Microbio 509
Questions are taken from Carmen, microbiologyplace. Com (P) (pre-tests chap 1 and 2,
- 1.
The bacterium Autumium quarterii has an inner membrane and a thick PG layer. After A. quarterii is Gram stained, how would you expect it to appear under the oil immersion lens?
- A.
Purple
- B.
Pink
- C.
Mixture of purple and pink
- D.
Bacillus shaped
Correct Answer
A. Purple -
- 2.
Which one of the following statements is TRUE concerning hyphae?
- A.
Hyphal morphology is found only in fungi.
- B.
Streptomyces bacteria have hyphal morphology.
- C.
Chains of bacillus bacteria may be described as hyphal.
- D.
Yeast is commonly observed as hyphal upon microscopic observation.
Correct Answer
B. Streptomyces bacteria have hyphal morphology. -
- 3.
You observe a sample of living bacteria under the microscope in lab and notice that they all seem to be moving in the same direction. This movement could best be described as,
- A.
Motility
- B.
Directed movement
- C.
Brownian motion
- D.
Current flow
Correct Answer
D. Current flow -
- 4.
Which one of the following is used to calculate the resolving power of the lens of a compound light microscope?
- A.
Magnification of the lens
- B.
The numerical aperture of the lens
- C.
How bright the light is
- D.
The working distance of the lens
Correct Answer
B. The numerical aperture of the lens -
- 5.
A 3-phase streak was done in lab to,
- A.
Dilute the culture so isolated colonies could be obtained
- B.
Enumerate bacteria
- C.
Serially dilute bacteria
- D.
Identify certain pathogenic bacteria
Correct Answer
A. Dilute the culture so isolated colonies could be obtained -
- 6.
Circle TWO of the following lab rules that are examples of using aseptic technique.
- A.
Working on the lab top near your burner flame
- B.
Cleaning your bench with Roccal (Cidecon) at the end of lab
- C.
Returning materials to the supply cabinet at the end of lab
- D.
Using lens cleaner and gauze to clean your microscope
- E.
Keeping long hair tied back when in lab
Correct Answer(s)
A. Working on the lab top near your burner flame
B. Cleaning your bench with Roccal (Cidecon) at the end of lab -
- 7.
What is one way you can visually tell if a live organism you are observing in a wet mount of ahay infusion is prokaryotic or eukaryotic?
- A.
Eukaryotic organisms are usually much larger than prokaryotic ones
- B.
Eukaryotic organisms are motile and prokaryotic ones are not
- C.
Prokaryotic organisms have cell walls of peptidoglycan and eukaryotic do not
- D.
Prokaryotic organisms are not visible under a light microscope
Correct Answer
A. Eukaryotic organisms are usually much larger than prokaryotic ones -
- 8.
Which one of the following microscopes should you use to observe bacteria that have been Gram stained?
- A.
Brightfield microscope
- B.
Darkfield microscope
- C.
Phase contrast microscope
- D.
Fluorescence microscope
- E.
Either the SEM or the TEM
Correct Answer
A. Brightfield microscope -
- 9.
Circle the correct answers in the statement below:Most of the dyes used to stain bacteria are (basic or acidic) since most bacteria have an overall (positive, negative) charge.
- A.
Basic, negative
- B.
Basic, positive
- C.
Acidic, negative
- D.
Acidic, positive
Correct Answer
A. Basic, negative -
- 10.
Where should you dispose of each of the following in lab: (NOTE: If biohazard container is partof any of your answers, be sure to describe as either the one on your lab bench or the one on the cart.) Gauze used to clean your microscope?
Correct Answer
put in regular trash
trash
regular trash - 11.
Where should you dispose of each of the following in lab: (NOTE: If biohazard container is partof any of your answers, be sure to describe as either the one on your lab bench or the one on the cart.) Culture in a Petri dish?
Correct Answer
large biohazard on cart - 12.
One objective lens on the compound light microscope MUST always be used with immersion oil. It is also the ONLY one that can be used with immersion oil. The magnification of this objective lens is
- A.
10X
- B.
40X
- C.
100X
- D.
1000X
Correct Answer
C. 100X -
- 13.
Which one of the following slides will you view using the 100X oil immersion lens? (Lab 1)
- A.
Dry mount of magazine photo
- B.
Prepared slide of bacteria
- C.
Wet mount of hay infusion
- D.
All of the above
Correct Answer
B. Prepared slide of bacteria -
- 14.
Where should you dispose of gauze used to clean immersion oil off of your microscope?
- A.
Regular trash can
- B.
Red bio-hazard bucket on lab bench
- C.
White bio-hazard bucket on cart
- D.
Take it home with you
Correct Answer
A. Regular trash can -
- 15.
Before you return your microscope to its storage cabinet, what should you have your lab instructor do?
- A.
Clean the scope
- B.
Collect any slides you prepared during the lab
- C.
Inspect your drawings of bacteria
- D.
Inspect the microscope
Correct Answer
D. Inspect the microscope -
- 16.
You will use a sterile swab to inoculate your plate during this exercise. Where should you dispose of the swab and the wrapper?
- A.
