Biochemistry 3 (Tests)
- 1.
The component (s) of DNA which is responsible for the absorbanc band at 250-270 nm is
- A.
Bases, pentoses, and phosphates all contribute
- B.
Only bases
- C.
Only bases and pentoses
- D.
Only pentoses and phosphates
Correct Answer
B. Only bases -
- 2.
To what target sequence on DNA is the oligonucleotide pAATTCCGG complementary? (sequence are written as 5'-3')
- A.
TTAAGGCC
- B.
CCGGAATT
- C.
UUAAGGCC
- D.
CCGGAAUU
Correct Answer
B. CCGGAATT -
- 3.
In eukaryotic cells, the only RNA molecule which has a poly (A) tail attached to the 3' end is
- A.
MRNA
- B.
TRNA
- C.
SS RNA
- D.
All rRNA's
Correct Answer
A. MRNA -
- 4.
The RNA molecules which are involved in processing the hnRNA are
- A.
MRNA
- B.
TRNA
- C.
RRNA
- D.
SnRNA
Correct Answer
D. SnRNA -
- 5.
Partial digestion of the oligonucleotide d-AGTACAA by pancreatic phosphodiesterase (a "b" cutter) would result in which set of products?
- A.
AGP, pAC, pAA
- B.
AGPp, AC, pAA
- C.
AGp, TAp, CAA
- D.
AG, TAp, pCCA
Correct Answer
C. AGp, TAp, CAA -
- 6.
Which of the following is not a characteristic for DNA synthesis?
- A.
DNA polymerase adds nucleotides in a 5' --> 3' direction
- B.
A primer strand of DNA must contain a free 3'-OH end
- C.
The primer strand of DNA determines which nucleotides are added next
- D.
The correct complementary hydrogen bonding between base pairs is the primary check on the fidelity of the newly synthesized DNA
Correct Answer
C. The primer strand of DNA determines which nucleotides are added next -
- 7.
A DNA is found to exhibit a melting temperature of 75 degrees Celsius in 0.1M NaCl. Under what experimental conditions would the Tm be increased? (pg 372)
- A.
Decreasing the concentration of DNA
- B.
Increasing the concentration of NaCl in the solution
- C.
Adding 1% urea (an organic denaturant)
- D.
All of the above
Correct Answer
B. Increasing the concentration of NaCl in the solution -
- 8.
Which of the following characteristics is associated with the B form of DNA?
- A.
The sugars are located in the interior of the double helix
- B.
B
Correct Answer
A. The sugars are located in the interior of the double helix -
- 9.
A feature which is distinctive to Z-DNA is
- A.
A left-handed helix
- B.
it has 12 bases per turn
- C.
It is found in vivox
- D.
All of the above
Correct Answer
D. All of the above -
- 10.
How many helical turns (the linking number) are found in a relaxed circular DNA of 4500 bp?
- A.
500
- B.
50
- C.
450
- D.
350
Correct Answer
C. 450 -
- 11.
The linking number of a highly supercoiled plasmid can be readily changed without producing a relaxed DNA by
- A.
Changing the temperature
- B.
Increasing salt concentration
- C.
The action of topoisomerase
- D.
The action of a nuclease such as pancreatic DNAse
Correct Answer
C. The action of topoisomerase -
- 12.
Under which of the following conditions does the melting temperature for the denaturation of duplex DNA decrease?
- A.
Higher salt concentration in the buffer
- B.
Higher GC content in the DNA
- C.
PH higher than 10 in the buffer
- D.
Longer DNA fragments
Correct Answer
C. PH higher than 10 in the buffer -
- 13.
Which of the following double-stranded DNAs may convert to a cruciform structure?
- A.
ATGCATGCATGCATGC / TACGTACGTACGTACG
- B.
AAAAAAAAACCCCCCCCC / TTTTTTTTTGGGGGGGGC
- C.
ATATATATGCGCGCGCGC / TATATATACGCGCGCGCG
- D.
