Medical Laboratory Assistant

The specimens must be spun with a cap on to prevent any leakage or contamination during the spinning process. The cap ensures that the contents of the specimen remain contained and do not spill out, which could affect the accuracy of the results or potentially harm the person handling the specimen.

It is critical to monitor the temperature of certain lab refrigerators in order to maintain the integrity of the products stored. Fluctuations in temperature can have a negative impact on the stability and effectiveness of the products, such as vaccines or sensitive chemicals. By monitoring the temperature, any deviations can be identified and corrected promptly to ensure the quality and efficacy of the stored products.

The temperature of a water bath should be checked once a day and each time it is used to ensure that it is at the desired temperature and remains stable throughout the day. Checking the temperature when filling the water bath may not be sufficient as the temperature can fluctuate over time. By checking it regularly, any deviations or changes can be identified and corrected promptly to maintain the desired temperature for various applications.

The water bath temperatures in the Transfusion Services will be at 37 degrees C when used for ongoing daily work. This temperature is commonly used in laboratories and medical facilities for various purposes, including maintaining the temperature of samples or reagents. It is important to maintain a specific temperature in the water bath to ensure accurate and reliable results in the daily work conducted in the Transfusion Services.

The correct answer is centrifuge because the given cleaning instructions mention wiping the interior to remove blood and pulverized glass, which suggests that the instrument is used for processing biological samples. Centrifuges are commonly used in laboratories to separate components of a mixture based on their density by spinning them at high speeds. Cleaning a centrifuge after a spill or breakage would require wearing gloves, disinfecting the surface, and wiping the interior to remove any contaminants.

The given steps of unplugging the unit, emptying it, wiping down the interior surface with a damp cloth and mild disinfectant solution, and refilling it with purified water to the appropriate level are the proper way to clean water baths.

Micro collection tubes are small tubes used to collect and store small volumes of liquid samples. These tubes are typically designed to fit inside larger test tubes. When placed inside an empty test tube, the micro collection tubes can be easily spun in a centrifuge. Centrifugation is a process that uses high-speed rotation to separate substances of different densities. By spinning the micro collection tubes in a centrifuge, any particles or substances present in the liquid sample can be separated and collected at the bottom of the tubes. Therefore, the statement that micro collection tubes can be spun in centrifuges if they are placed inside an empty test tube is true.

The correct answer is "All of the above" because all the options mentioned in the question are types of centrifuges. A horizontal rotor centrifuge is one where the rotor spins horizontally, while a fixed-angle centrifuge has a fixed angle between the sample and the rotor. Ultra-centrifuges are high-speed centrifuges used for separating particles at very high speeds. Therefore, all the options listed in the question are valid types of centrifuges.

Centrifuges are used to separate components of a blood specimen by spinning it at high speeds. The range of 1000g-3000g indicates the force exerted on the specimen during centrifugation. This range is commonly used in laboratories to ensure proper separation of the blood components without causing damage or hemolysis. Higher forces (such as 2000g-4000g or 3000g-5000g) could potentially lead to the destruction of red blood cells, while lower forces may not provide sufficient separation. Therefore, 1000g-3000g is the correct range for spinning blood specimens in a centrifuge.

Fixed Angles achieve higher RCFs than Horizontal

An acceptable time for a centrifuge to spin blood specimens is typically between 10-15 minutes. This duration allows for the proper separation of blood components, such as plasma and red blood cells, ensuring accurate test results. If the centrifuge spins for less than 10 minutes, the separation may be incomplete, leading to inaccurate results. On the other hand, if it spins for more than 15 minutes, it may cause damage to the blood cells or other components, again affecting the accuracy of the test. Therefore, 10-15 minutes is the recommended time range for spinning blood specimens in a centrifuge.

The correct answer is the plastic or rubber mat on the bottom of each cup in the centrifuge. A bung is a small stopper or plug that is used to seal the opening of a container, in this case, the cup in the centrifuge. It is typically made of plastic or rubber and is placed at the bottom of each cup to prevent any leakage or spillage during the centrifugation process.

The correct answer is "wiped down with a disinfectant solution." When cleaning the interior of an incubator, it is important to use a disinfectant solution to eliminate any potential contaminants. Wiping down the interior surfaces helps to ensure that the disinfectant is evenly distributed and effectively kills any harmful microorganisms. This method is commonly used in healthcare settings to maintain a clean and sterile environment.

glasware is rinsed 3 times with tap water then 3 times with purified water ( usuallt deionized water)

MLAs most often deal with electrical equipment such as fridges/freezers, centrifuges, incubators, and water baths. These are commonly used in various scientific and medical settings. Fridges and freezers are essential for storing samples and specimens at low temperatures. Centrifuges are used to separate substances based on their density. Incubators provide controlled environments for the growth and development of cells or organisms. Water baths are used for heating or maintaining constant temperatures for experiments or sample preparation.

The given answer lists the types of air incubators as normal (aerobic) incubator, anaerobic chamber, and CO2 incubator. A normal (aerobic) incubator is used for growing bacteria that require oxygen, while an anaerobic chamber is used for growing bacteria that cannot survive in the presence of oxygen. A CO2 incubator is used for growing cells that require a specific level of carbon dioxide. These three types of incubators cater to different growth requirements of microorganisms and cells.

Purified water should be used in a water bath because it is free from impurities and contaminants. Tap water may contain minerals and chemicals that can affect the experiment or reaction being conducted. Boiled water may still contain some impurities. Distilled water is a better option but may still contain trace amounts of impurities. Purified water, on the other hand, has undergone additional filtration processes to remove impurities, making it the most suitable choice for a water bath.

If the centrifuge is not properly loaded and balanced, it can cause undue wear on the rotor. This is because an unbalanced load can create uneven forces on the rotor, leading to increased friction and wear. Additionally, it can cause excessive vibration which could lead the centrifuge to move off the countertop. This is because the unbalanced load creates uneven forces that can cause the centrifuge to shake and potentially move. Lastly, an unbalanced tube can break, as the uneven forces can cause stress and strain on the tube, leading to its failure.

The departments of hematology, chemistry, and histopathology may also use a water bath. This suggests that these departments require a water bath for their respective processes or experiments.

The correct answer is inside a biological safety cabinet or a fume hood because centrifuges are used to spin samples at high speeds, which can generate aerosols or release harmful substances. Placing the centrifuge inside a biological safety cabinet or a fume hood helps to contain these aerosols or substances, ensuring the safety of the operator and preventing contamination of the surrounding environment. Placing the centrifuge on the counter top does not provide the same level of containment and safety.

The glassware should be inspected to examine the surface and ensure that it is clean and free from any contaminants. Once wet with purified water, the surface should be coated with a film of water, indicating that it is properly rinsed. There should be no beads or oily patches where the water does not uniformly coat the glassware surface, as this would suggest inadequate rinsing and the presence of residues.

The primary purpose of a centrifuge is to separate the serum or plasma from the cellular portion of the blood. This is achieved by spinning the blood sample at high speeds, causing the components to separate based on their density. The heavier cellular portion settles at the bottom, while the lighter serum or plasma collects on top. This separation allows for further analysis or testing of the specific component of interest.