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Microbio 509

88 Questions
Microbio 509

Questions are taken from Carmen, microbiologyplace. Com (P) (pre-tests chap 1 and 2,

Questions and Answers
  • 1. 
    The bacterium Autumium quarterii has an inner membrane and a thick PG layer. After A. quarterii is Gram stained, how would you expect it to appear under the oil immersion lens?
    • A. 

      Purple

    • B. 

      Pink

    • C. 

      Mixture of purple and pink

    • D. 

      Bacillus shaped

  • 2. 
    Which one of the following statements is TRUE concerning hyphae?
    • A. 

      Hyphal morphology is found only in fungi.

    • B. 

      Streptomyces bacteria have hyphal morphology.

    • C. 

      Chains of bacillus bacteria may be described as hyphal.

    • D. 

      Yeast is commonly observed as hyphal upon microscopic observation.

  • 3. 
    You observe a sample of living bacteria under the microscope in lab and notice that they all seem to be moving in the same direction. This movement could best be described as,
    • A. 

      Motility

    • B. 

      Directed movement

    • C. 

      Brownian motion

    • D. 

      Current flow

  • 4. 
    Which one of the following is used to calculate the resolving power of the lens of a compound light microscope?
    • A. 

      Magnification of the lens

    • B. 

      The numerical aperture of the lens

    • C. 

      How bright the light is

    • D. 

      The working distance of the lens

  • 5. 
    A 3-phase streak was done in lab to,
    • A. 

      Dilute the culture so isolated colonies could be obtained

    • B. 

      Enumerate bacteria

    • C. 

      Serially dilute bacteria

    • D. 

      Identify certain pathogenic bacteria

  • 6. 
    Circle TWO of the following lab rules that are examples of using aseptic technique.
    • A. 

      Working on the lab top near your burner flame

    • B. 

      Cleaning your bench with Roccal (Cidecon) at the end of lab

    • C. 

      Returning materials to the supply cabinet at the end of lab

    • D. 

      Using lens cleaner and gauze to clean your microscope

    • E. 

      Keeping long hair tied back when in lab

  • 7. 
    What is one way you can visually tell if a live organism you are observing in a wet mount of ahay infusion is prokaryotic or eukaryotic?
    • A. 

      Eukaryotic organisms are usually much larger than prokaryotic ones

    • B. 

      Eukaryotic organisms are motile and prokaryotic ones are not

    • C. 

      Prokaryotic organisms have cell walls of peptidoglycan and eukaryotic do not

    • D. 

      Prokaryotic organisms are not visible under a light microscope

  • 8. 
    Which one of the following microscopes should you use to observe bacteria that have been Gram stained?
    • A. 

      Brightfield microscope

    • B. 

      Darkfield microscope

    • C. 

      Phase contrast microscope

    • D. 

      Fluorescence microscope

    • E. 

      Either the SEM or the TEM

  • 9. 
    Circle the correct answers in the statement below:Most of the dyes used to stain bacteria are (basic or acidic) since most bacteria have an overall (positive, negative) charge.
    • A. 

      Basic, negative

    • B. 

      Basic, positive

    • C. 

      Acidic, negative

    • D. 

      Acidic, positive

  • 10. 
    Where should you dispose of each of the following in lab: (NOTE: If biohazard container is partof any of your answers, be sure to describe as either the one on your lab bench or the one on the cart.) Gauze used to clean your microscope?
  • 11. 
    Where should you dispose of each of the following in lab: (NOTE: If biohazard container is partof any of your answers, be sure to describe as either the one on your lab bench or the one on the cart.) Culture in a Petri dish?
  • 12. 
    One objective lens on the compound light microscope MUST always be used with immersion oil. It is also the ONLY one that can be used with immersion oil. The magnification of this objective lens is
    • A. 

      10X

    • B. 

      40X

    • C. 

      100X

    • D. 

      1000X

  • 13. 
    Which one of the following slides will you view using the 100X oil immersion lens? (Lab 1)
    • A. 

