Microbiology Questions
A review for microbiology in order to get an A for the final exam
- 1.
Who proved that microorganisms cause disease?
- A.
Pasteur
- B.
Van Leeuwenhoek
- C.
Koch
- D.
Hooke
Correct Answer
C. Koch -
- 2.
Nitrogen bases are building blocks of:
- A.
Lipids
- B.
Deoxyribose
- C.
Nucleotides
- D.
Ribosomes
- E.
None of these
Correct Answer
C. Nucleotides -
- 3.
Proteins are formed by amino acids connected by:
- A.
Alpha 1,4-glycosidic bond
- B.
Phosphodiester linkage
- C.
Hydrogen bonds
- D.
Peptide bonds
Correct Answer
D. Peptide bonds -
- 4.
Which of the following is not a kingdom in the five-kingdom system?
- A.
Protista
- B.
Algae
- C.
Plant
- D.
Animal
- E.
Monera
Correct Answer
B. Algae -
- 5.
The best use of a negative stain is:
- A.
To determine cell shape
- B.
To see endospore
- C.
To determine Gram reaction
- D.
To determine motility
Correct Answer
A. To determine cell shape -
- 6.
We discussed two processes for synthesizing ATP. Which process requires proton gradient across the membrane?
- A.
Substrate level phosphorylation
- B.
Oxidative level phosphorylation
Correct Answer
B. Oxidative level phosphorylation -
- 7.
Place the following steps in the correct sequence: 1 - Staining; 2- Making a smear; 3 - Fixing
- A.
1-2-3
- B.
1-3-2
- C.
3-2-1
- D.
2-3-1
Correct Answer
D. 2-3-1 -
- 8.
Which of the following chemicals is found only in the outer membrane of Gram-negative bacteria?
- A.
Plasmid
- B.
Teichoic acid
- C.
Flagella
- D.
Lipopolysaccharide
- E.
Peptidoglycan
Correct Answer
D. Lipopolysaccharide -
- 9.
Identify the FALSE statement.
- A.
The cell wall of G+ is much thicker than G-
- B.
In Gram stain, G- are blue (purple) and G+ are pink (red)
- C.
G+ and NOT G- contain teichoic acid in their cell wall
- D.
In Gram stain, G+ are blue (purple) and G- are pink (red)
Correct Answer
B. In Gram stain, G- are blue (purple) and G+ are pink (red) -
- 10.
Which of the following is one of the hallmarks of a cell?
- A.
Self sufficient
- B.
Disease causing
- C.
Having cell wall
- D.
Self replicating
- E.
Presence of RNA and ribosome in the ell
Correct Answer
D. Self replicating -
- 11.
All of the following statements are true of gram-positive cells except which one?
- A.
Gram positive cells have more peptidoglycan
- B.
Gram positive cells have endotoxin
- C.
Gram positive cells are sensitive to penicillin
- D.
Gram positive cells are decolorized by alcohol-acetone
Correct Answer
B. Gram positive cells have endotoxin -
- 12.
Which of the following statements are about a gram-negative cell wall is not true?
- A.
It has teichoic acids
- B.
It contains an endotoxin
- C.
It protects the cell from osmotic lysis
- D.
It maintains the shape of the cell
Correct Answer
A. It has teichoic acids -
- 13.
Which of the following is not a kingdom in the five-kingdom system?
- A.
Animal
- B.
Fungi
- C.
Monera
- D.
Plant
- E.
Virus
Correct Answer
E. Virus -
- 14.
Which of the following chemical bonds is the strongest?
- A.
Ionic bond
- B.
Covalent bond
- C.
Hydrogen Bond
- D.
They all have the same strength
Correct Answer
B. Covalent bond -
- 15.
Bacterial cell membrane DOES NOT contain:
- A.
Phospholipids
- B.
Proteins
- C.
Glycoproteins
- D.
Lipids
- E.
Nucleic acids
Correct Answer
E. Nucleic acids -
- 16.
All of the following can be found in mitochondria except:
- A.
Circular DNA
- B.
Ribosome
- C.
ATP-generating mechanism
- D.
Electron transport system
- E.
Cell wall
Correct Answer
E. Cell wall -
- 17.
