Microbiology Competency_weeks_2012
This quiz is designed to test the basic competencies in Microbiology for a small hospital lab.
- 1.The following organisms are considered possible Bioterrorism organism. Check all that apply.
- A.
Bacillus anthracis
- B.
Staph aureus
- C.
Yersinia pestis
- D.
Strep pneumo
- E.
Francisella tularensis
- F.
E. coli
- G.
Brucella species
- 2.Universal precautions are only necessary when handling specimens that have contamination on the outside of the container.
- A.
True
- B.
False
- 3.Check any of the following that are appropriate reasons for rejecting a specimen for microbiology.
- A.
Unlabeled specimen
- B.
Leaky container
- C.
Wound culture submitted in a culturette containine Modified Ames Stuart media
- D.
Urine specimen delayed transport of 4 hours
- E.
Properly collected clean catch urine delivered immediately to the lab
- F.
A dry swab
- 4.Intracellular gram-negative dipplococci seen on a gram stain of CSF would be suspicious of what organism?
- A.
E. coli
- B.
Neisseria meningococcus
- C.
Haemophilus influenza
- D.
Staph aureus
- 5.Grape-like clusters of gram positive cocci seen on a gram stain would be suspect of which organism?
- A.
Staph species
- B.
Strep species
- C.
Brucella species
- D.
Enterobacteriaceae
- 6.A colorless/clear colony growing on XLD agar should be worked up for which of the following enteric pathogen?
- A.
Salmonella
- B.
Campylobacter
- C.
Shigella
- D.
E. coli
- 7.A black colony found growing on XLD agar should be worked up for which of the following enteric pathogens?
- A.
Salmonella
- B.
Shigella
- C.
Campylobacter
- D.
E. coli
- 8.A clean catch urine sample is growing >100,000 of a single type gram negative rod. This culture should be worked up as positive and the organism should be identified and sensitivities performed.
- A.
True
- B.
False
- 9.A sputum specimen is received and the gram stain is showing >25 squamous epithelials per low power field. What is the appropriate next step?
- A.
Set the culture up
- B.
Notify the appropriate clinician and request a new specimen
- C.
Throw the sample away and don't tell anyone
- D.
Go yell at the nurse for sending you spit
- 10.The most common use of the Catalase test is to:
- A.
Presumptively identify yeast
- B.
Distinguish between Staph and Strep species
- C.
To test for Protein A
- D.
To test for X and V factors
- 11.The Oxidase test is useful in initial characterization of gram negative bacteria. Which of the following would be Oxidase positive?
- A.
E. coli
- B.
Pseudomonas
- C.
Campylobacter
- D.
Klebsiella
- 12.Which statements are true of Beta lactamases? Check all that apply
- A.
Beta lactamases are enzymes produced by certain bacteria that are able to inactivate Beta lactam antibiotics (phnicillins and cephalosporins)
- B.
Testing for Beta lactamase is not a very useful test
- C.
Direct Beta lactamase testing can be used as a rapid and effective method of detecting resistance to beta lactam antibiotics in strains of Staph.aureus, Haemophilus influenza, Neisseria gonorrhea and Moraxella catarrhalis
- D.
Beta lactamase test detects indole production
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