CATTAAGCAGTGCTTAAGA / GAATTCGTCACGAATTCT
Correct Answer
A. ATGCATGCATGCATGC / TACGTACGTACGTACG -
- 14.
Which of the following statements is incorrect when dsDNA is denatured? (pg 371-2)
- A.
The absorbance at 260 decreases about 40%
- B.
The hgiher the G+C content, the higher the melting temperature
- C.
The transition from dsDNA to ssDNA is highly cooperative
- D.
The fraction of DNA that is single stranded is 0.5 at its Tm
Correct Answer
A. The absorbance at 260 decreases about 40% -
- 15.
What is the primary role of the template strand in DNA replication?
- A.
It serves as the start point for the new DNA strand
- B.
It serves as a guide in determining the next nucleotide to be added by the polymerase
- C.
It increases the processivity of the DNA polymerase
- D.
It is essential for the 3-5' exonuclease activity
Correct Answer
B. It serves as a guide in determining the next nucleotide to be added by the polymerase -
- 16.
In the repair of DNA, the E.coli DNA polymerase invovled performs which function in addition to its polymerase activity?
- A.
It recognizes the abnormality in the duplex DNA structure.
- B.
It nicks the DNA to provide a 3'-OH
- C.
It removes the damaged section of DNA with its 5'-3' exonuclease activity
- D.
It ligates the DNA after the repair synthesis
Correct Answer
C. It removes the damaged section of DNA with its 5'-3' exonuclease activity -
- 17.
Why is a primer strand needed in DNA replication?
- A.
It insures the fidelity of the newly synthesized DNA strand
- B.
The DNA polymerases require a preexisting strand with a nucleotide having a 3'-OH.
- C.
The DNA polymerases require a preexisting strand with a nucleotide having a 5'-OH.
- D.
All of the above are correct
Correct Answer
B. The DNA polymerases require a preexisting strand with a nucleotide having a 3'-OH. -
- 18.
The Klenow fragment of E. coli DNA polymerase I can be used to add nucleotides to a DNA with staggered ends if the ends...
- A.
Are recessed with 5'-phosphates
- B.
Are recessed with 3'-OHs
- C.
Are extended with 5'-phosphates
- D.
Are extended with 3'-OHs
Correct Answer
B. Are recessed with 3'-OHs -
- 19.
Which E. coli DNA polymerase is responsible for the repair of DNA?
- A.
DNA polymerase I
- B.
Klenow fragment
- C.
DNA polymerase II
- D.
DNA polymerase III
Correct Answer
B. Klenow fragment -
- 20.
The Klenow fragment from E.coli DNA polymerase contains all of the following except (pg 993)
- A.
DNA polymerase activity
- B.
5'-3' exonuclease activity
- C.
The large fragment from the C-terminal end of the polymerase
- D.
3'-5' exonuclease activity
Correct Answer
B. 5'-3' exonuclease activity -
- 21.
E. coli replication on the lagging strand (pg 1002)
- A.
Is primarily carried out by DNA polymerase I
- B.
Is synthesized continuously
- C.
This DNA strand is synthesized in a 3'-5' direction of synthesis
- D.
Is initially synthesized as Okazaki fragments
Correct Answer
D. Is initially synthesized as Okazaki fragments -
- 22.
Which one of the following characteristics ls not associated with E.coli primase?
- A.
It syntheslzes the RNA primer in DNA repllcation.
- B.
It is a specific RNA polymerase
- C.
It is essential for DNA replication
- D.
It is also a gyrase
Correct Answer
D. It is also a gyrase -
- 23.
The polymerase which plays a similar role as that of Prokaryotic Pol III in eukaryotic replication is (pg 1006)
- A.
DNA polymerase alpha
- B.
DNA polymerase alpha
- C.
DNA polymerase delta
- D.
DNA polymerase alpha
Correct Answer
C. DNA polymerase delta -
- 24.
Which is not a property of E.coli DNA polymerase III?
