Microbiology Lab Exam #1

34 Questions | By Caitlin.mumaw | Last updated: Jan 18, 2013 | Total Attempts: 7192

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Quiz to study for McNeely's Lab Exam #1:Includes: oil immersionaseptic techniquestreaking for isolationgram stainingacid-fast stainingspecial staining

Questions and Answers

1.

What two parts of the microscope are used to carry or support the microscope?
2.

What are the three optical parts of a microscope?
3.

Red colored objective is called what?
- A.
  
  Scanning
- B.
  
  Low-dry
- C.
  
  Oil immersion
4.

What total magnification does the scanning objective have?
- A.
  
  4
- B.
  
  40
- C.
  
  10
5.

What is the real name for the blue-rimmed objective?
- A.
  
  High-dry
- B.
  
  Low-dry
- C.
  
  Scanning
6.

What is the real name for the yellow-rimmed objective?
- A.
  
  Low-dry
- B.
  
  High-dry
- C.
  
  Oil immersion
7.

What is the real name of the white-rimmed objective?
- A.
  
  Scanning
- B.
  
  Low-dry
- C.
  
  Oil immersion
8.

What is the total magnification for low-dry?
- A.
  
  40
- B.
  
  1000
- C.
  
  100
9.

What is the total magnification for high-dry?
- A.
  
  400
- B.
  
  40
- C.
  
  1000
10.

What is the total magnification for oil immersion?
- A.
  
  10
- B.
  
  400
- C.
  
  1000
11.

What are the functions of the abbe condenser?
- A.
  
  Shuts off the light
- B.
  
  Collects light
- C.
  
  Condenses light
- D.
  
  Alternate light source
- E.
  
  Only light source
12.

What is the function of the iris diaphragm?
- A.
  
  Turns light on, controls light
- B.
  
  Adjustment for light coming through the abbe condenser; want it to ideally be in the middle; when looking through lens, light shouldn't be blinding.
13.

Four microscopic morphologies of bacteria?
14.

Parfocal lenses allow for us to be able to go between objectives with little adjustment of ___.
- A.
  
  Iris diaphragm
- B.
  
  Abbe condenser
- C.
  
  Fine focus
15.

What is the purpose of oil immersion?
- A.
  
  To use oil to replace air and to prevent refraction at a glass-air interface.
- B.
  
  To use oil to replace air to prevent absorption of bacteria
16.

How do you get total magnification (equation)
17.

What is the appropriate order of clean up?1. clean stage with used alcohol swab2. clean ocular lenses with alcohol swab and take lense paper to clean/wipe of oculars, polish3. clean yellow, blue, white objectives
- A.
  
  3, 2, 1
- B.
  
  1, 2, 3,
- C.
  
  2, 3, 1
18.

Why is sheep's blood used as media?
- A.
  
  Non-fastidious bacteria
- B.
  
  Fastidious bacteria
- C.
  
  Requires extra nutirents
19.

What are five characteristics of bacterial colonial morphologies?
- A.
  
  Differences in size
- B.
  
  Shape
- C.
  
  Color or pigment
- D.
  
  Weight
- E.
  
  Toxicity
- F.
  
  Texture
- G.
  
  Density
20.

Why should a petri dish not be left open for an extended period of time?
21.

What does streaking for isolation mean?
22.

Purpose of inverting plates during incubation?
- A.
  
  To not allow bacteria to unnecessarily clump or to let it dissolve into agar
- B.
  
  To prevent buildup of condensation that would ruin the culture
23.

What is normal flora?
- A.
  
  Air contamination
- B.
  
  Buildup of condensation
- C.
  
  Grows on different mucosal surfaces of the body
24.

Four places normal flora exists
- A.
  
  GI tract
- B.
  
  Vagina
- C.
  
  Armpit
- D.
  
  Throat, mouth
- E.
  
  Nose
- F.
  
  Sinuses
25.

What color is serratia at room temperature?