Quiz Online Uas Biologi Molekuler Farmasi 2008/2009

1. Which of the following is a technique that is carried out by placing the nucleic acid samples in the gel and applying a potential difference across it.

Duplex DNA is heat denatured to give single strands

The two oligonucleotide primers are annealed to the target DNA

A thermostable DNA polymerase adds dNTPs onto the 3' ends of the two annealed primers

Detecting fragments in an agarose gel that are complementary to a given DNA sequence

2. A coding region within a discontinuous gene.

RNA is usually double-stranded, but DNA is usually single-stranded.

RNA lacks the base thymine (which is found in DNA) and has uracil instead

RNA contains three different nucleotides, but DNA contains four different nucleotides.

RNA has the sugar deoxyribose, but DNA has the sugar ribose

3. A useful method for genomic profiling and involves using a single primer at low stringency.

Genomics

Proteome

Genomic library

CDNA library

4. The conversion of RNA to DNA requires a special enzyme called ______________

Nested PCR

Inverse PCR

RAPD-PCR

RT-PCR

5. Which of the following is a colourless substrate for β-galactosidase.

Southern hybridization

Northern hybridization

PCR

Western hybridization

6. Insertion of a fragment of DNA into a cloning vector, and subsequent propagation of the recombinant DNA molecule in a host organism.

Shotgun cloning

CDNA cloning

Insertional inactivation

DNA cloning

7. The basic unit of organisation in biological systems.

The central dogma

Operon

DNA cloning

Genetic Engineering

8. A vector that can replicate in the cells of more than one organism (e.g. in Escherichia coli and in yeast).

Nested PCR

Inverse PCR

RAPD-PCR

RT-PCR

9. The introduction of purified virus DNA molecules into any living cell.

Hybrid-arrest translation

Hybrid-release translation

Insertional inactivation

Hybrid-select translation

10. Which of the following is a synthetic, double-stranded oligonucleotide used to attach sticky ends to a blunt-ended molecule.

Southern hybridization

Northern hybridization

PCR

Western hybridization

11. Which of the following is a short, synthetic, single-stranded oligonucleotide used in DNA sequencing or PCR

Primer

Linker

Selectable marker

Template

12. A cloning strategy that involves the insertion of random fragments of a large DNA molecule into a vector, resulting in a large number of different recombinant DNA molecules.

Primer

Linker

Selectable marker

Template

13. A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA

Vector

Electroporation

Transformation

Transfection

14. A technique which involves the transfer of electrophoretically separated protein molecules to membranes

Vector

Primer

Linker

Selectable marker

15. Using mRNA as the template is a common variant of the basic PCR, this technique known as _________.

PUC18

Yip

Ti Plasmid

BAC

16. Which of the following is often used to describe a set of clones representing the entire genome of an organism?

Transcriptase

RNA-dependent DNA polymerase

DNA polymerase

Template-dependent DNA polymerase

17. A technique used for detection of a specific RNA molecule against a background of many other RNA molecules.

Southern hybridization

Northern hybridization

Dot blotting

Western hybridization

18. Which of the following enzymes are found in bacterial cells, where they act as a ‘protection’ system for bacteria in that they hydrolyse exogenous DNA that is not methylated by the host modification enzyme?

PCR . .

SAGE

PAGE

Northern Blotting

19. A high-capacity cloning vector consisting of the λ cos site inserted into a plasmid.

Restriction enzyme

Alkaline phosphatase

Reverse transcriptase

DNA Ligase

20. A cell that has been introduced by exogenous DNA

Restriction enzymes

Alkaline phosphatase

Reverse transcriptase

DNA Ligase

21. Transformation of competent cells is carried out by mixing the plasmid DNA with the cells, incubating on ice for 20--30 min, and then giving a brief heat shock at __________.

Transformant

DNA recombinant

E. coli recombinant

Plasmid recombinant

22. Which of the following is a vector that can be used to clone large fragments of DNA (in excess of several hundred kb).

42◦C

52◦C

72◦C

92◦C

23. In the gene cloning technique, the enzyme _____________ is used to breaks phosphodiester bonds within a nucleic acid molecule.

CaCl2

RNase

Ethanol

Phenol

24. Which of the following is a technique for determining the order of nucleotides in a DNA molecule

Southern hybridization

Northern hybridization

PCR

Western hybridization

25. A method used to indentify the polypeptide coded by a cloned gene which is immobilised and used to select the clone-specific mRNA from the total mRNA preparation.

