the flagella structures of bacteria. This staining technique helps in visualizing and studying the flagella, which are whip-like structures responsible for bacterial motility. By using a flagellar stain, scientists can observe the arrangement, number, and distribution of flagella on bacterial cells, aiding in the identification and classification of different bacterial species based on their flagellar characteristics.Rate this question:
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- 4.
Feulgen Stain stains what?
Correct Answer
DNA structuresExplanation
The Feulgen Stain is a specific stain used in cytology to stain DNA structures. It binds to the DNA present in the cells, allowing for visualization and identification of DNA structures under a microscope. This stain is commonly used in research and diagnostic laboratories to study and analyze DNA content in various biological samples.Rate this question:
- 5.
How long does the Endospore Cycle take?
- A.
14 hrs
- B.
16hrs
- C.
10hrs
- D.
2hrs
- E.
8hrs
Correct Answer
B. 16hrsExplanation
The correct answer is 16hrs. The endospore cycle refers to the process of endospore formation, which involves the transformation of a bacterial cell into a dormant and highly resistant structure called an endospore. This process typically takes around 16 hours to complete. During this time, the bacterial cell undergoes various stages, including DNA replication, septum formation, engulfment, cortex synthesis, and coat formation, leading to the formation of a mature endospore. Therefore, the correct answer is 16hrs.Rate this question:
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- 6.
What is Germination or Cryptobiosis, and how long does it take?
- A.
Spreading of disease, 2 hrs
- B.
Moving bacteria from one plate to another, 1 hr
- C.
Becoming active cell structure, 8hrs
- D.
Cell dying in autoclave, 5hrs
Correct Answer
C. Becoming active cell structure, 8hrsExplanation
Germination or cryptobiosis refers to the process of a cell or organism becoming active and functioning. In this context, it takes approximately 8 hours for the cell to become an active cell structure. This suggests that after a period of dormancy or inactivity, the cell undergoes a transformation and starts performing its intended functions.Rate this question:
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- 7.
Clostridium botulinum- Botulism is what?
- A.
Anerobic
- B.
Gas Gangrene
- C.
Aerobic
- D.
A Virus
Correct Answer
A. AnerobicExplanation
Clostridium botulinum is a bacterium that causes botulism, a rare but serious illness. The bacterium is anaerobic, meaning it can survive and grow in environments with little to no oxygen. This characteristic allows it to thrive in certain conditions such as improperly canned or preserved foods. Gas gangrene, on the other hand, is caused by a different bacterium called Clostridium perfringens, which is also anaerobic. Aerobic refers to organisms that require oxygen to survive, and botulism is not caused by a virus. Therefore, the correct answer is anaerobic.Rate this question:
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- 8.
Clostridium Difficle is...*unswollen, terminal
Correct Answer
DiarrheaExplanation
Clostridium difficile is a bacterium that causes diarrhea. It is not related to being unswollen or terminal. This bacterium is commonly found in hospitals and healthcare settings and can cause severe inflammation of the colon, leading to diarrhea. It is often associated with the use of antibiotics, as they disrupt the natural balance of bacteria in the gut, allowing Clostridium difficile to overgrow and cause infection. The symptoms of Clostridium difficile infection include watery diarrhea, fever, loss of appetite, abdominal pain, and sometimes blood or mucus in the stool.Rate this question:
- 9.
What is Clostridium tetani?*swollen, terminal
Correct Answer
tetanus - 10.
What is gas Gangrene?
- A.
Aerobic
- B.
Protazoa
- C.
Bacillus anthracis
- D.
Colstridium pergringens
Correct Answer
D. Colstridium pergringensExplanation
Gas gangrene is a severe and potentially life-threatening infection caused by Clostridium perfringens bacteria. This bacterium is anaerobic, meaning it can survive and multiply in environments with little to no oxygen. Gas gangrene occurs when the bacteria enter deep tissues through a wound, usually one that is contaminated with dirt or debris. Once inside the body, the bacteria release toxins that destroy tissue and cause the production of gas bubbles. This leads to symptoms such as severe pain, swelling, dark-colored skin, and a foul-smelling discharge. Without prompt treatment, gas gangrene can rapidly spread and cause tissue death, leading to serious complications or even death.Rate this question:
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- 11.
DPA is..
Correct Answer
Dipicolinic AcidExplanation
Dipicolinic Acid (DPA) is a chemical compound commonly found in bacterial spores. It plays a crucial role in the heat resistance of spores and is responsible for their ability to withstand extreme conditions. DPA acts as a calcium chelator, binding to calcium ions and forming a complex that stabilizes the spore's DNA. This complex helps protect the genetic material from damage during spore formation and germination. DPA is often used as a biomarker for the presence of bacterial spores and is extensively studied for its potential applications in various fields, including food safety and biodefense.Rate this question:
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