Microbiology Competency_weeks_2012

1. A clean catch urine sample is growing >100,000 of a single type gram negative rod. This culture should be worked up as positive and the organism should be identified and sensitivities performed.

True

False

2. Intracellular gram-negative dipplococci seen on a gram stain of CSF would be suspicious of what organism?

E. coli

Neisseria meningococcus

Haemophilus influenza

Staph aureus

3. Grape-like clusters of gram positive cocci seen on a gram stain would be suspect of which organism?

Staph species

Strep species

Brucella species

Enterobacteriaceae

4. The most common use of the Catalase test is to:

Presumptively identify yeast

Distinguish between Staph and Strep species

To test for Protein A

To test for X and V factors

5. Universal precautions are only necessary when handling specimens that have contamination on the outside of the container.

True

False

6. A sputum specimen is received and the gram stain is showing >25 squamous epithelials per low power field. What is the appropriate next step?

Set the culture up

Notify the appropriate clinician and request a new specimen

Throw the sample away and don't tell anyone

Go yell at the nurse for sending you spit

7. A black colony found growing on XLD agar should be worked up for which of the following enteric pathogens?

Salmonella

Shigella

Campylobacter

E. coli

8. Which statements are true of Beta lactamases? Check all that apply

Beta lactamases are enzymes produced by certain bacteria that are able to inactivate Beta lactam antibiotics (phnicillins and cephalosporins)

Testing for Beta lactamase is not a very useful test

Beta lactamase test detects indole production

Submit

9. Check any of the following that are appropriate reasons for rejecting a specimen for microbiology.

Unlabeled specimen

Leaky container

Wound culture submitted in a culturette containine Modified Ames Stuart media

Urine specimen delayed transport of 4 hours

Properly collected clean catch urine delivered immediately to the lab

A dry swab

Submit

10. A colorless/clear colony growing on XLD agar should be worked up for which of the following enteric pathogen?

Salmonella

Campylobacter

Shigella

E. coli

11. The following organisms are considered possible Bioterrorism organism. Check all that apply.

Bacillus anthracis

Staph aureus

Yersinia pestis

Strep pneumo

Francisella tularensis

E. coli

Brucella species

Submit

12. The Oxidase test is useful in initial characterization of gram negative bacteria. Which of the following would be Oxidase positive?

E. coli

Pseudomonas

Campylobacter

Klebsiella

Submit