MCMH MICROBIOLOGY LAB COMPETENCY 2010

2.

In a few sentences describe what you would do if you received an order from the floor for occult blood random on a stool.

3.

The D - test quality control needs to be set up:

Monthly
Weekly
On each day of patient use
Daily

Correct Answer

A. On each day of patient use

Explanation

The D - test quality control needs to be set up on each day of patient use to ensure the accuracy and reliability of the test results. Setting up the quality control on a daily basis allows for regular monitoring and detection of any potential issues or errors that may arise during the testing process. This ensures that the test results are consistently accurate and reliable, providing healthcare professionals with reliable information for patient diagnosis and treatment.

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0

4.

Which of the following types of gram stains needs to be performed on all shifts?

CSF
Positive Blood Culture
STAT! Body Fluid
STAT! gram stain on OR/Surgical specimen

Explanation

The correct answer is STAT! gram stain on OR/Surgical specimen. This type of gram stain needs to be performed on all shifts because it is time-sensitive and the results are needed urgently in order to guide immediate patient care decisions during surgery or other invasive procedures.

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5.

What drug was developed to "beat out" the resistance to penicillins?

Explanation
Oxacillin was developed to overcome the resistance to penicillins. It is a type of penicillinase-resistant penicillin, meaning it is not easily broken down by the enzyme penicillinase that some bacteria produce to resist the effects of penicillins. By modifying the structure of the penicillin molecule, oxacillin was able to evade the action of penicillinase and effectively treat infections caused by penicillin-resistant bacteria.

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6.

A strep culture has been ordered on a throat swab. What pathogenic organisms should be reported?

Group A strep that is PYR positive and Latex Grp A positive
Group A strep that is PYR negative but latex Grp A positive
Group B strep
Group F strep

Correct Answer

A. Group A strep that is PYR positive and Latex Grp A positive

Explanation

The correct answer is "Group A strep that is PYR positive and Latex Grp A positive." This is because a strep culture is specifically ordered to identify Group A streptococcus, which is known to cause various infections such as strep throat. The presence of PYR positivity and positive latex test for Group A strep confirms the identification of this pathogenic organism. Group B strep and Group F strep are not relevant in this context as they are not the target organisms for the strep culture.

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7.

The gram positive organsms shown here are more than likely what?

Diptheroids
E.coli
Staphlyococci
Neisseria species

Explanation

The gram positive organisms shown here are more than likely Staphylococci. This can be inferred from their characteristic appearance under Gram staining, where they appear as clusters of spherical cells. Staphylococci are commonly found on the skin and mucous membranes of humans and animals, and they are known to cause a variety of infections, including skin infections, pneumonia, and bloodstream infections.

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2

8.

The organism Staphlyococcus saprophyticus is

Coagulase negative
Novobiocin resistant
Beta-hemolytic
A pathogen of concern for women of childbearing age

Correct Answer(s)

A. Coagulase negative
A. Novobiocin resistant
A. A pathogen of concern for women of childbearing age

Explanation

Staphylococcus saprophyticus is a coagulase-negative bacterium, meaning it does not produce the enzyme coagulase. It is also resistant to novobiocin, an antibiotic commonly used to treat staphylococcal infections. Additionally, it is considered a pathogen of concern for women of childbearing age, as it is a common cause of urinary tract infections in this population.

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1

9.

What 2 comments need to be added to a urine culture report that has >100,000 CFU/mL Enterococci species that is positive on the Vancomycin Screen and has been confirmed by the Microscan as Enterococcus faecium?

Correct Answer(s)
Vancomycin Resistant Enterococci isolated and ENTEROCOCCUS ARE SCREENED FOR VANCOMYCIN RESISTANCE FOR EPIDEMIOLOGICAL PURPOSES. AMPICILLIN IS THE DRUG OF CHOICE FOR TREATING ENTEROCOCCAL INFECTIONS RESTRICTED TO THE URINARY TRACT.
MVRE

Explanation
The two comments that need to be added to the urine culture report are: "Vancomycin Resistant Enterococci isolated and ENTEROCOCCUS ARE SCREENED FOR VANCOMYCIN RESISTANCE FOR EPIDEMIOLOGICAL PURPOSES" and "AMPICILLIN IS THE DRUG OF CHOICE FOR TREATING ENTEROCOCCAL INFECTIONS RESTRICTED TO THE URINARY TRACT." The first comment indicates that the Enterococci species isolated from the urine culture are resistant to vancomycin, which is important information for treatment decisions and infection control measures. The second comment provides guidance on the appropriate drug choice for treating Enterococcal infections that are limited to the urinary tract.

