Immunological Diagnostic 2: PCR And Quick Tests

19 Questions

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Immunology Quizzes & Trivia

Questions and Answers
  • 1. 
    The Polymerase Chain Reaction results is exponential multiplication of the DNA chain
    • A. 

      True

    • B. 

      False

  • 2. 
    The Polymerase Chain Reaction is reliant on the action of primers (nucleotide sequences which act to amplify the translation of a certain gene)
    • A. 

      True

    • B. 

      False

  • 3. 
    Taq polymerase is stable at 142oC
    • A. 

      True

    • B. 

      False

  • 4. 
    DNA primers are non-specific to genes
    • A. 

      True

    • B. 

      False

  • 5. 
    Taq polymerases activity is dependant on temperature
    • A. 

      True

    • B. 

      False

  • 6. 
    DNA primers are 25 - 35 nucleotides long
    • A. 

      True

    • B. 

      False

  • 7. 
    DNA primers bind to the sense strand of DNA
    • A. 

      True

    • B. 

      False

  • 8. 
    Which is the first step in the polymerase chain reaction?
    • A. 

      DNA is heated to around 80oC, causing the strands to come apart

    • B. 

      Viral gene translation is amplified

    • C. 

      As the mixture cools, Taq polymerase starts to work

    • D. 

      Sites for Taq polymerase on DNA are exposed

    • E. 

      Heating and cooling process is repeated 30 - 40 times

  • 9. 
    Which is the second step in the polymerase chain reaction?
    • A. 

      DNA is heated to around 80oC, causing the strands to come apart

    • B. 

      Viral gene translation is amplified

    • C. 

      As the mixture cools, Taq polymerase starts to work

    • D. 

      Sites for Taq polymerase on DNA are exposed

    • E. 

      Heating and cooling process is repeated 30 - 40 times

  • 10. 
    Which is the third step in the polymerase chain reaction?
    • A. 

      DNA is heated to around 80oC, causing the strands to come apart

    • B. 

      Viral gene translation is amplified

    • C. 

      As the mixture cools, Taq polymerase starts to work

    • D. 

      Sites for Taq polymerase on DNA are exposed

    • E. 

      Heating and cooling process is repeated 30 - 40 times

  • 11. 
    Which is the fourth step in the polymerase chain reaction?
    • A. 

      DNA is heated to around 80oC, causing the strands to come apart

    • B. 

      Viral gene translation is amplified

    • C. 

      As the mixture cools, Taq polymerase starts to work

    • D. 

      Sites for Taq polymerase on DNA are exposed

    • E. 

      Heating and cooling process is repeated 30 - 40 times

  • 12. 
    Which is the fifth step in the polymerase chain reaction?
    • A. 

      DNA is heated to around 80oC, causing the strands to come apart

    • B. 

      Viral gene translation is amplified

    • C. 

      As the mixture cools, Taq polymerase starts to work

    • D. 

      Sites for Taq polymerase on DNA are exposed

    • E. 

      Heating and cooling process is repeated 30 - 40 times

  • 13. 
    What gel is typically used during electrophoresis?
    • A. 

      Agar gel

    • B. 

      Hydro gel

    • C. 

      Sucarose gel

    • D. 

      Agarose gel

  • 14. 
    What is the principles by which electrophoresis of DNA works?
    • A. 

      DNA is negatively charged, hence it moves toward the positively charged area; larger DNA migrates at a rate faster than smaller DNA

    • B. 

      DNA is positively charged, hence it moves toward the negatively charged area; larger DNA migrates at a rate slower than smaller DNA

    • C. 

      DNA is negatively charged, hence it moves toward the positively charged area; larger DNA migrates at a rate slower than smaller DNA

    • D. 

      DNA is positively charged, hence it moves toward the negatively charged area; larger DNA migrates at a rate faster than smaller DNA

  • 15. 
    Which of the following is not an advantage of PCT?
    • A. 

      Does not give false positive results

    • B. 

      Highly sensitive

    • C. 

      Can be performed soon after infection

    • D. 

      Does not rely on antibody production

  • 16. 
    Which of the following is not a disadvantage of PCT?
    • A. 

      Can be too sensitive; a small amount of contamination can give a +ve signal

    • B. 

      Expensive to perform

    • C. 

      Quick to perform; can easily be automated

    • D. 

      Need to extract DNA

  • 17. 
    How long does it take for a PCT to be possible post-infection?
    • A. 

      1 week

    • B. 

      2 weeks

    • C. 

      3 weeks

    • D. 

      4 weeks

    • E. 

      5 weeks

    • F. 

      6 weeks

  • 18. 
    What is the purpose of reverse transciptases in RT-PCT?
    • A. 

      Allows RNA signal to be amplified

    • B. 

      Converts viral DNA into RNA

    • C. 

      Converts RNA to DNA as it is unstable (to heating process???)

    • D. 

      Something

  • 19. 
    HIV is not a retrovirus or RNA virus, therefore PCT is used as opposed to RT-PCT when testing for it.
    • A. 

      True

    • B. 

      False