Quiz 6 – Analysis And Uses Of Cloned DNA

20 Questions | Total Attempts: 138

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Quiz 6  Analysis And Uses Of Cloned DNA

Section J – Analysis and uses of cloned DNA SOUTHERN BLOTS &  NORTHERN BLOTS DNA SEQUENCINGQuiz offline susulan dan remidi yang rencananya diselenggarakan besok Senin, 7 Juni 2010, Jam 19.00 WIB DIBATALKAN. Quiz offline susulan dan remidi akan dilaksanakan mulai tanggal 16 sd 21 Juni 2010, jam akan ditentukan kemudian sesuai perjanjian. Bagi Anda yang mengikuti remidi, maka NILAI QUIZ TERAKHIR Anda yang akan dipakai, bukan nilai terbaik.


Questions and Answers
  • 1. 
    Method for transferring DNA fragments onto a membrane for detection of specific sequences by hybridisation.
    • A. 

      Nothern blotting.

    • B. 

      Southern blotting

    • C. 

      Western blotting.

    • D. 

      Eastern blotting

    • E. 

      Zoo blotting

  • 2. 
    The nucleic acid molecules are separated by  agarose gel electrophoresis and then transferred to a nylon or  nitrocellulose membrane.
    • A. 

      TRUE

    • B. 

      FALSE

  • 3. 
    Northern blots can help to identify whether a genomic clone has regions that are transcribed.
    • A. 

      TRUE

    • B. 

      FALSE

  • 4. 
    A procedure  using hybridisation analysis to test the ability of a nucleic acid probe from one species to hybridise with the DNA fragment of another species.
    • A. 

      TRUE

    • B. 

      FALSE

  • 5. 
    A procedure using Southern blot analysis to test the ability of a nucleic acid probe from one species to hybridize with the DNA fragment of another species.
    • A. 

      Nothern blotting

    • B. 

      Southern blotting

    • C. 

      Western blotting

    • D. 

      Eastern blotting

    • E. 

      Zoo blotting

  • 6. 
    The Sanger’s method uses deoxynucleotides as chain terminators to produce a ladder of molecules generated by polymerase extension of a primer.
    • A. 

      TRUE

    • B. 

      FALSE

  • 7. 
    Gilbert chemical method uses end-labeled DNA is subjected to base-specific cleavage reactions by restriction enzyme prior to gel separation.
    • A. 

      TRUE

    • B. 

      FALSE

  • 8. 
    Agarose gel  electrophoresis analysis allows the  sequence to be read.
    • A. 

      TRUE

    • B. 

      FALSE

  • 9. 
    Formic acid will attack the pyrimidines (C + T).
    • A. 

      TRUE

    • B. 

      FALSE

  • 10. 
    Formic acid will attack the pyrimidines (C + T).
    • A. 

      TRUE

    • B. 

      FALSE

  • 11. 
    The Southern blot is used to verify the presence or absence of a specific amino acid sequence in the protein from different sources.
    • A. 

      TRUE

    • B. 

      FALSE

  • 12. 
    The Southern blot is used to identify the size of the restriction fragment that contains the sequence.
    • A. 

      TRUE

    • B. 

      FALSE

  • 13. 
    Probe is a labelled molecule used in hybridisation procedures.
    • A. 

      TRUE

    • B. 

      FALSE

  • 14. 
    The Walter Gilbert method, or dideoxy sequencing, is the most commonly used method of DNA sequencing.
    • A. 

      TRUE

    • B. 

      FALSE

  • 15. 
    Dideoxy sequencing involves the extension of a short DNA primer by RNA polymerase in 4 separate reactions.
    • A. 

      TRUE

    • B. 

      FALSE

  • 16. 
    A dideoxynucleotide differs from a deoxynucleotide in that it has a 3’- OH rather than a 3’- H on the deoxyribose sugar.
    • A. 

      TRUE

    • B. 

      FALSE

  • 17. 
    The absence of a 3’-OH on the dideoxynucleotide prevent the formation of a phosphodiester bond with an incoming DNA nucleotide, so DNA synthesis terminates after the addition of this dideoxynucleotid.
    • A. 

      TRUE

    • B. 

      FALSE

  • 18. 
    When the deoxynucleotide is inserted, elongation of the newly synthesized strand stops.
    • A. 

      TRUE

    • B. 

      FALSE

  • 19. 
    In the Southern blot, the RNA is isolated from each source and then digested with a specific restriction enzyme.
    • A. 

      TRUE

    • B. 

      FALSE

  • 20. 
    In the Southern blot, the RNA is transferred from the fragile gel to a nylon filter.
    • A. 

      TRUE

    • B. 

      FALSE

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