Micro Exam 3
Summer Micro Test 3 study quiz for the final.
- 1.
Among the microorganisms, various genomes can include
- A.
Chromosomes
- B.
Plasmids
- C.
Mitochondrial DNA
- D.
Chloroplast DNA
- E.
All of the choices are correct
Correct Answer
E. All of the choices are correct -
- 2.
Eukaryotic chromosomes differ from prokaryotic chromosomes because only eukaryotes have
- A.
Histone proteins
- B.
Chromosomes in a nucleus
- C.
Several to many chromosomes
- D.
Elongated, not circular, chromosomes
- E.
All of the above
Correct Answer
E. All of the above -
- 3.
The antiparralel arrangement within DNA molecules refers to
- A.
Each base bonding at the 1' position of the sugar
- B.
A purine always bonding to a pyrimidine
- C.
One helix strand that runs from 5' to 3' direction and the other strand runs from the 3' to 5' direction
- D.
An original parent DNA strand and one newly synthesized DNA strand comprising a new DNA molecule
- E.
None of the above
Correct Answer
C. One helix strand that runs from 5' to 3' direction and the other strand runs from the 3' to 5' direction -
- 4.
Which enzyme fills in the spaces between the Okazaki fragments with the correct nucleotide
- A.
DNA ligase
- B.
DNA polymerases
- C.
DNA helicases
- D.
DNA gyrases
- E.
Primases
Correct Answer
A. DNA ligase -
- 5.
The enzymes that can proofread replicating DNA, detect incorrect bases, excise them, and correctly replace them are
- A.
DNA ligases
- B.
DNA polymerases
- C.
DNA helicases
- D.
DNA gyrases
- E.
Primases
Correct Answer
B. DNA polymerases -
- 6.
The RNA molecules that carry amino acids to the ribosomes during protein synthesis are called
- A.
Ribosomal RNA
- B.
Messenger RNA
- C.
Transfer RNA
- D.
Primer RNA
- E.
Ribozymes
Correct Answer
C. Transfer RNA -
- 7.
This molecule is transcribed from the DNA template strand and later translated
- A.
Ribosomal RNA
- B.
Messenger RNA
- C.
Transfer RNA
- D.
Primer RNA
- E.
Protein
Correct Answer
B. Messenger RNA -
- 8.
RNA molecules differ from DNA molecules because only RNA
- A.
Has ribose
- B.
Has uracil
- C.
Is typically one strand of nucleotides
- D.
Does not have thymine
- E.
All of the above are correct
Correct Answer
E. All of the above are correct -
- 9.
If a codon for alanine is GCA, then the anitcodon is
- A.
GCA
- B.
CGT
- C.
ACG
- D.
CGU
- E.
UGC
Correct Answer
D. CGU -
- 10.
Which is incorrect about inducible operons
- A.
They have genes turned offf by a buildup of end product
- B.
They are often for catabolic pathways
- C.
They are normally turned off
- D.
They are turned on by the substate of the enzyme
- E.
They include the lac operon
Correct Answer
A. They have genes turned offf by a buildup of end product -
- 11.
A mutation that changes a normal codon to a stop codon is called a
- A.
Point mutation
- B.
Silent mutation
- C.
Back mutation
- D.
Missense mutation
- E.
Nonsense mutation
Correct Answer
E. Nonsense mutation -
- 12.
T/F Conjugation is that act at which two or more eukaryotic cells come together and exchange DNA. All cells must have a unique pilus in order for the cells to bind.
- A.
True
- B.
False
Correct Answer
B. False -
- 13.
This test is used to measure a chemicals capacity to cause mutation
- A.
Amos test
- B.
Griffith Test
- C.
Ames Test
- D.
Susceptibility test
- E.
The bar-bar test
Correct Answer
C. Ames Test -
- 14.
The use of an organism's biochemical processes to crate a product is
- A.
Genetic engineering
- B.
Biotechnology
- C.
Recombinant dna
- D.
Gel elctrophoreses
- E.
Gene probes
Correct Answer
B. Biotechnology -
- 15.
The various techniquest by which scientist manipulate DNA in the lab are called
- A.
Genetic engineering
- B.
Biotechnology
- C.
Recominant DNA
- D.
Gel electrophoresis
- E.
Gene probes
Correct Answer
A. Genetic engineering -
- 16.
A technique that separates a readable pattern of DNA fragment is
- A.
Genetic engineering
- B.
Biotechnology
- C.
Recombinant DNA
- D.
Gel electrophoresis
- E.
Gene probes
Correct Answer
D. Gel electrophoresis -
- 17.
EcoRI and HindIII are
- A.
Palindromes
- B.
Reverse transcriptase
- C.
