Quiz Online 4 Chapter 5 Host Cells And Vectors

28 Questions | Total Attempts: 2679

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Quiz Online 4 Chapter 5 Host Cells And Vectors - Quiz

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Questions and Answers
  • 1. 
    Insertion of a fragment of DNA, containing a gene, into a cloning vector, and subsequent propagation of the recombinant DNA molecule in a host organism.
    • A. 

      Gene cloning

    • B. 

      Recombinant DNA

    • C. 

      Cloning vector

    • D. 

      Multiple cloning site

  • 2. 
    A DNA molecule created in the test tube by ligating pieces of DNA that are not normally joined together.
    • A. 

      Gene cloning

    • B. 

      Recombinant DNA

    • C. 

      Cloning vector

    • D. 

      Multiple cloning site

  • 3. 
    A cell that harbors foreign molecules, viruses, or microorganisms.
    • A. 

      Gene cloning

    • B. 

      E. coli

    • C. 

      Cloning vector

    • D. 

      Host

  • 4. 
    A vector that can replicate in the cells of more than one organism (e.g. in Escherichia coli and in yeast).
    • A. 

      Shuttle vector

    • B. 

      Insertion vector

    • C. 

      Cloning vector

    • D. 

      Replacement vector

  • 5. 
    A λ vector constructed by deleting a segment of non-essential DNA
    • A. 

      Shuttle vector

    • B. 

      Insertion vector

    • C. 

      Cloning vector

    • D. 

      Replacement vector

  • 6. 
    A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA.
    • A. 

      Shuttle vector

    • B. 

      Insertion vector

    • C. 

      Cloning vector

    • D. 

      Replacement vector

  • 7. 
    A λ vector designed so that insertion of new DNA is by replacement of part of the non-essential region of the λ DNA molecule.
    • A. 

      Shuttle vector

    • B. 

      Insertion vector

    • C. 

      Cloning vector

    • D. 

      Replacement vector

  • 8. 
    A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector.
    • A. 

      Origin of replication

    • B. 

      Selectable marker

    • C. 

      Copy number

    • D. 

      Multiple cloning site

  • 9. 
    A short segment of DNA that contains several unique restriction endonuclease sites in cloning vector
    • A. 

      Origin of replication

    • B. 

      Selectable marker

    • C. 

      Copy number

    • D. 

      Multiple cloning site

  • 10. 
    A usually circular piece of DNA often found in bacteria and some other types of cell.
    • A. 

      Plasmid

    • B. 

      Phage

    • C. 

      Cosmid

    • D. 

      Phagemid

  • 11. 
    Cloning vector comprising a mixture of plasmid and phage DNA.
    • A. 

      Plasmid

    • B. 

      Phage

    • C. 

      Cosmid

    • D. 

      Phagemid

  • 12. 
    A virus that infects a bacterium.
    • A. 

      Plasmid

    • B. 

      Phage

    • C. 

      Cosmid

    • D. 

      Phagemid

  • 13. 
    A high-capacity cloning vector consisting of the λ cos site inserted into a plasmid.
    • A. 

      Plasmid

    • B. 

      Phage

    • C. 

      Cosmid

    • D. 

      Phagemid

  • 14. 
    A cell that has become transformed by the uptake of naked DNA.
    • A. 

      Genomic library

    • B. 

      Transformant

    • C. 

      Host

    • D. 

      Plaque

  • 15. 
    A zone of clearing on a lawn of bacteria caused by lysis of the cells by infecting bacteriophages
    • A. 

      Genomic library

    • B. 

      Transformant

    • C. 

      Host

    • D. 

      Plaque

  • 16. 
    A collection of clones made from a set of randomly generated overlapping DNA fragments representing the entire genome of an organism.
    • A. 

      Genomic library

    • B. 

      Transformant

    • C. 

      Host

    • D. 

      Plaque

  • 17. 
    The acquisition by a cell of new genes by the uptake of naked DNA.
    • A. 

      Insertional inactivation

    • B. 

      Transformation

    • C. 

      Transgenic

    • D. 

      Transfection

  • 18. 
    The introduction of purified phage DNA molecules into a bacterial cell.
    • A. 

      Insertional inactivation

    • B. 

      Transformation

    • C. 

      Transgenic

    • D. 

      Transfection

  • 19. 
    A cloning strategy whereby insertion of a new piece of DNA into a vector inactivates a gene carried by the vector.
    • A. 

      Insertional inactivation

    • B. 

      Transformation

    • C. 

      Transduction

    • D. 

      Transfection

  • 20. 
    A high-capacity cloning vector based on the F plasmid of Escherichia coli.
    • A. 

      YAC

    • B. 

      BAC

    • C. 

      YEp

    • D. 

      YIp

  • 21. 
    This plasmid vectors replicate autonomously because of the presence of a segment of the yeast 2 mm plasmid that serves as an origin of replication (2 mm ori)
    • A. 

      YCp

    • B. 

      BAC

    • C. 

      YEp

    • D. 

      YIp

  • 22. 
    This plasmid vectors do not replicate autonomously, but integrate into the genome at low frequencies by homologous recombination.
    • A. 

      YCp

    • B. 

      BAC

    • C. 

      YEp

    • D. 

      YIp

  • 23. 
    This plasmid vectors are autonomously replicating vectors containing centromere sequences, CEN, and autonomously replicating sequences, ARS.
    • A. 

      YCp

    • B. 

      BAC

    • C. 

      YEp

    • D. 

      YIp

  • 24. 
    The process of using a glass micropipette to insert substances at a microscopic or borderline macroscopic level into a single living cell.
    • A. 

      Microinjection

    • B. 

      Biolistic

    • C. 

      Transformation

    • D. 

      Electroporation

  • 25. 
    A means of introducing DNA into cells that involves bombardment with high-velocity microprojectiles coated with DNA.
    • A. 

      Microinjection

    • B. 

      Biolistic

    • C. 

      Transformation

    • D. 

      Electroporation

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