Micro Practicum

The correct answer refers to a milk plate used to test for the enzyme caseinase where a positive result is the formation of a the clearing zone.

The Citrate test is negative when there is no utilization of Citrate, indicating that Citrate is the sole carbon source being tested for. Testing for Nitrate instead would be a different procedure altogether, resulting in different outcomes.

The correct answer describes the results of the pigment test with the organism M. roseus on NA slant. The incorrect answers provide alternative scenarios that are different from the actual test results.

The correct answer refers to a carbohydrate fermentation test that determines the fermentation of sugars resulting in acid production. The incorrect answers do not align with the testing process or expected results of the given scenario.

The correct answer is MSA plate which specifically tests for halophiles and mannitol fermentation. The plate is both differential and selective, containing a pH indicator (Phenol Red) to detect mannitol fermentation. Organism SA is a halophile and mannitol fermenter, organism SP is a halophile but does not ferment mannitol, and organism EC is not a halophile.

The Lys Test specifically tests for Lysine Decarboxylase enzyme. In this case, the tube is negative for the enzyme as indicated by the correct answer.

A positive citrate test indicates that the bacteria is capable of utilizing citrate as a sole source of carbon and energy. This is commonly used to differentiate between different species of bacteria based on their metabolic capabilities.

Barritt's reagent A is used to test for the presence of acetoin production in non acidic fermentation. When the reagent gives a red color, it is considered positive for acetoin production.

The correct answer describes the production of alkaline and ammonia on the left tube, leading to an increase in pH due to no fermentation. The incorrect answers provide variations in the outcomes, explaining the different possibilities of results in the given scenario.

Catalase test is commonly used to determine the presence of the enzyme catalase in bacteria, which breaks down hydrogen peroxide into water and oxygen. Other enzymes, such as amylase, lipase, and protease, are not involved in this specific test.

The correct answer describes a positive result for carbohydrate fermentation, acid, and gas production, indicating the presence of certain metabolites. The incorrect answers provide alternative scenarios that do not align with the specific processes being tested in this context.

The question is about identifying a specific test, media used, and the result obtained. The correct answer provides the test (H2S test), the media used (Peptone iron deep), and the positive result for cystein desulfurase. The incorrect answers provide misleading information about other tests and results to test the respondent's understanding.

In streptomycin testing, line A typically represents the killing dose of streptomycin, where most colonies are eliminated. Line B represents highly resistant colonies that survive even at high concentrations of streptomycin.

The test in question is likely a biochemical test used to identify the presence of specific decarboxylase enzymes in a bacterial isolate. The correct answer, + lys decarboxylase, indicates a positive result for lysine decarboxylase activity. The incorrect answers - arginine decarboxylase, - ornithine decarboxylase, and - tyrosine decarboxylase represent negative results for the decarboxylase activities of arginine, ornithine, and tyrosine respectively.

The Phe test is specifically used for testing for phe aminase, which produces a green color in a positive reaction.

The urease test is used to determine the presence of the urease enzyme. It involves using a Urea slant as the media. A positive result indicates the presence of urease, which converts urea to ammonia and carbon dioxide, leading to an alkaline pH change in the media.

In the IMViC test, Indole test uses Kovac's reagent to test for the presence of tryptophanase enzyme which breaks down tryptophan into indole, pyruvic acid, and ammonia. This reaction results in the formation of a red color if the test is positive.

The correct answer refers to an EMB Plate which tests for Gram-negative or Gram-positive bacteria. Gram-negative bacteria exhibit strong growth, while Gram-positive bacteria exhibit weak growth. Positive lactose fermentation results in a dark colony with a metallic sheen, while negative lactose fermentation results in a pale colony.

PEA plates are selective media used to isolate Gram-positive organisms, inhibiting the growth of Gram-negative bacteria.

The correct test being performed is the Gelatin test, not Amylase, Lipase, or Urease test. It tests for gelatinase, not amylase, lipase, or urease. The result of A is positive (+) for gelatinase, not amylase, lipase, or urease.

Testing motility involves assessing the ability of microorganisms to move. Soft agar stab inoculation and hanging drop technique are specifically designed methods for testing motility, different from the incorrect options provided.

The correct components of the FTB tube are Thioglycollate, Resazurin, and Gelatin. These tubes are used for determining oxygen preferences of bacteria.

Enterobacteriaceae are a family of Gram-negative, oxidase-negative, facultatively anaerobic, rod-shaped bacteria that typically ferment glucose producing acid.

The correct answer is basic as it is commonly used in simple stains like methylene blue or crystal violet stain and acid-fast stain like Ziehl-Neelsen stain. Acidic stains, on the other hand, are used in techniques like Gram staining or Giemsa stain.

A negative stain technique uses acidic dyes that are repelled by the bacterial cell, resulting in a colorless cell against a colored background.

Calcofluor White is a fluorescent dye that is commonly used for staining fungi in microbiology. It binds specifically to chitin, a structural component found in the cell walls of fungi. The dye illuminates the presence of fungi under a microscope, making it easier to identify and study them.

The correct answer describes a diagnostic plastic card used specifically for conducting RPR tests in a lab setting to screen for STDs.

A positive VP test indicates the production of acetoin through non-acidic fermentation.

Nitrogen free mannitol agar is not a general growth medium, it specifically selects for nitrogen fixers. The growth on it is typically clear and slimy due to the presence of certain bacteria.