Hybrid-arrest translation

Hybrid-release translation

Insertional inactivation

Hybrid-select translation

26. Which of the following is a gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector.

Primer

Linker

Selectable marker

Template

27. A single-stranded polynucleotide (or region of a polynucleotide) that directs synthesis of a complementary polynucleotide.

Primer

Linker

Selectable marker

Template

28. A technique was invented by Kary Mullis and his colleagues in the 1980s.

Shotgun cloning

CDNA cloning

Insertional inactivation

DNA cloning

29. A method used to indentify the polypeptide coded by a cloned gene which blocks the mRNA and prevents its translation when placed in a system containing all the components of the translational machinery.

Southern hybridization

Northern hybridization

PCR

Western hybridization

30. Separation of the DNA fragments created in sequencing reactions is achieved by ________.

PCR

SAGE

PAGE

DNA sequencing

31. Which of the following is the enzyme that is used mainly for copying mRNA molecules in the preparation of cDNA (complementary or copy DNA) for cloning?

PB322

Virus

Plasmid

Cosmid

32. Which of the following is an enzyme that removes phosphate groups from the 5′ ends of DNA molecules.

Restriction enzyme

Alkaline phosphatase

Reverse transcriptase

DNA Ligase

33. An enzyme that adds one or more nucleotides to the 3′ end of a DNA molecule.

Restriction enzyme

Alkaline phosphatase

Reverse transcriptase

Terminal transferase

34. An enzyme that synthesizes phosphodiester bonds as part of DNA replication, repair and recombination processes.

Restriction enzyme

DNA Ligase

Alkaline phosphatase

Terminal transferase

35. Which of the following is plasmid that are found in Agrobacterium tumifaciens and are responsible for the formation of crown gall tumors in plants?

PUC18

Yip

Ti Plasmid

BAC

36. Which of the following is plasmid that contains genes for ampicillin resistance (Ap^r) and tetracycline resistance (Tc^r)

Cosmid

Shuttle vector

Phagemid

Yip

37. If nucleic acid samples are not subjected to electrophoresis but are spotted onto the filters, the technique is known as ___________

PUC18

PBR322

YEp

Yip

38. This technique involves the transfer of electrophoresed protein bands from a polyacrylamide gel on to a membrane of nitrocellulose or nylon.

Southern hybridization

Northern hybridization

PCR

Western hybridization

39. The term refers to the population of mRNA at any given point in a cell’s life.

Genome

Exon

Proteome

Transcriptome

40. The nucleotide sequence to which a repressor protein binds to prevent transcription of a gene or operon

2′-deoxyribose

A nitrogenous base

A phosphate group

Ribose

41. Which of the following is an accurate statement concerning the differences between DNA and RNA?

Promoter

Repressor

Operator

Open reading frame (ORF)

42. The flow of genetic information is from DNA to RNA to protein, via the processes of transcription and translation. This concept is known as ____________

Operator

Operon

Enhancer

Promoter

43. Following cell disruption, cell preparations can be deproteinised by using __________

Gene

Operon

Codon

Cell

44. Which of the following is NOT a component of nucleotide of RNA?

Phosphodiester bond

Hydrogen bond

Hydrophobic

β-N-glycosidic bond

45. The nucleotide sequence, upstream of a gene, to which RNA polymerase binds in order to initiate transcription

Exon

Operon

Codon

Promoter

46. All of the following are the basic mechanis steps of PCR, EXCEPT_________

DNA polymerase

Polynucleotide kinase

Ligase

Endonuclease

47. Insertion of a fragment of DNA into a cloning vector, and subsequent propagation of the recombinant DNA molecule in a host organism.

PCR

SAGE

PAGE

DNA sequencing

48. Which of the following is a highly stable synthetic analog of lactose

Chromogenic substrate

X-gal

LacZ

IPTG

49. A technique that results in exponential amplification of a selected region of a DNA molecule.

Chromogenic substrate

X-gal

LacZ

IPTG

50. A cloning strategy whereby insertion of a new piece of DNA into a vector inactivates a gene carried by the vector.

Shotgun cloning

CDNA cloning

Insertional inactivation

DNA cloning

Quiz Online Uas Biologi Molekuler Farmasi 2008/2009

1. Which of the following is a technique that is carried out by placing the nucleic acid samples in the gel and applying a potential difference across it.

2.

What first name or nickname would you like us to use?

2. A coding region within a discontinuous gene.

3. A useful method for genomic profiling and involves using a single primer at low stringency.

4. The conversion of RNA to DNA requires a special enzyme called ______________

5. Which of the following is a colourless substrate for β-galactosidase.

6. Insertion of a fragment of DNA into a cloning vector, and subsequent propagation of the recombinant DNA molecule in a host organism.

