Bsl3 Quiz - Refresher-facilities
Policies & Procedures for Biosafety Level 3 Laboratory
- 1.Scientists with BSL-3 access are working with airborne pathogens.
- A.
True
- B.
False
- 2.In which of the following items will you definitely find non-inactived pathogens?
- A.
Incubators
- B.
Fridges/Freezers
- C.
Biosafety Cabinet
- D.
Waste Containers
- 3.Where else could you potentially encounter pathogens in the BSL-3?
- A.
Centrifuges
- B.
In the anteroom
- C.
On the door handles
- D.
Under the fridges
- 4.Where can you find the needlestick hotline number?
- A.
On each phone of the BSL3
- B.
Posted in the ante room and BSL3 room
- C.
On the first page of the BSL3 manual
- 5.It is OK to give access to the BSL-3 to anyone who asks.
- A.
True
- B.
False
- 6.Minors can access the BSL-3 if they are accompanied by an adult.
- A.
True
- B.
False
- 7.The Proper Personnel Protective Equipment for the BSL-3 includes:
- A.
A gown and one pair of gloves
- B.
A gown, a mask, and one pair of gloves
- C.
A gown, two pairs of gloves, and safety glasses
- D.
A gown, two pairs of gloves, a respirator, and safety glasses
- 8.It is OK to use a cell phone or an iPod in the BSL-3 if a user removes their outer gloves when holding it.
- A.
True
- B.
False
- 9.To better notice potential defects or tears in outer gloves, the inside gloves should be of a darker color.
- A.
True
- B.
False
- 10.What are the two liquids used as decontaminants in the BSL-3?
- A.
Soapy water
- B.
Ethanol 70
- C.
Bleach
- D.
Antibiotic soap
- 11.What do you do if you find tubes when you move a piece of equipment?
- A.
If it's closed and not leaking, take a broom from the custodian room and push it out of the way.
- B.
If it's closed and not leaking, leave it be.
- C.
If it's leaking, place a pad over it, pour 10% bleach over it, wait 30 minutes, and discard.
- D.
If it's leaking, pour 10% bleach over it and discard.
- 12.Order the step of the to clean a biohazard spill a. Add 10% bleach to soak the spill control pad and let it react for 30 minutes. b. Place the spill control pad over liquid to absorb and control the spread of liquid. c. Carefully remove pad, place into doubled autoclave bags. d. Dispose of clean-up materials appropriately. e. Decontaminate all exposed surfaces with 10% bleach disinfectant and rinse with 70% ethanol. f. Remove personnel protective equipment (PPE), wash hands, and exit BSL-3 laboratory to the anteroom.
- A.
A,b,c,d,e,f
- B.
B,a,c,d,e,f
- 13.What pathogens are handled in the Gladstone BSL-3?
- A.
HIV
- B.
HCV
- C.
Ebola
- D.
OPIM (Other Potentially Infectious Materials)
- E.
Herpes B
- 14.Who is responsible for the safety of maintenance personnel that you have given access to the BSL-3?
- A.
Mauricio Montano, the BLS-3 Manager.
- B.
Reuben Oregon, Facilities Engineer.
- C.
You, i.e. the person who gives access.
- D.
The most senior-level scientist currently working in the BLS-3.
- 15.What do you need to do before you enter the BSL3 with a repair technician to perform a maintenance task?
- A.
Read and sign Appendix 4 of the BSL3 manual also located in anteroom.
- B.
Find Mauricio Montano to get permission.
- C.
Make sure no one is working in the BSL-3.
- 16.What do you do if you see someone that you don’t know in the BSL-3?
- A.
Ask them who they are and what they are doing.
- B.
Call Mauricio Montano or someone from Facilities.
- C.
Don't bother them as they clearly got in, so must have access privileges.
- D.
Introduce yourself.
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