Mastering Microscope Techniques and Applications Quiz

1. When using a microscope, what is the relationship between the magnification and the condenser setting?

Lower

Unaffected

Higher

Fluctuates

When the magnification on the microscope is increased, the condenser setting also needs to be adjusted to a higher value to optimize the image quality. This ensures that sufficient light is focused on the sample at higher magnifications.

Explanation

When the magnification on the microscope is increased, the condenser setting also needs to be adjusted to a higher value to optimize the image quality. This ensures that sufficient light is focused on the sample at higher magnifications.

2. How should the condensor be positioned when storing a microscope?

It doesn't matter, any position is fine

In the lowest position

In the highest position

In the middle position

Storing the condensor in the highest or middle position can lead to potential damage. It is important to always store the condensor in the lowest position to ensure the safety and longevity of the microscope.

Explanation

Storing the condensor in the highest or middle position can lead to potential damage. It is important to always store the condensor in the lowest position to ensure the safety and longevity of the microscope.

3. What could be the issue if you can clearly see a specimen on low power objective but can't see or focus on the high or oil objective?

The microscope is faulty and needs to be replaced.

The specimen is too small to be visible under high or oil objectives.

The lighting source is not functioning properly.

The issue of not being able to see or focus on the high or oil objective is commonly due to the slide being loaded incorrectly. By turning the slide over and reloading it properly, the problem can be resolved without needing to replace the microscope or making adjustments to specimen size or lighting source.

Explanation

The issue of not being able to see or focus on the high or oil objective is commonly due to the slide being loaded incorrectly. By turning the slide over and reloading it properly, the problem can be resolved without needing to replace the microscope or making adjustments to specimen size or lighting source.

4. How is a refractometer calibrated?

It is calibrated by adding a drop of oil to the glass cover and adjusting the refractometer reading.

It is calibrated by exposing the refractometer to sunlight for a few hours.

It is calibrated by using tap water at room temperature.

Calibrating a refractometer involves using distilled water at a specific temperature range to set the device to zero. This ensures accurate readings for measuring the refractive index of liquids.

Explanation

Calibrating a refractometer involves using distilled water at a specific temperature range to set the device to zero. This ensures accurate readings for measuring the refractive index of liquids.

5. What are the three layers of a spun hematocrit tube?

RBC, plasma/serum, buffy coat

Platelets, plasma/serum, WBC

WBC, buffy coat, plasma/serum

Plasma/serum, buffy coat (WBC and platelets), RBC

In a spun hematocrit tube, the layers are arranged in the order of plasma/serum, buffy coat (WBC and platelets), and RBC from top to bottom.

Explanation

In a spun hematocrit tube, the layers are arranged in the order of plasma/serum, buffy coat (WBC and platelets), and RBC from top to bottom.

6. What are the normal values for a feline(PCV, TP, HGB, RBC and WBC)?

B) PCV- 40-50% (45%) TP- 7.5-9.0 g/dL HGB- 15-18 g/dL RBC- 7.0-8.0 x 10(6)/mm(3) WBC- 12.0-24.0 x 10(3)/mm(3).

A) PCV- 20-30% (25%) TP- 5.0-6.0 g/dL HGB- 10-12 g/dL RBC- 5.0-6.0 x 10(6)/mm(3) WBC- 3.0-10.0 x 10(3)/mm(3).

PCV- 30-45% (37%)TP- 6.0-7.5 g/dLHGB- 12-15 g/dLRBC- 6.0-7.0 x 10(6)/mm(3) WBC- 5.5-19.5 x 10(3)/mm(3)

C) PCV- 25-35% (30%) TP- 5.5-7.0 g/dL HGB- 11-14 g/dL RBC- 5.5-6.5 x 10(6)/mm(3) WBC- 4.0-15.0 x 10(3)/mm(3).

The correct normal values for a feline are PCV- 30-45% (37%) TP- 6.0-7.5 g/dL HGB- 12-15 g/dL RBC- 6.0-7.0 x 10(6)/mm(3) WBC- 5.5-19.5 x 10(3)/mm(3). Option A has lower values, option B has higher values, and option C has slightly lower values compared to the correct answer.

Explanation

The correct normal values for a feline are PCV- 30-45% (37%) TP- 6.0-7.5 g/dL HGB- 12-15 g/dL RBC- 6.0-7.0 x 10(6)/mm(3) WBC- 5.5-19.5 x 10(3)/mm(3). Option A has lower values, option B has higher values, and option C has slightly lower values compared to the correct answer.

7. What are the normal values for a canine PCV, TP, HGB, RBC, and WBC?

PCV- 30-40%, TP- 6.5-8.0 g/dL, HGB- 11-14 g/dL, RBC- 7.0-8.0 x 10(6)/mm(3), WBC- 15.0-20.0 x 10(3)/mm(3)

PCV- 45-65%, TP- 7.0-8.5 g/dL, HGB- 10-12 g/dL, RBC- 5.0-6.0 x 10(6)/mm(3), WBC- 5.0-10.0 x 10(3)/mm(3)

PCV- 37-55% (45%)TP- 6.0-7.5 g/dLHGB- 12-15 g/dLRBC- 6.0-7.0 x 10(6)/mm(3)WBC- 6.0-15.0 x 10(3)/mm(3)

PCV- 20-30%, TP- 5.0-6.0 g/dL, HGB- 8-10 g/dL, RBC- 3.0-4.0 x 10(6)/mm(3), WBC- 10.0-20.0 x 10(3)/mm

8. What information can you obtain from a spun PCV?

PCV, TP, buffy coat, plasma color and microfalaria

Blood sugar levels, platelet count, lipid profile and electrolyte levels

White blood cell differential count, erythrocyte sedimentation rate, coagulation profile and serum protein levels

Hematocrit count, RBC count, WBC count and plasma volume

When a PCV is spun, it separates into different components that provide valuable information about the blood sample. The correct answer includes components like PCV, TP, buffy coat, plasma color, and microfalaria. The incorrect answers mention unrelated blood parameters that are not obtained specifically from a spun PCV.

Explanation

When a PCV is spun, it separates into different components that provide valuable information about the blood sample. The correct answer includes components like PCV, TP, buffy coat, plasma color, and microfalaria. The incorrect answers mention unrelated blood parameters that are not obtained specifically from a spun PCV.