Bimm 116 Final

1. Which of the following rhythms would likely be measured by use of luciferase expressed in Drosophila?

A) locomotor activity

C) degradation of the CRY protein

B) production of mRNA from the per gene

D) phosphorylation of PER

Luciferase expressed in Drosophila is commonly used as a reporter gene to measure the production of mRNA, making option B the correct answer. The other options do not directly involve the use of luciferase and are not commonly utilized for this purpose.

Explanation

Luciferase expressed in Drosophila is commonly used as a reporter gene to measure the production of mRNA, making option B the correct answer. The other options do not directly involve the use of luciferase and are not commonly utilized for this purpose.

2. What conditions could NOT produce entrainment to a 26 h day?

DL1:25

D) LL

LD14:12

LD13:13

In the context of circadian rhythms, LL (constant light) does not allow entrainment to a 26 h day as light-dark cycles are necessary for proper entrainment.

Explanation

In the context of circadian rhythms, LL (constant light) does not allow entrainment to a 26 h day as light-dark cycles are necessary for proper entrainment.

3. What does double plotting refer to?

D) showing all activity twice on an actogram

C) accelerating the rhythm's rate two-fold

A) validating temperature values by measuring them twice

B) using dawn and dusk light pulses to simulate a natural day

4. If a wildtype (normal) hamster has its SCN lesioned and is given a transplant of SCN tissue from a tau/tau-mutant hamster, what is likely to be observed?

D) an activity rhythm with a period near 20 h

An activity rhythm with a period near 24 h

Restoration of hormonal rhythms, but not activity rhythms

Restoration of all rhythms as they were initially

When a wildtype hamster receives SCN tissue from a tau/tau-mutant hamster, the tau/tau mutation is likely to cause the activity rhythm to shift, resulting in an activity rhythm with a period near 20 hours. This is due to the effects of the tau mutation on the circadian clock system.

Explanation

When a wildtype hamster receives SCN tissue from a tau/tau-mutant hamster, the tau/tau mutation is likely to cause the activity rhythm to shift, resulting in an activity rhythm with a period near 20 hours. This is due to the effects of the tau mutation on the circadian clock system.