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10.

MRSA's are ________________________________ resistant.

Correct Answer(s)
Oxacillin

Explanation
MRSA's are resistant to Oxacillin.

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11.

This is a gram stain typical of what organism classifcation?

Pseuodmonas species
Staphylococci
Streptococci
Diptheroids

Correct Answer

A. Diptheroids

Explanation

Diptheroids are a type of bacteria that are commonly found on the skin and mucous membranes of humans. They are gram-positive bacteria, meaning they retain the crystal violet stain during the gram staining process. This distinguishes them from gram-negative bacteria like Pseudomonas species. Staphylococci and Streptococci are also gram-positive bacteria, but they have distinct characteristics that differentiate them from diptheroids. Therefore, based on the gram stain, the given bacteria are most likely diptheroids.

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12.

Specimens that are collected in viral transport can be processed for routine bacterial culture and sensitivity testing?

TRUE
FALSE

Correct Answer

A. FALSE

Explanation

Specimens that are collected in viral transport media are specifically designed to preserve and transport viruses, not bacteria. Therefore, these specimens cannot be used for routine bacterial culture and sensitivity testing.

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13.

Specimens for the microbiology lab should be processed

As soon as possible because the length of incubation time does have a bearing on microbiology culture results
As a batch because it saves time and the length of incubation time has no bearing on microbiology culture results
Have the lowest priority when working on the evening/nite shifts in the laboratory
Only by the microbiology department technologists

Correct Answer

A. As soon as possible because the length of incubation time does have a bearing on microbiology culture results

Explanation

Specimens for the microbiology lab should be processed as soon as possible because the length of incubation time does have a bearing on microbiology culture results. This means that the longer the specimens are left without being processed, the more time bacteria or other microorganisms have to grow and potentially affect the results of the culture. Processing the specimens quickly helps ensure accurate and reliable results.

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14.

A specimen collected from the Emergency Department for RSV, INFLUENZA A/B, AND RESPIRATORY VIRAL PROFILE needs to be sent down in three separate viral transport media containers.

FALSE
TRUE

Correct Answer

A. FALSE

Explanation

ONLY ONE VIRAL TRANSPORT SPECIMEN CONTAINER IS NECESSARY FOR PROCESSING ALL THREE TESTS. KEEPING IN MIND THAT THE SPECIMEN NEEDS TO BE SAVED IN THE MICRO LAB SO THAT IT CAN BE SENT OUT TO IU/CLARIAN FOR THE RESPIRATORY VIRAL PROFILE BY THE MICROBIOLOGY DEPARTMENT THE FOLLOWING SHIFT.

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15.

Microbiology is the BEST section of the laboratory to work in :-)

True
False

Correct Answer

A. True

Explanation

Microbiology is considered the best section of the laboratory to work in because it involves studying microorganisms, which are fascinating and diverse. Microbiologists have the opportunity to make significant contributions to various fields such as medicine, agriculture, and environmental science. They play a crucial role in identifying and combating infectious diseases, developing new drugs and vaccines, and understanding the complex interactions between microbes and their environments. Additionally, working in microbiology allows for constant learning and discovery, as new microorganisms and their behaviors are constantly being uncovered. Overall, microbiology offers a rewarding and intellectually stimulating career path.

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Mcmh Microbiology Lab Competency 2010

Can you tell me "how" MRSA's are penicillin resistant and what the drug of choice is for MRSA infections?

Quiz Preview

In a few sentences describe what you would do if you received an order from the floor for occult blood random on a stool.

The D - test quality control needs to be set up:

Which of the following types of gram stains needs to be performed on all shifts?

What drug was developed to "beat out" the resistance to penicillins?

A strep culture has been ordered on a throat swab. What pathogenic organisms should be reported?

The gram positive organsms shown here are more than likely what?

The organism Staphlyococcus saprophyticus is

What 2 comments need to be added to a urine culture report that has >100,000 CFU/mL Enterococci species that is positive on the Vancomycin Screen and has been confirmed by the Microscan as Enterococcus faecium?

MRSA's are ________________________________ resistant.

This is a gram stain typical of what organism classifcation?

Specimens that are collected in viral transport can be processed for routine bacterial culture and sensitivity testing?

Specimens for the microbiology lab should be processed

A specimen collected from the Emergency Department for RSV, INFLUENZA A/B, AND RESPIRATORY VIRAL PROFILE needs to be sent down in three separate viral transport media containers.

Microbiology is the BEST section of the laboratory to work in :-)