Protein Sequencing

10 Questions | By Karthi_dr | Updated: Feb 24, 2015 | Attempts: 203

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1.

First protein that was sequenced by sanger
- A.
  
  Human Insulin
- B.
  
  Bovine insulin
- C.
  
  Porcine insulin
- D.
  
  Feline insulin
Correct Answer
B. Bovine insulin

Explanation
Sanger sequenced DNA also. He got 2 Nobel prizes.

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2.

Sanger's reagent is
- A.
  
  Dinitrofluro benzene
- B.
  
  Phenyl isothiocyanate
- C.
  
  Performic acid
- D.
  
  Iodoacetate
Correct Answer
A. Dinitrofluro benzene
3.

Edman's reagent is
- A.
  
  Dinitrofluro benzene
- B.
  
  Phenyl isothiocyanate
- C.
  
  Performic acid
- D.
  
  Iodoacetate
Correct Answer
B. Phenyl isothiocyanate
4.

Dansyl chloride is used to
- A.
  
  Determine the N-terminal amino acid
- B.
  
  Determine the C-terminal amino acid
- C.
  
  Protect the SH residues
- D.
  
  None
Correct Answer
A. Determine the N-terminal amino acid
5.

Cyanogen bromide cleaves
- A.
  
  Carboxy terminal methionine residues
- B.
  
  Amino terminal methionine residues
- C.
  
  Internal methionine residues
- D.
  
  None
Correct Answer
C. Internal methionine residues
6.

Advantage(s) of mass sepectrometry based sequencing
- A.
  
  Glycosylation can be identified
- B.
  
  Pyroglutamate can be identified
- C.
  
  Phosphorylation can be identified
- D.
  
  All of the above
Correct Answer
D. All of the above
7.

Reverse sequencing is
- A.
  
  Sequencing from carboxy terminal
- B.
  
  Getting amino acid sequences form DNA sequences
- C.
  
  Either of the above
- D.
  
  None of the above
Correct Answer
B. Getting amino acid sequences form DNA sequences

Explanation
Reverse sequencing is always confirmed by protein sequencing.

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8.

Detectors used in mass spectrometry detects
- A.
  
  Mass
- B.
  
  Charge
- C.
  
  Mass/charge ratio
- D.
  
  None
Correct Answer
C. Mass/charge ratio

Explanation
m/z ratio

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9.

Protein denaturing agent(s)
- A.
  
  Urea
- B.
  
  Guanidine hydrochloride
- C.
  
  Either of the above
- D.
  
  None of the above
Correct Answer
C. Either of the above
10.

True about solid phase peptide synthesis
- A.
  
  N-terminal of growing peptide chain is anchored
- B.
  
  C-terminal of the incoming amino acid is protected
- C.
  
  No need to block reactive side chain groups
- D.
  
  None
Correct Answer
D. None

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Current Version
Feb 24, 2015

Quiz Edited by
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Feb 23, 2015

Quiz Created by
Karthi_dr

Protein Sequencing

First protein that was sequenced by sanger

Sanger's reagent is

Edman's reagent is

Dansyl chloride is used to

Cyanogen bromide cleaves

Advantage(s) of mass sepectrometry based sequencing

Reverse sequencing is

Detectors used in mass spectrometry detects

Protein denaturing agent(s)

True about solid phase peptide synthesis