Which of the following is not a characteristic of a Bacteria?
- A.
All are unicellular.
- B.
All are pathogenic.
- C.
Cell walls are composed of peptidoglycan.
- D.
Most possess cell walls.
Correct Answer
B. All are pathogenic. -
- 18.
This microscope uses an ultraviolet light source.
- A.
Dark field microscope
- B.
Fluorescence microscope
- C.
Electron microscope
- D.
Compound light microscope
Correct Answer
B. Fluorescence microscope -
- 19.
Which of the following is considered an endotoxin?
- A.
Teichoic acid
- B.
Lipopolysaccharide
- C.
Periplasm
- D.
Murein
- E.
Peptidoglycan
Correct Answer
B. Lipopolysaccharide -
- 20.
Bacteria differ from viruses because bacteria
- A.
Have cells
- B.
Have DNA and RNA
- C.
Can live without a host
- D.
All of these
Correct Answer
D. All of these -
- 21.
These molecules are the primary building blocks of the plasma membrane in cells
- A.
Carbohydrates
- B.
Nucleic acids
- C.
Proteins
- D.
Lipids
Correct Answer
D. Lipids -
- 22.
Which of the following does not contribute to the hydrophobic interaction?
- A.
Ionic interaction between hydrophobic molecules
- B.
Proximity of hydrophobic molecules
- C.
Increase entropy
- D.
Exclusion of water
Correct Answer
A. Ionic interaction between hydrophobic molecules -
- 23.
These are the primary molecules making up plasma membrane in cells
- A.
Nucleic acids
- B.
Carbohydrates
- C.
Proteins
- D.
Lipids
Correct Answer
D. Lipids -
- 24.
This is not a major bioelement
- A.
Sulfur
- B.
Nitrogen
- C.
Hydrogen
- D.
Magnesium
- E.
Phosphate
Correct Answer
D. Magnesium -
- 25.
A protein's function depends upon its unique conformation. Which level of protein folding achieves this conformation?
- A.
Primary structure
- B.
Beta sheet
- C.
Alpha helix
- D.
Secondary structure
- E.
Tertiary structure
Correct Answer
E. Tertiary structure -
- 26.
Which forces/bonds are involved in quaternary structure of proteins?
- A.
Hydrogen bond, S-S bridges, Van der Waals forces, peptide bonds
- B.
Hydrogen bond, S-S bridges, Van der Waals forces, peptide bonds, and hydrophobic interactions
- C.
Hydrogen bond, S-S bridges, peptide bonds
- D.
Hydrogen bond, S-S bridges, Van der Waals forces, hydrophobic interactions
Correct Answer
B. Hydrogen bond, S-S bridges, Van der Waals forces, peptide bonds, and hydrophobic interactions -
- 27.
This is not an internal structure of a bacteria
- A.
Ribosome
- B.
Plasmid
- C.
Fimbriae
- D.
Nucleoid
Correct Answer
C. Fimbriae -
- 28.
You can find nucleotides in
- A.
DNA
- B.
Amino acids
- C.
Fatty acids
- D.
Proteins
- E.
Carbohydrates
Correct Answer
A. DNA -
- 29.
Which are of microbiolgy studies fungi?
- A.
Mycology
- B.
Parasitology
- C.
Biotechnology
- D.
Immunology
- E.
Bioremediation
Correct Answer
A. Mycology -
- 30.
Which of the following compounds has the greatest amount of energy for a cell?
- A.
O2
- B.
Glucose
- C.
CO2
- D.
ATP
- E.
Pyruvate
Correct Answer
B. Glucose -
- 31.
Which of the following is not part of the cell membrane?
- A.
Fatty acids
- B.
Nucleic acids
- C.
Sugars
- D.
Amino acids
Correct Answer
B. Nucleic acids -
- 32.
Cell division occurs in prokaryotes by:
- A.
Budding
- B.
Meiosis
- C.
Binary Fission
- D.
Mitosis
Correct Answer
C. Binary Fission -
- 33.
Which of the following mechanisms requires energy?
- A.
Simple diffusion
- B.
Osmosis
- C.
Facilitated diffusion
- D.
Active transport
Correct Answer
D. Active transport -
- 34.