      Dry mount of magazine photo

    • B. 

      Prepared slide of bacteria

    • C. 

      Wet mount of hay infusion

    • D. 

      All of the above

  • 14. 
    Where should you dispose of gauze used to clean immersion oil off of your microscope?
    • A. 

      Regular trash can

    • B. 

      Red bio-hazard bucket on lab bench

    • C. 

      White bio-hazard bucket on cart

    • D. 

      Take it home with you

  • 15. 
    Before you return your microscope to its storage cabinet, what should you have your lab instructor do?
    • A. 

      Clean the scope

    • B. 

      Collect any slides you prepared during the lab

    • C. 

      Inspect your drawings of bacteria

    • D. 

      Inspect the microscope

  • 16. 
    You will use a sterile swab to inoculate your plate during this exercise. Where should you dispose of the swab and the wrapper?
    • A. 

      Both in the regular trash

    • B. 

      Both in the red biohazard bucket

    • C. 

      Swab in the red biohazard bucket and wrapper in the regular trash

    • D. 

      Swab in the red biohazard bucket and wrapper in the white biohazard bucket

  • 17. 
    Which of the following is an advantage of an electron microscope over a light microscope?
    • A. 

      Electron scopes have higher resolution.

    • B. 

      Electron scopes are better for observation of motility.

    • C. 

      Electron scopes are easier to use in the lab.

    • D. 

      Electron scopes produce fluorescence in cells.

  • 18. 
    In which one of the following microscopes might light waves be added together to make endospores (or other cellular structures) appear very bright when viewed under the microscope?
    • A. 

      Brightfield microscope

    • B. 

      Dark field microscope

    • C. 

      Phase-contrast microscope

    • D. 

      Fluorescence microscope

  • 19. 
    Which of the following microbes that you will either use or observe this lab period are bacteria?
    • A. 

      Saccharomyces cerevisiae

    • B. 

      Proteus vulgaris

    • C. 

      Aspergillus niger

    • D. 

      Escherichia coli

  • 20. 
    Which of the following is TRUE concerning the yeast form of fungi?
    • A. 

      Yeast form interwoven mats known as mycelia.

    • B. 

      The yeast form is the most common form of fungi.

    • C. 

      Yeast forms grow as long branching filaments.

    • D. 

      The yeast forms of fungi are unicellular.

  • 21. 
    A student takes a sample from a colony growing on a plate and makes a wet mount. When she observes the sample using the 40X objective, she notes the presence of buds. The microbes in the colony are likely
    • A. 

      Bacteria since this is a common form of reproduction for bacteria.

    • B. 

      Hyphal fungi since hyphal fungi form buds when they grow on plates.

    • C. 

      Yeast since they commonly reproduce asexually by budding.

    • D. 

      Tiny plants just ready to produce flowers.

  • 22. 
    If you look at a sample from hay infusion and see very small microbes moving but no cilia or flagella are visible, the microbes are most likely
    • A. 

      Prokaryotic

    • B. 

      Eukaryotic

    • C. 

      Yeast

    • D. 

      Fungi

  • 23. 
    When taking a sample from a broth culture, why should you vortex the tube?
    • A. 

      To mix oxygen into the culture

    • B. 

      To resuspend the organisms

    • C. 

      To separate the organisms from the broth

    • D. 

      To sterilize the tube

  • 24. 
    When you flame sterilize your inoculating loop, you must hold the inoculating loop in the flame until which of the following glows red hot?
    • A. 

      Just the round loop at the end

    • B. 

      The entire length of the wire including the round loop

    • C. 

      The handle

    • D. 

      No part should be red-hot

  • 25. 
    Which of the following are LIGHT microscopes?
    • A. 

      Brightfield

    • B. 

      Darkfield

    • C. 

      SEM

    • D. 

      TEM

    • E. 

      Fluorsecent

  • 26. 
    When comparing the images produced by the SEM and the TEM, the image produced by the SEM
    • A. 

