What are the three steps of PCR (in sequential order)? - ProProfs Discuss
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What are the three steps of PCR (in sequential order)?





A. Anneal Primers, Extend DNA, Denature DNA
B. Denature DNA, Extend DNA, Anneal Primers
C. Denature DNA, Anneal Primers, Extend DNA
D. Extend DNA, Anneal Primers, Denature DNA

This question is part of PCR quiz
Asked by Drcoetzer, Last updated: Sep 16, 2019

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2 Answers

A. Lucius

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A. LuciusSenior Content writer, Diploma in Literature, Dover, Delaware
Senior Content writer, Diploma in Literature, Dover, Delaware

Answered on Nov 01, 2019

The correct answer is option C – Denature DNA, Anneal Primers and Extend DNA. Polymerase Chain Reaction (PCR) is a lab technique that occurs in 3 steps. It is used in generating thousands to million copies of a specific section of DNA. In Polymerase Chain Reaction (PCR), there are three major steps. These are the denaturation of DNA, annealing primer, and the extension (elongation) step. In the denaturation step, the temperature is raised to 92 degrees Celsius.

The heat causes the breakdown of hydrogen bonds. In the annealing step, the temperature is lowered to about 50 to 60 degrees Celsius. This will allow primers to attach to specific regions on the DNA through hydrogen bonding. The annealing step takes about 10 to 20 seconds to complete. The elongation step is the final step. The temperature is raised again for the synthesis of new DNA strands.

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drcoetzer

Drcoetzer

Answered on Nov 19, 2017

Denature DNA, Anneal Primers, Extend DNA

PCR relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification
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