Cells are made of mostly
- A.
Lipids
- B.
Proteins
- C.
RNA
- D.
DNA
- E.
Carbohydrates
Correct Answer
B. Proteins -
- 35.
What color should a gram-positive organism appear after Gram stain
- A.
Pink
- B.
Red
- C.
Green
- D.
Purple
- E.
Orange
Correct Answer
D. Purple -
- 36.
Eukarya
- A.
Have a membrane around their DNA
- B.
Contain outer membrane and cell membrane
- C.
Consist of only multicellular organisms
- D.
All of these are correct
- E.
None of these are correct
Correct Answer
A. Have a membrane around their DNA -
- 37.
The type of bond holding Na+ and Cl- ions in NaCl is a(n):
- A.
Covalent bond
- B.
Double bond
- C.
Ionic bond
- D.
Hydrogen bond
Correct Answer
C. Ionic bond -
- 38.
A medium that contains cyrstal violet inhibit growth of gram-positive bacteria. The medium is:
- A.
Selective
- B.
Differential
- C.
Enriched
Correct Answer
A. Selective -
- 39.
Loss of electrons means:
- A.
Phosphorylation
- B.
Reduction
- C.
Oxidation
Correct Answer
C. Oxidation -
- 40.
S-S agar inhibits gram-positive bacteria. On this medium, bacteria that ferment lactose to produce red colonies, and bacteria that do not ferment lactose to produce colorless colonies. This medium is:
- A.
Both selective and differential
- B.
Differential
- C.
Enriched
- D.
Selective
Correct Answer
A. Both selective and differential -
- 41.
Which of the following is not a characteristic of cell membrane
- A.
Selective permeability
- B.
Composed of Phospholipid bilayer
- C.
Contains proteins
- D.
Maintain cell shape
- E.
Present in all cells
Correct Answer
D. Maintain cell shape -
- 42.
Which of the following mechanisms generate ATP using an electron transport chain?
- A.
Fermentation
- B.
Glycolysis
- C.
Oxidative phosphorylation
- D.
Substrate level phosphorylation
- E.
Krebs cycle
Correct Answer
C. Oxidative phosphorylation -
- 43.
An example of bioremediation is the use of:
- A.
Bacillus thuringiensis to kill crop-eating insects
- B.
Cowpox virus to vaccinate against smallpox
- C.
Saccharomyces cerevisie to make wine
- D.
Pseudomonas to remove uranium from oil at weapons depots
Correct Answer
D. Pseudomonas to remove uranium from oil at weapons depots -
- 44.
This microscope achieves the highest magnification and greatest resolution.
- A.
Flurorescence microscope
- B.
Phase-contrast microscope
- C.
Electron microscope
- D.
Darkfield microscope
- E.
Compound light microscope
Correct Answer
C. Electron microscope -
- 45.
All of the following organisms are eukaryotes except:
- A.
Plants
- B.
Archaea
- C.
Protozoa
- D.
Fungi
- E.
All of these are eukaryotes
Correct Answer
B. Archaea -
- 46.
Which molecule is composed of a chain of amino acids?
- A.
Carbohydrates
- B.
Proteins
- C.
Lipids
- D.
Nucleic acids
Correct Answer
B. Proteins -
- 47.
The most common solidifying agent added to culture media is:
- A.
Starch
- B.
Silica gel
- C.
Gelatin
- D.
Agar
Correct Answer
D. Agar -
- 48.
The best definition of biotechnology is:
- A.
The use of living organisms to make desired products
- B.
Curing diseases
- C.
The use of microorganisms in sewage treatment
- D.
The development of genetic engineering
Correct Answer
A. The use of living organisms to make desired products -
- 49.
Peptidoglycan is made of mostly:
- A.
Proteins
- B.
Lipids
- C.
Nucleic acids
- D.
Carbohydrates
Correct Answer
D. Carbohydrates -
- 50.
Which of the following is not a product of TCA cycle?
- A.
CO2
- B.
Acetyle-CoA
- C.
NADH
- D.
GTP (ATP)
- E.
FADH
Correct Answer
B. Acetyle-CoA -
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Oct 16, 2014Quiz Edited by
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