      Gives more of an internal view of the specimen

    • B. 

      Is more two-dimensional

    • C. 

      Shows the surface of the specimen

    • D. 

      Shows dark spots produced by pairs of antibodies

  • 27. 
    Which of the following microbes are eukaryotic?
    • A. 

      Saccharomyces Cerevisiae

    • B. 

      Proteus Vulgaris

    • C. 

      Aspergillus Niger

    • D. 

      Escherichia Coli

  • 28. 
    Most fungi are described as hyphal. Hyphae form mats of growth known as
    • A. 

      Spores

    • B. 

      Mycelia

    • C. 

      Yeast

    • D. 

      Colonies

  • 29. 
    Which of the following would be a way to microscopically distinguish bacteria from yeast?
    • A. 

      Yeast cells should be much larger than bacterial cells

    • B. 

      Yeast should appear as "fuzzy" hyphae on the plate

    • C. 

      Nuclei should be visible in bacteria but not in yeast

    • D. 

      Yeast cells will have flagella

  • 30. 
    If you look at a sample from hay infusion and see microbes moving with visible cilia or flagella, the microbes must be
    • A. 

      Prokaryotic

    • B. 

      Eukaryotic

    • C. 

      Yeast

    • D. 

      Fungi

  • 31. 
    When making a wet mount from a broth culture, how should you remove the cap of tube of culture?
    • A. 

      Remove the cap and lay it open side down on a clean area of your lab bench.

    • B. 

      Remove the cap with the little finger of the hand you use to hold the loop.

    • C. 

      Remove the cap with the opposite hand in which you hold the loop.

    • D. 

      Remove the cap and dispose of it in the red biohazard bucket on your bench.

  • 32. 
    When you flame sterilize your inoculating loop, you must hold the inoculating loop in the flame until which of the following glows red hot?
    • A. 

      Just the round loop at the end

    • B. 

      The entire length of the wire including the round loop

    • C. 

      The handle

    • D. 

      No part should be red-hot

  • 33. 
    The first observations of bacteria were made by ________. P
    • A. 

      Needham

    • B. 

      Pasteur

    • C. 

      Van Leeuwenhoek

    • D. 

      Hooke

  • 34. 
    All of the following scientists performed experiments that supported the theory of biogenesis except ________. P
    • A. 

      Needham

    • B. 

      Virchow

    • C. 

      Redi

    • D. 

      Pasteur

  • 35. 
    Working with the French wine industry, Louis Pasteur developed a process that ________. P
    • A. 

      Sterilizes the wine

    • B. 

      Removes spoilage microorganisms from wine

    • C. 

      Improves the nutritional value of wine

    • D. 

      Promotes the growth of yeast during the wine-making process

  • 36. 
    West Nile Virus is transmitted to humans by _______. P
    • A. 

      Contaminated food

    • B. 

      Mosquitos

    • C. 

      Fleas

    • D. 

      Ticks

  • 37. 
    All of the following are products produced naturally by microorganisms except which one? P
    • A. 

      Butyl alcohol

    • B. 

      Silk

    • C. 

      Acetone

    • D. 

      Ethyl alcohol

  • 38. 
    Which of the following scientists and their discoveries is not correctly matched? P
    • A. 

      Lister; antibiotics

    • B. 

      Koch; germ theory of disease

    • C. 

      Ehrlich; chemotherapy

    • D. 

      Jenner; vaccination

  • 39. 
    Which of the following is a scientific name? P
    • A. 

      Legionnaires' bacterium

    • B. 

      Philadelphia fever

    • C. 

      Legionella pneumophila

    • D. 

      Legionnaires'' disease

  • 40. 
    Which of the following fields of study and its example are not correctly matched? P
    • A. 

      Parasitology; study of HIV

    • B. 

      Immunology; study of vaccines

    • C. 

      Bacteriology; study of E. Coli 0157:H7

    • D. 

      Mycology; study of athlete's foot

  • 41. 
    In the scientific name, Escherichia coli, Escherichia is the _______. P
    • A. 

