Microbiology Lab Exam

30 Questions | Total Attempts: 641

SettingsSettingsSettings
Please wait...
Microbiology Lab Quizzes & Trivia

Study up on you micro, use deduction to determine what bacteria is in your culture. Plus test your memory on the different media


Questions and Answers
  • 1. 
    What are the properties of MSA? 
    • A. 

      Salt and Mannitol

    • B. 

      Not selective and egg yolk

    • C. 

      Bile salts and Lactose

  • 2. 
    What are the properties of MAC agar?
    • A. 

      Salt + mannitol

    • B. 

      Bile salts + lactose

    • C. 

      Bile salts + egg yolk

  • 3. 
    Which of the following are lactose positive, that is turns red or pink on MAC agar?
    • A. 

      Proteus

    • B. 

      Enterobacter

    • C. 

      Shigella

  • 4. 
    Which of the following do not ferment lactose, that is turns light brown on MAC agar?
    • A. 

      E. coli

    • B. 

      Klebsiella

    • C. 

      Salmonella

  • 5. 
    Which of the following are lactose negative, that is it turns brown on MAC agar?
    • A. 

      Shigella

    • B. 

      Enterobacter

    • C. 

      Klebsiella

  • 6. 
    Which of the following is a lactose positive bacteria? 
    • A. 

      Klebsiella

    • B. 

      Salmonella

    • C. 

      Proteus

  • 7. 
    Does E.coli turn red/ pink on MAC agar, giving a positive reaction?
    • A. 

      True

    • B. 

      False

  • 8. 
    Egg yolk contains ___________ which can be broken down into fatty acids by the enzyme ____________
  • 9. 
    The control LIA tube tests for?
    • A. 

      Growth

    • B. 

      Glucose fermentation

    • C. 

      Decarboxylation of lysine

  • 10. 
    In O/F tubes, if there is growth in both tubes the bacteria is?
    • A. 

      Anaerobice

    • B. 

      Facultative anaerobe

    • C. 

      Aerobic

  • 11. 
    In a O/F tube, the tube goes what colour if it can ferment glucose?
    • A. 

      Yellow

    • B. 

      Pink

    • C. 

      Purple

  • 12. 
    What colour is the slope of a TSIA tube if the bacteria can utilise lactose and/or sucrose?
    • A. 

      Yellow

    • B. 

      Pink

    • C. 

      Purple

  • 13. 
    What colour is the slope of an TSIA tube if the bacteria cannot ferment lactose and/or sucrose?
    • A. 

      Yellow

    • B. 

      Pink

    • C. 

      Purple

  • 14. 
    What causes the black precipitate in a TSIA tube?
    • A. 

      Sulphide + iron salt = ferrous sulphide

    • B. 

      Tellurite reduction

  • 15. 
    Which media is selective and differential for Corynebacterium?
    • A. 

      HBA

    • B. 

      TBA

    • C. 

      MAC

  • 16. 
    If the VP test comes back positive, what is the result of MR?
    • A. 

      Positive

    • B. 

      Negative

  • 17. 
    Which combination is true for E.coli?
    • A. 

      Lac +, VP -, CIT +

    • B. 

      Lac -, ONPG +, Urease +

    • C. 

      Lac +, MR +, indole +

  • 18. 
    Which are Urease positive?
    • A. 

      E. coli

    • B. 

      Protease

    • C. 

      Salmonella

    • D. 

      Klebsiella

  • 19. 
    Which of the following is VP positive?
    • A. 

      E. coli

    • B. 

      Enterobacter

    • C. 

      Proteus

    • D. 

      Salmonella

    • E. 

      Shigella

    • F. 

      Klebsiella

  • 20. 
    Shigella and Klebsiella are both motile
    • A. 

      True

    • B. 

      False

  • 21. 
    Proteus and Salmonella are both ONPG negative
    • A. 

      True

    • B. 

      False

  • 22. 
    Shigella ferment glucose to acid and gas
    • A. 

      True

    • B. 

      False

  • 23. 
    Which of the following decarboxylate lysine in LIA tubes?
    • A. 

      E. coli

    • B. 

      Enterobacter

    • C. 

      Proteus

    • D. 

      Salmonella

    • E. 

      Shigella

    • F. 

      Klebsiella

  • 24. 
    Which Enteric is the only Indole positive bacteria?
    • A. 

      Enterobacter

    • B. 

      E. coli

    • C. 

      Salmonella

  • 25. 
    Which of the following produse H2S?
    • A. 

      E. coli

    • B. 

      Enterobacter

    • C. 

      Proteus

    • D. 

      Salmonella

    • E. 

      Shigella

    • F. 

      Klebsiella

Back to Top Back to top