Quiz Online Uas Biologi Molekuler Farmasi 2008/2009
DIRECTIONS: Each question below contains four suggested responses. Please choose by clicking the one best response to each question.Ketentuan:1. Quiz online akan dilaksanakan pada Hari Sabtu, 20-6-09, Jam 14.00 s.d 16.00 WIB2. Tulis empat digit terakhir NIM saudara pada kotak "your name"2. Pemenang adalah yang memperoleh nilai tertinggi dalam jangka waktu 60 menitNote:Password quiz akan dihapus beberapa saat sebelum pelaksanaan quiz ini.
- 1.
The term refers to the population of mRNA at any given point in a cell’s life.
- A.
Genome
- B.
Exon
- C.
Proteome
- D.
Transcriptome
Correct Answer
D. Transcriptome -
- 2.
Which of the following is NOT a component of nucleotide of RNA?
- A.
Phosphodiester bond
- B.
Hydrogen bond
- C.
Hydrophobic
- D.
β-N-glycosidic bond
Correct Answer
B. Hydrogen bond -
- 3.
The nucleotide sequence to which a repressor protein binds to prevent transcription of a gene or operon
- A.
2′-deoxyribose
- B.
A nitrogenous base
- C.
A phosphate group
- D.
Ribose
Correct Answer
A. 2′-deoxyribose -
- 4.
Which of the following is an accurate statement concerning the differences between DNA and RNA?
- A.
Promoter
- B.
Repressor
- C.
Operator
- D.
Open reading frame (ORF)
Correct Answer
C. Operator -
- 5.
A coding region within a discontinuous gene.
- A.
RNA is usually double-stranded, but DNA is usually single-stranded.
- B.
RNA lacks the base thymine (which is found in DNA) and has uracil instead
- C.
RNA contains three different nucleotides, but DNA contains four different nucleotides.
- D.
RNA has the sugar deoxyribose, but DNA has the sugar ribose
Correct Answer
B. RNA lacks the base thymine (which is found in DNA) and has uracil instead -
- 6.
The nucleotide sequence, upstream of a gene, to which RNA polymerase binds in order to initiate transcription
- A.
Exon
- B.
Operon
- C.
Codon
- D.
Promoter
Correct Answer
A. Exon -
- 7.
The flow of genetic information is from DNA to RNA to protein, via the processes of transcription and translation. This concept is known as ____________
- A.
Operator
- B.
Operon
- C.
Enhancer
- D.
Promoter
Correct Answer
D. Promoter -
- 8.
The basic unit of organisation in biological systems.
- A.
The central dogma
- B.
Operon
- C.
DNA cloning
- D.
Genetic Engineering
Correct Answer
A. The central dogma -
- 9.
Following cell disruption, cell preparations can be deproteinised by using __________
- A.
Gene
- B.
Operon
- C.
Codon
- D.
Cell
Correct Answer
D. Cell -
- 10.
In the gene cloning technique, the enzyme _____________ is used to breaks phosphodiester bonds within a nucleic acid molecule.
- A.
CaCl2
- B.
RNase
- C.
Ethanol
- D.
Phenol
Correct Answer
D. Phenol -
- 11.
All of the following are the basic mechanis steps of PCR, EXCEPT_________
- A.
DNA polymerase
- B.
Polynucleotide kinase
- C.
Ligase
- D.
Endonuclease
Correct Answer
D. Endonuclease -
- 12.
Which of the following is a technique that is carried out by placing the nucleic acid samples in the gel and applying a potential difference across it.
- A.
Duplex DNA is heat denatured to give single strands
- B.
The two oligonucleotide primers are annealed to the target DNA
- C.
A thermostable DNA polymerase adds dNTPs onto the 3' ends of the two annealed primers
- D.
Detecting fragments in an agarose gel that are complementary to a given DNA sequence
Correct Answer
D. Detecting fragments in an agarose gel that are complementary to a given DNA sequence -
- 13.
Separation of the DNA fragments created in sequencing reactions is achieved by ________.
- A.
PCR
- B.
SAGE
- C.
PAGE
- D.
DNA sequencing
Correct Answer
B. SAGE -
- 14.
Which of the following enzymes are found in bacterial cells, where they act as a ‘protection’ system for bacteria in that they hydrolyse exogenous DNA that is not methylated by the host modification enzyme?
- A.
PCR . .
- B.
SAGE
- C.
PAGE
- D.
Northern Blotting
Correct Answer
C. PAGE -
- 15.
A high-capacity cloning vector consisting of the λ cos site inserted into a plasmid.
- A.
Restriction enzyme
- B.
