Microbiology Laboratory Techniques and Safety Quiz

The decolorizer used in the Gram staining process is Acetone-Alcohol, which helps to decolorize certain types of bacteria while retaining color in others.

In Gram staining, safranin is used as the counter stain after the initial staining with crystal violet and iodine. Safranin imparts a red or pink color to the gram-negative bacteria, allowing them to be differentiated from the gram-positive bacteria which retain the crystal violet-iodine complex.

Spirillium is a type of bacteria characterized by its spiral shape. It is distinct from other organisms such as Euglena, Paramecium, and Amoeba, which belong to different biological classifications.

The Capsule stain procedure uses crystal violet as the primary stain to stain the background and the capsule itself while staining the cell with copper sulfate or neutral red.

In a gram stain, bacteria are classified based on their cell wall composition. Gram negative bacilli are characterized by appearing pink or red under a microscope after staining with certain dyes.

In a gram stain, bacteria are categorized as either gram positive or gram negative based on their cell wall composition. Streptococcus is a gram positive bacteria that appears purple under the microscope after the staining process. Escherichia coli and Pseudomonas aeruginosa are gram negative bacteria, while Staphylococcus aureus is another example of a gram positive bacterium.

The correct answer is Acid-fast stain with Acid Alcohol as the decolorizer. Acid-fast Bacilli will retain the stain while Acid-negative Staphylococcus will lose the stain during the decolorization process.

The Push-slide technique is commonly used in the Negative stain and Capsule stain procedures, making it a unique method for observing specific characteristics of bacterial cells.

Negative staining is a technique used in microbiology to observe capsules, flagella, and other structures that are easily distorted or destroyed by heat fixing. Unlike negative staining, the other staining procedures mentioned would typically involve heat fixing the sample for improved visualization.

The correct answer is 'Endospore forming bacillus' because the slide shows a bacterium that is capable of forming endospores. Gram-positive cocci, anaerobic virus, and fungal hyphae are all different types of microorganisms that do not match the description of the bacterium on the slide.