Molecular Biology Exam 2

83 Questions  I  By Ekanye on March 31, 2010
Cover chapter 6-13 for exam 2. 

  

Question Excerpt

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1.  Which of the following is not present in the core RNA polymerase? 
A.
B.
C.
D.
2.  An experiment was designed to obtained nonspecific transcription from both strands of a DNA molecule. Which of the following strategies would be most effective in achieving this? 
A.
B.
C.
D.
E.
3.  Which of the following techniques would be most useful in testing the ability of the core RNA polymerase to bind to promoter? 
A.
B.
C.
D.
E.
4.  Which of the following statements is false regarding the sigma-factor? 
A.
B.
C.
D.
E.
5.  Which of the following is true about the holoenzyme of RNA pol in an open complex? 
A.
B.
C.
D.
E.
6.  Dimethyl sulfate is used to 
A.
B.
C.
D.
E.
7.  Which of the following would most likely occur if an in vitro transcription assay is preformed by using a cell extract containing an (alpha) a subunit without the C-terminal domain? 
A.
B.
C.
D.
E.
8.  Place the following in the order that they occur during transcription initiation: 1- formation of open complex2- formation of closed complex3- promoter clearance4- synthesis of about 10 nucleotides 
A.
B.
C.
D.
E.
9.  Which of the following techniques is suitable for the study of DNA:RNA hybrid formation during transcription? 
A.
B.
C.
D.
E.
10.  Which of the following are characteristic of intrinsic terminator elements? 
A.
B.
C.
D.
E.
11.  Which of the following is incorrect regarding rho dependent terminators? 
A.
B.
C.
D.
E.
12.  Which of the following does not have a DNA-binding domain? 
A.
B.
C.
D.
E.
13.  Which of the following bond is lactose links between glucose and galactose? 
A.
B.
C.
D.
E.
14.  If an operon is under negative control it means that the operon is never operational
A.
B.
15.  In the absence of glucose there will be a rapid accumulation of lactose in the following mutant: I- Oc Z+ Y-I- O+ Z+ Y-
A.
B.
16.  The following is an example of a cis-dominant mutation: I-d O+ Z+ Y+ A-
A.
B.
17.  Which of the following statements is true about a lac operon with the following genotype? I- Oc Z+ Y-I- O+ Z- Y-
A.
B.
C.
D.
E.
18.  Which of the following plasmids could be used to restore inducible regulation of (Beta)-galatosidase in this mutatnt: I+ Oc Z- Y+ A+? 
A.
B.
C.
D.
E.
19.  Which of the following organisms would produce functional lac products in the presence of IPTG? 
A.
B.
C.
D.
E.
20.  Which of the followign statements is true about a lac operon with this genotype? I-d O+ Z+ Y- A- 
A.
B.
C.
D.
E.
21.  Which of the following techniques is most useful in determining if RNA polymerase has initiated transcription form the lac DNA template? 
A.
B.
C.
D.
E.
22.  In an in vitro transcription assay to detect the level of transcription from a lac operon, one of the RNA precursors was tagged with a fluorescent label on the y-phosphate. Which of the following is a likely outcome if transcription occurred? 
A.
B.
C.
D.
E.
23.  Which of the following is mostly likely to occur if the level of glucose is low in a bacterial cell? 
A.
B.
C.
D.
E.
24.  Which of the following is true about the action of CAP at the lac promoter? 
A.
B.
C.
D.
E.
25.  Which of the following is true about the regulation of the trp operon? 
A.
B.
C.
D.
E.
26.  Which of the following is absent in an operon? 
A.
B.
C.
D.
E.
27.  Recent experimental evidence has shown that only one operator sequence is needed for maximal expression 
A.
B.
28.  One hypothesis explaining the mechanism of repression of the lac operon states that the repressor binds the operator and blocks access by the polymerase to the adjacent promoter
A.
B.
29.  CAMP level is increased by the presence of high level of glucose 
A.
B.
30.  Arabibinose can derepress the ara operon by causing AraC to loosen its attachement to araO2 and to bind to araI2 instead. 
A.
B.
31.  Attenuation causes the length of the RNA molecules to be significantly extended. 
A.
B.
32.  The following conditions lead to a change in gene expression patterns in bacterial cells 
A.
B.
C.
D.
E.
33.  Which of the following sigma specificity factor is involved in middle gene transcription during SPO1 phage infection of bacteria?
A.
B.
C.
D.
E.
34.  Which of the following is true about the early phase of T7 infection of bacteria?
A.
B.
C.
D.
E.
35.  Differences in transcription of T7 and SPO1 genes are 
A.
B.
C.
D.
E.
36.  Which of the following conditions does not lead to a change in gene expression patterns in bacteria cells? 
A.
B.
C.
D.
E.
37.  Predict the outcome of the removal of the N form the DNA sequence of the (lamda) phage:
A.
B.
C.
D.
E.
38.  Which of the following is a possible mechanism for the action of NusA in termination? 
A.
B.
C.
D.
E.
39.  Which of the following is involved in maintaining lysogeny in (lamda) phage? 
A.
B.
C.
D.
E.
40.  Which of the following operators is a target of CI gene production? 
A.
B.
C.
D.
E.