- A.
It is responsible for incorporating most of the nucleotides in the lagging strand
- B.
It synthesizes most of the leading strand prior to aiding in the synthesis of the lagging strand
- C.
It contains a 3' to 5' exonuclease activity
- D.
It synthesizes the leading strand and the lagging strand at the same time
Correct Answer
B. It synthesizes most of the leading strand prior to aiding in the synthesis of the lagging strand -
- 25.
Which of the following enzymes possesses a 5' to 3' exonuclease activity?
- A.
Primase
- B.
DNA polymerase I
- C.
DNA polymerase III
- D.
DNA gyrase
Correct Answer
B. DNA polymerase I -
- 26.
Which statement is INCORRECT about E. coli DNA polymerase I? (pg 991)
- A.
The cell contains more molecules of DNA pol I than DNA pol III
- B.
DNA pol I is a single polypeptide with three domains
- C.
DNA pol I is involved in the repair of UV damaged DNA
- D.
DNA pol I has a larger turnover number than DNA pol III
Correct Answer
D. DNA pol I has a larger turnover number than DNA pol III -
- 27.
Which statement is INCORRECT about E. coli DNA ligase?
- A.
It functions int he replication but not in the repair of DNA
- B.
It forms a "DNA-AMP" covalent complex during the reaction it catalyzes
- C.
It can seal nicks in the dsDNA as long as the adjacent nucleotides surrounding the nick contain a free 3' hydroxyl and a 5' phosphate
- D.
It catalyzes the formation of a phosphoester bond in dsDNA
Correct Answer
A. It functions int he replication but not in the repair of DNA -
- 28.
Which E. coli enzyme has the greatest frequency of incorporating the incorrect nucleotide into its nucleic acid polymer?
- A.
RNA polymerase
- B.
DNA polymerase I
- C.
DNA polymerase III
- D.
Klenow fragment from DNA polymerase I
Correct Answer
A. RNA polymerase -
- 29.
Which one of the following DNA sequences is palindromic?
- A.
GCGCGCGCGCGC / CGCGCGCGCGCG
- B.
GGGGGGGGCGA / CCCCCCCCGCC
- C.
ATGCATGC / TACGTACG
- D.
GGCCGGCCGG / CCGGCCGGCC
Correct Answer
A. GCGCGCGCGCGC / CGCGCGCGCGCG -
- 30.
The synthesis of the DNA strand is thermodynamically driven by
- A.
The dNTP hydrolysis to produce the incorporated NMP and the PPi
- B.
The nature of the strong connecting bonds formed int he linked nucleotides
- C.
The dNTP hydrolysis and the subsequent hydrolysis of PPi by pyrophosphatase
- D.
The inherent stability of the synthesized DNA polynucleotide
Correct Answer
B. The nature of the strong connecting bonds formed int he linked nucleotides -
- 31.
The promoter site is
- A.
The start base for transcription in DNA
- B.
The binding site in DNA for regulatory proteins which stimulate or further promote the level of transcription
- C.
The site on DNA at which RNA polymerase binds to initiate transcription
- D.
The general region of DNA downstream from the start site.
Correct Answer
B. The binding site in DNA for regulatory proteins which stimulate or further promote the level of transcription -
- 32.
Which of the following factors or subunits is contained within the core RNA polymerase in prokaryotic organisms? (pg 1016)
- A.
Alpha, beta, and beta'
- B.
Alpha, beta, beta', and gamma holoenzyme
- C.
TFIID, SP1 and enhancer protein
- D.
TF IIIA, TFIIIB, and TFIIIC
Correct Answer
A. Alpha, beta, and beta' -
- 33.
The subunit in E. coli RNA polymerase which is required fro recognition of the promoter sequence is
- A.
Alpha
- B.
Beta
- C.
Beta prime
- D.
Gamma
Correct Answer
D. Gamma -
- 34.
RNA polymerase II (pg 102)
- A.
Is located int he nucleolus and transcribes the major ribosomal RNA genes
- B.
