1.
The nucleic acids DNA
and RNA are polymers of ___________
A. 
B. 
C. 
D. 
E. 
2.
Polysaccharides are
polymers of simple sugars covalently linked by ___________
A. 
B. 
C. 
D. 
3.
In nucleic acids, the
bond between the bases and the sugars is _________
A. 
B. 
Glycosylic (or glycosidic) bond
C. 
D. 
4.
In a DNA or RNA molecule,
deoxyribonucleotides or ribonucleotides respectively are joined into a
polymer by the covalent linkage of a phosphate group between the 5-hydroxyl of one ribose and the 3-hydroxyl of the next.This kind of bond or linkage is called ____________
A. 
B. 
Glycosylic (or glycosidic) bond
C. 
D. 
5.
There is a convention to write the sequences of DNA or RNA with
the 5-end at the right.
6.
In strong acid and at
elevated temperatures, for example perchloric acid (HClO4) at more than 100°C,
nucleic acids are hydrolyzed completely to their constituent
7.
Chemical agents can cause the denaturation of
DNA or
neutral pH.
A. 
B. 
C. 
Formamide and perchloric acid
D. 
Ehidium bromide and perchloric acid
E. 
Ehidium bromide and cesium chloride
8.
The absorbance at 260
nm is used to determine the __________ of nucleic acids.
A. 
B. 
C. 
D. 
9.
The approximate
purity of dsDNA preparations may be estimated by determination of the ratio of
absorbance at 260 and 280 nm (A260/A280).
10.
The pure dsDNA has an
A260/A280 of ____
A. 
B. 
C. 
D. 
11.
The pure RNA has an
A260/A280 of ______
A. 
B. 
C. 
D. 
12.
The DNA in E. coli
cells consists of a single closed-circular DNA molecule of length 4.6 million
base pairs. The DNA is packaged into a region of the cell known as _________
A. 
B. 
C. 
D. 
E. 
13.
The major protein
components of chromatin are the histones; small, basic (positively charged)
proteins which bind tightly to DNA. There are four families of core histone:______________
A. 
B. 
C. 
D. 
14.
Chromatin is
organized into a larger structure, known as the ____________
A. 
B. 
C. 
D. 
15.
A nucleosome core plus H1 is known as a _________________
A. 
B. 
C. 
D. 
E. 
16.
Chromosomes greatly
alter their level of compactness as cells progress through the cell cycle,
varying between highly condensed chromosomes at _________ and very much more
diffuse structures in _______
A. 
B. 
C. 
D. 
17.
The __________ is the
constricted region where the two sister chromatids are joined in the metaphase
chromosome.
A. 
B. 
C. 
D. 
E. 
18.
Specialized DNA
sequences that form the ends of the linear DNA molecules of the eukaryotic
chromosomes
A. 
B. 
C. 
D. 
19.
This enzyme has been used to map
the regions of transcriptionally active chromatin in cells.
A. 
The nuclease deoxyribonuclease I (DNase I)
B. 
C. 
D. 
E. 
20.
Enzymes which use the
energy of ATP hydrolysis to move into and melt double-stranded DNA (or RNA).
A. 
B. 
C. 
D. 
E. 
21.
The single-stranded
bubble created by DNA helicase is coated with _______________
to protect it from breakage and to prevent the DNA renaturing.
A. 
B. 
C. 
D. 
E. 
Singlestranded binding protein (Ssb)_
22.
The enzyme ____________ attaches to the DNA and synthesizes a short RNA primer to initiate
synthesis of the leading strand of the first replication fork.
A. 
B. 
C. 
D. 
E. 
Singlestranded binding protein (Ssb)_
23.
Inhibitors of , such as novobiocin and oxolinic acid, are effective inhibitors of
bacterial replication and have antibiotic activity.
24.
DNA primase synthesizes RNA primers every
1000–2000 nt on the lagging strand.
25.
Both leading and
lagging strand primers are elongated by ______
A. 
B. 
C. 
D. 
E.