Bio: Lab 2 The Microscope

The 4x Objective is commonly known as the Scanning Objective in microscopy as it is typically used for low magnification to scan the specimen quickly.

In microscopy, the 10x objective lens is often referred to as the Low Objective because it has a lower magnification compared to higher objectives like the 40x or 100x.

In microscopy, the term 'High Objective' refers to the magnification power of a 40x objective lens.

The total magnification is calculated by multiplying the magnification power of the ocular lens and the objective lens being used.

Wet mounts or cover slides are created using self-prepared slides, which involve preparing and placing samples on glass slides before covering them with a cover slip for observation under a microscope.

Permanent slides are also known as prepared slides that are created to be long-lasting for viewing under a microscope.

As magnification increases, the image becomes larger and more detailed, leading to a decrease in the amount of light reaching the viewer's eye.

The Field of View at 10x magnification refers to the diameter of the visible circle when looking through a microscope at that level of magnification. In this case, the correct answer is that the diameter is 1.8mm, providing a specific measurement for the field of view at 10x magnification.

Field of View (FOV) is calculated by the formula: FOV (1.8mm) x Mag (@10x) = FOV (?) x Mag (@?). The correct formula to calculate FOV at a specific magnification is FOV@x = 18/x. For example, FOV@4x = 4.5mm and FOV@40x = 0.45mm.

Cytology specifically focuses on the structure and function of cells, utilizing various microscopy techniques and cell chemistry analysis.

Depth of field refers to the range of distance that appears acceptably sharp in an image. In photography, low power settings provide a greater depth of field, allowing more of the image to be in focus. High power settings result in a shallow depth of field, where only a small portion of the image is in focus. Therefore, low power provides the greatest depth of field.

The magnification of an image viewed through the 40x Objective is calculated by multiplying the magnification of the objective lens (40x) with the magnification of the eyepiece (10x). Therefore, the correct answer is 400 times (40 x 10).

Resolution refers to the ability to distinguish 2 points as separate points, which is important for clarity and detail in images.

Contrast refers to the difference between the lightest and darkest parts of an image, highlighting the range of tones present.

The condenser lens in a microscope is responsible for focusing light just below the specimen to ensure proper illumination and clarity of the specimen being viewed.

Depth of Field in photography refers to the range of distance that appears acceptably sharp in an image. It is determined by the aperture setting, focal length, and distance between the camera and the subject.