Restriction endonucleases
- D.
Ligases
- E.
DNA polymerases
Correct Answer
C. Restriction endonucleases -
- 18.
Sequences to DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are
- A.
Palindrones
- B.
Reverse transcriptases
- C.
Restriction endonucleases
- D.
Ligases
- E.
DNA polymerases
Correct Answer
A. Palindrones -
- 19.
Analysis of DNA fragments in gel electrophoresis involves
- A.
Larger fragments move slowly and remain closer to the wells
- B.
DNA has an overall negative charge and moves to the positive pole
- C.
DNA fragments are stained to see them
- D.
An electric current through the gel causes DNA fragments to migrate
- E.
All of the above
Correct Answer
E. All of the above -
- 20.
The Western Blot technique detects
- A.
DNA
- B.
RNA
- C.
Proteins
- D.
Recombinant DNA
- E.
Specific gentic marker sequence on genes
Correct Answer
C. Proteins -
- 21.
Ethidium bromide and malachite green do what to DNA
- A.
Are known carcinogens because they cause theymine dimmers
- B.
Are known carcinogens because they intercalate with DNA
- C.
Are known carcinogens because they inhibit repair enzyme
- D.
Are just mutagens
Correct Answer
B. Are known carcinogens because they intercalate with DNA -
- 22.
DNA polymerases used in PCR
- A.
Use an RNA template to make complementary DNA
- B.
Must remain active at very cold temperatures
- C.
Include Taq polymerase
- D.
Are labeled with fluorescent dyes
- E.
All of the above
Correct Answer
C. Include Taq polymerase -
- 23.
The Southern Blot technique detects
- A.
DNA
- B.
RNA
- C.
Proteins
- D.
Recombinant DNA
- E.
Specific genetic marker sequence on genes
Correct Answer
A. DNA -
- 24.
The polymersase chain reaction (PCR) has been used in all of the following fields except
- A.
Forensics
- B.
Evolutionary studies
- C.
Gene mapping
- D.
Medicine
- E.
None of the choices, it is usefull in all of these fields
Correct Answer
E. None of the choices, it is usefull in all of these fields -
- 25.
T/F Reverse transcriptase is used to make cDNA from an RNA template
- A.
True
- B.
False
Correct Answer
A. True -
- 26.
T/F Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid
- A.
True
- B.
False
Correct Answer
A. True -
- 27.
The rate of migration of macromolecules in gel electrophoresis depends on what characteristics
- A.
Concentration of agarose gel
- B.
The voltage
- C.
Size of DNA fragment
- D.
Only a and b
- E.
All of the above
Correct Answer
E. All of the above -
- 28.
T/F Southern blots may be used to probe for specific gene sequences. Thus this is an alternative tool to use to ensure a host cell picked up the donor plasmid
- A.
True
- B.
False
Correct Answer
A. True -
- 29.
T/F It is important that an ideal vector have a point of replication and have relatively large genomes
- A.
True
- B.
False
Correct Answer
B. False -
- 30.
Physical agents for controlling microbial growth include all of the following except
- A.
Ultraviolet radiation
- B.
Boiling water
- C.
HEPA filters
- D.
Pasteurization
- E.
Hydrogen peroxide
Correct Answer
E. Hydrogen peroxide -
- 31.
Which of the following microbial froms have the highest resistance to physical and chemical controls
- A.
Naked viruses
- B.
Porozoan cyst
- C.
Fugal spores
- D.
Bacterial endospores
- E.
Yeast
Correct Answer
D. Bacterial endospores -
- 32.
The use of a physical or chemical process to destroy vegetative pathogens on fomites is
- A.
Disinfection
- B.
Sterilization
- C.
Antisepsis
- D.
Sanitization
- E.
Degermation
Correct Answer
A. Disinfection -
- 33.
The use of chemical agents directly on exposed body surfaces to destroy or inhibit vegatative pathogens is
- A.
Disinfection
- B.
Sterilization
- C.
Antisepsis
- D.
Sanitization
- E.
Degermation
Correct Answer
C. Antisepsis -
- 34.
Sterilization is achieved by
- A.
Flash pasteurization
- B.
Hot water
- C.
Boiling water
- D.
Steam autoclave
- E.
All of the above
Correct Answer
D. Steam autoclave -
- 35.
Dry heat
- A.
Is less efficient than moist heat
- B.
Cannot sterilize
- C.
Includes tyndalliztion
- D.
Is used in devices called autoclaves
- E.
Will sterilize at 121 degrees celcius for 15 minutes
Correct Answer
A. Is less efficient than moist heat -
- 36.
The minimum sterilizing conditions in a steam autoclave are
- A.
121 C at 15 psi for 15 minutes
- B.
