Quiz: How Good Are You at Using a Micropipette?

A micropipette is used to accurately measure and transfer small volumes of liquid, typically ranging from 0.1 μL to 1000 μL. It is commonly used in laboratories for various scientific and research purposes.

Vertical angle pipetting is the recommended technique for ensuring accuracy while pipetting. Holding the micropipette vertically and at a slight angle, touching the inner wall of the receiving vessel, helps prevent sample loss, air bubbles, and ensures precise measurement.

A P200 micropipette is designed to measure a maximum volume of 200 μL. It is commonly used for medium volume transfers and is suitable for applications that require precise and accurate pipetting within this range.

The volume adjustment dial is the part of a micropipette that allows the user to select the desired volume. By turning the dial, the user can set the micropipette to aspirate and dispense the required volume with high precision.

If liquid comes in contact with the volume adjustment dial, it should be immediately cleaned and dried. Liquid on the dial may affect the accuracy of the micropipette's volume setting and potentially cause malfunction. Cleaning ensures reliable and precise measurements can be made.

A micropipette should be stored standing upright when not in use. This prevents any liquid inside the micropipette from flowing back into the internal mechanism, which could cause damage or malfunction. Additionally, it helps maintain the cleanliness and integrity of the micropipette.

The plunger in a micropipette is used to release the liquid once the desired volume has been aspirated. By pressing the plunger, the liquid is dispensed accurately and precisely, allowing for controlled transfer of the liquid to the desired location.

A new micropipette tip should be used after transferring a large volume, when changing to a different liquid or sample, or if the micropipette has been dropped or mishandled. Using a new tip in these situations ensures accuracy, prevents cross-contamination, and maintains the integrity of the samples being handled.

Maintenance practices for micropipettes do not include regularly lubricating the plunger, storing micropipettes in direct sunlight, autoclaving with the tip attached, or using harsh chemicals for cleaning. These practices can lead to damage, inaccurate measurements, or contamination. Proper handling, routine calibration, and cleaning with mild solutions are recommended instead.

If a micropipette tip is stuck or difficult to eject, one should tap the side of the micropipette gently on a hard surface. This helps dislodge the tip and allows for easy ejection without the risk of damaging the micropipette or creating an aerosol of the sample droplet.