      Family

    • B. 

      Species

    • C. 

      Genus

    • D. 

      Class

  • 42. 
    Which of the following types of microorganism and its description is not correctly matched? P
    • A. 

      Protozoa; small animals

    • B. 

      Helminthes; multicellular animals

    • C. 

      Fungi; eukaryotic

    • D. 

      Bacteria; prokaryotic

  • 43. 
    Which of the following is not a characteristic of Bacteria? P
    • A. 

      All are pathogenic

    • B. 

      The reproduce by a process called binary fission

    • C. 

      All are unicellular

    • D. 

      Cell walls are composed of peptidoglycan

  • 44. 
    Which of the following does not belong with the others? P
    • A. 

      Animals

    • B. 

      Fungi

    • C. 

      Plants

    • D. 

      Bacteria

  • 45. 
    The laboratory and medical procedures employed to prevent microbial contamination are called ________. P
    • A. 

      Koch's postulates

    • B. 

      Sterilization

    • C. 

      Aseptic techniques

    • D. 

      Bioremediation

  • 46. 
    All of the following are examples of biotechnology except ______. P
    • A. 

      Bacteria that cause disease

    • B. 

      Enzyme production by bacteria and fungi

    • C. 

      Antibiotic production by bacteria

    • D. 

      Bread making with bacteria and fungi

  • 47. 
    Avian influenza has not yet become widespread among humans because ________. P
    • A. 

      Humans are vaccinated against avian influenza

    • B. 

      It is not readily transmitted from human to human

    • C. 

      It infects only birds

    • D. 

      When humans are infected, they do not get sick

  • 48. 
    Emerging infectious diseases can be due to ________. P
    • A. 

      Deforestation and earthquake

    • B. 

      Changes or mutations in existing organisms

    • C. 

      Humans moving to previously uninhabited areas

    • D. 

      All of the above

  • 49. 
    Which one of the following is an example of bioremediation? P
    • A. 

      Use of Sacccharomyces cerevisiae to make wine

    • B. 

      Use of Pseudomonas to remove uranium from soil at weapons depots

    • C. 

      Use of Bacillus thuringiensis to kill crop-eating insects

    • D. 

      Use of cowpox virus to vaccinate against smallpox

  • 50. 
    Which of the following processes are beneficial activities of microorganisms? P
    • A. 

      Recycling elements in the environment

    • B. 

      Decomposing sewage

    • C. 

      Producing foods and chemicals

    • D. 

      All of the above

  • 51. 
    Which of the following best describes the introduction of a human gene into bacteria to induce the bacteria to produce the human protein? P
    • A. 

      Recombinant DNA technology

    • B. 

      Bioremediation

    • C. 

      Gene therapy

    • D. 

      Genomics

  • 52. 
    Which of the following statements about bacteria is not true? P
    • A. 

      Bacteria are essential to the presence of life on Earth

    • B. 

      Bacteria are used to make some foods

    • C. 

      The only good bacterium is a dead bacterium

    • D. 

      Everyone has bacteria in and on the body

  • 53. 
    The type of bond holding Na+ and Cl- ions in NaCl is a/an ________. P
    • A. 

      Covalent bond

    • B. 

      Hydrogen bond

    • C. 

      Double bond

    • D. 

      Ionic bond

  • 54. 
    The type of bond holding C and H atoms in glucose is a/an ________. P
    • A. 

      Hydrogen bond

    • B. 

      Double bond

    • C. 

      Ionic bond

    • D. 

      Covalent bond

  • 55. 
    Which of the following chemical bonds is the strongest? P
    • A. 

      Hydrophobic bonds

    • B. 

      Ionic bonds

    • C. 

      Hydrogen bonds

    • D. 

      Covalent bonds

  • 56. 
    Which of the following molecules contain long hydrocarbon chains? P
    • A. 

      Lipids

    • B. 

      Nucleic acids

    • C. 

      Proteins

    • D. 

