Protein Sequencing

Question 1 / 11

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1. Edman's reagent is

Dinitrofluro benzene

Phenyl isothiocyanate

Performic acid

Iodoacetate

About This Quiz

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2. Advantage(s) of mass sepectrometry based sequencing

Glycosylation can be identified

Pyroglutamate can be identified

Phosphorylation can be identified

All of the above

3. Detectors used in mass spectrometry detects

Mass

Charge

Mass/charge ratio

None

m/z ratio

Explanation

m/z ratio

4. Dansyl chloride is used to

Determine the N-terminal amino acid

Determine the C-terminal amino acid

Protect the SH residues

None

5. Sanger's reagent is

Dinitrofluro benzene

Phenyl isothiocyanate

Performic acid

Iodoacetate

6. First protein that was sequenced by sanger

Human Insulin

Bovine insulin

Porcine insulin

Feline insulin

Sanger sequenced DNA also. He got 2 Nobel prizes.

Explanation

Sanger sequenced DNA also. He got 2 Nobel prizes.

7. Protein denaturing agent(s)

Urea

Guanidine hydrochloride

Either of the above

None of the above

8. Reverse sequencing is

Sequencing from carboxy terminal

Getting amino acid sequences form DNA sequences

Either of the above

None of the above

Reverse sequencing is always confirmed by protein sequencing.

Explanation

Reverse sequencing is always confirmed by protein sequencing.

9. Cyanogen bromide cleaves

Carboxy terminal methionine residues

Amino terminal methionine residues

Internal methionine residues

None

10. True about solid phase peptide synthesis

N-terminal of growing peptide chain is anchored

C-terminal of the incoming amino acid is protected

No need to block reactive side chain groups

None

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