Quiz On Gene Therapy (Ch-20)

11 Questions | Total Attempts: 361

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Quiz On Gene Therapy (Ch-20)

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Questions and Answers
  • 1. 
    Which of the following produces multiple identical copies of a gene for basic research or for large-scale production of a gene product?
    • A. 

      Restriction enzymes

    • B. 

      Gene cloning

    • C. 

      DNA ligase

    • D. 

      Gel electrophoresis

    • E. 

      Reverse transcriptase

  • 2. 
    Which of the following cuts DNA molecules at specific locations?
    • A. 

      Restriction enzymes

    • B. 

      Gene cloning

    • C. 

      DNA ligase

    • D. 

      Gel electrophoresis

    • E. 

      Reverse transcriptase

  • 3. 
    Which of the following separates molecules by movement due to size and electrical charge?
    • A. 

      Restriction enzymes

    • B. 

      Gene cloning

    • C. 

      DNA ligase

    • D. 

      Gel electrophoresis

    • E. 

      Reverse transcriptase

  • 4. 
    Which of the following is used to make complementary DNA (cDNA) from RNA?
    • A. 

      Restriction enzymes

    • B. 

      Gene cloning

    • C. 

      DNA ligase

    • D. 

      Gel electrophoresis

    • E. 

      Reverse transcriptase

  • 5. 
    Which of the following seals the sticky ends of restriction fragments to make recombinant DNA?
    • A. 

      Restriction enzymes

    • B. 

      Gene cloning

    • C. 

      DNA ligase

    • D. 

      Gel electrophoresis

    • E. 

      Reverse transcriptase

  • 6. 
    RFLP analysis can be used to distinguish between alleles based on differences in which of the following?
    • A. 

      The proteins expressed from the alleles

    • B. 

      The amount of DNA amplified from the alleles during PCR

    • C. 

      The ability of the alleles to be replicated in bacterial cells

    • D. 

      Restriction enzyme recognition sites between the alleles

    • E. 

      The ability of nucleic acid probes to hybridize to the alleles

  • 7. 
    Which was developed by a British researcher and causes DNA sequences to be transferred to a membrane and identified with a probe?
    • A. 

      Southern blotting

    • B. 

      Northern blotting

    • C. 

      Western blotting

    • D. 

      Eastern blotting

    • E. 

      RT-PCR

  • 8. 
    Which describes the transfer of polypeptide sequences to a membrane to analyze gene expression?
    • A. 

      Southern blotting

    • B. 

      Northern blotting

    • C. 

      Western blotting

    • D. 

      Eastern blotting

    • E. 

      RT-PCR

  • 9. 
    Which uses reverse transcriptase to make cDNA followed by amplification?
    • A. 

      Southern blotting

    • B. 

      Northern blotting

    • C. 

      Western blotting

    • D. 

      Eastern blotting

    • E. 

      RT-PCR

  • 10. 
    Which of the following is one of the technical reasons why gene therapy is problematic?
    • A. 

      Most cells with an engineered gene do not produce gene product.

    • B. 

      Most cells with engineered genes overwhelm other cells in a tissue.

    • C. 

      Cells with transferred genes are unlikely to replicate.

    • D. 

      Transferred genes may not have appropriately controlled activity.

    • E. 

      MRNA from transferred genes cannot be translated.

  • 11. 
    One possible use of transgenic plants is in the production of human proteins, such as vaccines. Which of the following is a possible hindrance that must be overcome?
    • A. 

      Prevention of transmission of plant allergens to the vaccine recipients

    • B. 

      Prevention of vaccine-containing plants being consumed by insects

    • C. 

      Use of plant cells to translate non-plant derived mRNA

    • D. 

      Inability of the human digestive system to accept plant-derived protein

    • E. 

      The need to cook all such plants before consuming them

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