Transplantation And Hla Matching MCQ Assessment
This test consists of 24 multiple choice and short answer questions based on the short video. It should not take you more than 10-15 minutes. It does not require any revision. It is not timed. If you get stuck on a question, please leave it blank. Results are available immediately after the exam. To remain anonymous and for the purpose of statistical analysis, please enter your student number (including the two letters) as your first name, and your gender as your second name. THANK YOU FOR TAKING PART!
- 1.In a PRA test, if 40 out of 50 wells show cell death and take up the dye, then it can be estimated that:
- A.
10% of the population are suitable donors
- B.
20% of the population are unsuitable donors
- C.
80% of the population are unsuitable donors
- D.
10% of the population are unsuitable donors
- 2.Which statement regarding serum cross-matching and PRA is correct?
- A.
Both assays test for the presence of antibodies directed against antigens expressed on Red Blood Cells
- B.
The binding of anti-HLA antibodies to HLA antigens on donor cells leads to the activation of complement and the formation of the membrane attack complex
- C.
Formation of the membrane attack complex causes the donor macrophages to take up the dye
- D.
Antibodies in the serum are usually of the IgM subclass
- 3.Below are the HLA-A, -B and -DR types of two individuals Donor: A1, B8, B39, DR1, DR3 Recipient: A1, A24, B9, DR1, DR4 Please select all statements that apply:
- A.
The recipient is heterozygous for the HLA-DR allele
- B.
The donor is heterozygous for the HLA-A allele
- C.
The donor is homozygous for the HLA-A allele
- D.
The donor is homozygous for the HLA-DR allele
- E.
The recipient is heterozygous for the HLA-B allele
- 4.Which of these statements most applies to familial matching?
- A.
There is a 50% chance of a complete match between siblings
- B.
There is a 50% chance of a complete match between sibling and parent
- C.
There is a 25% chance of a complete match between siblings
- D.
HLA alleles are always 25% matched between parent and sibling
- 5.Which of these statements most applies to gel electrophoresis?
- A.
Gel electrophoresis relies on the positive charge of the DNA
- B.
Gel electrophoresis is a method for amplifying DNA
- C.
Gel electrophoresis describes the movement of ions across an applied electrical field
- D.
Gel electrophoresis separates molecules based on their electrical charge
- 6.Please identify the incorrect statements regarding agarose gel electrophoresis
- A.
A dye is used to track the migration of the DNA from the anode to the cathode
- B.
DNA is negatively charged
- C.
Smaller DNA fragments migrate faster towards the cathode
- D.
Ethidium bromide can be used to stain DNA
- E.
Smaller DNA fragments migrate faster towards the anode
- 7.For kidney transplants, it is essential to match:
- A.
HLA-A, HLA-B and HLA-C antigens
- B.
Only class I antigens
- C.
Only class II antigens
- D.
HLA-A, HLA-DR and HLA-DQ antigens
- E.
HLA-A, HLA-B and HLA-DR antigens
- 8.Please select the correct statement. MHC molecules are:
- A.
Glycoprotein complexes encoded for by genes found on chromosome 6
- B.
Expressed only on human cells
- C.
Are encoded for by 4 gene clusters on chromosome 3
- D.
Show little variation between individuals
- 9.Which of the following statements is incorrect?
- A.
MHC class I molecules are encoded for by HLA-A,HLA-B and HLA-C genes
- B.
MCH class II molecules are encoded for by HLA-A-, HLA-B and HLA-DR genes
- C.
MHC class III molecules encode various components of complement
- D.
MHC class II molecules are encoded for by HLA-DR, HLA-DP and HLA-DQ genes
- 10.Which of these statements is correct?
- A.
SSP-PCR is a molecular method used to detect antibodies
- B.
Serum cross matching detects pre-existing donor specific antibodies
- C.
SSP-PCR is a serological method used to assign a HLA tissue-type
- D.
SSP-PCR requires the used of specific DNA primers
- 11.Which of these statements regarding SSP-PCR is incorrect?
- A.
A single cycle consists of three stages
- B.
The PCR process is used as an amplification step followed by a post-amplification step to detect specific alleles
- C.
In the absence of an amplification product it can be assumed that the individual does not express a specific allele
- D.
SSP-PCR allows for the amplification of specific HLA alleles
- 12.The human leukocyte antigens are otherwise known as ________ molecules.
- 13.In total, there are ________ different classes of HLA molecule.
- 14.HLA antigens are encoded for by genes found on chromosome ________
- 15.Individuals that are antigenically similar are said to be ________ .
- 16.HLA-A, -B and -C antigens are encoded for by class _______ genes.
- 17.HLA-DR, -DP and -DQ antigens are encoded for by class ________ genes.
- 18.HLA genes are very close together. As such they are inherited en bloc. We each inherit two _________, one from each parent.
- 19.HLA alleles are expressed in a ________ fashion.
- 20.HLA typing can be done using ________ and molecular methods.
- 21.Pre-transplant tests include ABO blood typing, HLA typing, ________ cross-matching and the PRA.
- 22.The PRA is an abbreviation for the ________ ________ ________ test . It estimates the percentage of the population that are likely to be unsuitable donors.
- 23.How many mismatches are there for the HLA-A locus?
- 24.How many mismatches are there for the HLA-B locus?
- 25.How many mismatches are there for the HLA-DR locus?
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