X-ray Crystallography: Data Processing Summary Quiz

Reviewed by Editorial Team
The ProProfs editorial team is comprised of experienced subject matter experts. They've collectively created over 10,000 quizzes and lessons, serving over 100 million users. Our team includes in-house content moderators and subject matter experts, as well as a global network of rigorously trained contributors. All adhere to our comprehensive editorial guidelines, ensuring the delivery of high-quality content.
Learn about Our Editorial Process
| By Ae342
A
Ae342
Community Contributor
Quizzes Created: 1 | Total Attempts: 363
| Attempts: 363 | Questions: 10
Please wait...
Question 1 / 10
0 %
0/100
Score 0/100
1. The number of unique reflections is the total number of reflections measured during an experiment.

Explanation

The statement is false because the number of unique reflections is not the total number of reflections measured during an experiment. Unique reflections refer to the distinct or different reflections that are observed, which may be fewer in number than the total reflections measured. This is because some reflections may be repeated or overlapping, leading to a smaller count of unique reflections.

Submit
Please wait...
About This Quiz
Biochemistry Quizzes & Trivia

This quiz on X-Ray Crystallography: Data Processing assesses understanding of crystal rotation, data analysis tools like Mosflm, reflection measurement, and statistical data analysis preparation. It's designed for learners to grasp essential techniques in protein structure analysis.

Tell us your name to personalize your report, certificate & get on the leaderboard!
2. Deciding on a unit cell size is part of the first step of processing your data.

Explanation

Deciding on a unit cell size is indeed part of the first step of processing data. In crystallography, a unit cell is the smallest repeating unit of a crystal lattice. Determining the appropriate unit cell size is crucial for accurately analyzing and interpreting the data obtained from crystallographic experiments. By defining the unit cell dimensions, scientists can calculate important parameters such as lattice constants, symmetry elements, and atomic positions. Therefore, it is correct to say that deciding on a unit cell size is an essential step in the data processing stage.

Submit
3. What is the desirable types of values for each of the statistics below?
Submit
4. What must we do to our crystal to collect the various reflections we need to analyse our protein?

Explanation

To collect the various reflections needed to analyze the protein, we must rotate the crystal. This is because rotating the crystal allows different angles of the crystal lattice to be exposed to the X-ray beam, resulting in different reflections. By rotating the crystal, we can obtain a wider range of reflections, which helps in the analysis of the protein structure.

Submit
5. Some statistical information can be gathered, before the processing of diffraction data.

Explanation

The statement suggests that statistical information can be collected before processing diffraction data. However, this is incorrect. Statistical information is typically gathered after the processing of diffraction data, as it involves analyzing the data and drawing conclusions based on the results obtained. Therefore, the correct answer is False.

Submit
6. Mosflm is one of the many statistics we use analyse the quality of our data.

Explanation

Mosflm is not a statistic but rather a software program used for processing diffraction data from macromolecular crystals. It is not used to analyze the quality of data but rather to determine the orientation and intensity of the diffracted X-rays. Therefore, the statement is false.

Submit
7. Why do we get two values for each statistic?

Explanation

The reason we get two values for each statistic is because we want to analyze the data from different perspectives. One value represents the overall data, giving us a general understanding of the statistic. The other value specifically focuses on the data at the edge of the detector, which might have different characteristics or be subject to different conditions. By comparing these two values, we can gain insights into the variations and potential biases in the data, helping us to make more accurate interpretations and conclusions.

Submit
8. Which of the following is an indication of data quality?

Explanation

An indication of data quality can be determined by whether the spots are where you expect them to be. This suggests that the data is accurate and reliable, as the expected spots align with the actual spots observed. If the spots are not where they are expected to be, it may indicate errors or inconsistencies in the data, which can affect the overall quality and reliability of the information.

Submit
9. What must you do before analysing your data statistically?

Explanation

Before analyzing the data statistically, it is important to decide on a likely lattice type and unit cell size. This is because the lattice type and unit cell size determine the symmetry and arrangement of the crystal structure, which is crucial for further analysis. Additionally, calculating the amplitudes of diffracted waves is necessary as it provides information about the intensity of the diffraction pattern, which is essential for statistical analysis. These steps help in accurately interpreting and understanding the data before performing statistical analysis.

Submit
10. Why do we measure the intensity of our diffraction spots?

Explanation

We measure the intensity of our diffraction spots to give us a list of reflection intensities that we can compare. This allows us to analyze the diffraction pattern and determine the relative strengths of different reflections. By comparing the intensities, we can gain statistical information about the crystal structure, such as the arrangement of atoms in the lattice. Additionally, the intensity measurements can help us determine the lattice type and space group, providing further information about the crystal's symmetry and arrangement of atoms. Furthermore, the intensity measurements can also give us an indication of the quality of our data, helping us assess the accuracy and reliability of our experimental results.

Submit
View My Results

Quiz Review Timeline (Updated): Mar 19, 2023 +

Our quizzes are rigorously reviewed, monitored and continuously updated by our expert board to maintain accuracy, relevance, and timeliness.

  • Current Version
  • Mar 19, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • Nov 17, 2013
    Quiz Created by
    Ae342
Cancel
  • All
    All (10)
  • Unanswered
    Unanswered ()
  • Answered
    Answered ()
The number of unique reflections is the total number of reflections...
Deciding on a unit cell size is part of the first step of processing...
What is the desirable types of values for each of the statistics...
What must we do to our crystal to collect the various reflections we...
Some statistical information can be gathered, before the processing of...
Mosflm is one of the many statistics we use analyse the quality of our...
Why do we get two values for each statistic?
Which of the following is an indication of data quality?
What must you do before analysing your data statistically?
Why do we measure the intensity of our diffraction spots?
Alert!

Advertisement