Is located in the nucleoplasm (general nucleus) and transcribes protein-encoding genes
- C.
Transcribes the 5S RNA genes
- D.
Transcribes RNAs associated with mRNA processing (SNRP's)
Correct Answer
D. Transcribes RNAs associated with mRNA processing (SNRP's) -
- 35.
The lac operon includes (pg 1031)
- A.
The structural genes for lacZ, lacY, and lacA
- B.
The three structural genes listed in (a) and the operator and promoter sequences for these genes
- C.
All elements listed in (b) and the lacI gene
- D.
All elements in (c) and the promoter for lacI
Correct Answer
D. All elements in (c) and the promoter for lacI -
- 36.
Which statemnt is INCORRECT aobut enhancer sequences in eukaryotes (pg 1015)
- A.
They can occur within a gene
- B.
They can occur either 3' or 5' to the gene
- C.
They can be in either orientation
- D.
They can be placed at different distances from the gene without changing their influence
Correct Answer
A. They can occur within a gene -
- 37.
Which statement is INCORRECT about eh synthesis of RNA by RNA polymerase in E. coli?
- A.
A
- B.
B
Correct Answer
A. A -
- 38.
Prokaryotic genes have conserved DNA sequences centered around -10 and -35 before the initiation point of transcription. THese regions contain a binding site for:
- A.
The rho protein
- B.
DNA polymerase
- C.
Ribosomes
- D.
RNA polymerase holoenzyme
Correct Answer
D. RNA polymerase holoenzyme -
- 39.
A strain of E. coli produces the same high level of beta-galactosidase regardless of the concentration of lactose in the medium. This strain most liekly contains a deletion mutation in the gene (Note: you may assume that the deletion mutation causes loss of normal function) (pg 10)
- A.
Lac 1
- B.
Lac 2
- C.
Gene coding for the gamma-subunit of RNA polymerase
- D.
Gene encoding the catabolite activator protein
Correct Answer
A. Lac 1 -
- 40.
Which statement is CORRECT about RNA polymerase in E. coli?
- A.
It transcribes the DNA sense strand
- B.
It transcribes the DNA antisense strand
- C.
It transcribes only protein genes
- D.
It translates DNA into protein
Correct Answer
B. It transcribes the DNA antisense strand -
- 41.
Which statement is INCORRECT about a typical E. coli promoter? (pg 1019)
- A.
It consists of approximately 60 bp of dsDNA
- B.
It is recognized and bound by the gamma-subunit of RNA polymerase holoenzyme
- C.
The base sequences centered at -10 of various promoters are not identical but almost always differ by two or fewer bases from the consensus sequence
- D.
It contains a "GC rich" consensus seuqence centered at =10 called the Pribnow box
Correct Answer
D. It contains a "GC rich" consensus seuqence centered at =10 called the Pribnow box -
- 42.
The reason why DNA does not contain uracyl is because
- A.
Uracyl forms unstable hydrogen bonds
- B.
Cytosine spontaneously becomes uracyl
- C.
Thymine can do the same function as uracyl
- D.
Uracyl can only from glycosidic bonds with ribose
Correct Answer
B. Cytosine spontaneously becomes uracyl -
- 43.
In the Sanger's DNA sequencing method of the following oligonucleotide, what will be the sequence read form the gel? AATCGATTCGGGCTATA. The primer is 4 nucleotides long. (pg 359)
- A.
AATCGATTCGGGCTATA
- B.
TTAGCTAAGCCCGATAT
- C.
The primer strand plus TTAGCTAAGCCCG
- D.
TTAGCTAAGCCCG
Correct Answer
B. TTAGCTAAGCCCGATAT -
- 44.
An enzyme that changes the shape of supercoiled DNA without changing its linking number is (pg 376 or 576)
- A.
Gyrase
- B.
Topoisomerase II
- C.
Helicase
- D.
Topoisomerase I
Correct Answer
C. Helicase -
- 45.
