Bsl3 Quiz - Refresher-facilities
Policies & Procedures for Biosafety Level 3 Laboratory
Questions and Answers
- 1.
Scientists with BSL-3 access are working with airborne pathogens.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
Scientists with BSL-3 access are not necessarily working with airborne pathogens. BSL-3 (Biosafety Level 3) is a containment level designed for work with microorganisms that can cause serious or potentially lethal diseases through inhalation. This includes pathogens that are transmitted through the air, but it also includes pathogens that can be transmitted through other routes such as direct contact or ingestion. Therefore, scientists with BSL-3 access may be working with a variety of pathogens, not exclusively airborne ones.Rate this question:
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- 2.
In which of the following items will you definitely find non-inactived pathogens?
- A.
Incubators
- B.
Fridges/Freezers
- C.
Biosafety Cabinet
- D.
Waste Containers
Correct Answer(s)
A. Incubators
B. Fridges/Freezers
C. Biosafety Cabinet
D. Waste ContainersExplanation
In all of the given items, there is a possibility of finding non-inactivated pathogens. Incubators provide a warm and humid environment, which can support the growth of pathogens. Fridges/Freezers may not always be able to completely eliminate pathogens, especially if they are not properly cleaned and maintained. Biosafety cabinets are used for handling hazardous materials, including pathogens, so there is a chance of finding non-inactivated pathogens in them. Waste containers can also contain non-inactivated pathogens if proper disposal protocols are not followed.Rate this question:
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- 3.
Where else could you potentially encounter pathogens in the BSL-3?
- A.
Centrifuges
- B.
In the anteroom
- C.
On the door handles
- D.
Under the fridges
Correct Answer(s)
A. Centrifuges
D. Under the fridgesExplanation
In a BSL-3 facility, which is designed to handle moderately hazardous pathogens, there are various areas where one could potentially encounter pathogens. Centrifuges are commonly used in laboratories to separate substances, and if they are contaminated with pathogens, they can pose a risk. Additionally, under the fridges is another potential area where pathogens can accumulate if not properly cleaned. However, the anteroom and door handles are not mentioned as potential areas for encountering pathogens in the BSL-3 facility.Rate this question:
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- 4.
Where can you find the needlestick hotline number?
- A.
On each phone of the BSL3
- B.
Posted in the ante room and BSL3 room
- C.
On the first page of the BSL3 manual
Correct Answer(s)
A. On each phone of the BSL3
B. Posted in the ante room and BSL3 room
C. On the first page of the BSL3 manualExplanation
The needlestick hotline number can be found in three different places: on each phone of the BSL3, posted in the ante room and BSL3 room, and on the first page of the BSL3 manual. These locations ensure that the number is easily accessible and visible to anyone who may need it in case of a needlestick incident.Rate this question:
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- 5.
It is OK to give access to the BSL-3 to anyone who asks.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
Giving access to the BSL-3 (Biosafety Level 3) to anyone who asks is not okay. BSL-3 is a high-containment laboratory designed for handling and studying dangerous pathogens. It requires specialized training, knowledge, and adherence to strict safety protocols. Granting access to individuals without proper training and authorization can pose significant risks to both the person and the surrounding environment. Therefore, it is not acceptable to allow anyone to have access to the BSL-3 facility.Rate this question:
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- 6.
Minors can access the BSL-3 if they are accompanied by an adult.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
Minors cannot access the BSL-3 even if they are accompanied by an adult.Rate this question:
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- 7.
The Proper Personnel Protective Equipment for the BSL-3 includes:
- A.
A gown and one pair of gloves
- B.
A gown, a mask, and one pair of gloves
- C.
A gown, two pairs of gloves, and safety glasses
- D.
A gown, two pairs of gloves, a respirator, and safety glasses
Correct Answer
C. A gown, two pairs of gloves, and safety glassesExplanation
The correct answer is a gown, two pairs of gloves, and safety glasses. In a BSL-3 (Biosafety Level 3) environment, which involves working with potentially infectious materials, it is important to have proper personnel protective equipment. A gown helps to protect the body from contamination, while two pairs of gloves provide an extra layer of protection for the hands. Safety glasses are necessary to protect the eyes from any potential splashes or aerosols. This combination of protective equipment ensures that the personnel are adequately protected while working in a BSL-3 setting.Rate this question:
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- 8.
It is OK to use a cell phone or an iPod in the BSL-3 if a user removes their outer gloves when holding it.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
Using a cell phone or an iPod in a BSL-3 facility is not okay even if the user removes their outer gloves. BSL-3 facilities are designed to handle moderately hazardous pathogens, and the use of personal electronic devices can introduce contaminants and compromise the safety of the environment. It is important to follow the protocols and guidelines in place to ensure the integrity of the facility and the safety of all personnel working in it.Rate this question:
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- 9.
To better notice potential defects or tears in outer gloves, the inside gloves should be of a darker color.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
The statement suggests that using darker colored inside gloves can help in better noticing potential defects or tears in outer gloves. Darker colors create a contrast with lighter colors, making it easier to identify any imperfections. This can be particularly useful in industries where gloves are commonly used, such as healthcare or manufacturing, as it allows for quick identification of any damage that may compromise safety or hygiene.Rate this question:
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- 10.
What are the two liquids used as decontaminants in the BSL-3?
- A.
Soapy water
- B.
Ethanol 70
- C.
Bleach
- D.
Antibiotic soap
Correct Answer(s)
B. Ethanol 70
C. BleachExplanation
Ethanol 70 and bleach are the two liquids used as decontaminants in the BSL-3. Ethanol 70 is a common disinfectant that is effective against a wide range of microorganisms. It is commonly used to disinfect surfaces and equipment in laboratories. Bleach, on the other hand, is a strong oxidizing agent that can kill a variety of pathogens, including bacteria, viruses, and fungi. It is often used to decontaminate surfaces and materials in high-risk areas such as BSL-3 laboratories.Rate this question:
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- 11.