Alkaline phosphatase
- C.
Reverse transcriptase
- D.
DNA Ligase
Correct Answer
A. Restriction enzyme -
- 16.
Which of the following is the enzyme that is used mainly for copying mRNA molecules in the preparation of cDNA (complementary or copy DNA) for cloning?
- A.
PB322
- B.
Virus
- C.
Plasmid
- D.
Cosmid
Correct Answer
D. Cosmid -
- 17.
Which of the following is an enzyme that removes phosphate groups from the 5′ ends of DNA molecules.
- A.
Restriction enzyme
- B.
Alkaline phosphatase
- C.
Reverse transcriptase
- D.
DNA Ligase
Correct Answer
C. Reverse transcriptase -
- 18.
An enzyme that adds one or more nucleotides to the 3′ end of a DNA molecule.
- A.
Restriction enzyme
- B.
Alkaline phosphatase
- C.
Reverse transcriptase
- D.
Terminal transferase
Correct Answer
B. Alkaline phosphatase -
- 19.
An enzyme that synthesizes phosphodiester bonds as part of DNA replication, repair and recombination processes.
- A.
Restriction enzyme
- B.
DNA Ligase
- C.
Alkaline phosphatase
- D.
Terminal transferase
Correct Answer
D. Terminal transferase -
- 20.
A cell that has been introduced by exogenous DNA
- A.
Restriction enzymes
- B.
Alkaline phosphatase
- C.
Reverse transcriptase
- D.
DNA Ligase
Correct Answer
D. DNA Ligase -
- 21.
Transformation of competent cells is carried out by mixing the plasmid DNA with the cells, incubating on ice for 20--30 min, and then giving a brief heat shock at __________.
- A.
Transformant
- B.
DNA recombinant
- C.
E. coli recombinant
- D.
Plasmid recombinant
Correct Answer
A. Transformant -
- 22.
Which of the following is a vector that can be used to clone large fragments of DNA (in excess of several hundred kb).
- A.
42◦C
- B.
52◦C
- C.
72◦C
- D.
92◦C
Correct Answer
A. 42◦C -
- 23.
Which of the following is plasmid that are found in Agrobacterium tumifaciens and are responsible for the formation of crown gall tumors in plants?
- A.
PUC18
- B.
Yip
- C.
Ti Plasmid
- D.
BAC
Correct Answer
D. BAC -
- 24.
Using mRNA as the template is a common variant of the basic PCR, this technique known as _________.
- A.
PUC18
- B.
Yip
- C.
Ti Plasmid
- D.
BAC
Correct Answer
C. Ti Plasmid -
- 25.
The conversion of RNA to DNA requires a special enzyme called ______________
- A.
Nested PCR
- B.
Inverse PCR
- C.
RAPD-PCR
- D.
RT-PCR
Correct Answer
D. RT-PCR -
- 26.
Which of the following is often used to describe a set of clones representing the entire genome of an organism?
- A.
Transcriptase
- B.
RNA-dependent DNA polymerase
- C.
DNA polymerase
- D.
Template-dependent DNA polymerase
Correct Answer
B. RNA-dependent DNA polymerase -
- 27.
A useful method for genomic profiling and involves using a single primer at low stringency.
- A.
Genomics
- B.
Proteome
- C.
Genomic library
- D.
CDNA library
Correct Answer
C. Genomic library -
- 28.
A vector that can replicate in the cells of more than one organism (e.g. in Escherichia coli and in yeast).
- A.
Nested PCR
- B.
Inverse PCR
- C.
RAPD-PCR
- D.
RT-PCR
Correct Answer
C. RAPD-PCR -
- 29.
Which of the following is plasmid that contains genes for ampicillin resistance (Apr) and tetracycline resistance (Tcr)
- A.
Cosmid
- B.
Shuttle vector
- C.
Phagemid
- D.
Yip
Correct Answer
B. Shuttle vector -
- 30.
If nucleic acid samples are not subjected to electrophoresis but are spotted onto the filters, the technique is known as ___________
- A.
PUC18
- B.
PBR322
- C.
YEp
- D.
Yip
Correct Answer
B. PBR322 -
- 31.
A technique used for detection of a specific RNA molecule against a background of many other RNA molecules.
- A.
Southern hybridization
- B.
Northern hybridization
- C.
Dot blotting
- D.
Western hybridization
Correct Answer
C. Dot blotting -
- 32.
This technique involves the transfer of electrophoresed protein bands from a polyacrylamide gel on to a membrane of nitrocellulose or nylon.
- A.
Southern hybridization
- B.
Northern hybridization
- C.
PCR
- D.
