Microbiology Lab Exam #1

34 Questions  I  By Caitlin.mumaw
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Microbiology Quizzes & Trivia
Quiz to study for McNeely's Lab Exam #1:Includes: oil immersionaseptic techniquestreaking for isolationgram stainingacid-fast stainingspecial staining

  
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  • 1. 
    What two parts of the microscope are used to carry or support the microscope?

  • 2. 
    What are the three optical parts of a microscope?

  • 3. 
    Red colored objective is called what?
    • A. 

      Scanning

    • B. 

      Low-dry

    • C. 

      Oil immersion


  • 4. 
    What total magnification does the scanning objective have?
    • A. 

      4

    • B. 

      40

    • C. 

      10


  • 5. 
    What is the real name for the blue-rimmed objective?
    • A. 

      High-dry

    • B. 

      Low-dry

    • C. 

      Scanning


  • 6. 
    What is the real name for the yellow-rimmed objective?
    • A. 

      Low-dry

    • B. 

      High-dry

    • C. 

      Oil immersion


  • 7. 
    What is the real name of the white-rimmed objective?
    • A. 

      Scanning

    • B. 

      Low-dry

    • C. 

      Oil immersion


  • 8. 
    What is the total magnification for low-dry?
    • A. 

      40

    • B. 

      1000

    • C. 

      100


  • 9. 
    What is the total magnification for high-dry?
    • A. 

      400

    • B. 

      40

    • C. 

      1000


  • 10. 
    What is the total magnification for oil immersion?
    • A. 

      10

    • B. 

      400

    • C. 

      1000


  • 11. 
    What are the functions of the abbe condenser?
    • A. 

      Shuts off the light

    • B. 

      Collects light

    • C. 

      Condenses light

    • D. 

      Alternate light source

    • E. 

      Only light source


  • 12. 
    What is the function of the iris diaphragm?
    • A. 

      Turns light on, controls light

    • B. 

      Adjustment for light coming through the abbe condenser; want it to ideally be in the middle; when looking through lens, light shouldn't be blinding.


  • 13. 
    Four microscopic morphologies of bacteria?

  • 14. 
    Parfocal lenses allow for us to be able to go between objectives with little adjustment of ___.
    • A. 

      Iris diaphragm

    • B. 

      Abbe condenser

    • C. 

      Fine focus


  • 15. 
    What is the purpose of oil immersion?
    • A. 

      To use oil to replace air and to prevent refraction at a glass-air interface.

    • B. 

      To use oil to replace air to prevent absorption of bacteria


  • 16. 
    How do you get total magnification (equation)

  • 17. 
    What is the appropriate order of clean up?1. clean stage with used alcohol swab2. clean ocular lenses with alcohol swab and take lense paper to clean/wipe of oculars, polish3. clean yellow, blue, white objectives
    • A. 

      3, 2, 1

    • B. 

      1, 2, 3,

    • C. 

      2, 3, 1


  • 18. 
    Why is sheep's blood used as media?
    • A. 

      Non-fastidious bacteria

    • B. 

      Fastidious bacteria

    • C. 

      Requires extra nutirents


  • 19. 
    What are five characteristics of bacterial colonial morphologies?
    • A. 

      Differences in size

    • B. 

      Shape

    • C. 

      Color or pigment

    • D. 

      Weight

    • E. 

      Toxicity

    • F. 

      Texture

    • G. 

      Density


  • 20. 
    Why should a petri dish not be left open for an extended period of time?

  • 21. 
    What does streaking for isolation mean?

  • 22. 
    Purpose of inverting plates during incubation?
    • A. 

      To not allow bacteria to unnecessarily clump or to let it dissolve into agar

    • B. 

      To prevent buildup of condensation that would ruin the culture


  • 23. 
    What is normal flora?
    • A. 

      Air contamination

    • B. 

      Buildup of condensation

    • C. 

      Grows on different mucosal surfaces of the body


  • 24. 
    Four places normal flora exists
    • A. 

      GI tract

    • B. 

      Vagina

    • C. 

      Armpit

    • D. 

      Throat, mouth

    • E. 

      Nose

    • F. 

      Sinuses


  • 25. 
    What color is serratia at room temperature?

  • 26. 
    What two things should you always do to enhance isolation of bacteria?
    • A. 

      Flame and cool needle after primary inoculum

    • B. 

      Firm touch, don't scoop

    • C. 

      Scoop up bacteria


  • 27. 
    What is media?
    • A. 

      Where bacteria is taken from originally

    • B. 

      Broth or agar based

    • C. 

      "base" for bacteria to be added to


  • 28. 
    What and where is agar from?
    • A. 

      Solid form of media

    • B. 

      Liquid form of media

    • C. 

      Comes from seaweed

    • D. 

      Comes from pebbles


  • 29. 
    What are ways to tell if media is sterile?
    • A. 

      Run controls

    • B. 

      Inoculate with bacteria

    • C. 

      No floaters in agar

    • D. 

      No growth in broth


  • 30. 
    Why are culture tubes held at an angle?

  • 31. 
    What are two possible errors which could have occurred if you find your subcultures had no growth after incubation for 48 hours?
    • A. 

      Not a sterile plate

    • B. 

      Forgetting to inoculate

    • C. 

      Could have killed bacteria with heat from un-cooled needle


  • 32. 
    What are things to do while using a bunsen burner?
    • A. 

      Have hair down and near bunsen burner

    • B. 

      Never lean over burner with flame on

    • C. 

      Light match before turning gas flow on


  • 33. 
    What does a positive acid-fast stain look like?

  • 34. 
    What group of organisms are considered acid-fast?

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