What do you do if you find tubes when you move a piece of equipment?
- A.
If it's closed and not leaking, take a broom from the custodian room and push it out of the way.
- B.
If it's closed and not leaking, leave it be.
- C.
If it's leaking, place a pad over it, pour 10% bleach over it, wait 30 minutes, and discard.
- D.
If it's leaking, pour 10% bleach over it and discard.
Correct Answer(s)
A. If it's closed and not leaking, take a broom from the custodian room and push it out of the way.
C. If it's leaking, place a pad over it, pour 10% bleach over it, wait 30 minutes, and discard.Explanation
The correct answer suggests that if the tubes are closed and not leaking, the appropriate action is to take a broom from the custodian room and push them out of the way. This ensures that the tubes are not obstructing the equipment and allows for smooth movement. On the other hand, if the tubes are leaking, the correct course of action is to place a pad over them, pour 10% bleach over it, wait 30 minutes, and then discard the tubes. This helps to contain any potential hazardous material and ensures proper disposal.Rate this question:
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- 12.
Order the step of the to clean a biohazard spill a. Add 10% bleach to soak the spill control pad and let it react for 30 minutes. b. Place the spill control pad over liquid to absorb and control the spread of liquid. c. Carefully remove pad, place into doubled autoclave bags. d. Dispose of clean-up materials appropriately. e. Decontaminate all exposed surfaces with 10% bleach disinfectant and rinse with 70% ethanol. f. Remove personnel protective equipment (PPE), wash hands, and exit BSL-3 laboratory to the anteroom.
- A.
A,b,c,d,e,f
- B.
B,a,c,d,e,f
Correct Answer
B. B,a,c,d,e,fExplanation
First, the spill control pad should be placed over the liquid to absorb and control the spread of the biohazard spill (step b). Then, 10% bleach should be added to soak the spill control pad and let it react for 30 minutes (step a). Carefully remove the pad and place it into doubled autoclave bags (step c). Dispose of the clean-up materials appropriately (step d). Decontaminate all exposed surfaces with 10% bleach disinfectant and rinse with 70% ethanol (step e). Finally, remove personal protective equipment (PPE), wash hands, and exit the BSL-3 laboratory to the anteroom (step f).Rate this question:
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- 13.
What pathogens are handled in the Gladstone BSL-3?
- A.
HIV
- B.
HCV
- C.
Ebola
- D.
OPIM (Other Potentially Infectious Materials)
- E.
Herpes B
Correct Answer(s)
A. HIV
B. HCV
D. OPIM (Other Potentially Infectious Materials)
E. Herpes BExplanation
The Gladstone BSL-3 handles HIV, HCV, OPIM (Other Potentially Infectious Materials), and Herpes B pathogens. BSL-3 stands for Biosafety Level 3, which is a containment level used for handling pathogens that can cause serious diseases in humans. HIV and HCV are viruses that can lead to chronic infections and potentially life-threatening conditions. OPIM refers to any materials that may contain infectious agents, such as tissues or bodily fluids. Herpes B is a virus primarily found in non-human primates but can be transmitted to humans, causing severe neurological diseases. Therefore, the Gladstone BSL-3 is equipped to handle these pathogens safely and prevent their spread.Rate this question:
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- 14.
Who is responsible for the safety of maintenance personnel that you have given access to the BSL-3?
- A.
Mauricio Montano, the BLS-3 Manager.
- B.
Reuben Oregon, Facilities Engineer.
- C.
You, i.e. the person who gives access.
- D.
The most senior-level scientist currently working in the BLS-3.
Correct Answer
C. You, i.e. the person who gives access.Explanation
The correct answer is "You, i.e. the person who gives access." The responsibility for the safety of maintenance personnel lies with the person who grants them access to the BSL-3 facility. This person is in a position of authority and is responsible for ensuring that proper safety protocols are followed and that the maintenance personnel are aware of the potential hazards and equipped with the necessary safety measures.Rate this question:
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- 15.
What do you need to do before you enter the BSL3 with a repair technician to perform a maintenance task?
- A.
Read and sign Appendix 4 of the BSL3 manual also located in anteroom.
- B.
Find Mauricio Montano to get permission.
- C.
Make sure no one is working in the BSL-3.
Correct Answer
A. Read and sign Appendix 4 of the BSL3 manual also located in anteroom.Explanation
Before entering the BSL3 with a repair technician to perform a maintenance task, it is necessary to read and sign Appendix 4 of the BSL3 manual, which is also located in the anteroom. This is important because Appendix 4 likely contains important information and guidelines regarding safety protocols and procedures specific to the BSL3 environment. By reading and signing it, the repair technician ensures that they are aware of and will comply with these protocols, thereby minimizing the risk of accidents or contamination during the maintenance task.Rate this question:
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- 16.
What do you do if you see someone that you don’t know in the BSL-3?
- A.
Ask them who they are and what they are doing.
- B.
Call Mauricio Montano or someone from Facilities.
- C.
Don't bother them as they clearly got in, so must have access privileges.
- D.
Introduce yourself.
Correct Answer(s)
A. Ask them who they are and what they are doing.
B. Call Mauricio Montano or someone from Facilities.Explanation
If you see someone that you don't know in the BSL-3, it is important to ask them who they are and what they are doing to ensure that they have a legitimate reason to be there. Additionally, it is recommended to call Mauricio Montano or someone from Facilities to report the situation and seek assistance in handling the situation appropriately. This helps to maintain the security and safety protocols in the BSL-3 facility.Rate this question:
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