Conserved Proteins Quiz

25 Questions  I  By ISci2A18
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  • 1. 
    Which of the following statements is FALSE?
    • A. 

      Sequence-specific protein DNA interactions require contact with the bases of DNA.

    • B. 

      Sequence-independent protein DNA interactions are mostly electrostatic in nature.

    • C. 

      Most sequence-specific protein-DNA interactions involve major groove.

    • D. 

      Promoters are recognized by siRNA.

    • E. 

      Zinc finger domains from the mouse transcription factor Zif268 interact mostly with the major groove on its target DNA.


  • 2. 
    During which of the following processes are RNA-DNA hybrids observed?
    • A. 

      Transcription, prokaryotic only.

    • B. 

      Replication, prokaryotic only.

    • C. 

      Replication, eukaryotic only.

    • D. 

      Replication and transcription, both eukaryotic and prokaryotic.

    • E. 

      Translation, both eukaryotic and prokaryotic.


  • 3. 
    Choose the correct statement.
    • A. 

      Eukaryotic rRNA genes are processed in the nucleolus.

    • B. 

      Eukaryotic genes often contain regions that are transcribed and later excised from the RNA, called exons.

    • C. 

      RNA catalysts are called repressors.

    • D. 

      An AT-rich region found in eukaryotic promoters is called the AUG box.

    • E. 

      Prokaryotic genetic units called introns typically contain genes with related functions.


  • 4. 
    The expressed sequences of eukaryotic pre-mRNAs are known as ____.
    • A. 

      Introns

    • B. 

      Operons

    • C. 

      Exons

    • D. 

      Promoters

    • E. 

      AUG box


  • 5. 
    Most  ____ have well defined 3'ends terminating in poly(A) tails of ~250 nucleotides.
    • A. 

      Prokaryotic mRNAs

    • B. 

      Prokaryotic rRNAs

    • C. 

      Eukaryotic tRNAs

    • D. 

      Eukaryotic mRNAs

    • E. 

      Eukaryotic rRNAs


  • 6. 
    Which of the following statements best describes the role of sigma factors in bacterial gene expression?
    • A. 

      Sigma factors determine which RNA polymerase is used during transcription.

    • B. 

      Sigma factors mediate the excision of intronic sequences.

    • C. 

      A cell's complement of sigma factors determines which of its genes are transcribed.

    • D. 

      Sigma factors bind to the TATA box and initiate transcription.

    • E. 

      None of the rest.


  • 7. 
    In eukaryotes, the three RNA polymerases recognize
    • A. 

      A promoter sequence known as sigma factor.

    • B. 

      A single promoter sequence.

    • C. 

      A promoter sequence known as the Rho factor.

    • D. 

      Different types of promoters.

    • E. 

      None of the rest.


  • 8. 
    How does the preinitiation complex begin to form at a TATA box-containing eukaryotic promoter?
    • A. 

      RNAP binds to the sigma factor.

    • B. 

      RNAP binds to the initiator (Inr) element.

    • C. 

      TATA-binding protein binds to the TATA box.

    • D. 

      RNAP binds to the Rho factor.

    • E. 

      None of the rest.


  • 9. 
    In eukaryotics, mRNAs are synthesized in the cell ____, whereas most translation occurs in the ____.
    • A. 

      Cytosol, nucleosome.

    • B. 

      Cytosol, nucleus.

    • C. 

      Nucleosome, cytosol.

    • D. 

      Nucleus, nucleosome.

    • E. 

      Nucleus, cytosol.


  • 10. 
    Which of the following factors mediate(s) splicing?
    • A. 

      Promoters

    • B. 

      TBP

    • C. 

      SMURFs

    • D. 

      SnRNAs

    • E. 

      TAFs


  • 11. 
    The phosphorylation of the CTD of RNA polymerase II is important for which phase of transcription?
    • A. 

      Initial binding of the promoter

    • B. 

      Conversion from the pre-initiation (closed) complex to the initiation (open) complex

    • C. 

      Progression from initiation to elongation

    • D. 

      Termination of transcription

    • E. 

      None of the rest.


  • 12. 
    RNA interference is a mechanism of posttranscriptional RNA-dependent
    • A. 

      Chromatin-remodeling

    • B. 

      Histone methylation

    • C. 

      Apoptosis

    • D. 

      Gene silencing

    • E. 

      Coactivation


  • 13. 
    Which of the following best describes the functioning of the lac repressor protein?
    • A. 

      In the presence of allolactose, the protein binds the operator inhibiting transcription.

    • B. 

      In the absence of allolactose, the protein binds the operator inhibiting transcription.

    • C. 

      In the presence of allolactose, the protein binds the operator enhancing transcription.

    • D. 

      In the absence of allolactose, the protein binds the operator enhancing transcription.

