X-ray Crystallography: Data Processing Summary Quiz
- 1.What must we do to our crystal to collect the various reflections we need to analyse our protein?
- A.
Rotate it
- B.
Nothing
- C.
Use computer software to predict these other reflections
- D.
Look at the signal:noise ratio to separate these other reflections from background noise
- 2.Mosflm is one of the many statistics we use analyse the quality of our data.
- A.
True
- B.
False
- 3.The number of unique reflections is the total number of reflections measured during an experiment.
- A.
True
- B.
False
- 4.What must you do before analysing your data statistically?
- A.
Decide on a likely lattice type and unit cell size
- B.
Calculate amplitudes of diffracted waves
- C.
Identify and get rid of any noise in the diffraction data
- D.
Determine the asymmetric unit and the unit cell size
- 5.Why do we measure the intensity of our diffraction spots?
- A.
To give us a list of reflection intensities we can compare
- B.
To give us statistical information
- C.
To help determine the lattice type and space group
- D.
To give an indication of the quality of our data
- 6.Deciding on a unit cell size is part of the first step of processing your data.
- A.
True
- B.
False
- 7.Which of the following is an indication of data quality?
- A.
Whether spots are where you expect them to be
- B.
R-Factor
- C.
The list of spot intensities
- D.
R-Free
- 8.Why do we get two values for each statistic?
- A.
One for all the data, and one for those at the edge of the detector
- B.
One for inner reflections, and one for outer reflections
- C.
One for high and low resolution data
- 9.Some statistical information can be gathered, before the processing of diffraction data.
- A.
True
- B.
False
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