Western hybridization
Correct Answer
B. Northern hybridization -
- 33.
Which of the following is a colourless substrate for β-galactosidase.
- A.
Southern hybridization
- B.
Northern hybridization
- C.
PCR
- D.
Western hybridization
Correct Answer
D. Western hybridization -
- 34.
Which of the following is a highly stable synthetic analog of lactose
- A.
Chromogenic substrate
- B.
X-gal
- C.
LacZ
- D.
IPTG
Correct Answer
B. X-gal -
- 35.
A technique that results in exponential amplification of a selected region of a DNA molecule.
- A.
Chromogenic substrate
- B.
X-gal
- C.
LacZ
- D.
IPTG
Correct Answer
D. IPTG -
- 36.
Which of the following is a synthetic, double-stranded oligonucleotide used to attach sticky ends to a blunt-ended molecule.
- A.
Southern hybridization
- B.
Northern hybridization
- C.
PCR
- D.
Western hybridization
Correct Answer
C. PCR -
- 37.
Which of the following is a short, synthetic, single-stranded oligonucleotide used in DNA sequencing or PCR
- A.
Primer
- B.
Linker
- C.
Selectable marker
- D.
Template
Correct Answer
B. Linker -
- 38.
Which of the following is a gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector.
- A.
Primer
- B.
Linker
- C.
Selectable marker
- D.
Template
Correct Answer
A. Primer -
- 39.
A single-stranded polynucleotide (or region of a polynucleotide) that directs synthesis of a complementary polynucleotide.
- A.
Primer
- B.
Linker
- C.
Selectable marker
- D.
Template
Correct Answer
C. Selectable marker -
- 40.
A cloning strategy that involves the insertion of random fragments of a large DNA molecule into a vector, resulting in a large number of different recombinant DNA molecules.
- A.
Primer
- B.
Linker
- C.
Selectable marker
- D.
Template
Correct Answer
D. Template -
- 41.
A cloning strategy whereby insertion of a new piece of DNA into a vector inactivates a gene carried by the vector.
- A.
Shotgun cloning
- B.
CDNA cloning
- C.
Insertional inactivation
- D.
DNA cloning
Correct Answer
A. Shotgun cloning -
- 42.
A technique was invented by Kary Mullis and his colleagues in the 1980s.
- A.
Shotgun cloning
- B.
CDNA cloning
- C.
Insertional inactivation
- D.
DNA cloning
Correct Answer
C. Insertional inactivation -
- 43.
A method used to indentify the polypeptide coded by a cloned gene which blocks the mRNA and prevents its translation when placed in a system containing all the components of the translational machinery.
- A.
Southern hybridization
- B.
Northern hybridization
- C.
PCR
- D.
Western hybridization
Correct Answer
C. PCR -
- 44.
A method used to indentify the polypeptide coded by a cloned gene which is immobilised and used to select the clone-specific mRNA from the total mRNA preparation.
- A.
Hybrid-arrest translation
- B.
Hybrid-release translation
- C.
Insertional inactivation
- D.
Hybrid-select translation
Correct Answer
A. Hybrid-arrest translation -
- 45.
The introduction of purified virus DNA molecules into any living cell.
- A.
Hybrid-arrest translation
- B.
Hybrid-release translation
- C.
Insertional inactivation
- D.
Hybrid-select translation
Correct Answer
B. Hybrid-release translation -
- 46.
A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA
- A.
Vector
- B.
Electroporation
- C.
Transformation
- D.
Transfection
Correct Answer
D. Transfection -
- 47.
A technique which involves the transfer of electrophoretically separated protein molecules to membranes
- A.
Vector
- B.
Primer
- C.
Linker
- D.
Selectable marker
Correct Answer
A. Vector -
- 48.
Which of the following is a technique for determining the order of nucleotides in a DNA molecule
- A.
Southern hybridization
- B.
Northern hybridization
- C.
PCR
- D.
Western hybridization
Correct Answer
D. Western hybridization -
- 49.
Insertion of a fragment of DNA into a cloning vector, and subsequent propagation of the recombinant DNA molecule in a host organism.
- A.
PCR
- B.
SAGE
- C.
PAGE
- D.
DNA sequencing
Correct Answer
D. DNA sequencing -
- 50.
Insertion of a fragment of DNA into a cloning vector, and subsequent propagation of the recombinant DNA molecule in a host organism.
- A.
Shotgun cloning
- B.
CDNA cloning
- C.
Insertional inactivation
- D.
DNA cloning
Correct Answer
D. DNA cloning -
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Mar 20, 2022Quiz Edited by
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Jun 18, 2009Quiz Created by
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