Both in the regular trash
- B.
Both in the red biohazard bucket
- C.
Swab in the red biohazard bucket and wrapper in the regular trash
- D.
Swab in the red biohazard bucket and wrapper in the white biohazard bucket
Correct Answer
C. Swab in the red biohazard bucket and wrapper in the regular trash -
- 17.
Which of the following is an advantage of an electron microscope over a light microscope?
- A.
Electron scopes have higher resolution.
- B.
Electron scopes are better for observation of motility.
- C.
Electron scopes are easier to use in the lab.
- D.
Electron scopes produce fluorescence in cells.
Correct Answer
A. Electron scopes have higher resolution. -
- 18.
In which one of the following microscopes might light waves be added together to make endospores (or other cellular structures) appear very bright when viewed under the microscope?
- A.
Brightfield microscope
- B.
Dark field microscope
- C.
Phase-contrast microscope
- D.
Fluorescence microscope
Correct Answer
C. Phase-contrast microscope -
- 19.
Which of the following microbes that you will either use or observe this lab period are bacteria?
- A.
Saccharomyces cerevisiae
- B.
Proteus vulgaris
- C.
Aspergillus niger
- D.
Escherichia coli
Correct Answer(s)
B. Proteus vulgaris
D. Escherichia coli -
- 20.
Which of the following is TRUE concerning the yeast form of fungi?
- A.
Yeast form interwoven mats known as mycelia.
- B.
The yeast form is the most common form of fungi.
- C.
Yeast forms grow as long branching filaments.
- D.
The yeast forms of fungi are unicellular.
Correct Answer
D. The yeast forms of fungi are unicellular. -
- 21.
A student takes a sample from a colony growing on a plate and makes a wet mount. When she observes the sample using the 40X objective, she notes the presence of buds. The microbes in the colony are likely
- A.
Bacteria since this is a common form of reproduction for bacteria.
- B.
Hyphal fungi since hyphal fungi form buds when they grow on plates.
- C.
Yeast since they commonly reproduce asexually by budding.
- D.
Tiny plants just ready to produce flowers.
Correct Answer
C. Yeast since they commonly reproduce asexually by budding. -
- 22.
If you look at a sample from hay infusion and see very small microbes moving but no cilia or flagella are visible, the microbes are most likely
- A.
Prokaryotic
- B.
Eukaryotic
- C.
Yeast
- D.
Fungi
Correct Answer
A. Prokaryotic -
- 23.
When taking a sample from a broth culture, why should you vortex the tube?
- A.
To mix oxygen into the culture
- B.
To resuspend the organisms
- C.
To separate the organisms from the broth
- D.
To sterilize the tube
Correct Answer
B. To resuspend the organisms -
- 24.
When you flame sterilize your inoculating loop, you must hold the inoculating loop in the flame until which of the following glows red hot?
- A.
Just the round loop at the end
- B.
The entire length of the wire including the round loop
- C.
The handle
- D.
No part should be red-hot
Correct Answer
B. The entire length of the wire including the round loop -
- 25.
Which of the following are LIGHT microscopes?
- A.
Brightfield
- B.
Darkfield
- C.
SEM
- D.
TEM
- E.
Fluorsecent
Correct Answer(s)
A. Brightfield
B. Darkfield
E. Fluorsecent -
- 26.
When comparing the images produced by the SEM and the TEM, the image produced by the SEM
- A.
Gives more of an internal view of the specimen
- B.
Is more two-dimensional
- C.
Shows the surface of the specimen
- D.
Shows dark spots produced by pairs of antibodies
Correct Answer
C. Shows the surface of the specimen -
- 27.
Which of the following microbes are eukaryotic?
- A.
Saccharomyces Cerevisiae
- B.
Proteus Vulgaris
- C.
Aspergillus Niger
- D.
Escherichia Coli
Correct Answer(s)
A. Saccharomyces Cerevisiae
C. Aspergillus Niger -
- 28.
Most fungi are described as hyphal. Hyphae form mats of growth known as
- A.
Spores
- B.
Mycelia
- C.
Yeast
- D.
Colonies
Correct Answer
B. Mycelia -
- 29.
Which of the following would be a way to microscopically distinguish bacteria from yeast?
- A.
Yeast cells should be much larger than bacterial cells
- B.
Yeast should appear as "fuzzy" hyphae on the plate
- C.
Nuclei should be visible in bacteria but not in yeast
- D.
Yeast cells will have flagella
Correct Answer
A. Yeast cells should be much larger than bacterial cells -
- 30.
If you look at a sample from hay infusion and see microbes moving with visible cilia or flagella, the microbes must be
- A.
Prokaryotic
- B.
Eukaryotic
- C.
Yeast
- D.
Fungi
Correct Answer
B. Eukaryotic -
- 31.
When making a wet mount from a broth culture, how should you remove the cap of tube of culture?
- A.
Remove the cap and lay it open side down on a clean area of your lab bench.
- B.
Remove the cap with the little finger of the hand you use to hold the loop.
- C.
Remove the cap with the opposite hand in which you hold the loop.
- D.
Remove the cap and dispose of it in the red biohazard bucket on your bench.