    • E. 

      None of the rest.


  • 14. 
    Transcriptionally active DNA in eukaryotes is contained in ____.
    • A. 

      Euchromatin

    • B. 

      Heterochromatin

    • C. 

      Cytosol

    • D. 

      Endoplasmic reticulum

    • E. 

      Liposome


  • 15. 
    Eukaryotic promoter regions can contain canonical sequences, which mark the site of transcription initiation. One such sequence is the TATA box. Transcription in eukaryotes is mediated by the TATA box in the following way:
    • A. 

      RNA polymerase uses its sigma factor to bind to the TATA box and initiate transcription.

    • B. 

      DNA polymerase uses its alpha-2 factor to bind to the TATA box and recruits RNA polymerase to initiate transcription.

    • C. 

      The TATA box is not found in eukaryotes and is found only in prokaryotic promoter regions.

    • D. 

      The TATA-binding protein (TBP), a subunit of TFIIB binds the TATA box and aids in transcription initiation.

    • E. 

      The TATA-binding protein (TBP), a subunit of TFIID binds the TATA box and aids in transcription initiation.


  • 16. 
    You are a researcher studying a genetic disease, which results in neurodegeneration through production of an aberrant protein. To understand the impact this protein has within the cell, you decide to perform an experiment to reduce its expression in cells grown in a tissue culture. To do this, you:
    • A. 

      Use siRNA to target the mRNA transcript encoding the aberrant protein and stop its production.

    • B. 

      Add Neomycin, an aminoglycoside antibiotic that functions by halting protein synthesis within a cell, to your culture.

    • C. 

      Add lactose to your cells to change their metabolic profile by activating the lac operon.

    • D. 

      Use an inhibitor of cellular DNA polymerase to halt cellular transcription.

    • E. 

      You cannot reduce expression of a protein within a cell.


  • 17. 
    Prokaryotic mRNA encodes ____.
    • A. 

      A single protein because it is monocistronic.

    • B. 

      A single protein because it is polycistronic.

    • C. 

      Multiple proteins because it is monocistronic.

    • D. 

      Multiple proteins because it is polycistronic.

    • E. 

      DNA.


  • 18. 
    Which of the following statements regarding eukaryotic transcription is TRUE?
    • A. 

      It makes use of an RNA polymerase enzyme, which seeks out and binds promoters using its sigma factor.

    • B. 

      It produces a polycistronic mRNA allowing transcription and proteins synthesis to occur simultaneously.

    • C. 

      It is carried out in the cytosol by components of the RISC complex and the Dicer protein.

    • D. 

      It involves transcription factors, which facilitate transcription through protein-DNA and protein-protein interactions.

    • E. 

      It is coupled to translation.


  • 19. 
    ____ is a process essential to maturation of eukaryotic mRNA, and allows for production of multiple proteins from one gene.
    • A. 

      Transcription

    • B. 

      Splicing

    • C. 

      Translation

    • D. 

      Replication

    • E. 

      Transpeptidation


  • 20. 
    RNA transcription is apparently carried out by immobile protein complexes that reel in the ____.
    • A. 

      MRNA

    • B. 

      TRNA

    • C. 

      NcRNA

    • D. 

      RNA

    • E. 

      DNA


  • 21. 
    Chromatin remodeling may precede transcription. Which of the following is NOT a way that transcriptionally inactive DNA maintains a condensed state?
    • A. 

      Degree of DNA methylation

    • B. 

      Presence of variant histones

    • C. 

      Methylation of the promoters

    • D. 

      Pattern of histone modification

    • E. 

      The histone code


  • 22. 
    Which of the following is NOT one of the six highly conserved proteins that initiate transcription in eukaryotes?
    • A. 

      TFIIA

    • B. 

      TFIIB

    • C. 

      TFIID

    • D. 

      TFIIG

    • E. 

      TFIIF


  • 23. 
    During transcription, the DNA strands separate, and RNA polymerase constructs an RNA molecule that forms a short hybrid helix with the ____.
    • A. 

      DNA

    • B. 

      RNA polymerase

    • C. 

      MRNA

    • D. 

      Template strand

    • E. 

      NcRNA


  • 24. 
    When an activator binds to the enhancer, a protein complex known as Mediator links the enhancer-bound activator to the transcription machinery poised at the ____.
    • A. 

      Exon

    • B. 

      Intron

    • C. 

      Telomere

    • D. 

      Promoter

    • E. 

      RNA polymerase


  • 25. 
    The shift from initiation to elongation in eukaryotes involves structural changes in RNA polymerase, including ____ of its C-terminal domain
    • A. 

      Acetylation

    • B. 

      Methylation

    • C. 

      Phosphorylation

    • D. 

      Amidation

    • E. 

      Formylation


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