Quiz Online 4 Chapter 5 Host Cells And Vectors
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- 1.Insertion of a fragment of DNA, containing a gene, into a cloning vector, and subsequent propagation of the recombinant DNA molecule in a host organism.
- A.
Gene cloning
- B.
Recombinant DNA
- C.
Cloning vector
- D.
Multiple cloning site
- 2.A DNA molecule created in the test tube by ligating pieces of DNA that are not normally joined together.
- A.
Gene cloning
- B.
Recombinant DNA
- C.
Cloning vector
- D.
Multiple cloning site
- 3.A cell that harbors foreign molecules, viruses, or microorganisms.
- A.
Gene cloning
- B.
E. coli
- C.
Cloning vector
- D.
Host
- 4.A vector that can replicate in the cells of more than one organism (e.g. in Escherichia coli and in yeast).
- A.
Shuttle vector
- B.
Insertion vector
- C.
Cloning vector
- D.
Replacement vector
- 5.A λ vector constructed by deleting a segment of non-essential DNA
- A.
Shuttle vector
- B.
Insertion vector
- C.
Cloning vector
- D.
Replacement vector
- 6.A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA.
- A.
Shuttle vector
- B.
Insertion vector
- C.
Cloning vector
- D.
Replacement vector
- 7.A λ vector designed so that insertion of new DNA is by replacement of part of the non-essential region of the λ DNA molecule.
- A.
Shuttle vector
- B.
Insertion vector
- C.
Cloning vector
- D.
Replacement vector
- 8.A gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector.
- A.
Origin of replication
- B.
Selectable marker
- C.
Copy number
- D.
Multiple cloning site
- 9.A short segment of DNA that contains several unique restriction endonuclease sites in cloning vector
- A.
Origin of replication
- B.
Selectable marker
- C.
Copy number
- D.
Multiple cloning site
- 10.A usually circular piece of DNA often found in bacteria and some other types of cell.
- A.
Plasmid
- B.
Phage
- C.
Cosmid
- D.
Phagemid
- 11.Cloning vector comprising a mixture of plasmid and phage DNA.
- A.
Plasmid
- B.
Phage
- C.
Cosmid
- D.
Phagemid
- 12.A virus that infects a bacterium.
- A.
Plasmid
- B.
Phage
- C.
Cosmid
- D.
Phagemid
- 13.A high-capacity cloning vector consisting of the λ cos site inserted into a plasmid.
- A.
Plasmid
- B.
Phage
- C.
Cosmid
- D.
Phagemid
- 14.A cell that has become transformed by the uptake of naked DNA.
- A.
Genomic library
- B.
Transformant
- C.
Host
- D.
Plaque
- 15.A zone of clearing on a lawn of bacteria caused by lysis of the cells by infecting bacteriophages
- A.
Genomic library
- B.
Transformant
- C.
Host
- D.
Plaque
- 16.A collection of clones made from a set of randomly generated overlapping DNA fragments representing the entire genome of an organism.
- A.
Genomic library
- B.
Transformant
- C.
Host
- D.
Plaque
- 17.The acquisition by a cell of new genes by the uptake of naked DNA.
- A.
Insertional inactivation
- B.
Transformation
- C.
Transgenic
- D.
Transfection
- 18.The introduction of purified phage DNA molecules into a bacterial cell.
- A.
Insertional inactivation
- B.
Transformation
- C.
Transgenic
- D.
Transfection
- 19.A cloning strategy whereby insertion of a new piece of DNA into a vector inactivates a gene carried by the vector.
- A.
Insertional inactivation
- B.
Transformation
- C.
Transduction
- D.
Transfection
- 20.A high-capacity cloning vector based on the F plasmid of Escherichia coli.
- A.
YAC
- B.
BAC
- C.
YEp
- D.
YIp
- 21.This plasmid vectors replicate autonomously because of the presence of a segment of the yeast 2 mm plasmid that serves as an origin of replication (2 mm ori)
- A.
YCp
- B.
BAC
- C.
YEp
- D.
YIp
- 22.This plasmid vectors do not replicate autonomously, but integrate into the genome at low frequencies by homologous recombination.
- A.
YCp
- B.
BAC
- C.
YEp
- D.
YIp
- 23.This plasmid vectors are autonomously replicating vectors containing centromere sequences, CEN, and autonomously replicating sequences, ARS.
- A.
YCp
- B.
BAC
- C.
YEp
- D.
YIp
- 24.The process of using a glass micropipette to insert substances at a microscopic or borderline macroscopic level into a single living cell.
- A.
Microinjection
- B.
Biolistic
- C.
Transformation
- D.
Electroporation
- 25.A means of introducing DNA into cells that involves bombardment with high-velocity microprojectiles coated with DNA.
- A.
Microinjection
- B.
Biolistic
- C.
Transformation
- D.
Electroporation
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