7. The basic unit of organisation in biological systems.

8. A vector that can replicate in the cells of more than one organism (e.g. in Escherichia coli and in yeast).

9. The introduction of purified virus DNA molecules into any living cell.

10. Which of the following is a synthetic, double-stranded oligonucleotide used to attach sticky ends to a blunt-ended molecule.

11. Which of the following is a short, synthetic, single-stranded oligonucleotide used in DNA sequencing or PCR

12. A cloning strategy that involves the insertion of random fragments of a large DNA molecule into a vector, resulting in a large number of different recombinant DNA molecules.

13. A DNA molecule that is able to replicate inside a host cell and therefore can be used to clone other fragments of DNA

14. A technique which involves the transfer of electrophoretically separated protein molecules to membranes

15. Using mRNA as the template is a common variant of the basic PCR, this technique known as _________.

16. Which of the following is often used to describe a set of clones representing the entire genome of an organism?

17. A technique used for detection of a specific RNA molecule against a background of many other RNA molecules.

18. Which of the following enzymes are found in bacterial cells, where they act as a ‘protection’ system for bacteria in that they hydrolyse exogenous DNA that is not methylated by the host modification enzyme?

19. A high-capacity cloning vector consisting of the λ cos site inserted into a plasmid.

20. A cell that has been introduced by exogenous DNA

21. Transformation of competent cells is carried out by mixing the plasmid DNA with the cells, incubating on ice for 20--30 min, and then giving a brief heat shock at __________.

22. Which of the following is a vector that can be used to clone large fragments of DNA (in excess of several hundred kb).

23. In the gene cloning technique, the enzyme _____________ is used to breaks phosphodiester bonds within a nucleic acid molecule.

24. Which of the following is a technique for determining the order of nucleotides in a DNA molecule

25. A method used to indentify the polypeptide coded by a cloned gene which is immobilised and used to select the clone-specific mRNA from the total mRNA preparation.

26. Which of the following is a gene carried by a vector and conferring a recognizable characteristic on a cell containing the vector or a recombinant DNA molecule derived from the vector.

27. A single-stranded polynucleotide (or region of a polynucleotide) that directs synthesis of a complementary polynucleotide.

28. A technique was invented by Kary Mullis and his colleagues in the 1980s.

29. A method used to indentify the polypeptide coded by a cloned gene which blocks the mRNA and prevents its translation when placed in a system containing all the components of the translational machinery.

30. Separation of the DNA fragments created in sequencing reactions is achieved by ________.

31. Which of the following is the enzyme that is used mainly for copying mRNA molecules in the preparation of cDNA (complementary or copy DNA) for cloning?

32. Which of the following is an enzyme that removes phosphate groups from the 5′ ends of DNA molecules.

33. An enzyme that adds one or more nucleotides to the 3′ end of a DNA molecule.

34. An enzyme that synthesizes phosphodiester bonds as part of DNA replication, repair and recombination processes.

35. Which of the following is plasmid that are found in Agrobacterium tumifaciens and are responsible for the formation of crown gall tumors in plants?

36. Which of the following is plasmid that contains genes for ampicillin resistance (Apr) and tetracycline resistance (Tcr)

37. If nucleic acid samples are not subjected to electrophoresis but are spotted onto the filters, the technique is known as ___________

38. This technique involves the transfer of electrophoresed protein bands from a polyacrylamide gel on to a membrane of nitrocellulose or nylon.

39. The term refers to the population of mRNA at any given point in a cell’s life.

40. The nucleotide sequence to which a repressor protein binds to prevent transcription of a gene or operon

41. Which of the following is an accurate statement concerning the differences between DNA and RNA?

42. The flow of genetic information is from DNA to RNA to protein, via the processes of transcription and translation. This concept is known as ____________

43. Following cell disruption, cell preparations can be deproteinised by using __________

44. Which of the following is NOT a component of nucleotide of RNA?

45. The nucleotide sequence, upstream of a gene, to which RNA polymerase binds in order to initiate transcription

46. All of the following are the basic mechanis steps of PCR, EXCEPT_________

47. Insertion of a fragment of DNA into a cloning vector, and subsequent propagation of the recombinant DNA molecule in a host organism.

48. Which of the following is a highly stable synthetic analog of lactose

49. A technique that results in exponential amplification of a selected region of a DNA molecule.

50. A cloning strategy whereby insertion of a new piece of DNA into a vector inactivates a gene carried by the vector.

36. Which of the following is plasmid that contains genes for ampicillin resistance (Ap^r) and tetracycline resistance (Tc^r)