      Carbohydrates

  • 57. 
    Which of following molecules contains amino groups? P
    • A. 

      Nucleic acids

    • B. 

      Proteins

    • C. 

      Lipids

    • D. 

      Carbohydrates

  • 58. 
    Ribose and adenine can be found in RNA and in _______. P
    • A. 

      Carbohydrates

    • B. 

      ATP

    • C. 

      DNA

    • D. 

      Proteins

  • 59. 
    Which of the following statements about the atom 12C is not true? P
    • A. 

      Its atomic weight is 12

    • B. 

      It has 6 protons in its nucleus

    • C. 

      It has 12 neutrons in its nucleus

    • D. 

      Its atomic number is 6

  • 60. 
    Which of the following statements about the atom Na (atomic number 11) is not true? P
    • A. 

      It has the same number if protons and electrons

    • B. 

      It has 11 electrons

    • C. 

      It has 11 protons

    • D. 

      It has the same number of protons and neutrons

  • 61. 
    Water is an excellent solvent as well as an effective temperature buffer. This is due to ________. PP
    • A. 

      The ionic bonds in water

    • B. 

      The pH of water

    • C. 

      The polar nature of water

    • D. 

      The pH buffering capacity of water

  • 62. 
    How does the hydrogen ion concentration in lemon juice (pH 2) compare to the hydrogen ion concentration in tomato juice (pH 4)? P
    • A. 

      Lemon juice has a hundredfold higher hydrogen ion concentration than tomato juice.

    • B. 

      Lemon juice has a hundredfold lower hydrogen ion concentration than tomato juice.

    • C. 

      Lemon juice has a twofold lower hydrogen ion concentration than tomato juice.

    • D. 

      Lemon juice has a twofold higher hydrogen ion concentration than tomato juice.

  • 63. 
    Phosphorous is found in all of the following cell components except ________. P
    • A. 

      Proteins

    • B. 

      RNA

    • C. 

      Plasma membrane

    • D. 

      ATP

  • 64. 
    Nitrogen is found in all of the following cell components except ________. P
    • A. 

      Carbohydrates

    • B. 

      ATP

    • C. 

      RNA

    • D. 

      Proteins

  • 65. 
    Detergents can combine with lipid molecules. Which part of a cell would you expect detergents to damage? P
    • A. 

      Proteins

    • B. 

      Cell wall

    • C. 

      Nucleus

    • D. 

      Plasma membrane

  • 66. 
    Which of the compounds listed below is an inorganic compound? P
    • A. 

      Glucose

    • B. 

      DNA

    • C. 

      Proteins

    • D. 

      NaCl

  • 67. 
    Which of the following is an example of anabolism? P
    • A. 

      Converting DNA to nucleotides

    • B. 

      Forming cellulose from glucose

    • C. 

      Converting a protein to amino acids

    • D. 

      Converting glucose to CO2 and H2O during cell respiration

  • 68. 
    Which of the following statements about cellulase is true? P
    • A. 

      It is one of the most abundant carbohydrates on earth

    • B. 

      It is made by some bacteria and fungi

    • C. 

      It is one of the digestive enzymes that we make to digest cellulose

    • D. 

      It is found in a plant cell walls

  • 69. 
    12C and 14C atoms differ in the ________. P
    • A. 

      Number of protons in the nucleus

    • B. 

      Ability of cells to use them

    • C. 

      Number of neutrons in the nucleus

    • D. 

      Number of electrons in the shells

  • 70. 
    What kind of chemical reaction is involved when bacteria are used for the bioremediation of a petroleum spill? P
    • A. 

      Dehydration reaction

    • B. 

      Synthesis reaction

    • C. 

      Decomposition reaction

    • D. 

      Exchange reaction

  • 71. 
    Which of the following terms does no belong with the others? P
    • A. 

      Glycerol

    • B. 

      Amino group

    • C. 

      Fatty acid

    • D. 

      Saturated

  • 72. 
    Which of the following terms does not belong with the others? P
    • A. 

