Microbiology Lab Exam #1
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Quiz to study for McNeely's Lab Exam #1:Includes: oil immersionaseptic techniquestreaking for isolationgram stainingacid-fast stainingspecial staining
- 1.What two parts of the microscope are used to carry or support the microscope?
- 2.What are the three optical parts of a microscope?
- 3.Red colored objective is called what?
- A.
Scanning
- B.
Low-dry
- C.
Oil immersion
- 4.What total magnification does the scanning objective have?
- A.
4
- B.
40
- C.
10
- 5.What is the real name for the blue-rimmed objective?
- A.
High-dry
- B.
Low-dry
- C.
Scanning
- 6.What is the real name for the yellow-rimmed objective?
- A.
Low-dry
- B.
High-dry
- C.
Oil immersion
- 7.What is the real name of the white-rimmed objective?
- A.
Scanning
- B.
Low-dry
- C.
Oil immersion
- 8.What is the total magnification for low-dry?
- A.
40
- B.
1000
- C.
100
- 9.What is the total magnification for high-dry?
- A.
400
- B.
40
- C.
1000
- 10.What is the total magnification for oil immersion?
- A.
10
- B.
400
- C.
1000
- 11.What are the functions of the abbe condenser?
- A.
Shuts off the light
- B.
Collects light
- C.
Condenses light
- D.
Alternate light source
- E.
Only light source
- 12.
- A.
Turns light on, controls light
- B.
Adjustment for light coming through the abbe condenser; want it to ideally be in the middle; when looking through lens, light shouldn't be blinding.
- 13.Four microscopic morphologies of bacteria?
- 14.Parfocal lenses allow for us to be able to go between objectives with little adjustment of ___.
- A.
Iris diaphragm
- B.
Abbe condenser
- C.
Fine focus
- 15.What is the purpose of oil immersion?
- A.
To use oil to replace air and to prevent refraction at a glass-air interface.
- B.
To use oil to replace air to prevent absorption of bacteria
- 16.How do you get total magnification (equation)
- 17.What is the appropriate order of clean up?1. clean stage with used alcohol swab2. clean ocular lenses with alcohol swab and take lense paper to clean/wipe of oculars, polish3. clean yellow, blue, white objectives
- A.
3, 2, 1
- B.
1, 2, 3,
- C.
2, 3, 1
- 18.Why is sheep's blood used as media?
- A.
Non-fastidious bacteria
- B.
Fastidious bacteria
- C.
Requires extra nutirents
- 19.What are five characteristics of bacterial colonial morphologies?
- A.
Differences in size
- B.
Shape
- C.
Color or pigment
- D.
Weight
- E.
Toxicity
- F.
Texture
- G.
Density
- 20.Why should a petri dish not be left open for an extended period of time?
- 21.What does streaking for isolation mean?
- 22.Purpose of inverting plates during incubation?
- A.
To not allow bacteria to unnecessarily clump or to let it dissolve into agar
- B.
To prevent buildup of condensation that would ruin the culture
- 23.What is normal flora?
- A.
Air contamination
- B.
Buildup of condensation
- C.
Grows on different mucosal surfaces of the body
- 24.Four places normal flora exists
- A.
GI tract
- B.
Vagina
- C.
Armpit
- D.
Throat, mouth
- E.
Nose
- F.
Sinuses
- 25.What color is serratia at room temperature?
- 26.What two things should you always do to enhance isolation of bacteria?
- A.
Flame and cool needle after primary inoculum
- B.
Firm touch, don't scoop
- C.
Scoop up bacteria
- 27.What is media?
- A.
Where bacteria is taken from originally
- B.
Broth or agar based
- C.
"base" for bacteria to be added to
- 28.What and where is agar from?
- A.
Solid form of media
- B.
Liquid form of media
- C.
Comes from seaweed
- D.
Comes from pebbles
- 29.What are ways to tell if media is sterile?
- A.
Run controls
- B.
Inoculate with bacteria
- C.
No floaters in agar
- D.
No growth in broth
- 30.Why are culture tubes held at an angle?
- 31.What are two possible errors which could have occurred if you find your subcultures had no growth after incubation for 48 hours?
- A.
Not a sterile plate
- B.
Forgetting to inoculate
- C.
Could have killed bacteria with heat from un-cooled needle
- 32.What are things to do while using a bunsen burner?
- A.
Have hair down and near bunsen burner
- B.
Never lean over burner with flame on
- C.
Light match before turning gas flow on
- 33.What does a positive acid-fast stain look like?
- 34.What group of organisms are considered acid-fast?
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