Which polymerase has the highest processivity?
- A.
DNA Polymerase III
- B.
DNA Polymerase gamma
- C.
DNA Polymerase delta
Correct Answer
C. DNA Polymerase delta -
- 46.
The genes for the enzymes needed int he synthetic pathway of a biomolecule usually are found int he form of
- A.
Gene families
- B.
An operon
- C.
Housekeeping genes
- D.
Oncogenes
Correct Answer
B. An operon -
- 47.
The term "upstream" means (p 1019)
- A.
Any place int he DNA after the promoter site
- B.
Any place in the DNA at the noncoding region
- C.
A consensus sequence that goes from 3' to 5'
- D.
Any region in the DNA that is closer to the 5' end
Correct Answer
D. Any region in the DNA that is closer to the 5' end -
- 48.
Telomerase is an enzyme that (p 1008)
- A.
Decreases the size of the telomeres
- B.
Extends the 3' end of the telomeres lagging strand
- C.
It is not found in vertebrates
- D.
Uses a DNA primer to enxtend DNA
Correct Answer
B. Extends the 3' end of the telomeres lagging strand -
- 49.
A strain of E.coli produces the same high level of beta-galactosidase regardless of concentration of lactose in the medium. This strain most likely contains a deltion mutation int he gene (Note: you may assume that deletion mutation causes normal function
- A.
Lac I
- B.
Lac 2
- C.
Gene coding for the gamma-subunit of RNA polymerase
- D.
Gene encoding the catabolite activator protein
Correct Answer
A. Lac I -
- 50.
Which statement is INCORRECT about RNA polymerase in E. coli?
- A.
It does not contain a 3' or 5' exonuclease activity
- B.
Its holoenzyme contains four different subunits organized as alpha beta beta prime, gamma
- C.
It synthesizes mRNA, tRNA, rRNA as well as the RNA primers during replication
- D.
It uses the nucleotides UTP, GTP, ATP, and CTP as substrates
Correct Answer
C. It synthesizes mRNA, tRNA, rRNA as well as the RNA primers during replication -
Quiz Review Timeline +
Our quizzes are rigorously reviewed, monitored and continuously updated by our expert board to maintain accuracy, relevance, and timeliness.
-
Current Version
-
Dec 02, 2012Quiz Edited by
ProProfs Editorial Team -
Oct 31, 2012Quiz Created by
Andreazza
- Aeronautics Quizzes
- Aerospace Quizzes
- Agricultural Science Quizzes
- Astrology Quizzes
- Astronomy Quizzes
- Atom Quizzes
- Biology Quizzes
- Biomechanics Quizzes
- Biostatistics Quizzes
- Biotechnology Quizzes
- Botany Quizzes
- Branches Of Science Quizzes
- Cytology Quizzes
- Easy Science Quizzes
- Ecology Quizzes
- Electrical Quizzes
- Embryology Quizzes
- Endocrinology Quizzes
- Engineering Quizzes
- Environmental Science Quizzes
- Epidemiology Quizzes
- Experiment Quizzes
- Forestry Quizzes
- Fossil Quizzes
- Gas Quizzes
- General Science Quizzes
- Genetics Quizzes
- Histology Quizzes
- Human Biology Quizzes
- Integrated Science Quizzes
- Invention Quizzes
- Library Science Quizzes
- Lighting Quizzes
- Liquid Quizzes
- Marine Biology Quizzes
- Microbiology Quizzes
- Nature Quizzes
- Neuroscience Quizzes
- Nuclear Science Quizzes
- Oceanography Quizzes
- Physics Quizzes
- Psychology Quizzes
- Science And Technology Quizzes
- Science Glossary Quizzes
- Science Knowledge Quizzes
- Science Practice Quizzes
- Scientific Method Quizzes
- Scientific Notation Quizzes
- Soil Science Quizzes
- Solar System Quizzes
- Solid Quizzes
- Zoology Quizzes
Back to top