Microbiology Competency_weeks_2012

1.

The following organisms are considered possible Bioterrorism organism. Check all that apply.

A.

Bacillus anthracis
B.

Staph aureus
C.

Yersinia pestis
D.

Strep pneumo
E.

Francisella tularensis
F.

E. coli
G.

Brucella species

Correct Answer(s)
A. Bacillus anthracis
C. Yersinia pestis
E. Francisella tularensis
G. Brucella species

2.

Universal precautions are only necessary when handling specimens that have contamination on the outside of the container.

A.

True
B.

False

Correct Answer
B. False

3.

Check any of the following that are appropriate reasons for rejecting a specimen for microbiology.

A.

Unlabeled specimen
B.

Leaky container
C.

Wound culture submitted in a culturette containine Modified Ames Stuart media
D.

Urine specimen delayed transport of 4 hours
E.

Properly collected clean catch urine delivered immediately to the lab
F.

A dry swab

Correct Answer(s)
A. Unlabeled specimen
B. Leaky container
D. Urine specimen delayed transport of 4 hours
F. A dry swab

4.

Intracellular gram-negative dipplococci seen on a gram stain of CSF would be suspicious of what organism?

A.

E. coli
B.

Neisseria meningococcus
C.

Haemophilus influenza
D.

Staph aureus

Correct Answer
B. Neisseria meningococcus

5.

Grape-like clusters of gram positive cocci seen on a gram stain would be suspect of which organism?

A.

Staph species
B.

Strep species
C.

Brucella species
D.

Enterobacteriaceae

Correct Answer
A. Staph species

6.

A colorless/clear colony growing on XLD agar should be worked up for which of the following enteric pathogen?

A.

Salmonella
B.

Campylobacter
C.

Shigella
D.

E. coli

Correct Answer
C. Shigella

7.

A black colony found growing on XLD agar should be worked up for which of the following enteric pathogens?

A.

Salmonella
B.

Shigella
C.

Campylobacter
D.

E. coli

Correct Answer
A. Salmonella

8.

A clean catch urine sample is growing >100,000 of a single type gram negative rod. This culture should be worked up as positive and the organism should be identified and sensitivities performed.

A.

True
B.

False

Correct Answer
A. True

9.

A sputum specimen is received and the gram stain is showing >25 squamous epithelials per low power field. What is the appropriate next step?

A.

Set the culture up
B.

Notify the appropriate clinician and request a new specimen
C.

Throw the sample away and don't tell anyone
D.

Go yell at the nurse for sending you spit

Correct Answer
B. Notify the appropriate clinician and request a new specimen

10.

The most common use of the Catalase test is to:

A.

Presumptively identify yeast
B.

Distinguish between Staph and Strep species
C.

To test for Protein A
D.

To test for X and V factors

Correct Answer
B. Distinguish between Staph and Strep species

11.

The Oxidase test is useful in initial characterization of gram negative bacteria. Which of the following would be Oxidase positive?

A.

E. coli
B.

Pseudomonas
C.

Campylobacter
D.

Klebsiella

Correct Answer(s)
B. Pseudomonas
C. Campylobacter

12.

Which statements are true of Beta lactamases? Check all that apply

A.

Beta lactamases are enzymes produced by certain bacteria that are able to inactivate Beta lactam antibiotics (phnicillins and cephalosporins)
B.

Testing for Beta lactamase is not a very useful test
C.

Direct Beta lactamase testing can be used as a rapid and effective method of detecting resistance to beta lactam antibiotics in strains of Staph.aureus, Haemophilus influenza, Neisseria gonorrhea and Moraxella catarrhalis
D.

Beta lactamase test detects indole production

Correct Answer(s)
A. Beta lactamases are enzymes produced by certain bacteria that are able to inactivate Beta lactam antibiotics (phnicillins and cephalosporins)
C. Direct Beta lactamase testing can be used as a rapid and effective method of detecting resistance to beta lactam antibiotics in strains of Staph.aureus, Haemophilus influenza, Neisseria gonorrhea and Moraxella catarrhalis