Correct Answer
B. Remove the cap with the little finger of the hand you use to hold the loop. -
- 32.
When you flame sterilize your inoculating loop, you must hold the inoculating loop in the flame until which of the following glows red hot?
- A.
Just the round loop at the end
- B.
The entire length of the wire including the round loop
- C.
The handle
- D.
No part should be red-hot
Correct Answer
B. The entire length of the wire including the round loop -
- 33.
The first observations of bacteria were made by ________. P
- A.
Needham
- B.
Pasteur
- C.
Van Leeuwenhoek
- D.
Hooke
Correct Answer
C. Van Leeuwenhoek -
- 34.
All of the following scientists performed experiments that supported the theory of biogenesis except ________. P
- A.
Needham
- B.
Virchow
- C.
Redi
- D.
Pasteur
Correct Answer
A. Needham -
- 35.
Working with the French wine industry, Louis Pasteur developed a process that ________. P
- A.
Sterilizes the wine
- B.
Removes spoilage microorganisms from wine
- C.
Improves the nutritional value of wine
- D.
Promotes the growth of yeast during the wine-making process
Correct Answer
B. Removes spoilage microorganisms from wine -
- 36.
West Nile Virus is transmitted to humans by _______. P
- A.
Contaminated food
- B.
Mosquitos
- C.
Fleas
- D.
Ticks
Correct Answer
B. Mosquitos -
- 37.
All of the following are products produced naturally by microorganisms except which one? P
- A.
Butyl alcohol
- B.
Silk
- C.
Acetone
- D.
Ethyl alcohol
Correct Answer
B. Silk -
- 38.
Which of the following scientists and their discoveries is not correctly matched? P
- A.
Lister; antibiotics
- B.
Koch; germ theory of disease
- C.
Ehrlich; chemotherapy
- D.
Jenner; vaccination
Correct Answer
A. Lister; antibiotics -
- 39.
Which of the following is a scientific name? P
- A.
Legionnaires' bacterium
- B.
Philadelphia fever
- C.
Legionella pneumophila
- D.
Legionnaires'' disease
Correct Answer
C. Legionella pneumophila -
- 40.
Which of the following fields of study and its example are not correctly matched? P
- A.
Parasitology; study of HIV
- B.
Immunology; study of vaccines
- C.
Bacteriology; study of E. Coli 0157:H7
- D.
Mycology; study of athlete's foot
Correct Answer
A. Parasitology; study of HIV -
- 41.
In the scientific name, Escherichia coli, Escherichia is the _______. P
- A.
Family
- B.
Species
- C.
Genus
- D.
Class
Correct Answer
C. Genus -
- 42.
Which of the following types of microorganism and its description is not correctly matched? P
- A.
Protozoa; small animals
- B.
Helminthes; multicellular animals
- C.
Fungi; eukaryotic
- D.
Bacteria; prokaryotic
Correct Answer
A. Protozoa; small animals -
- 43.
Which of the following is not a characteristic of Bacteria? P
- A.
All are pathogenic
- B.
The reproduce by a process called binary fission
- C.
All are unicellular
- D.
Cell walls are composed of peptidoglycan
Correct Answer
A. All are pathogenic -
- 44.
Which of the following does not belong with the others? P
- A.
Animals
- B.
Fungi
- C.
Plants
- D.
Bacteria
Correct Answer
D. Bacteria -
- 45.
The laboratory and medical procedures employed to prevent microbial contamination are called ________. P
- A.
Koch's postulates
- B.
Sterilization
- C.
Aseptic techniques
- D.
Bioremediation
Correct Answer
C. Aseptic techniques -
- 46.
All of the following are examples of biotechnology except ______. P
- A.
Bacteria that cause disease
- B.
Enzyme production by bacteria and fungi
- C.
Antibiotic production by bacteria
- D.
Bread making with bacteria and fungi
Correct Answer
A. Bacteria that cause disease -
- 47.
Avian influenza has not yet become widespread among humans because ________. P
- A.
Humans are vaccinated against avian influenza
- B.
It is not readily transmitted from human to human
- C.
It infects only birds
- D.
When humans are infected, they do not get sick
Correct Answer
B. It is not readily transmitted from human to human -
- 48.
Emerging infectious diseases can be due to ________. P
- A.
Deforestation and earthquake
- B.
Changes or mutations in existing organisms
- C.
Humans moving to previously uninhabited areas
- D.
All of the above
Correct Answer
D. All of the above -
- 49.
Which one of the following is an example of bioremediation? P
- A.
Use of Sacccharomyces cerevisiae to make wine
- B.
Use of Pseudomonas to remove uranium from soil at weapons depots
- C.
Use of Bacillus thuringiensis to kill crop-eating insects
- D.
Use of cowpox virus to vaccinate against smallpox
Correct Answer
B. Use of Pseudomonas to remove uranium from soil at weapons depots -
- 50.
Which of the following processes are beneficial activities of microorganisms? P
- A.
Recycling elements in the environment
- B.
Decomposing sewage
- C.
Producing foods and chemicals
- D.
All of the above
Correct Answer
D. All of the above -
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