Quiz Review Biologi Molekuler 2009/2010 (I)
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Quiz Review Biologi Molekuler 2009/2010 (() Program Studi Biologi (S1) Fakultas Biologi UNSOED ini mencakup materi pendahuluan sampai dengan transkripsi. Pada hari Rabu, 28 April 2010, Jam 10.00 - 22.00 WIB, Quiz ini dapat diakses tanpa password.
- 1.The nucleic acids DNA and RNA are polymers of ___________
- A.
Amino acids
- B.
Nucleotides
- C.
Nucleic acids
- D.
Glucose
- E.
Nucleosides
- 2.Polysaccharides are polymers of simple sugars covalently linked by ___________
- A.
Peptide bonds
- B.
Hydrogen bonds
- C.
Glycosidic bonds
- D.
Phosphodiester bond
- 3.In nucleic acids, the bond between the bases and the sugars is _________
- A.
Peptide bonds
- B.
Glycosylic (or glycosidic) bond
- C.
Phosphodiester bond
- D.
Hydrogen bonds
- 4.In a DNA or RNA molecule, deoxyribonucleotides or ribonucleotides respectively are joined into a polymer by the covalent linkage of a phosphate group between the 5-hydroxyl of one ribose and the 3-hydroxyl of the next.This kind of bond or linkage is called ____________
- A.
Peptide bonds
- B.
Glycosylic (or glycosidic) bond
- C.
Phosphodiester bond
- D.
Hydrogen bonds
- 5.There is a convention to write the sequences of DNA or RNA with the 5-end at the right.
- A.
TRUE
- B.
FALSE
- 6.In strong acid and at elevated temperatures, for example perchloric acid (HClO4) at more than 100°C, nucleic acids are hydrolyzed completely to their constituent
- A.
TRUE
- B.
FALSE
- 7.Chemical agents can cause the denaturation of DNA or neutral pH.
- A.
Urea and ehidium bromide
- B.
Urea and formamide
- C.
Formamide and perchloric acid
- D.
Ehidium bromide and perchloric acid
- E.
Ehidium bromide and cesium chloride
- 8.The absorbance at 260 nm is used to determine the __________ of nucleic acids.
- A.
Concentration
- B.
Purity
- C.
Double-stranded DNA
- D.
Single-stranded DNA
- 9.The approximate purity of dsDNA preparations may be estimated by determination of the ratio of absorbance at 260 and 280 nm (A260/A280).
- A.
TRUE
- B.
FALSE
- 10.The pure dsDNA has an A260/A280 of ____
- A.
2.0
- B.
0.18
- C.
1.8
- D.
18
- 11.The pure RNA has an A260/A280 of ______
- A.
2.0
- B.
0.18
- C.
1.8
- D.
18
- 12.The DNA in E. coli cells consists of a single closed-circular DNA molecule of length 4.6 million base pairs. The DNA is packaged into a region of the cell known as _________
- A.
The nucleoid
- B.
The nucleus
- C.
The nucleolus
- D.
Tjhe nicleosome
- E.
The nucleoplasm
- 13.The major protein components of chromatin are the histones; small, basic (positively charged) proteins which bind tightly to DNA. There are four families of core histone:______________
- A.
H2A, H2B, H3, H4
- B.
H2A, H2B, H3, H1
- C.
H2A, H2B, H1, H4
- D.
H1, H2B, H3, H4
- 14.Chromatin is organized into a larger structure, known as the ____________
- A.
Nucleosome
- B.
Chromatosome
- C.
Solenoid
- D.
Histone
- 15.A nucleosome core plus H1 is known as a _________________
- A.
Chromatosome
- B.
Chromatin
- C.
Chromosome
- D.
Loops
- E.
Solenoid
- 16.Chromosomes greatly alter their level of compactness as cells progress through the cell cycle, varying between highly condensed chromosomes at _________ and very much more diffuse structures in _______
- A.
Anaphase, interphase
- B.
Metaphase, prophase
- C.
Prophase, interphase
- D.
Metaphase, interphase
- 17.The __________ is the constricted region where the two sister chromatids are joined in the metaphase chromosome.
- A.
Centromere
- B.
Telomere
- C.
Kinetochore
- D.
Microtubules
- E.
Heterochromatin
- 18.Specialized DNA sequences that form the ends of the linear DNA molecules of the eukaryotic chromosomes
- A.
Kinetochore
- B.
Microtubules
- C.
Centromere
- D.
Telomeres
- 19.This enzyme has been used to map the regions of transcriptionally active chromatin in cells.
- A.
The nuclease deoxyribonuclease I (DNase I)
- B.
DNA polymerase III
- C.
DNA polymerase I
- D.
DNA helicase
- E.
DNA primase
- 20.Enzymes which use the energy of ATP hydrolysis to move into and melt double-stranded DNA (or RNA).
- A.
DNA primase
- B.
DNA polymerase III
- C.
DNA helicase
- D.
DNA polymerase I
- E.
DNA ligase
- 21.The single-stranded bubble created by DNA helicase is coated with _______________ to protect it from breakage and to prevent the DNA renaturing.
- A.
DNA helicase
- B.
RNA primer
- C.
DNA polymerase I
- D.
DNA ligase
- E.
Singlestranded binding protein (Ssb)_
- 22.The enzyme ____________ attaches to the DNA and synthesizes a short RNA primer to initiate synthesis of the leading strand of the first replication fork.
- A.
DNA polymerase I
- B.
DNA primase
- C.
DNA ligase
- D.
DNA polymerase III
- E.
Singlestranded binding protein (Ssb)_
- 23.Inhibitors of , such as novobiocin and oxolinic acid, are effective inhibitors of bacterial replication and have antibiotic activity.
- A.
TRUE
- B.
FALSE
- 24.DNA primase synthesizes RNA primers every 1000–2000 nt on the lagging strand.
- A.
TRUE
- B.
FALSE
- 25.Both leading and lagging strand primers are elongated by ______
- A.
DNA polymerase III
- B.
DNA primase
- C.
DNA gyrase
- D.
DNA polymerase I
- E.
DNA ligase
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