      Nitrogenous base

    • B. 

      Deoxyribose

    • C. 

      Amino group

    • D. 

      Phosphate group

  • 73. 
    The chemical reactions involved in protein synthesis and synthesis of cell wall peptidoglycan are examples of_________ .
    • A. 

      Catabolic reactions

    • B. 

      Autotrophic reactions

    • C. 

      Anabolic reactions

    • D. 

      Photosynthetic reactions

  • 74. 
    What is the cellular arrangement?OOOOOO
    • A. 

      Strepto

    • B. 

      Diplo

    • C. 

      Basillo

    • D. 

      Staphylo

  • 75. 
    Ex. 6, Per. 2: Which one of the following mistakes might cause you to find more than one type of colony on your streak plate?
    • A. 

      You overlapped streaks in the sector.

    • B. 

      You did not vortex the original culture.

    • C. 

      You did not use good aseptic technique.

    • D. 

      You dug the loop into the agar.

  • 76. 
    Ex. 6, Per. 2:  The following suggestion will help you improve your spread plate technique:  When  making a spread plate, do not resuspend your culture.
    • A. 

      True

    • B. 

      False

  • 77. 
    Ex. 7: In the acid-fast stain, the decolorizning agent(s) is (are)
    • A. 

      Hydrochloric acid and alcohol.

    • B. 

      Methylene blue.

    • C. 

      Turgitol and phenol.

    • D. 

      Carbolfuchsin.

    • E. 

      Crystal violet.

  • 78. 
    Ex. 8: The smear of Bacillus megaterium must be heat fixed before using the capsule stain.
    • A. 

      True

    • B. 

      False

  • 79. 
    Ex. 9: Endospores are reproductive cells formed by some bacteria.
    • A. 

      True

    • B. 

      False

  • 80. 
    Stains (Ex. 7, 8, 9):  Look at the three photomicrographs above. Which one of the following micrographs represents a sample stained using the Capsule stain?
    • A. 

      Slide A

    • B. 

      Slide B

    • C. 

      Slide C

  • 81. 
    Ex. 10: The most common method used to separate mixed cultures is the
    • A. 

      Serial dliution

    • B. 

      Gram-stain

    • C. 

      Streak plate method

    • D. 

      Spread plate method

  • 82. 
    Ex.6, Per.2: Which of the following mistakes might cause the growth in all three of the sectors on the streak plate to look the same?
    • A. 

      You didn't flame between sectors.

    • B. 

      You didn't vortex your culture.

    • C. 

      You dug into the agar with your loop.

    • D. 

      You overlapped the lines in your streak.

  • 83. 
    Ex. 6, Per. 2: The following suggestion should help you improve your spread plate technique: When making a spread plate for a lawn of growth, you should streak the plate 3 times in 3 different directions.
    • A. 

      True

    • B. 

      False

  • 84. 
    Ex. 7: Bacteria with a waxy layer of mycolic acid
    • A. 

      Cannot be successfully Gram stained.

    • B. 

      Cannot be differentially stained.

    • C. 

      Cannot be heat fixed to a slide.

    • D. 

      Cannot be grown on a medium in lab.

  • 85. 
    Ex. 8: A capsule stain is described as a negative stain because
    • A. 

      It gives no information about cell wall type.

    • B. 

      The capsule does not take up the stain.

    • C. 

      The capsule has an overall negative charge.

    • D. 

      Cells with capsules are gram-negative.

  • 86. 
    Ex. 8: After completing the capsule stain, the slides must be air dried rather than blotting with bibulous paper.
    • A. 

      True

    • B. 

      False

  • 87. 
    Ex. 9: Bacteria produce endospores for both reproduction and survival.Stains
    • A. 

      True

    • B. 

      False

  • 88. 
    (Ex. 7, 8, 9): Look at the three photomicrographs above. Which one of the following micrographs represents a sample stained using the Endospore stain?
    • A. 

      Slide A

    • B. 

      Slide B

    • C. 

      Slide C

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