1.
Insertion
of a fragment of DNA, containing a gene, into a cloning vector, and subsequent
propagation of the recombinant DNA molecule in a host organism.
A. 
B. 
C. 
D. 
2.
A DNA molecule created in the
test tube by ligating pieces of DNA that are not normally joined together.
A. 
B. 
C. 
D. 
3.
A cell that harbors foreign molecules, viruses, or microorganisms.
A. 
B. 
C. 
D. 
4.
A vector that can replicate in the cells of more than one organism (e.g. in Escherichia coli and in yeast).
A. 
B. 
C. 
D. 
5.
A λ vector constructed by
deleting a segment of non-essential DNA
A. 
B. 
C. 
D. 
6.
A DNA molecule that is able to
replicate inside a host cell and therefore can be used to clone other fragments
of DNA.
A. 
B. 
C. 
D. 
7.
A λ
vector designed so that insertion of new DNA is by replacement of part of the
non-essential region of the λ DNA molecule.
A. 
B. 
C. 
D. 
8.
A gene
carried by a vector and conferring a recognizable characteristic on a cell
containing the vector or a recombinant DNA molecule derived from the vector.
A. 
B. 
C. 
D. 
9.
A
short segment of DNA that contains several unique restriction endonuclease
sites in cloning vector
A. 
B. 
C. 
D. 
10.
A
usually circular piece of DNA often found in bacteria and some other types of
cell.
A. 
B. 
C. 
D. 
11.
Cloning
vector comprising a mixture of plasmid and phage DNA.
A. 
B. 
C. 
D. 
12.
A virus that infects a bacterium.
A. 
B. 
C. 
D. 
13.
A
high-capacity cloning vector consisting of the λ cos site inserted into a
plasmid.
A. 
B. 
C. 
D. 
14.
A cell
that has become transformed by the uptake of naked DNA.
A. 
B. 
C. 
D. 
15.
A zone
of clearing on a lawn of bacteria caused by lysis of the cells by infecting bacteriophages
A. 
B. 
C. 
D. 
16.
A
collection of clones made from a set of randomly generated overlapping DNA
fragments representing the entire genome of an organism.
A. 
B. 
C. 
D. 
17.
The
acquisition by a cell of new genes by the uptake of naked DNA.
A. 
B. 
C. 
D. 
18.
The introduction of purified phage DNA molecules into a bacterial cell.
A. 
B. 
C. 
D. 
19.
A
cloning strategy whereby insertion of a new piece of DNA into a vector
inactivates a gene carried by the vector.
A. 
B. 
C. 
D. 
20.
A
high-capacity cloning vector based on the F plasmid of Escherichia coli.
A. 
B. 
C. 
D. 
21.
This plasmid
vectors replicate autonomously because of the presence of a segment of the
yeast 2 mm plasmid that serves as an origin of replication (2 mm ori)
A. 
B. 
C. 
D. 
22.
This plasmid
vectors do not replicate autonomously, but integrate into the genome at low frequencies
by homologous recombination.
A. 
B. 
C. 
D. 
23.
This plasmid
vectors are autonomously replicating vectors containing centromere sequences, CEN,
and autonomously replicating sequences, ARS.
A. 
B. 
C. 
D. 
24.
The
process of using a glass micropipette to insert substances at a microscopic or
borderline macroscopic level into a single living cell.
A. 
B. 
C. 
D. 
25.
A
means of introducing DNA into cells that involves bombardment with
high-velocity microprojectiles coated with DNA.
A. 
B. 
C. 
D.