41.  Which of the following is true about the interaction with the (lamda) reppressor and RNA polymerase at P (RM)? 
A.
B.
C.
D.
E.
42.  Which of the following is true about the cro gene? 
A.
B.
C.
D.
E.
43.  During an experiment to study the rate of infection of bacteria with T4, the bacteria with T4, the bacterial cultures were accidentally exposed to a UV light source. Which of the following would be expected for this exposure? 
A.
B.
C.
D.
E.
44.  You were asked to choose a method to separate a mixture of eukaryotic polymerases. Which of the following methods would you choose? 
A.
B.
C.
D.
E.
45.  Why three RNA polymerases? How many types of RNA in the cells? 
A.
B.
C.
D.
E.
46.  Which of the following are products of RNA polymerase II activity? 
A.
B.
C.
D.
E.
47.  Which of the following products are made by RNA polymerase III? 
A.
B.
C.
D.
E.
48.  Which of the following statements about ribosomal genes is incorrect? 
A.
B.
C.
D.
E.
49.  An antibody was produced and used in an immunohistochemical assay to detect the location of RNA polymerase I in a thin section of a liver biopsy. The section was antibody-labeled with a fluorescent tag, then viewed under a fluorescent microscope. State where you would expect to find positive staining if the enzymes is present. 
A.
B.
C.
D.
50.  A new mutant cell line was developed and was found to be defective in polymerase III activity. Which of the following is likely to be observed in this cell line? 
A.
B.
C.
D.
E.
51.  An experiment is planned to study the effect of reducing the transcription of the myosin gene. Which of the following inhibitiors wold be useful in this study? 
A.
B.
C.
D.
E.
52.  Which of the following would you choose to study the role of polymerase subunits in elongation? 
A.
B.
C.
D.
E.
53.  Which of the following would you choose to specifically collect polymerase subunits in cell extracts? 
A.
B.
C.
D.
E.
54.  Which of the following is not a part of the core class II promoter? 
A.
B.
C.
D.
E.
55.  Which of the following techniques would you use to study the role of the BRE? 
A.
B.
C.
D.
E.
56.  Which statement is wrong regarding promoter in humans? 
A.
B.
C.
D.
E.
57.  Predict a possible effect of deleting the enhancers region of the polymerase I gene. 
A.
B.
C.
D.
E.
58.  Eukaryotic ribosomal RNA gene are transcribed by RNA polymerase II
A.
B.
59.  Roeder and Rutter showed that there are four polymerases operating in the eukaryotic cell 
A.
B.
60.  Epitope taggin is an efficicent way to immunoprecipate to isolate an entire polymerase complex. 
A.
B.
61.  Which of the following is the correct order of addition of general transcription factors to the initiation complex? 
A.
B.
C.
D.
E.
62.  An electrophoretic mobility shift assay (EMSA) was conducted to check for the binding of TFIIB to a segment of a DNA promoter region. Which of the following approaches could be used to confirm that TFIIB is indeed binding? 
A.
B.
C.
D.
E.
63.  You are planning an experiment to look at the specific binding of the TBP to the promoter. Which of the following would be a suitable negative control for this experiment? 
A.
B.
C.
D.
E.
64.  Which of the following would you expect to bind to a chromatography column made of a resin conjugated to UBF? 
A.
B.
C.
D.
E.
65.  Determine which of the following would most likely lead to a dramatic reduction in transcription. 
A.
B.
C.
D.
E.
66.  TFIID collaborates with TFIIA to bind to the TATA box.
A.
B.
67.   RNA polymerase prevents the binding of TFIIF to the promoter region
A.
B.
68.  A promoter region replacing the TATA box with the ICIC box will not bind to TFIID. 
A.
B.
69.  Which of the following are typical features of transcriptional activators? 
A.
B.
C.
D.
E.
70.  Which of the following can be found in DNA-binding domains? 
A.
B.
C.
D.
E.
71.  Select the feature that is not true of the GAL4 protein. 
A.
B.
C.
D.
E.
72.  Which of the following is a target site for GAL4?
A.
B.
C.
D.
E.
73.  One effect of mutation or deleting the activation domain of a transcriptional activator would be 
A.
B.
C.
D.
E.
74.  The glucocorticoid receptor is activated by binding to 
A.
B.
C.
D.
E.
75.  Which of the following is a reasonable way to explain the ability of enhancers to act at a distance? 
A.
B.
C.
D.
E.
76.  HMG (Y) plays a role in IFN-(beta) transcription by
A.
B.
C.
D.
E.
77.  Insulators are DNA elements that 
A.
B.
C.
D.
E.
78.  Which of the following histone protein is the most highly conserved from one organism to another? 
A.
B.
C.
D.
E.
79.  Which of the following is the correct order of chromatin folding? 
A.
B.
C.
D.
E.
80.  Studying Xenopus transcription of somatic and oocyte 5s-rRNA genes, Donald Browna nd colleagues demonstrated that the difference in the expression patterns in the two types of cell could be seen as a race between 
A.
B.
C.
D.
E.
81.  One method to demonstrate existence of nucleosome free DNA regions is 
A.
B.
C.
D.
E.
82.  Histone acetylation can occur in the 
A.
B.
C.
D.
E.
83.  The Histone Code states that the 
A.
B.
C.
D.
E.
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