63 C for 30 minutes
- C.
160 C for 2 hours
- D.
71.6 C for 15 seconds
- E.
100 C for 30 minutes
Correct Answer
A. 121 C at 15 psi for 15 minutes -
- 37.
Iodophors include
- A.
Chloromines
- B.
Betadine
- C.
Tincture of idodine
- D.
Alcohols
- E.
Chlorhexidine
Correct Answer
B. Betadine -
- 38.
The sterilizing gas used in a special chamber that typically sterilizes soft plastics is
- A.
Ethylend oxide
- B.
Iodophor
- C.
Glutaraldehyde
- D.
Formaldehyde
- E.
Chlorine dioxide
Correct Answer
A. Ethylend oxide -
- 39.
All of the following are methods of disinfection or sterilization except
- A.
Gamma radiation
- B.
Dry oven
- C.
Lyophilization
- D.
Triclosan
- E.
Ethanold
Correct Answer
C. Lyophilization -
- 40.
Subastances that are naturally produced by certain microorganisms that can inhibit or destroy other microorganisms are called
- A.
Antibiotics
- B.
Narrow-spectrum drugs
- C.
Semisynthetic drugs
- D.
Synthetic drugs
- E.
Broad spectrum drugs
Correct Answer
A. Antibiotics -
- 41.
Antimicrobics effective against a wide variety of microbial types are termed
- A.
Antibiotics
- B.
Narrow spectrum drugs
- C.
Semisynthetic drugs
- D.
Synthetic drugs
- E.
Broad spectrum drugs
Correct Answer
E. Broad spectrum drugs -
- 42.
Important characteristics of antimicrobic drugs include
- A.
Readily delivered to the site of infection
- B.
High toxicity against microbial cells
- C.
Do not casue serious side effects in humans
- D.
Remains active in body tissues and fluids
- E.
All of the above
Correct Answer
E. All of the above -
- 43.
Penicillins and cephalosporins
- A.
Interfere with elongation of pertidoglycan
- B.
Are metabolic analogs of PABA and block folic acid synthesis
- C.
Attach to the 30S ribosomal subunit and disrupt protien synthesis
- D.
Damage cell membranes
- E.
Block peptidase that cross-link glycan molecules
Correct Answer
E. Block peptidase that cross-link glycan molecules -
- 44.
Aminoglycosides
- A.
Interfere with elongation of pertidoglycan
- B.
Are metabolic analogs of PABA and block folic acid synthesis
- C.
Attach to the 30S ribosomal subunit and disrupt protien synthesis
- D.
Damage cell membranes
- E.
Block peptidase that cross-link glycan molecules
Correct Answer
C. Attach to the 30S ribosomal subunit and disrupt protien synthesis -
- 45.
Sulfonamides
- A.
Interfere with elongation of pertidoglycan
- B.
Are metabolic analogs of PABA and block folic acid synthesis
- C.
Attach to the 30S ribosomal subunit and disrupt protien synthesis
- D.
Damage cell membranes
- E.
Block peptidase that cross-link glycan molecules
Correct Answer
B. Are metabolic analogs of PABA and block folic acid synthesis -
- 46.
Ampicillin, amoxicillin, mezlocillin, and penicillin G all have
- A.
A beta-lactam ring
- B.
Resistance to the action of penicillinase
- C.
Semisynthetic structure
- D.
Expanded specturm of activity
- E.
All of the above
Correct Answer
A. A beta-lactam ring -
- 47.
Gram negative rods are often treated with
- A.
Penicillin G
- B.
Vancomycin
- C.
Aminoglycosides
- D.
Synercid
- E.
Isoniasid
Correct Answer
C. Aminoglycosides -
- 48.
This drug is used to treat cases of tuberculosis
- A.
Penicillin G
- B.
Vancomycin
- C.
Aminoglycosides
- D.
Synercid
- E.
Isoniasid
Correct Answer
E. Isoniasid -
- 49.
Antimicrobics that are macrolides
- A.
Disrupt cell membrane function
- B.
Include tetracyclines
- C.
Include azithromycin, clarithromycin, and erythromycin
- D.
Are very narrow spectrum
- E.
Are hepatoxic
Correct Answer
C. Include azithromycin, clarithromycin, and erythromycin -
- 50.
An antiviral that is a guanine analog would have an antiviral mode of action that
- A.
Blocks penetration
- B.
Blocks DNA replication
- C.
Inhibits peptidogycan cross linking
- D.
Blocks maturation
- E.
Bonds to ergosterol in cell wall
Correct Answer
B. Blocks DNA replication -
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Jan 16, 2013Quiz Edited by
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Jul 16, 2010Quiz Created by
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