Focus on the Facts: The Resolution Magnification Quiz

If magnification only makes an object look larger without adding detail, and if the goal is to see two separate points clearly, then the term must be resolution.

If total magnification is calculated by multiplying the ocular lens power by the objective lens power, then 10 multiplied by 40 equals 400x.

If magnification only increases the size of the image and resolution depends on the quality and wavelength of light, then increasing size does not automatically mean the image will be sharper or clearer.

If you zoom in closer to a specific part of a specimen (increase magnification), then the total area you can see (field of view) must decrease.

If the ocular lens has a fixed low power and the objective lenses are interchangeable with higher powers, then the objective lens is the primary source of high magnification.

If an image is large (high magnification) but you cannot distinguish separate parts (blurry), then the resolution is insufficient for that size.

If the user looks through the eyepiece and sees a circular lighted area containing the specimen, then that defined area is called the field of view.

If resolution is defined by the minimum distance between two points that can still be seen as separate, then a smaller distance value indicates the ability to see finer detail.

If light bends (refracts) when moving from glass to air, and if oil has the same refractive index as glass, then using oil prevents light loss and increases resolution.

If resolution is determined by the physics of light waves and lens quality, then wavelength, aperture, and lens material are relevant, while the stand color and table height are not.

If the 4x objective has a large working distance, then the coarse adjustment is used first to quickly bring the specimen into general focus without hitting the lens.

If an image appears 100 times larger than the physical specimen, then the ratio describing this scale change is known as magnification.

If shorter wavelengths allow for the distinction of smaller structures, and if electrons have significantly shorter wavelengths than light, then electron microscopes provide superior resolution.

If the specimen is too bright or too dim, then adjusting the diaphragm will change the aperture size to regulate light flow.

If the light path must be continuous from the source to the eye, and if the lens is between settings, then the light will be blocked, resulting in a dark view.

If a microscope is designed so that the focal planes of all objectives are the same, then switching lenses will require only minimal fine-tuning of the focus.

If light travels in waves and these waves diffract (spread out) when passing through an opening, then there is a physical limit to how close two points can be before their diffraction patterns overlap.

If magnification is the multiplier for size, then 0.1 mm multiplied by 100 equals 10 mm.

If electron microscopes are categorized by how they interact with the specimen (surface scanning vs. passing through), then SEM and TEM are the correct classifications.

If a higher power lens has a smaller aperture, then less light is gathered from the specimen, resulting in a dimmer image that requires light adjustment.