Quiz On Gene Therapy (Ch-20)
.
- 1.Which of the following produces multiple identical copies of a gene for basic research or for large-scale production of a gene product?
- A.
Restriction enzymes
- B.
Gene cloning
- C.
DNA ligase
- D.
Gel electrophoresis
- E.
Reverse transcriptase
- 2.Which of the following cuts DNA molecules at specific locations?
- A.
Restriction enzymes
- B.
Gene cloning
- C.
DNA ligase
- D.
Gel electrophoresis
- E.
Reverse transcriptase
- 3.Which of the following separates molecules by movement due to size and electrical charge?
- A.
Restriction enzymes
- B.
Gene cloning
- C.
DNA ligase
- D.
Gel electrophoresis
- E.
Reverse transcriptase
- 4.Which of the following is used to make complementary DNA (cDNA) from RNA?
- A.
Restriction enzymes
- B.
Gene cloning
- C.
DNA ligase
- D.
Gel electrophoresis
- E.
Reverse transcriptase
- 5.Which of the following seals the sticky ends of restriction fragments to make recombinant DNA?
- A.
Restriction enzymes
- B.
Gene cloning
- C.
DNA ligase
- D.
Gel electrophoresis
- E.
Reverse transcriptase
- 6.RFLP analysis can be used to distinguish between alleles based on differences in which of the following?
- A.
The proteins expressed from the alleles
- B.
The amount of DNA amplified from the alleles during PCR
- C.
The ability of the alleles to be replicated in bacterial cells
- D.
Restriction enzyme recognition sites between the alleles
- E.
The ability of nucleic acid probes to hybridize to the alleles
- 7.Which was developed by a British researcher and causes DNA sequences to be transferred to a membrane and identified with a probe?
- A.
Southern blotting
- B.
Northern blotting
- C.
Western blotting
- D.
Eastern blotting
- E.
RT-PCR
- 8.Which describes the transfer of polypeptide sequences to a membrane to analyze gene expression?
- A.
Southern blotting
- B.
Northern blotting
- C.
Western blotting
- D.
Eastern blotting
- E.
RT-PCR
- 9.Which uses reverse transcriptase to make cDNA followed by amplification?
- A.
Southern blotting
- B.
Northern blotting
- C.
Western blotting
- D.
Eastern blotting
- E.
RT-PCR
- 10.Which of the following is one of the technical reasons why gene therapy is problematic?
- A.
Most cells with an engineered gene do not produce gene product.
- B.
Most cells with engineered genes overwhelm other cells in a tissue.
- C.
Cells with transferred genes are unlikely to replicate.
- D.
Transferred genes may not have appropriately controlled activity.
- E.
MRNA from transferred genes cannot be translated.
- 11.One possible use of transgenic plants is in the production of human proteins, such as vaccines. Which of the following is a possible hindrance that must be overcome?
- A.
Prevention of transmission of plant allergens to the vaccine recipients
- B.
Prevention of vaccine-containing plants being consumed by insects
- C.
Use of plant cells to translate non-plant derived mRNA
- D.
Inability of the human digestive system to accept plant-derived protein
- E.
The need to cook all such plants before consuming them
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